Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using 99mTc-HMPAO View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-10-26

AUTHORS

Do Won Hwang, Hongyoon Choi, Su Chul Jang, Min Young Yoo, Ji Yong Park, Na Eun Choi, Hyun Jeong Oh, Seunggyun Ha, Yun-Sang Lee, Jae Min Jeong, Yong Song Gho, Dong Soo Lee

ABSTRACT

Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand in vivo distribution and behavior of exosomes. Here, we developed a simple method for radiolabeling of macrophage-derived exosome-mimetic nanovesicles (ENVs) with 99mTc-HMPAO under physiologic conditions and monitored in vivo distribution of 99mTc-HMPAO-ENVs using SPECT/CT in living mice. ENVs were produced from the mouse RAW264.7 macrophage cell line and labeled with 99mTc-HMPAO for 1 hr incubation, followed by removal of free 99mTc-HMPAO. SPECT/CT images were serially acquired after intravenous injection to BALB/c mouse. When ENVs were labeled with 99mTc-HMPAO, the radiochemical purity of 99mTc-HMPAO-ENVs was higher than 90% and the expression of exosome specific protein (CD63) did not change in 99mTc-HMPAO-ENVs. 99mTc-HMPAO-ENVs showed high serum stability (90%) which was similar to that in phosphate buffered saline until 5 hr. SPECT/CT images of the mice injected with 99mTc-HMPAO-ENVs exhibited higher uptake in liver and no uptake in brain, whereas mice injected with 99mTc-HMPAO showed high brain uptake until 5 hr. Our noninvasive imaging of radiolabeled-ENVs promises better understanding of the in vivo behavior of exosomes for upcoming biomedical application. More... »

PAGES

15636

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URI

http://scigraph.springernature.com/pub.10.1038/srep15636

DOI

http://dx.doi.org/10.1038/srep15636

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https://app.dimensions.ai/details/publication/pub.1014299378

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26497063


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