An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-05

AUTHORS

Xiaoxiao Zhu, Yajie Xu, Shanshan Yu, Lu Lu, Mingqin Ding, Jing Cheng, Guoxu Song, Xing Gao, Liangming Yao, Dongdong Fan, Shu Meng, Xuewen Zhang, Shengdi Hu, Yong Tian

ABSTRACT

The rapid generation of various species and strains of laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, the dominant approach for genotyping of genome-modified animals has been the T7E1 endonuclease cleavage assay. Here, we present a polyacrylamide gel electrophoresis-based (PAGE) method to genotype mice harboring different types of indel mutations. We developed 6 strains of genome-modified mice using CRISPR/Cas9 system, and utilized this approach to genotype mice from F0 to F2 generation, which included single and multiplexed genome-modified mice. We also determined the maximal detection sensitivity for detecting mosaic DNA using PAGE-based assay as 0.5%. We further applied PAGE-based genotyping approach to detect CRISPR/Cas9-mediated on- and off-target effect in human 293T and induced pluripotent stem cells (iPSCs). Thus, PAGE-based genotyping approach meets the rapidly increasing demand for genotyping of the fast-growing number of genome-modified animals and human cell lines created using CRISPR/Cas9 system or other nuclease systems such as TALEN or ZFN. More... »

PAGES

6420

References to SciGraph publications

  • 2013-03. A library of TAL effector nucleases spanning the human genome in NATURE BIOTECHNOLOGY
  • 2014-03. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9 in NATURE
  • 2013-08. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems in NATURE BIOTECHNOLOGY
  • 2013-08. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system in NATURE BIOTECHNOLOGY
  • 2013-09. DNA targeting specificity of RNA-guided Cas9 nucleases in NATURE BIOTECHNOLOGY
  • 2013-09. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells in NATURE BIOTECHNOLOGY
  • 2014-07. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells in NATURE BIOTECHNOLOGY
  • 2014-03. One-step generation of knockout pigs by zygote injection of CRISPR/Cas system in CELL RESEARCH
  • 2013-03. Efficient genome editing in zebrafish using a CRISPR-Cas system in NATURE BIOTECHNOLOGY
  • 2013-01. Knockout mice created by TALEN-mediated gene targeting in NATURE BIOTECHNOLOGY
  • 2013-10. Cas9 as a versatile tool for engineering biology in NATURE METHODS
  • 2011-02. A TALE nuclease architecture for efficient genome editing in NATURE BIOTECHNOLOGY
  • 2007-07. An improved zinc-finger nuclease architecture for highly specific genome editing in NATURE BIOTECHNOLOGY
  • 2014-01. Generating rats with conditional alleles using CRISPR/Cas9 in CELL RESEARCH
  • 2014-12. Genotyping with CRISPR-Cas-derived RNA-guided endonucleases in NATURE COMMUNICATIONS
  • 2013-11. Heritable gene-targeting with gRNA/Cas9 in rats in CELL RESEARCH
  • 2013-05. Generation of gene-modified mice via Cas9/RNA-mediated gene targeting in CELL RESEARCH
  • 2013-04. Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos in CELL RESEARCH
  • 2014-05. High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep06420

    DOI

    http://dx.doi.org/10.1038/srep06420

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010196515

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25236476


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CRISPR-Cas Systems", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genotyping Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "INDEL Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China", 
                "University of Chinese Academy of Sciences, Beijing 100080, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhu", 
            "givenName": "Xiaoxiao", 
            "id": "sg:person.01253311624.80", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253311624.80"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Yajie", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yu", 
            "givenName": "Shanshan", 
            "id": "sg:person.01367540224.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367540224.07"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China", 
                "University of Chinese Academy of Sciences, Beijing 100080, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lu", 
            "givenName": "Lu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China", 
                "University of Chinese Academy of Sciences, Beijing 100080, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ding", 
            "givenName": "Mingqin", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cheng", 
            "givenName": "Jing", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China", 
                "University of Chinese Academy of Sciences, Beijing 100080, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Song", 
            "givenName": "Guoxu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gao", 
            "givenName": "Xing", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yao", 
            "givenName": "Liangming", 
            "id": "sg:person.01344216376.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01344216376.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China", 
                "University of Chinese Academy of Sciences, Beijing 100080, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fan", 
            "givenName": "Dongdong", 
            "id": "sg:person.01143227564.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143227564.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Meng", 
            "givenName": "Shu", 
            "id": "sg:person.01211342764.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211342764.54"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Xuewen", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hu", 
            "givenName": "Shengdi", 
            "id": "sg:person.01321340020.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321340020.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tian", 
            "givenName": "Yong", 
            "id": "sg:person.01132120163.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132120163.89"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nbt1319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002154526", 
              "https://doi.org/10.1038/nbt1319"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1400236111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002427034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbadis.2014.04.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006504293"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.45", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006654084", 
              "https://doi.org/10.1038/cr.2013.45"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.45", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006654084", 
              "https://doi.org/10.1038/cr.2013.45"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1247005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007966801"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1247005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007966801"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2661", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008380407", 
              "https://doi.org/10.1038/nbt.2661"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2649", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008703730", 
              "https://doi.org/10.1038/nmeth.2649"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2623", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009511624", 
              "https://doi.org/10.1038/nbt.2623"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.162339.113", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010306401"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1247997", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010970903"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2647", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012543463", 
              "https://doi.org/10.1038/nbt.2647"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2477", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012600110", 
              "https://doi.org/10.1038/nbt.2477"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.089417.108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013259643"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2013.04.025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013935649"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.141", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014925028", 
              "https://doi.org/10.1038/cr.2013.141"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1755", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016004947", 
              "https://doi.org/10.1038/nbt.1755"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/febs.12735", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016915050"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2517", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017042091", 
              "https://doi.org/10.1038/nbt.2517"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.157", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017624741", 
              "https://doi.org/10.1038/cr.2013.157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biocel.2013.10.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018400217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1231143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019873131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1232033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022072971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms4157", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023186607", 
              "https://doi.org/10.1038/ncomms4157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2501", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024988605", 
              "https://doi.org/10.1038/nbt.2501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature13166", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027499136", 
              "https://doi.org/10.1038/nature13166"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2012.07.029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029001782"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2013.08.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034724285"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pgen.1002861", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036528847"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature13011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037342101", 
              "https://doi.org/10.1038/nature13011"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2014.02.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037467927"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2014.02.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037467927"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.r113.488247", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038183747"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.46", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038811201", 
              "https://doi.org/10.1038/cr.2013.46"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2014.11", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040674875", 
              "https://doi.org/10.1038/cr.2014.11"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jmcb/mjt047", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042484338"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2889", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043519930", 
              "https://doi.org/10.1038/nbt.2889"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2014.01.027", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046173829"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2652", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048853216", 
              "https://doi.org/10.1038/nbt.2652"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083154775", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083275505", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2015-05", 
        "datePublishedReg": "2015-05-01", 
        "description": "The rapid generation of various species and strains of laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, the dominant approach for genotyping of genome-modified animals has been the T7E1 endonuclease cleavage assay. Here, we present a polyacrylamide gel electrophoresis-based (PAGE) method to genotype mice harboring different types of indel mutations. We developed 6 strains of genome-modified mice using CRISPR/Cas9 system, and utilized this approach to genotype mice from F0 to F2 generation, which included single and multiplexed genome-modified mice. We also determined the maximal detection sensitivity for detecting mosaic DNA using PAGE-based assay as 0.5%. We further applied PAGE-based genotyping approach to detect CRISPR/Cas9-mediated on- and off-target effect in human 293T and induced pluripotent stem cells (iPSCs). Thus, PAGE-based genotyping approach meets the rapidly increasing demand for genotyping of the fast-growing number of genome-modified animals and human cell lines created using CRISPR/Cas9 system or other nuclease systems such as TALEN or ZFN. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/srep06420", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "4"
          }
        ], 
        "name": "An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System", 
        "pagination": "6420", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "30940e169bb44f657ceb019b9dcef27636955a3542f89a2bd55070697c272b85"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "25236476"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101563288"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/srep06420"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1010196515"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/srep06420", 
          "https://app.dimensions.ai/details/publication/pub.1010196515"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T20:53", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000549.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/srep06420"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep06420'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep06420'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep06420'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep06420'


     

    This table displays all metadata directly associated to this object as RDF triples.

    326 TRIPLES      21 PREDICATES      77 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/srep06420 schema:about N042cd534eda84f9bb0368344b0c5d9bc
    2 N138d2aeb3a664ce2887d6886d18c5909
    3 N4337273e5211405483cc607a0baa1db4
    4 N446a0126f22e4c2fa04f7177f70e693b
    5 N8a0103766081416daaa9b7852d7b8a9d
    6 Nbcfde7a437084f0ca3e0e0e4b6e480f1
    7 Nc1cc864a44b0416995c9283c939dce1e
    8 Ndcdd4a6908234c89bd887fb4337b3330
    9 Nf5f2f2e9cfa347e7886b912959fe43ea
    10 anzsrc-for:06
    11 anzsrc-for:0604
    12 schema:author N241983df56794ce1a20f3937f0e3eacc
    13 schema:citation sg:pub.10.1038/cr.2013.141
    14 sg:pub.10.1038/cr.2013.157
    15 sg:pub.10.1038/cr.2013.45
    16 sg:pub.10.1038/cr.2013.46
    17 sg:pub.10.1038/cr.2014.11
    18 sg:pub.10.1038/nature13011
    19 sg:pub.10.1038/nature13166
    20 sg:pub.10.1038/nbt.1755
    21 sg:pub.10.1038/nbt.2477
    22 sg:pub.10.1038/nbt.2501
    23 sg:pub.10.1038/nbt.2517
    24 sg:pub.10.1038/nbt.2623
    25 sg:pub.10.1038/nbt.2647
    26 sg:pub.10.1038/nbt.2652
    27 sg:pub.10.1038/nbt.2661
    28 sg:pub.10.1038/nbt.2889
    29 sg:pub.10.1038/nbt1319
    30 sg:pub.10.1038/ncomms4157
    31 sg:pub.10.1038/nmeth.2649
    32 https://app.dimensions.ai/details/publication/pub.1083154775
    33 https://app.dimensions.ai/details/publication/pub.1083275505
    34 https://doi.org/10.1016/j.bbadis.2014.04.020
    35 https://doi.org/10.1016/j.biocel.2013.10.010
    36 https://doi.org/10.1016/j.cell.2013.04.025
    37 https://doi.org/10.1016/j.cell.2013.08.022
    38 https://doi.org/10.1016/j.cell.2014.01.027
    39 https://doi.org/10.1016/j.molcel.2012.07.029
    40 https://doi.org/10.1016/j.ymeth.2014.02.009
    41 https://doi.org/10.1073/pnas.1400236111
    42 https://doi.org/10.1074/jbc.r113.488247
    43 https://doi.org/10.1093/jmcb/mjt047
    44 https://doi.org/10.1101/gr.089417.108
    45 https://doi.org/10.1101/gr.162339.113
    46 https://doi.org/10.1111/febs.12735
    47 https://doi.org/10.1126/science.1231143
    48 https://doi.org/10.1126/science.1232033
    49 https://doi.org/10.1126/science.1247005
    50 https://doi.org/10.1126/science.1247997
    51 https://doi.org/10.1371/journal.pgen.1002861
    52 schema:datePublished 2015-05
    53 schema:datePublishedReg 2015-05-01
    54 schema:description The rapid generation of various species and strains of laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, the dominant approach for genotyping of genome-modified animals has been the T7E1 endonuclease cleavage assay. Here, we present a polyacrylamide gel electrophoresis-based (PAGE) method to genotype mice harboring different types of indel mutations. We developed 6 strains of genome-modified mice using CRISPR/Cas9 system, and utilized this approach to genotype mice from F0 to F2 generation, which included single and multiplexed genome-modified mice. We also determined the maximal detection sensitivity for detecting mosaic DNA using PAGE-based assay as 0.5%. We further applied PAGE-based genotyping approach to detect CRISPR/Cas9-mediated on- and off-target effect in human 293T and induced pluripotent stem cells (iPSCs). Thus, PAGE-based genotyping approach meets the rapidly increasing demand for genotyping of the fast-growing number of genome-modified animals and human cell lines created using CRISPR/Cas9 system or other nuclease systems such as TALEN or ZFN.
    55 schema:genre research_article
    56 schema:inLanguage en
    57 schema:isAccessibleForFree true
    58 schema:isPartOf N37a7493456454398b5d721909efc47a1
    59 N6849ed659a16466bacbaebc41c65c80d
    60 sg:journal.1045337
    61 schema:name An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System
    62 schema:pagination 6420
    63 schema:productId N0c8f120415694cb99bcfbc089fda410b
    64 N35197fccca924550ba0dd036fe4c2940
    65 N38555cfee6c94365a610da20e9cfb1ba
    66 Nca25d332752a4652bf673aae448b5021
    67 Nfec71c1b86d940398e4b73905952f805
    68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010196515
    69 https://doi.org/10.1038/srep06420
    70 schema:sdDatePublished 2019-04-10T20:53
    71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    72 schema:sdPublisher N21766f23c1a4464bbdc9020a70551fca
    73 schema:url http://www.nature.com/articles/srep06420
    74 sgo:license sg:explorer/license/
    75 sgo:sdDataset articles
    76 rdf:type schema:ScholarlyArticle
    77 N0157c0e8f0214877bd19e53825da6f2b rdf:first sg:person.01211342764.54
    78 rdf:rest Na8cb880f49eb40d985c8fdb2c1cdb559
    79 N042cd534eda84f9bb0368344b0c5d9bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Genome
    81 rdf:type schema:DefinedTerm
    82 N0c8f120415694cb99bcfbc089fda410b schema:name pubmed_id
    83 schema:value 25236476
    84 rdf:type schema:PropertyValue
    85 N138d2aeb3a664ce2887d6886d18c5909 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Cell Line
    87 rdf:type schema:DefinedTerm
    88 N21766f23c1a4464bbdc9020a70551fca schema:name Springer Nature - SN SciGraph project
    89 rdf:type schema:Organization
    90 N241983df56794ce1a20f3937f0e3eacc rdf:first sg:person.01253311624.80
    91 rdf:rest N451dc65872af4aa0817d8dac1af5684d
    92 N29b3d3dc8d114c5db3478ae445c994b6 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    93 schema:familyName Zhang
    94 schema:givenName Xuewen
    95 rdf:type schema:Person
    96 N2c9f01690c15446788fcc2c8839e959c schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    97 schema:familyName Lu
    98 schema:givenName Lu
    99 rdf:type schema:Person
    100 N35197fccca924550ba0dd036fe4c2940 schema:name dimensions_id
    101 schema:value pub.1010196515
    102 rdf:type schema:PropertyValue
    103 N363c585a2c974061b89baaecfe0c5938 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    104 schema:familyName Cheng
    105 schema:givenName Jing
    106 rdf:type schema:Person
    107 N37a7493456454398b5d721909efc47a1 schema:volumeNumber 4
    108 rdf:type schema:PublicationVolume
    109 N37b5d6fa80a1476fa38c12d66bb049c6 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    110 schema:familyName Ding
    111 schema:givenName Mingqin
    112 rdf:type schema:Person
    113 N38555cfee6c94365a610da20e9cfb1ba schema:name doi
    114 schema:value 10.1038/srep06420
    115 rdf:type schema:PropertyValue
    116 N401e9eb0524c4965b6cfd34e709398e5 rdf:first sg:person.01367540224.07
    117 rdf:rest N9fbc87009bc042429ad32f55a3027b08
    118 N4337273e5211405483cc607a0baa1db4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name CRISPR-Cas Systems
    120 rdf:type schema:DefinedTerm
    121 N446a0126f22e4c2fa04f7177f70e693b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Animals
    123 rdf:type schema:DefinedTerm
    124 N451dc65872af4aa0817d8dac1af5684d rdf:first Ne46ae4cc54e04cb08fd58a72cfb3b124
    125 rdf:rest N401e9eb0524c4965b6cfd34e709398e5
    126 N4add925e9634445b8f7769fcff4e03f5 rdf:first sg:person.01321340020.08
    127 rdf:rest N7cca4d1714af42cf9aaeb2f54717f3b1
    128 N59f1583d77db4d088f779bdee44bf30e rdf:first N699918d2ed0748ce8deff730b4bb2b18
    129 rdf:rest Neb96a8072cf84936950dad961615a0fc
    130 N6849ed659a16466bacbaebc41c65c80d schema:issueNumber 1
    131 rdf:type schema:PublicationIssue
    132 N699918d2ed0748ce8deff730b4bb2b18 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    133 schema:familyName Song
    134 schema:givenName Guoxu
    135 rdf:type schema:Person
    136 N701f9240791346bcb01198ebdb22721f rdf:first N37b5d6fa80a1476fa38c12d66bb049c6
    137 rdf:rest Na16e28301e344ea5a03bd656ba8bd95b
    138 N7cca4d1714af42cf9aaeb2f54717f3b1 rdf:first sg:person.01132120163.89
    139 rdf:rest rdf:nil
    140 N8a0103766081416daaa9b7852d7b8a9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Animals, Genetically Modified
    142 rdf:type schema:DefinedTerm
    143 N8e62cb940bed47e7833b477efeb80ae2 rdf:first sg:person.01143227564.28
    144 rdf:rest N0157c0e8f0214877bd19e53825da6f2b
    145 N9c0abd585b5a4d109491de0960d951c7 rdf:first sg:person.01344216376.56
    146 rdf:rest N8e62cb940bed47e7833b477efeb80ae2
    147 N9fbc87009bc042429ad32f55a3027b08 rdf:first N2c9f01690c15446788fcc2c8839e959c
    148 rdf:rest N701f9240791346bcb01198ebdb22721f
    149 Na16e28301e344ea5a03bd656ba8bd95b rdf:first N363c585a2c974061b89baaecfe0c5938
    150 rdf:rest N59f1583d77db4d088f779bdee44bf30e
    151 Na8cb880f49eb40d985c8fdb2c1cdb559 rdf:first N29b3d3dc8d114c5db3478ae445c994b6
    152 rdf:rest N4add925e9634445b8f7769fcff4e03f5
    153 Nbcfde7a437084f0ca3e0e0e4b6e480f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Genotyping Techniques
    155 rdf:type schema:DefinedTerm
    156 Nc1cc864a44b0416995c9283c939dce1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    157 schema:name INDEL Mutation
    158 rdf:type schema:DefinedTerm
    159 Nca25d332752a4652bf673aae448b5021 schema:name nlm_unique_id
    160 schema:value 101563288
    161 rdf:type schema:PropertyValue
    162 Nca997e3bb0e04b9db05fbad9ca87677e schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    163 schema:familyName Gao
    164 schema:givenName Xing
    165 rdf:type schema:Person
    166 Ndcdd4a6908234c89bd887fb4337b3330 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name Humans
    168 rdf:type schema:DefinedTerm
    169 Ne46ae4cc54e04cb08fd58a72cfb3b124 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    170 schema:familyName Xu
    171 schema:givenName Yajie
    172 rdf:type schema:Person
    173 Neb96a8072cf84936950dad961615a0fc rdf:first Nca997e3bb0e04b9db05fbad9ca87677e
    174 rdf:rest N9c0abd585b5a4d109491de0960d951c7
    175 Nf5f2f2e9cfa347e7886b912959fe43ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    176 schema:name Mice
    177 rdf:type schema:DefinedTerm
    178 Nfec71c1b86d940398e4b73905952f805 schema:name readcube_id
    179 schema:value 30940e169bb44f657ceb019b9dcef27636955a3542f89a2bd55070697c272b85
    180 rdf:type schema:PropertyValue
    181 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    182 schema:name Biological Sciences
    183 rdf:type schema:DefinedTerm
    184 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    185 schema:name Genetics
    186 rdf:type schema:DefinedTerm
    187 sg:journal.1045337 schema:issn 2045-2322
    188 schema:name Scientific Reports
    189 rdf:type schema:Periodical
    190 sg:person.01132120163.89 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    191 schema:familyName Tian
    192 schema:givenName Yong
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132120163.89
    194 rdf:type schema:Person
    195 sg:person.01143227564.28 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    196 schema:familyName Fan
    197 schema:givenName Dongdong
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143227564.28
    199 rdf:type schema:Person
    200 sg:person.01211342764.54 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    201 schema:familyName Meng
    202 schema:givenName Shu
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211342764.54
    204 rdf:type schema:Person
    205 sg:person.01253311624.80 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    206 schema:familyName Zhu
    207 schema:givenName Xiaoxiao
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253311624.80
    209 rdf:type schema:Person
    210 sg:person.01321340020.08 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    211 schema:familyName Hu
    212 schema:givenName Shengdi
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321340020.08
    214 rdf:type schema:Person
    215 sg:person.01344216376.56 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    216 schema:familyName Yao
    217 schema:givenName Liangming
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01344216376.56
    219 rdf:type schema:Person
    220 sg:person.01367540224.07 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    221 schema:familyName Yu
    222 schema:givenName Shanshan
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367540224.07
    224 rdf:type schema:Person
    225 sg:pub.10.1038/cr.2013.141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014925028
    226 https://doi.org/10.1038/cr.2013.141
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1038/cr.2013.157 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017624741
    229 https://doi.org/10.1038/cr.2013.157
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1038/cr.2013.45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006654084
    232 https://doi.org/10.1038/cr.2013.45
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1038/cr.2013.46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038811201
    235 https://doi.org/10.1038/cr.2013.46
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1038/cr.2014.11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040674875
    238 https://doi.org/10.1038/cr.2014.11
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1038/nature13011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037342101
    241 https://doi.org/10.1038/nature13011
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1038/nature13166 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027499136
    244 https://doi.org/10.1038/nature13166
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1038/nbt.1755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016004947
    247 https://doi.org/10.1038/nbt.1755
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1038/nbt.2477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012600110
    250 https://doi.org/10.1038/nbt.2477
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/nbt.2501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024988605
    253 https://doi.org/10.1038/nbt.2501
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/nbt.2517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017042091
    256 https://doi.org/10.1038/nbt.2517
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/nbt.2623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009511624
    259 https://doi.org/10.1038/nbt.2623
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/nbt.2647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012543463
    262 https://doi.org/10.1038/nbt.2647
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1038/nbt.2652 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048853216
    265 https://doi.org/10.1038/nbt.2652
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/nbt.2661 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008380407
    268 https://doi.org/10.1038/nbt.2661
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/nbt.2889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043519930
    271 https://doi.org/10.1038/nbt.2889
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1038/nbt1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002154526
    274 https://doi.org/10.1038/nbt1319
    275 rdf:type schema:CreativeWork
    276 sg:pub.10.1038/ncomms4157 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023186607
    277 https://doi.org/10.1038/ncomms4157
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1038/nmeth.2649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008703730
    280 https://doi.org/10.1038/nmeth.2649
    281 rdf:type schema:CreativeWork
    282 https://app.dimensions.ai/details/publication/pub.1083154775 schema:CreativeWork
    283 https://app.dimensions.ai/details/publication/pub.1083275505 schema:CreativeWork
    284 https://doi.org/10.1016/j.bbadis.2014.04.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006504293
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1016/j.biocel.2013.10.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018400217
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1016/j.cell.2013.04.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013935649
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1016/j.cell.2013.08.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034724285
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1016/j.cell.2014.01.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046173829
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1016/j.molcel.2012.07.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029001782
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1016/j.ymeth.2014.02.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037467927
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1073/pnas.1400236111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002427034
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1074/jbc.r113.488247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038183747
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1093/jmcb/mjt047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042484338
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1101/gr.089417.108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013259643
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1101/gr.162339.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010306401
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1111/febs.12735 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016915050
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1126/science.1231143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019873131
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1126/science.1232033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022072971
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1126/science.1247005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007966801
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1126/science.1247997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010970903
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.1371/journal.pgen.1002861 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036528847
    319 rdf:type schema:CreativeWork
    320 https://www.grid.ac/institutes/grid.410726.6 schema:alternateName University of Chinese Academy of Sciences
    321 schema:name Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    322 University of Chinese Academy of Sciences, Beijing 100080, China
    323 rdf:type schema:Organization
    324 https://www.grid.ac/institutes/grid.418856.6 schema:alternateName Institute of Biophysics
    325 schema:name Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    326 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...