Super-Resolution Imaging Conditions for enhanced Yellow Fluorescent Protein (eYFP) Demonstrated on DNA Origami Nanorulers View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-11

AUTHORS

Ija Jusuk, Carolin Vietz, Mario Raab, Thorben Dammeyer, Philip Tinnefeld

ABSTRACT

Photostability is one of the crucial properties of a fluorophore which strongly influences the quality of single molecule-based super-resolution imaging. Enhanced yellow fluorescent protein (eYFP) is one of the most widely used versions of fluorescent proteins in modern cell biology exhibiting fast intrinsic blinking and reversible photoactivation by UV light. Here, we developed an assay for studying photostabilization of single eYFP molecules with respect to fast blinking and demonstrated a 6-fold enhanced photostability of single eYFP molecules with a beneficial influence on the blinking kinetics under oxygen removal and addition of aliphatic thiols (dSTORM-buffer). Conjugation to single stranded DNA and immobilization via DNA hybridization on a DNA origami 12 helix bundle in aqueous solution allowed photophyiscal studies of eYFP at the single-molecule level and at close to physiological conditions. The benefit of improved photophysical properties for localization-based super-resolution microscopy is demonstrated and quantitatively characterized by imaging 12 helix bundle DNA origami nanorulers with binding sites at designed distances of 160 and 100 nm and by imaging microtubules in fixed mammalian Vero cells. More... »

PAGES

14075

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep14075

DOI

http://dx.doi.org/10.1038/srep14075

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035340319

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26373229


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cercopithecus aethiops", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luminescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Imaging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nanotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vero Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Braunschweig University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6738.a", 
          "name": [
            "Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig 38106, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jusuk", 
        "givenName": "Ija", 
        "id": "sg:person.01072667764.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072667764.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Braunschweig University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6738.a", 
          "name": [
            "Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig 38106, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vietz", 
        "givenName": "Carolin", 
        "id": "sg:person.01210231443.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210231443.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Braunschweig University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6738.a", 
          "name": [
            "Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig 38106, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raab", 
        "givenName": "Mario", 
        "id": "sg:person.01206452510.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206452510.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Braunschweig University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6738.a", 
          "name": [
            "Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig 38106, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dammeyer", 
        "givenName": "Thorben", 
        "id": "sg:person.01211513517.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211513517.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Braunschweig University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6738.a", 
          "name": [
            "Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig 38106, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tinnefeld", 
        "givenName": "Philip", 
        "id": "sg:person.01201140546.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01201140546.72"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.1114444108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000052620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja309768d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000501228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001358751", 
          "https://doi.org/10.1038/nmeth.1202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1068539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002422087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-physchem-032210-103340", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004594267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-physchem-032811-112012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004604397"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0810028105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005599452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.201402423", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007246717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0102-87", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007758048", 
          "https://doi.org/10.1038/nbt0102-87"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0102-87", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007758048", 
          "https://doi.org/10.1038/nbt0102-87"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.200802376", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013666545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1124618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014313303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b406492e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014713906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.201402179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016016358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/molecules16043106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021694608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/molecules16043106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021694608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1127344", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022108219"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0053004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024173570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/41048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025621951", 
          "https://doi.org/10.1038/41048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/41048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025621951", 
          "https://doi.org/10.1038/41048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2859-12-49", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026132672", 
          "https://doi.org/10.1186/1475-2859-12-49"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04586", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028635122", 
          "https://doi.org/10.1038/nature04586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04586", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028635122", 
          "https://doi.org/10.1038/nature04586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04586", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028635122", 
          "https://doi.org/10.1038/nature04586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.201000189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031537797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.201000189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031537797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/nl304492y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033025738"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c4cp00127c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033034085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033855926", 
          "https://doi.org/10.1038/nature10016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.104.049452", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034225128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja806590m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034525754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja806590m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034525754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1258", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037551043", 
          "https://doi.org/10.1038/nmeth.1258"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2014.079", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041856810", 
          "https://doi.org/10.1038/nprot.2014.079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2254", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043093081", 
          "https://doi.org/10.1038/nmeth.2254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043491552", 
          "https://doi.org/10.1038/nmeth.1768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth1209-859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045465741", 
          "https://doi.org/10.1038/nmeth1209-859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth1209-859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045465741", 
          "https://doi.org/10.1038/nmeth1209-859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gks326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048891004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja901407j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050955329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja901407j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050955329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gku273", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051259469"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3cs60171d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051969451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1406593111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051980239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi047581f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055198610"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi047581f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055198610"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp991968o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056131166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp991968o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056131166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.273.5280.1392", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062554061"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/oe.21.010978", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1065203711"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-11", 
    "datePublishedReg": "2015-11-01", 
    "description": "Photostability is one of the crucial properties of a fluorophore which strongly influences the quality of single molecule-based super-resolution imaging. Enhanced yellow fluorescent protein (eYFP) is one of the most widely used versions of fluorescent proteins in modern cell biology exhibiting fast intrinsic blinking and reversible photoactivation by UV light. Here, we developed an assay for studying photostabilization of single eYFP molecules with respect to fast blinking and demonstrated a 6-fold enhanced photostability of single eYFP molecules with a beneficial influence on the blinking kinetics under oxygen removal and addition of aliphatic thiols (dSTORM-buffer). Conjugation to single stranded DNA and immobilization via DNA hybridization on a DNA origami 12 helix bundle in aqueous solution allowed photophyiscal studies of eYFP at the single-molecule level and at close to physiological conditions. The benefit of improved photophysical properties for localization-based super-resolution microscopy is demonstrated and quantitatively characterized by imaging 12 helix bundle DNA origami nanorulers with binding sites at designed distances of 160 and 100 nm and by imaging microtubules in fixed mammalian Vero cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep14075", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5871472", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "Super-Resolution Imaging Conditions for enhanced Yellow Fluorescent Protein (eYFP) Demonstrated on DNA Origami Nanorulers", 
    "pagination": "14075", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a85886db36ecf862c5cd464e8a6ec3b4a64e0e349985cd809a8bbbcde1bda280"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26373229"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep14075"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1035340319"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep14075", 
      "https://app.dimensions.ai/details/publication/pub.1035340319"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000425.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/srep14075"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep14075'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep14075'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep14075'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep14075'


 

This table displays all metadata directly associated to this object as RDF triples.

262 TRIPLES      21 PREDICATES      77 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep14075 schema:about N18c588d5cd58430a8b425d173dba78cc
2 N20a40a44fc11490bb1e8153ebc303d1d
3 N57b7c215a38c4cebbdeda9c5c7cdb716
4 N6e7ed1518e254a2a86838145a2d4606c
5 N73291923bb6646e2aae7c9efd24ba968
6 Nad0af63e835b4229a627c758413fdbf2
7 Nd9db2174013f45919d5999b7eafbd6fc
8 Ndbda4348c0ab4139857d34a2633d4657
9 Nf8ab3775dabc4f8282316c00d65ce3ef
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N48911c9a4d484850af05cf99a556dd9e
13 schema:citation sg:pub.10.1038/41048
14 sg:pub.10.1038/nature04586
15 sg:pub.10.1038/nature10016
16 sg:pub.10.1038/nbt0102-87
17 sg:pub.10.1038/nmeth.1202
18 sg:pub.10.1038/nmeth.1258
19 sg:pub.10.1038/nmeth.1768
20 sg:pub.10.1038/nmeth.2254
21 sg:pub.10.1038/nmeth1209-859
22 sg:pub.10.1038/nprot.2014.079
23 sg:pub.10.1186/1475-2859-12-49
24 https://doi.org/10.1002/anie.200802376
25 https://doi.org/10.1002/cphc.201000189
26 https://doi.org/10.1002/cphc.201402179
27 https://doi.org/10.1002/cphc.201402423
28 https://doi.org/10.1021/bi047581f
29 https://doi.org/10.1021/ja309768d
30 https://doi.org/10.1021/ja806590m
31 https://doi.org/10.1021/ja901407j
32 https://doi.org/10.1021/jp991968o
33 https://doi.org/10.1021/nl304492y
34 https://doi.org/10.1039/b406492e
35 https://doi.org/10.1039/c3cs60171d
36 https://doi.org/10.1039/c4cp00127c
37 https://doi.org/10.1073/pnas.0810028105
38 https://doi.org/10.1073/pnas.1114444108
39 https://doi.org/10.1073/pnas.1406593111
40 https://doi.org/10.1093/nar/gks326
41 https://doi.org/10.1093/nar/gku273
42 https://doi.org/10.1126/science.1068539
43 https://doi.org/10.1126/science.1124618
44 https://doi.org/10.1126/science.1127344
45 https://doi.org/10.1126/science.273.5280.1392
46 https://doi.org/10.1146/annurev-physchem-032210-103340
47 https://doi.org/10.1146/annurev-physchem-032811-112012
48 https://doi.org/10.1364/oe.21.010978
49 https://doi.org/10.1371/journal.pone.0053004
50 https://doi.org/10.1529/biophysj.104.049452
51 https://doi.org/10.3390/molecules16043106
52 schema:datePublished 2015-11
53 schema:datePublishedReg 2015-11-01
54 schema:description Photostability is one of the crucial properties of a fluorophore which strongly influences the quality of single molecule-based super-resolution imaging. Enhanced yellow fluorescent protein (eYFP) is one of the most widely used versions of fluorescent proteins in modern cell biology exhibiting fast intrinsic blinking and reversible photoactivation by UV light. Here, we developed an assay for studying photostabilization of single eYFP molecules with respect to fast blinking and demonstrated a 6-fold enhanced photostability of single eYFP molecules with a beneficial influence on the blinking kinetics under oxygen removal and addition of aliphatic thiols (dSTORM-buffer). Conjugation to single stranded DNA and immobilization via DNA hybridization on a DNA origami 12 helix bundle in aqueous solution allowed photophyiscal studies of eYFP at the single-molecule level and at close to physiological conditions. The benefit of improved photophysical properties for localization-based super-resolution microscopy is demonstrated and quantitatively characterized by imaging 12 helix bundle DNA origami nanorulers with binding sites at designed distances of 160 and 100 nm and by imaging microtubules in fixed mammalian Vero cells.
55 schema:genre research_article
56 schema:inLanguage en
57 schema:isAccessibleForFree true
58 schema:isPartOf N78874297e2bb4887a22365a0145709d1
59 N8470a9b6a5e14bf3b09bbd5660fb1eba
60 sg:journal.1045337
61 schema:name Super-Resolution Imaging Conditions for enhanced Yellow Fluorescent Protein (eYFP) Demonstrated on DNA Origami Nanorulers
62 schema:pagination 14075
63 schema:productId N0d2830ac9e2e411189a74dcead9bbe87
64 N15a5daf97d5b406f8f844dca06e33df6
65 N2c0d867d6c8049628ea0d0c30120230d
66 N3b51f0bffec048de8d92dfe79ec640d1
67 Ne60a2c23d4ac407bb1195496c190ebc3
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035340319
69 https://doi.org/10.1038/srep14075
70 schema:sdDatePublished 2019-04-10T18:08
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher Ncb668567b08b46da8dc2b9424940df6f
73 schema:url http://www.nature.com/articles/srep14075
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N0d2830ac9e2e411189a74dcead9bbe87 schema:name dimensions_id
78 schema:value pub.1035340319
79 rdf:type schema:PropertyValue
80 N15a5daf97d5b406f8f844dca06e33df6 schema:name nlm_unique_id
81 schema:value 101563288
82 rdf:type schema:PropertyValue
83 N18c588d5cd58430a8b425d173dba78cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Vero Cells
85 rdf:type schema:DefinedTerm
86 N20a40a44fc11490bb1e8153ebc303d1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Fluorescent Dyes
88 rdf:type schema:DefinedTerm
89 N2c0d867d6c8049628ea0d0c30120230d schema:name pubmed_id
90 schema:value 26373229
91 rdf:type schema:PropertyValue
92 N3486efd008ee4b418e7164f2817bc1f6 rdf:first sg:person.01206452510.07
93 rdf:rest Ncb7cbbf0dfd24e45a51c2edde2ca81a2
94 N3b51f0bffec048de8d92dfe79ec640d1 schema:name readcube_id
95 schema:value a85886db36ecf862c5cd464e8a6ec3b4a64e0e349985cd809a8bbbcde1bda280
96 rdf:type schema:PropertyValue
97 N48911c9a4d484850af05cf99a556dd9e rdf:first sg:person.01072667764.32
98 rdf:rest Nd36f4b713f0c4bf4b23206d99f7cdd36
99 N57b7c215a38c4cebbdeda9c5c7cdb716 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Luminescent Proteins
101 rdf:type schema:DefinedTerm
102 N58815ddc3ffe4fac901b6458c71c69b8 rdf:first sg:person.01201140546.72
103 rdf:rest rdf:nil
104 N6e7ed1518e254a2a86838145a2d4606c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name DNA
106 rdf:type schema:DefinedTerm
107 N73291923bb6646e2aae7c9efd24ba968 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Nanotechnology
109 rdf:type schema:DefinedTerm
110 N78874297e2bb4887a22365a0145709d1 schema:issueNumber 1
111 rdf:type schema:PublicationIssue
112 N8470a9b6a5e14bf3b09bbd5660fb1eba schema:volumeNumber 5
113 rdf:type schema:PublicationVolume
114 Nad0af63e835b4229a627c758413fdbf2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Molecular Imaging
116 rdf:type schema:DefinedTerm
117 Ncb668567b08b46da8dc2b9424940df6f schema:name Springer Nature - SN SciGraph project
118 rdf:type schema:Organization
119 Ncb7cbbf0dfd24e45a51c2edde2ca81a2 rdf:first sg:person.01211513517.92
120 rdf:rest N58815ddc3ffe4fac901b6458c71c69b8
121 Nd36f4b713f0c4bf4b23206d99f7cdd36 rdf:first sg:person.01210231443.08
122 rdf:rest N3486efd008ee4b418e7164f2817bc1f6
123 Nd9db2174013f45919d5999b7eafbd6fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Microscopy, Fluorescence
125 rdf:type schema:DefinedTerm
126 Ndbda4348c0ab4139857d34a2633d4657 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Animals
128 rdf:type schema:DefinedTerm
129 Ne60a2c23d4ac407bb1195496c190ebc3 schema:name doi
130 schema:value 10.1038/srep14075
131 rdf:type schema:PropertyValue
132 Nf8ab3775dabc4f8282316c00d65ce3ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Cercopithecus aethiops
134 rdf:type schema:DefinedTerm
135 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
136 schema:name Biological Sciences
137 rdf:type schema:DefinedTerm
138 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biochemistry and Cell Biology
140 rdf:type schema:DefinedTerm
141 sg:grant.5871472 http://pending.schema.org/fundedItem sg:pub.10.1038/srep14075
142 rdf:type schema:MonetaryGrant
143 sg:journal.1045337 schema:issn 2045-2322
144 schema:name Scientific Reports
145 rdf:type schema:Periodical
146 sg:person.01072667764.32 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
147 schema:familyName Jusuk
148 schema:givenName Ija
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072667764.32
150 rdf:type schema:Person
151 sg:person.01201140546.72 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
152 schema:familyName Tinnefeld
153 schema:givenName Philip
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01201140546.72
155 rdf:type schema:Person
156 sg:person.01206452510.07 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
157 schema:familyName Raab
158 schema:givenName Mario
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206452510.07
160 rdf:type schema:Person
161 sg:person.01210231443.08 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
162 schema:familyName Vietz
163 schema:givenName Carolin
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210231443.08
165 rdf:type schema:Person
166 sg:person.01211513517.92 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
167 schema:familyName Dammeyer
168 schema:givenName Thorben
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211513517.92
170 rdf:type schema:Person
171 sg:pub.10.1038/41048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025621951
172 https://doi.org/10.1038/41048
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/nature04586 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028635122
175 https://doi.org/10.1038/nature04586
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/nature10016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033855926
178 https://doi.org/10.1038/nature10016
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/nbt0102-87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007758048
181 https://doi.org/10.1038/nbt0102-87
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/nmeth.1202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001358751
184 https://doi.org/10.1038/nmeth.1202
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/nmeth.1258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037551043
187 https://doi.org/10.1038/nmeth.1258
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/nmeth.1768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043491552
190 https://doi.org/10.1038/nmeth.1768
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/nmeth.2254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043093081
193 https://doi.org/10.1038/nmeth.2254
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/nmeth1209-859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045465741
196 https://doi.org/10.1038/nmeth1209-859
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/nprot.2014.079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041856810
199 https://doi.org/10.1038/nprot.2014.079
200 rdf:type schema:CreativeWork
201 sg:pub.10.1186/1475-2859-12-49 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026132672
202 https://doi.org/10.1186/1475-2859-12-49
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1002/anie.200802376 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013666545
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1002/cphc.201000189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031537797
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1002/cphc.201402179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016016358
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1002/cphc.201402423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007246717
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1021/bi047581f schema:sameAs https://app.dimensions.ai/details/publication/pub.1055198610
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1021/ja309768d schema:sameAs https://app.dimensions.ai/details/publication/pub.1000501228
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1021/ja806590m schema:sameAs https://app.dimensions.ai/details/publication/pub.1034525754
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1021/ja901407j schema:sameAs https://app.dimensions.ai/details/publication/pub.1050955329
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1021/jp991968o schema:sameAs https://app.dimensions.ai/details/publication/pub.1056131166
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1021/nl304492y schema:sameAs https://app.dimensions.ai/details/publication/pub.1033025738
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1039/b406492e schema:sameAs https://app.dimensions.ai/details/publication/pub.1014713906
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1039/c3cs60171d schema:sameAs https://app.dimensions.ai/details/publication/pub.1051969451
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1039/c4cp00127c schema:sameAs https://app.dimensions.ai/details/publication/pub.1033034085
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1073/pnas.0810028105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005599452
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1073/pnas.1114444108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000052620
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1073/pnas.1406593111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051980239
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1093/nar/gks326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048891004
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1093/nar/gku273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051259469
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1126/science.1068539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002422087
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1126/science.1124618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014313303
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1126/science.1127344 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022108219
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1126/science.273.5280.1392 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062554061
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1146/annurev-physchem-032210-103340 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004594267
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1146/annurev-physchem-032811-112012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004604397
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1364/oe.21.010978 schema:sameAs https://app.dimensions.ai/details/publication/pub.1065203711
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1371/journal.pone.0053004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024173570
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1529/biophysj.104.049452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034225128
257 rdf:type schema:CreativeWork
258 https://doi.org/10.3390/molecules16043106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021694608
259 rdf:type schema:CreativeWork
260 https://www.grid.ac/institutes/grid.6738.a schema:alternateName Braunschweig University of Technology
261 schema:name Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig 38106, Germany
262 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...