Ontology type: schema:ScholarlyArticle Open Access: True
2003-02-13
AUTHORSY Shimizu, N Yamamichi, K Saitoh, A Watanabe, T Ito, M Yamamichi-Nishina, M Mizutani, N Yahagi, T Suzuki, C Sasakawa, S Yasugi, M Ichinose, H Iba
ABSTRACTThe oncogene function in primary epithelial cells is largely unclear. Recombination organ cultures in combination with the stable and transient gene transfer techniques by retrovirus and electroporation, respectively, enable us to transfer oncogenes specifically into primary epithelial cells of the developing avian glandular stomach (proventriculus). In this system, the epithelium and mesenchyme are mutually dependent on each other for their growth and differentiation. We report here that either stable or transient expression of v-src in the epithelium causes budding and migration of epithelial cells into mesenchyme. In response to the transient expression of v-Src or a constitutive active mutant of MEK, we observed immediate downregulation of the Sonic hedgehog gene and subsequent elimination of E-cadherine expression in migrating cells, suggesting the involvement of MAP kinase signaling pathway in these processes. v-src-expressing cells that were retained in the epithelium underwent apoptosis (anoikis) and detached from the culture. Continuous expression of v-src by, for example, Rous sarcoma virus (RSV) was required for the epithelial cells to acquire the ability to express type I collagen and fibronectin genes (mesenchymal markers), and finally to establish the epithelial–mesenchymal transition. These observations would partly explain why RSV does not apparently cause carcinoma formation, but induces sarcomas exclusively. More... »
PAGES884-893
http://scigraph.springernature.com/pub.10.1038/sj.onc.1206174
DOIhttp://dx.doi.org/10.1038/sj.onc.1206174
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1017442422
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/12584568
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Clinical Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Oncology and Carcinogenesis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Avian Sarcoma Viruses",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Transformation, Neoplastic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chick Embryo",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Coturnix",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Epithelium",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gastric Mucosa",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Profiling",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Transfer Techniques",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Kinetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mesoderm",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Oncogene Protein pp60(v-src)",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protein Serine-Threonine Kinases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Signal Transduction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Stomach",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Second Department of Internal Medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan",
"id": "http://www.grid.ac/institutes/grid.412857.d",
"name": [
"Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"Second Department of Internal Medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan"
],
"type": "Organization"
},
"familyName": "Shimizu",
"givenName": "Y",
"id": "sg:person.01354407403.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354407403.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Yamamichi",
"givenName": "N",
"id": "sg:person.0575533506.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575533506.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Saitoh",
"givenName": "K",
"id": "sg:person.0761102661.85",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761102661.85"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Watanabe",
"givenName": "A",
"id": "sg:person.0665553145.88",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665553145.88"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Ito",
"givenName": "T",
"id": "sg:person.01177075377.58",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177075377.58"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Yamamichi-Nishina",
"givenName": "M",
"id": "sg:person.01071140722.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071140722.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Laboratory Animal Research Station, Nippon Institute for Biological Science, 3331, Kamisasao, 408-0041, Kobuchizawa, Yamanashi, Japan",
"id": "http://www.grid.ac/institutes/grid.420033.6",
"name": [
"Laboratory Animal Research Station, Nippon Institute for Biological Science, 3331, Kamisasao, 408-0041, Kobuchizawa, Yamanashi, Japan"
],
"type": "Organization"
},
"familyName": "Mizutani",
"givenName": "M",
"id": "sg:person.01145351033.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145351033.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology, Faculty of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Department of Gastroenterology, Faculty of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Yahagi",
"givenName": "N",
"id": "sg:person.01134761532.44",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134761532.44"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Suzuki",
"givenName": "T",
"id": "sg:person.016705511144.35",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016705511144.35"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Sasakawa",
"givenName": "C",
"id": "sg:person.0602051475.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602051475.41"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, 192-0397, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.265074.2",
"name": [
"Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, 192-0397, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Yasugi",
"givenName": "S",
"id": "sg:person.0733740407.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733740407.52"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Second Department of Internal medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan",
"id": "http://www.grid.ac/institutes/grid.412857.d",
"name": [
"Second Department of Internal medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan"
],
"type": "Organization"
},
"familyName": "Ichinose",
"givenName": "M",
"id": "sg:person.01335107416.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335107416.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.26999.3d",
"name": [
"Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Iba",
"givenName": "H",
"id": "sg:person.01002001545.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002001545.54"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1023/a:1026469522868",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003822972",
"https://doi.org/10.1023/a:1026469522868"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/5971",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004991778",
"https://doi.org/10.1038/5971"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201675",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053101278",
"https://doi.org/10.1038/sj.onc.1201675"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201077",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012603705",
"https://doi.org/10.1038/sj.onc.1201077"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf01096165",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005419590",
"https://doi.org/10.1007/bf01096165"
],
"type": "CreativeWork"
}
],
"datePublished": "2003-02-13",
"datePublishedReg": "2003-02-13",
"description": "The oncogene function in primary epithelial cells is largely unclear. Recombination organ cultures in combination with the stable and transient gene transfer techniques by retrovirus and electroporation, respectively, enable us to transfer oncogenes specifically into primary epithelial cells of the developing avian glandular stomach (proventriculus). In this system, the epithelium and mesenchyme are mutually dependent on each other for their growth and differentiation. We report here that either stable or transient expression of v-src in the epithelium causes budding and migration of epithelial cells into mesenchyme. In response to the transient expression of v-Src or a constitutive active mutant of MEK, we observed immediate downregulation of the Sonic hedgehog gene and subsequent elimination of E-cadherine expression in migrating cells, suggesting the involvement of MAP kinase signaling pathway in these processes. v-src-expressing cells that were retained in the epithelium underwent apoptosis (anoikis) and detached from the culture. Continuous expression of v-src by, for example, Rous sarcoma virus (RSV) was required for the epithelial cells to acquire the ability to express type I collagen and fibronectin genes (mesenchymal markers), and finally to establish the epithelial\u2013mesenchymal transition. These observations would partly explain why RSV does not apparently cause carcinoma formation, but induces sarcomas exclusively.",
"genre": "article",
"id": "sg:pub.10.1038/sj.onc.1206174",
"inLanguage": "en",
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1097543",
"issn": [
"0950-9232",
"1476-5594"
],
"name": "Oncogene",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "6",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "22"
}
],
"keywords": [
"epithelial-mesenchymal transition",
"epithelial cells",
"primary epithelial cells",
"glandular stomach",
"Rous sarcoma virus",
"carcinoma formation",
"organ culture",
"oncogene function",
"stomach",
"type I",
"epithelium",
"gene transfer techniques",
"MAP kinase",
"cells",
"v-Src-expressing cells",
"sonic hedgehog gene",
"transient expression",
"expression",
"continuous expression",
"sarcoma virus",
"constitutive active mutant",
"sarcoma",
"mesenchyme",
"v-Src",
"downregulation",
"virus",
"immediate downregulation",
"apoptosis",
"cause",
"genes",
"active mutant",
"oncogene",
"hedgehog gene",
"subsequent elimination",
"fibronectin gene",
"involvement",
"retroviruses",
"MEK",
"culture",
"pathway",
"response",
"differentiation",
"kinase",
"transfer technique",
"electroporation",
"elimination",
"migration",
"combination",
"function",
"ability",
"growth",
"mutants",
"observations",
"technique",
"formation",
"system",
"kinetics",
"process",
"transition",
"example"
],
"name": "Kinetics of v-src-induced epithelial\u2013mesenchymal transition in developing glandular stomach",
"pagination": "884-893",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1017442422"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/sj.onc.1206174"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"12584568"
]
}
],
"sameAs": [
"https://doi.org/10.1038/sj.onc.1206174",
"https://app.dimensions.ai/details/publication/pub.1017442422"
],
"sdDataset": "articles",
"sdDatePublished": "2022-06-01T22:05",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_376.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/sj.onc.1206174"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'
This table displays all metadata directly associated to this object as RDF triples.
306 TRIPLES
22 PREDICATES
107 URIs
93 LITERALS
22 BLANK NODES