Chronic activation of protein kinase Bβ/Akt2 leads to multinucleation and cell fusion in human epithelial kidney cells: events associated with ... View Full Text


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Article Info

DATE

2005-06-13

AUTHORS

Jing Jin, James R Woodgett

ABSTRACT

Most cancers arise from the stepwise accumulation of genetic changes. There is also evidence for defects in the machinery and checkpoints for maintenance of normal diploid chromosome complements, resulting in genetic instability that helps fuel the accumulation of mutations that contribute to the development of cancer. The proto-oncogene protein kinase B (PKB/Akt), and its regulators including phosphatidylinositol 3′ kinase and PTEN, has been shown to play critical roles in the regulation of multiple cellular functions such as transcription, cell survival, cell cycle progression, angiogenesis and cell motility – all of which are important to the malignant process. Here, we report the use of a membrane targeted PKBβ, the activation of which is under the control of a 4-hydroxy-Tamoxifen-responsive estrogen-receptor (ER) ligand binding domain. Induction of PKBβ-ER activity in human kidney epithelial cells (HEK293) resulted in changes in cellular growth, size, and in the appearance of aneuploid cells. Over time, in a PKBβ-dependent manner, cells also underwent extensive multinucleation caused due to a combination of both endomitosis and cell fusion. These findings suggest that chronic activation of PKBβ may contribute to genetic instability and autophagy, properties commonly found in tumor cells. More... »

PAGES

5459-5470

References to SciGraph publications

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  • 1995-12. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B in NATURE
  • 1997-05-08. A putative serine/threonine kinase encoding gene BTAK on chromosome 20q13 is amplified and overexpressed in human breast cancer cell lines in ONCOGENE
  • 2003-09-10. Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosome in BMC MOLECULAR AND CELL BIOLOGY
  • 2001-10. PKB/AKT: functional insights from genetic models in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2001-03-20. Tumour-amplified kinase BTAK is amplified and overexpressed in gastric cancers with possible involvement in aneuploid formation in BRITISH JOURNAL OF CANCER
  • 2001-08. Mycobacterium tuberculosis: here today, and here tomorrow in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2002-05-16. AKT2 is frequently upregulated in HER-2/neu-positive breast cancers and may contribute to tumor aggressiveness by enhancing cell survival in ONCOGENE
  • 2004-01. From polyploidy to aneuploidy, genome instability and cancer in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2004. Phosphoinositide Involvement in Phagocytosis and Phagosome Maturation in PHOSPHOINOSITIDES IN SUBCELLULAR TARGETING AND ENZYME ACTIVATION
  • 1998-10. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation in NATURE GENETICS
  • 2002-07-01. The phosphatidylinositol 3-Kinase–AKT pathway in human cancer in NATURE REVIEWS CANCER
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    URI

    http://scigraph.springernature.com/pub.10.1038/sj.onc.1208704

    DOI

    http://dx.doi.org/10.1038/sj.onc.1208704

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025170629

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16007218


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