A cost-effective RNA sequencing protocol for large-scale gene expression studies View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-08

AUTHORS

Zhonggang Hou, Peng Jiang, Scott A. Swanson, Angela L. Elwell, Bao Kim S. Nguyen, Jennifer M. Bolin, Ron Stewart, James A. Thomson

ABSTRACT

RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. Extensive validation using human embryonic stem cells showed high consistency between technical replicates at various multiplexing levels. More... »

PAGES

9570

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep09570

DOI

http://dx.doi.org/10.1038/srep09570

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005291411

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25831155


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