Improving access to endogenous DNA in ancient bones and teeth View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-09

AUTHORS

Peter B. Damgaard, Ashot Margaryan, Hannes Schroeder, Ludovic Orlando, Eske Willerslev, Morten E. Allentoft

ABSTRACT

Poor DNA preservation is the most limiting factor in ancient genomic research. In the majority of ancient bones and teeth, endogenous DNA molecules represent a minor fraction of the whole DNA extract, rendering shot-gun sequencing inefficient for obtaining genomic data. Based on ancient human bone samples from temperate and tropical environments, we show that an EDTA-based enzymatic 'pre-digestion' of powdered bone increases the proportion of endogenous DNA several fold. By performing the pre-digestion step between 30 min and 6 hours on five bones, we observe an asymptotic increase in endogenous DNA content, with a 2.7-fold average increase reached at 1 hour. We repeat the experiment using a brief pre-digestion (15 or 30 mins) on 21 ancient bones and teeth from a variety of archaeological contexts and observe an improvement in 16 of these. We here advocate the implementation of a brief pre-digestion step as a standard procedure in ancient DNA extractions. Finally, we demonstrate on 14 ancient teeth that by targeting the outer layer of the roots we obtain up to 14 times more endogenous DNA than when using the inner dentine. Our presented methods are likely to increase the proportion of ancient samples that are suitable for genome-scale characterization. More... »

PAGES

11184

References to SciGraph publications

  • 2010-02-11. Ancient human genome sequence of an extinct Palaeo-Eskimo in NATURE
  • 2008. Microbial bioerosion of bone – a review in CURRENT DEVELOPMENTS IN BIOEROSION
  • 2014-01. Upper Palaeolithic Siberian genome reveals dual ancestry of Native Americans in NATURE
  • 2014-01-26. Derived immune and ancestral pigmentation alleles in a 7,000-year-old Mesolithic European in NATURE
  • 2014-09. Ancient human genomes suggest three ancestral populations for present-day Europeans in NATURE
  • 2013-04. Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA in NATURE PROTOCOLS
  • 2014-12. Genome flux and stasis in a five millennium transect of European prehistory in NATURE COMMUNICATIONS
  • 2010-12. Genetic history of an archaic hominin group from Denisova Cave in Siberia in NATURE
  • 2013-12. Targeted sampling of cementum for recovery of nuclear DNA from human teeth and the impact of common decontamination measures in INVESTIGATIVE GENETICS
  • 2010-04. The complete mitochondrial DNA genome of an unknown hominin from southern Siberia in NATURE
  • 2014-02. The genome of a Late Pleistocene human from a Clovis burial site in western Montana in NATURE
  • 2012-12. Improving the performance of true single molecule sequencing for ancient DNA in BMC GENOMICS
  • 2006-12. Quantification of damage in DNA recovered from highly degraded samples – a case study on DNA in faeces in FRONTIERS IN ZOOLOGY
  • 2012-12. AdapterRemoval: easy cleaning of next-generation sequencing reads in BMC RESEARCH NOTES
  • 2012-12. Ancient DNA studies: new perspectives on old samples in GENETICS SELECTION EVOLUTION
  • 2012-12. Improving ancient DNA read mapping against modern reference genomes in BMC GENOMICS
  • 2014-10. Genome sequence of a 45,000-year-old modern human from western Siberia in NATURE
  • 2014-01. The complete genome sequence of a Neanderthal from the Altai Mountains in NATURE
  • 2012-01. New insights into the Tyrolean Iceman's origin and phenotype as inferred by whole-genome sequencing in NATURE COMMUNICATIONS
  • 2013-07. Recalibrating Equus evolution using the genome sequence of an early Middle Pleistocene horse in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep11184

    DOI

    http://dx.doi.org/10.1038/srep11184

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015447928

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26081994


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