An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-11

AUTHORS

Keitaro Yamashita, Dongqing Pan, Tomohiko Okuda, Michihiro Sugahara, Atsushi Kodan, Tomohiro Yamaguchi, Tomohiro Murai, Keiko Gomi, Naoki Kajiyama, Eiichi Mizohata, Mamoru Suzuki, Eriko Nango, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Jaehyun Park, Changyong Song, Takaki Hatsui, Makina Yabashi, So Iwata, Hiroaki Kato, Hideo Ago, Masaki Yamamoto, Toru Nakatsu

ABSTRACT

Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to producing large well diffracting crystals. Efficient de novo phasing methods are highly demanding and as such most SFX structures have been determined by molecular replacement methods. Here we employed single isomorphous replacement with anomalous scattering (SIRAS) for phasing and demonstrate successful application to SFX de novo phasing. Only about 20,000 patterns in total were needed for SIRAS phasing while single wavelength anomalous dispersion (SAD) phasing was unsuccessful with more than 80,000 patterns of derivative crystals. We employed high energy X-rays from SACLA (12.6 keV) to take advantage of the large anomalous enhancement near the LIII absorption edge of Hg, which is one of the most widely used heavy atoms for phasing in conventional protein crystallography. Hard XFEL is of benefit for de novo phasing in the use of routinely used heavy atoms and high resolution data collection. More... »

PAGES

14017

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep14017

DOI

http://dx.doi.org/10.1038/srep14017

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003457620

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26360462


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Other Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crystallography, X-Ray", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamashita", 
        "givenName": "Keitaro", 
        "id": "sg:person.0624540116.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624540116.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pan", 
        "givenName": "Dongqing", 
        "id": "sg:person.01117704710.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117704710.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Okuda", 
        "givenName": "Tomohiko", 
        "id": "sg:person.01260512444.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260512444.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sugahara", 
        "givenName": "Michihiro", 
        "id": "sg:person.01347760530.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347760530.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Institute for Integrated Cell-Material Sciences, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kodan", 
        "givenName": "Atsushi", 
        "id": "sg:person.0634566464.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634566464.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamaguchi", 
        "givenName": "Tomohiro", 
        "id": "sg:person.0703416220.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703416220.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murai", 
        "givenName": "Tomohiro", 
        "id": "sg:person.01232175043.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232175043.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kikkoman (Japan)", 
          "id": "https://www.grid.ac/institutes/grid.419775.9", 
          "name": [
            "Research and Development Division, Kikkoman Corporation, Noda, 278-0037, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gomi", 
        "givenName": "Keiko", 
        "id": "sg:person.0671711022.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671711022.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kikkoman (Japan)", 
          "id": "https://www.grid.ac/institutes/grid.419775.9", 
          "name": [
            "Research and Development Division, Kikkoman Corporation, Noda, 278-0037, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kajiyama", 
        "givenName": "Naoki", 
        "id": "sg:person.01061775467.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061775467.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Osaka University", 
          "id": "https://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mizohata", 
        "givenName": "Eiichi", 
        "id": "sg:person.01245206475.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245206475.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Osaka University", 
          "id": "https://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan.", 
            "Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, 565-0871, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suzuki", 
        "givenName": "Mamoru", 
        "id": "sg:person.010737051264.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010737051264.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nango", 
        "givenName": "Eriko", 
        "id": "sg:person.01300647602.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300647602.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Synchrotron Radiation Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.410592.b", 
          "name": [
            "Japan Synchrotron Radiation Research Institute, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tono", 
        "givenName": "Kensuke", 
        "id": "sg:person.01130611300.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130611300.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Synchrotron Radiation Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.410592.b", 
          "name": [
            "Japan Synchrotron Radiation Research Institute, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Joti", 
        "givenName": "Yasumasa", 
        "id": "sg:person.0700134300.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700134300.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Synchrotron Radiation Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.410592.b", 
          "name": [
            "Japan Synchrotron Radiation Research Institute, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kameshima", 
        "givenName": "Takashi", 
        "id": "sg:person.01011504731.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011504731.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Park", 
        "givenName": "Jaehyun", 
        "id": "sg:person.0676614274.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676614274.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "Changyong", 
        "id": "sg:person.01075363531.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01075363531.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hatsui", 
        "givenName": "Takaki", 
        "id": "sg:person.01212263613.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212263613.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yabashi", 
        "givenName": "Makina", 
        "id": "sg:person.0653422474.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653422474.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan.", 
            "Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Iwata", 
        "givenName": "So", 
        "id": "sg:person.01337410152.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337410152.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan.", 
            "Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kato", 
        "givenName": "Hiroaki", 
        "id": "sg:person.014347406071.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014347406071.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ago", 
        "givenName": "Hideo", 
        "id": "sg:person.0662436426.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662436426.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamoto", 
        "givenName": "Masaki", 
        "id": "sg:person.012771421304.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012771421304.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "RIKEN SPring-8 Center, Sayo, 679-5148, Japan.", 
            "Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakatsu", 
        "givenName": "Toru", 
        "id": "sg:person.012462210505.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012462210505.49"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1107/s0907444910007493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444910007493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889891010634", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001689305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2008.91", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003221462", 
          "https://doi.org/10.1038/nprot.2008.91"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09750", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004646089", 
          "https://doi.org/10.1038/nature09750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444912001308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005539810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889812002312", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005571973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909038360", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007316761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909038360", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007316761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m105528200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008864744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444905019670", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008907507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2887", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009464100", 
          "https://doi.org/10.1038/nmeth.2887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature13453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010816082", 
          "https://doi.org/10.1038/nature13453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb0497-269", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013702320", 
          "https://doi.org/10.1038/nsb0497-269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1218231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014005434"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.05421", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018147401"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909042073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018458004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909042073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018458004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909052925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020777046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909052925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020777046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889804010660", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021108776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889801017824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021741422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1259357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023397509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0108767310050981", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024948274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature12773", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025135110", 
          "https://doi.org/10.1038/nature12773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889891010385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025677107"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s1600577514028203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025820361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nphoton.2012.141", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028779905", 
          "https://doi.org/10.1038/nphoton.2012.141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909047337", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032162623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909047337", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032162623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444911001314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032555428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/28.1.235", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035055456"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.3172", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036283182", 
          "https://doi.org/10.1038/nmeth.3172"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4020-6316-9_4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039217561", 
          "https://doi.org/10.1007/978-1-4020-6316-9_4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/oe.18.005713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041299908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/oe.18.005713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041299908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.4867668", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047395776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.107.218102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049569490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.107.218102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049569490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0567739476001873", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051862643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s1399004714013534", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052873598"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0909049508040430", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053175494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1229663", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062467150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/oe.19.002866", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1065195816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/oe.19.002866", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1065195816"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-11", 
    "datePublishedReg": "2015-11-01", 
    "description": "Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to producing large well diffracting crystals. Efficient de novo phasing methods are highly demanding and as such most SFX structures have been determined by molecular replacement methods. Here we employed single isomorphous replacement with anomalous scattering (SIRAS) for phasing and demonstrate successful application to SFX de novo phasing. Only about 20,000 patterns in total were needed for SIRAS phasing while single wavelength anomalous dispersion (SAD) phasing was unsuccessful with more than 80,000 patterns of derivative crystals. We employed high energy X-rays from SACLA (12.6\u2009keV) to take advantage of the large anomalous enhancement near the LIII absorption edge of Hg, which is one of the most widely used heavy atoms for phasing in conventional protein crystallography. Hard XFEL is of benefit for de novo phasing in the use of routinely used heavy atoms and high resolution data collection. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep14017", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5870160", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6139175", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography", 
    "pagination": "14017", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1078676196eacd22c0ed6a8a15ef36e9cc142cbf5945be697c444a26db34c72e"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26360462"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep14017"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1003457620"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep14017", 
      "https://app.dimensions.ai/details/publication/pub.1003457620"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:28", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000421.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/srep/2015/150911/srep14017/full/srep14017.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep14017'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep14017'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep14017'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep14017'


 

This table displays all metadata directly associated to this object as RDF triples.

397 TRIPLES      21 PREDICATES      69 URIs      24 LITERALS      12 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep14017 schema:about N0fb4d9a676f740c993ed3bf94d1cc0d6
2 N2342f5020e5a4fe99bfd6ce258a88056
3 N9dc96b0c9bc34952b7c35b9ce30a5db9
4 anzsrc-for:02
5 anzsrc-for:0299
6 schema:author N9ad68cd37f5c4f92a505a3384795fdd0
7 schema:citation sg:pub.10.1007/978-1-4020-6316-9_4
8 sg:pub.10.1038/nature09750
9 sg:pub.10.1038/nature12773
10 sg:pub.10.1038/nature13453
11 sg:pub.10.1038/nmeth.2887
12 sg:pub.10.1038/nmeth.3172
13 sg:pub.10.1038/nphoton.2012.141
14 sg:pub.10.1038/nprot.2008.91
15 sg:pub.10.1038/nsb0497-269
16 https://doi.org/10.1063/1.4867668
17 https://doi.org/10.1074/jbc.m105528200
18 https://doi.org/10.1093/nar/28.1.235
19 https://doi.org/10.1103/physrevlett.107.218102
20 https://doi.org/10.1107/s0021889801017824
21 https://doi.org/10.1107/s0021889804010660
22 https://doi.org/10.1107/s0021889812002312
23 https://doi.org/10.1107/s0021889891010385
24 https://doi.org/10.1107/s0021889891010634
25 https://doi.org/10.1107/s0108767310050981
26 https://doi.org/10.1107/s0567739476001873
27 https://doi.org/10.1107/s0907444905019670
28 https://doi.org/10.1107/s0907444909038360
29 https://doi.org/10.1107/s0907444909042073
30 https://doi.org/10.1107/s0907444909047337
31 https://doi.org/10.1107/s0907444909052925
32 https://doi.org/10.1107/s0907444910007493
33 https://doi.org/10.1107/s0907444911001314
34 https://doi.org/10.1107/s0907444912001308
35 https://doi.org/10.1107/s0909049508040430
36 https://doi.org/10.1107/s1399004714013534
37 https://doi.org/10.1107/s1600577514028203
38 https://doi.org/10.1126/science.1218231
39 https://doi.org/10.1126/science.1229663
40 https://doi.org/10.1126/science.1259357
41 https://doi.org/10.1364/oe.18.005713
42 https://doi.org/10.1364/oe.19.002866
43 https://doi.org/10.7554/elife.05421
44 schema:datePublished 2015-11
45 schema:datePublishedReg 2015-11-01
46 schema:description Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to producing large well diffracting crystals. Efficient de novo phasing methods are highly demanding and as such most SFX structures have been determined by molecular replacement methods. Here we employed single isomorphous replacement with anomalous scattering (SIRAS) for phasing and demonstrate successful application to SFX de novo phasing. Only about 20,000 patterns in total were needed for SIRAS phasing while single wavelength anomalous dispersion (SAD) phasing was unsuccessful with more than 80,000 patterns of derivative crystals. We employed high energy X-rays from SACLA (12.6 keV) to take advantage of the large anomalous enhancement near the LIII absorption edge of Hg, which is one of the most widely used heavy atoms for phasing in conventional protein crystallography. Hard XFEL is of benefit for de novo phasing in the use of routinely used heavy atoms and high resolution data collection.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree true
50 schema:isPartOf N6358424f1ad04a40a6edd505813148f0
51 Na688d0db073b424d95266dafb4767192
52 sg:journal.1045337
53 schema:name An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography
54 schema:pagination 14017
55 schema:productId N07ec16275ac1495890aee81283006b93
56 N624023f9412a4e4ea02a6b466ff43fff
57 N8e86ebfba27f4562bbe1c65ac5a6bdc8
58 Nb96bde434fd4421d820b621ca6a06d98
59 Nddb58d4f577a452dbb02ab319e8c31bb
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003457620
61 https://doi.org/10.1038/srep14017
62 schema:sdDatePublished 2019-04-10T16:28
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher N409668fe6bbb4c608bdc7cf2bd60c804
65 schema:url http://www.nature.com/srep/2015/150911/srep14017/full/srep14017.html
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N03083c09a6164b3da371717e41d4eac3 rdf:first sg:person.01245206475.10
70 rdf:rest N8755acc54485488fa17452bb5fd9416b
71 N06f9651d05f441fbadcff8b3f0dea4ec rdf:first sg:person.01011504731.06
72 rdf:rest Nf92a6d2a54494b3c90e63cc426b17715
73 N07aeba92baef45bc86849226a8b3cd21 rdf:first sg:person.0700134300.34
74 rdf:rest N06f9651d05f441fbadcff8b3f0dea4ec
75 N07ec16275ac1495890aee81283006b93 schema:name doi
76 schema:value 10.1038/srep14017
77 rdf:type schema:PropertyValue
78 N0cb03022d803448385f455b8c084f995 schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
79 rdf:type schema:Organization
80 N0fb4d9a676f740c993ed3bf94d1cc0d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Proteins
82 rdf:type schema:DefinedTerm
83 N1e2e1865eba14a328423fac395632001 rdf:first sg:person.01300647602.69
84 rdf:rest Nfe86019689dc4991b26ecc9f2816d806
85 N224fa5a9837248d681ea9a28141ee7a7 rdf:first sg:person.01232175043.55
86 rdf:rest N4540ae3924864bb097aa90c3e485f88c
87 N2342f5020e5a4fe99bfd6ce258a88056 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Crystallography, X-Ray
89 rdf:type schema:DefinedTerm
90 N243e0ffc5cbd458ea262bab0d99c2c7b schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
91 rdf:type schema:Organization
92 N409668fe6bbb4c608bdc7cf2bd60c804 schema:name Springer Nature - SN SciGraph project
93 rdf:type schema:Organization
94 N4540ae3924864bb097aa90c3e485f88c rdf:first sg:person.0671711022.62
95 rdf:rest N5e96c6f6af354219a3c8b0c10fbac9bb
96 N49db904a1f6c4cb4bce842be67222098 rdf:first sg:person.0634566464.14
97 rdf:rest Nbd05247a9bca49f098dd741fa7cb6d18
98 N4e9647cd4fd84e13a239b5cccbec0cc5 schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
99 rdf:type schema:Organization
100 N5e96c6f6af354219a3c8b0c10fbac9bb rdf:first sg:person.01061775467.16
101 rdf:rest N03083c09a6164b3da371717e41d4eac3
102 N6073876822ac4e19952bbd195467dfd4 schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
103 rdf:type schema:Organization
104 N624023f9412a4e4ea02a6b466ff43fff schema:name dimensions_id
105 schema:value pub.1003457620
106 rdf:type schema:PropertyValue
107 N6358424f1ad04a40a6edd505813148f0 schema:issueNumber 1
108 rdf:type schema:PublicationIssue
109 N63c455b1a8c84e1d8f25d9f371efd022 rdf:first sg:person.01075363531.07
110 rdf:rest Nde3d7c415dcb4b53a1bafb32994eb66f
111 N7536ccbda11744c8ada955efbb2b4b8d rdf:first sg:person.0653422474.51
112 rdf:rest Nfa0d95c76f174aef8cfe896ded32a100
113 N76bf02e2d696459c88a4c481dbab18f4 schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
114 rdf:type schema:Organization
115 N7ac3e9f63dcf4ba0a5a4ff48a7b14347 rdf:first sg:person.01260512444.18
116 rdf:rest Nd02bc13ef61a4745b466d24acea1c843
117 N8755acc54485488fa17452bb5fd9416b rdf:first sg:person.010737051264.51
118 rdf:rest N1e2e1865eba14a328423fac395632001
119 N8e4cc52b295b48d28de8729a33af74a5 rdf:first sg:person.012771421304.81
120 rdf:rest Nd7ad3e39d2954f719d16c028688a487d
121 N8e86ebfba27f4562bbe1c65ac5a6bdc8 schema:name readcube_id
122 schema:value 1078676196eacd22c0ed6a8a15ef36e9cc142cbf5945be697c444a26db34c72e
123 rdf:type schema:PropertyValue
124 N9450cb4cfa88438092c01371a4b55df3 rdf:first sg:person.01117704710.17
125 rdf:rest N7ac3e9f63dcf4ba0a5a4ff48a7b14347
126 N9ad68cd37f5c4f92a505a3384795fdd0 rdf:first sg:person.0624540116.94
127 rdf:rest N9450cb4cfa88438092c01371a4b55df3
128 N9dc96b0c9bc34952b7c35b9ce30a5db9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Models, Molecular
130 rdf:type schema:DefinedTerm
131 N9e79f263a2184f62b32719ee3284b01a rdf:first sg:person.014347406071.15
132 rdf:rest Nfa5d315b330742d39e95b08abc0c1d93
133 Na688d0db073b424d95266dafb4767192 schema:volumeNumber 5
134 rdf:type schema:PublicationVolume
135 Na9971be986844f40bafcb404245677b9 schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
136 rdf:type schema:Organization
137 Nb96bde434fd4421d820b621ca6a06d98 schema:name pubmed_id
138 schema:value 26360462
139 rdf:type schema:PropertyValue
140 Nbd05247a9bca49f098dd741fa7cb6d18 rdf:first sg:person.0703416220.41
141 rdf:rest N224fa5a9837248d681ea9a28141ee7a7
142 Nc68a81e14d3f4ff7b8f81cfb65a6250e schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
143 rdf:type schema:Organization
144 Ncc1a56c24d664ef1a474ec99f797c09c schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
145 rdf:type schema:Organization
146 Nd02bc13ef61a4745b466d24acea1c843 rdf:first sg:person.01347760530.91
147 rdf:rest N49db904a1f6c4cb4bce842be67222098
148 Nd7ad3e39d2954f719d16c028688a487d rdf:first sg:person.012462210505.49
149 rdf:rest rdf:nil
150 Nddb58d4f577a452dbb02ab319e8c31bb schema:name nlm_unique_id
151 schema:value 101563288
152 rdf:type schema:PropertyValue
153 Nde3d7c415dcb4b53a1bafb32994eb66f rdf:first sg:person.01212263613.30
154 rdf:rest N7536ccbda11744c8ada955efbb2b4b8d
155 Nec6c28c160ab431da557f528bb0a4bcd schema:name RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
156 rdf:type schema:Organization
157 Nf92a6d2a54494b3c90e63cc426b17715 rdf:first sg:person.0676614274.02
158 rdf:rest N63c455b1a8c84e1d8f25d9f371efd022
159 Nfa0d95c76f174aef8cfe896ded32a100 rdf:first sg:person.01337410152.19
160 rdf:rest N9e79f263a2184f62b32719ee3284b01a
161 Nfa5d315b330742d39e95b08abc0c1d93 rdf:first sg:person.0662436426.00
162 rdf:rest N8e4cc52b295b48d28de8729a33af74a5
163 Nfe86019689dc4991b26ecc9f2816d806 rdf:first sg:person.01130611300.43
164 rdf:rest N07aeba92baef45bc86849226a8b3cd21
165 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
166 schema:name Physical Sciences
167 rdf:type schema:DefinedTerm
168 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
169 schema:name Other Physical Sciences
170 rdf:type schema:DefinedTerm
171 sg:grant.5870160 http://pending.schema.org/fundedItem sg:pub.10.1038/srep14017
172 rdf:type schema:MonetaryGrant
173 sg:grant.6139175 http://pending.schema.org/fundedItem sg:pub.10.1038/srep14017
174 rdf:type schema:MonetaryGrant
175 sg:journal.1045337 schema:issn 2045-2322
176 schema:name Scientific Reports
177 rdf:type schema:Periodical
178 sg:person.01011504731.06 schema:affiliation https://www.grid.ac/institutes/grid.410592.b
179 schema:familyName Kameshima
180 schema:givenName Takashi
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011504731.06
182 rdf:type schema:Person
183 sg:person.01061775467.16 schema:affiliation https://www.grid.ac/institutes/grid.419775.9
184 schema:familyName Kajiyama
185 schema:givenName Naoki
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061775467.16
187 rdf:type schema:Person
188 sg:person.010737051264.51 schema:affiliation https://www.grid.ac/institutes/grid.136593.b
189 schema:familyName Suzuki
190 schema:givenName Mamoru
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010737051264.51
192 rdf:type schema:Person
193 sg:person.01075363531.07 schema:affiliation N76bf02e2d696459c88a4c481dbab18f4
194 schema:familyName Song
195 schema:givenName Changyong
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01075363531.07
197 rdf:type schema:Person
198 sg:person.01117704710.17 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
199 schema:familyName Pan
200 schema:givenName Dongqing
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117704710.17
202 rdf:type schema:Person
203 sg:person.01130611300.43 schema:affiliation https://www.grid.ac/institutes/grid.410592.b
204 schema:familyName Tono
205 schema:givenName Kensuke
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130611300.43
207 rdf:type schema:Person
208 sg:person.01212263613.30 schema:affiliation N4e9647cd4fd84e13a239b5cccbec0cc5
209 schema:familyName Hatsui
210 schema:givenName Takaki
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212263613.30
212 rdf:type schema:Person
213 sg:person.01232175043.55 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
214 schema:familyName Murai
215 schema:givenName Tomohiro
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232175043.55
217 rdf:type schema:Person
218 sg:person.01245206475.10 schema:affiliation https://www.grid.ac/institutes/grid.136593.b
219 schema:familyName Mizohata
220 schema:givenName Eiichi
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245206475.10
222 rdf:type schema:Person
223 sg:person.012462210505.49 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
224 schema:familyName Nakatsu
225 schema:givenName Toru
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012462210505.49
227 rdf:type schema:Person
228 sg:person.01260512444.18 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
229 schema:familyName Okuda
230 schema:givenName Tomohiko
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260512444.18
232 rdf:type schema:Person
233 sg:person.012771421304.81 schema:affiliation N6073876822ac4e19952bbd195467dfd4
234 schema:familyName Yamamoto
235 schema:givenName Masaki
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012771421304.81
237 rdf:type schema:Person
238 sg:person.01300647602.69 schema:affiliation Nec6c28c160ab431da557f528bb0a4bcd
239 schema:familyName Nango
240 schema:givenName Eriko
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300647602.69
242 rdf:type schema:Person
243 sg:person.01337410152.19 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
244 schema:familyName Iwata
245 schema:givenName So
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337410152.19
247 rdf:type schema:Person
248 sg:person.01347760530.91 schema:affiliation Ncc1a56c24d664ef1a474ec99f797c09c
249 schema:familyName Sugahara
250 schema:givenName Michihiro
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347760530.91
252 rdf:type schema:Person
253 sg:person.014347406071.15 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
254 schema:familyName Kato
255 schema:givenName Hiroaki
256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014347406071.15
257 rdf:type schema:Person
258 sg:person.0624540116.94 schema:affiliation N243e0ffc5cbd458ea262bab0d99c2c7b
259 schema:familyName Yamashita
260 schema:givenName Keitaro
261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624540116.94
262 rdf:type schema:Person
263 sg:person.0634566464.14 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
264 schema:familyName Kodan
265 schema:givenName Atsushi
266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634566464.14
267 rdf:type schema:Person
268 sg:person.0653422474.51 schema:affiliation Nc68a81e14d3f4ff7b8f81cfb65a6250e
269 schema:familyName Yabashi
270 schema:givenName Makina
271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653422474.51
272 rdf:type schema:Person
273 sg:person.0662436426.00 schema:affiliation Na9971be986844f40bafcb404245677b9
274 schema:familyName Ago
275 schema:givenName Hideo
276 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662436426.00
277 rdf:type schema:Person
278 sg:person.0671711022.62 schema:affiliation https://www.grid.ac/institutes/grid.419775.9
279 schema:familyName Gomi
280 schema:givenName Keiko
281 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671711022.62
282 rdf:type schema:Person
283 sg:person.0676614274.02 schema:affiliation N0cb03022d803448385f455b8c084f995
284 schema:familyName Park
285 schema:givenName Jaehyun
286 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676614274.02
287 rdf:type schema:Person
288 sg:person.0700134300.34 schema:affiliation https://www.grid.ac/institutes/grid.410592.b
289 schema:familyName Joti
290 schema:givenName Yasumasa
291 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700134300.34
292 rdf:type schema:Person
293 sg:person.0703416220.41 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
294 schema:familyName Yamaguchi
295 schema:givenName Tomohiro
296 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703416220.41
297 rdf:type schema:Person
298 sg:pub.10.1007/978-1-4020-6316-9_4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039217561
299 https://doi.org/10.1007/978-1-4020-6316-9_4
300 rdf:type schema:CreativeWork
301 sg:pub.10.1038/nature09750 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004646089
302 https://doi.org/10.1038/nature09750
303 rdf:type schema:CreativeWork
304 sg:pub.10.1038/nature12773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025135110
305 https://doi.org/10.1038/nature12773
306 rdf:type schema:CreativeWork
307 sg:pub.10.1038/nature13453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010816082
308 https://doi.org/10.1038/nature13453
309 rdf:type schema:CreativeWork
310 sg:pub.10.1038/nmeth.2887 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009464100
311 https://doi.org/10.1038/nmeth.2887
312 rdf:type schema:CreativeWork
313 sg:pub.10.1038/nmeth.3172 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036283182
314 https://doi.org/10.1038/nmeth.3172
315 rdf:type schema:CreativeWork
316 sg:pub.10.1038/nphoton.2012.141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028779905
317 https://doi.org/10.1038/nphoton.2012.141
318 rdf:type schema:CreativeWork
319 sg:pub.10.1038/nprot.2008.91 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003221462
320 https://doi.org/10.1038/nprot.2008.91
321 rdf:type schema:CreativeWork
322 sg:pub.10.1038/nsb0497-269 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013702320
323 https://doi.org/10.1038/nsb0497-269
324 rdf:type schema:CreativeWork
325 https://doi.org/10.1063/1.4867668 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047395776
326 rdf:type schema:CreativeWork
327 https://doi.org/10.1074/jbc.m105528200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008864744
328 rdf:type schema:CreativeWork
329 https://doi.org/10.1093/nar/28.1.235 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035055456
330 rdf:type schema:CreativeWork
331 https://doi.org/10.1103/physrevlett.107.218102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049569490
332 rdf:type schema:CreativeWork
333 https://doi.org/10.1107/s0021889801017824 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021741422
334 rdf:type schema:CreativeWork
335 https://doi.org/10.1107/s0021889804010660 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021108776
336 rdf:type schema:CreativeWork
337 https://doi.org/10.1107/s0021889812002312 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005571973
338 rdf:type schema:CreativeWork
339 https://doi.org/10.1107/s0021889891010385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025677107
340 rdf:type schema:CreativeWork
341 https://doi.org/10.1107/s0021889891010634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001689305
342 rdf:type schema:CreativeWork
343 https://doi.org/10.1107/s0108767310050981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024948274
344 rdf:type schema:CreativeWork
345 https://doi.org/10.1107/s0567739476001873 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051862643
346 rdf:type schema:CreativeWork
347 https://doi.org/10.1107/s0907444905019670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008907507
348 rdf:type schema:CreativeWork
349 https://doi.org/10.1107/s0907444909038360 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007316761
350 rdf:type schema:CreativeWork
351 https://doi.org/10.1107/s0907444909042073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018458004
352 rdf:type schema:CreativeWork
353 https://doi.org/10.1107/s0907444909047337 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032162623
354 rdf:type schema:CreativeWork
355 https://doi.org/10.1107/s0907444909052925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020777046
356 rdf:type schema:CreativeWork
357 https://doi.org/10.1107/s0907444910007493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235100
358 rdf:type schema:CreativeWork
359 https://doi.org/10.1107/s0907444911001314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032555428
360 rdf:type schema:CreativeWork
361 https://doi.org/10.1107/s0907444912001308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005539810
362 rdf:type schema:CreativeWork
363 https://doi.org/10.1107/s0909049508040430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053175494
364 rdf:type schema:CreativeWork
365 https://doi.org/10.1107/s1399004714013534 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052873598
366 rdf:type schema:CreativeWork
367 https://doi.org/10.1107/s1600577514028203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025820361
368 rdf:type schema:CreativeWork
369 https://doi.org/10.1126/science.1218231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014005434
370 rdf:type schema:CreativeWork
371 https://doi.org/10.1126/science.1229663 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062467150
372 rdf:type schema:CreativeWork
373 https://doi.org/10.1126/science.1259357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023397509
374 rdf:type schema:CreativeWork
375 https://doi.org/10.1364/oe.18.005713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041299908
376 rdf:type schema:CreativeWork
377 https://doi.org/10.1364/oe.19.002866 schema:sameAs https://app.dimensions.ai/details/publication/pub.1065195816
378 rdf:type schema:CreativeWork
379 https://doi.org/10.7554/elife.05421 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018147401
380 rdf:type schema:CreativeWork
381 https://www.grid.ac/institutes/grid.136593.b schema:alternateName Osaka University
382 schema:name Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan.
383 RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
384 Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, 565-0871, Japan.
385 rdf:type schema:Organization
386 https://www.grid.ac/institutes/grid.258799.8 schema:alternateName Kyoto University
387 schema:name Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, 606-8501, Japan.
388 Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, 606-8501, Japan.
389 Institute for Integrated Cell-Material Sciences, Kyoto University, Sakyo, 606-8501, Japan.
390 RIKEN SPring-8 Center, Sayo, 679-5148, Japan.
391 rdf:type schema:Organization
392 https://www.grid.ac/institutes/grid.410592.b schema:alternateName Japan Synchrotron Radiation Research Institute
393 schema:name Japan Synchrotron Radiation Research Institute, Sayo, 679-5148, Japan.
394 rdf:type schema:Organization
395 https://www.grid.ac/institutes/grid.419775.9 schema:alternateName Kikkoman (Japan)
396 schema:name Research and Development Division, Kikkoman Corporation, Noda, 278-0037, Japan.
397 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...