Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-08

AUTHORS

A T Y Yeung, C Hale, J Xia, P H Tate, D Goulding, J A Keane, S Mukhopadhyay, L Forrester, O Billker, W C Skarnes, R E W Hancock, G Dougan

ABSTRACT

The ability to differentiate genetically modified mouse embryonic stem (ES) cells into functional macrophages provides a potentially attractive resource to study host-pathogen interactions without the need for animal experimentation. This is particularly useful in instances where the gene of interest is essential and a knockout mouse is not available. Here we differentiated mouse ES cells into macrophages in vitro and showed, through a combination of flow cytometry, microscopic imaging, and RNA-Seq, that ES cell-derived macrophages responded to S. Typhimurium, in a comparable manner to mouse bone marrow derived macrophages. We constructed a homozygous mutant mouse ES cell line in the Traf2 gene that is known to play a role in tumour necrosis factor-α signalling but has not been studied for its role in infections or response to Toll-like receptor agonists. Interestingly, traf2-deficient macrophages produced reduced levels of inflammatory cytokines in response to lipopolysaccharide (LPS) or flagellin stimulation and exhibited increased susceptibility to S. Typhimurium infection. More... »

PAGES

8908

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep08908

DOI

http://dx.doi.org/10.1038/srep08908

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029753660

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25752829


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curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep08908'

RDF/XML is a standard XML format for linked data.

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