Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-05

AUTHORS

Tetsushi Sakuma, Ayami Nishikawa, Satoshi Kume, Kazuaki Chayama, Takashi Yamamoto

ABSTRACT

CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette. We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. Our system provides an efficient targeting strategy for multiplex genome/epigenome editing, simultaneous activation/repression of multiple genes, and beyond. More... »

PAGES

5400

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep05400

DOI

http://dx.doi.org/10.1038/srep05400

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043480198

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24954249


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