Kinetics of v-src-induced epithelial–mesenchymal transition in developing glandular stomach View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-02-13

AUTHORS

Y Shimizu, N Yamamichi, K Saitoh, A Watanabe, T Ito, M Yamamichi-Nishina, M Mizutani, N Yahagi, T Suzuki, C Sasakawa, S Yasugi, M Ichinose, H Iba

ABSTRACT

The oncogene function in primary epithelial cells is largely unclear. Recombination organ cultures in combination with the stable and transient gene transfer techniques by retrovirus and electroporation, respectively, enable us to transfer oncogenes specifically into primary epithelial cells of the developing avian glandular stomach (proventriculus). In this system, the epithelium and mesenchyme are mutually dependent on each other for their growth and differentiation. We report here that either stable or transient expression of v-src in the epithelium causes budding and migration of epithelial cells into mesenchyme. In response to the transient expression of v-Src or a constitutive active mutant of MEK, we observed immediate downregulation of the Sonic hedgehog gene and subsequent elimination of E-cadherine expression in migrating cells, suggesting the involvement of MAP kinase signaling pathway in these processes. v-src-expressing cells that were retained in the epithelium underwent apoptosis (anoikis) and detached from the culture. Continuous expression of v-src by, for example, Rous sarcoma virus (RSV) was required for the epithelial cells to acquire the ability to express type I collagen and fibronectin genes (mesenchymal markers), and finally to establish the epithelial–mesenchymal transition. These observations would partly explain why RSV does not apparently cause carcinoma formation, but induces sarcomas exclusively. More... »

PAGES

884-893

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.onc.1206174

DOI

http://dx.doi.org/10.1038/sj.onc.1206174

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017442422

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12584568


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Avian Sarcoma Viruses", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Transformation, Neoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chick Embryo", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coturnix", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gastric Mucosa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesoderm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oncogene Protein pp60(v-src)", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Serine-Threonine Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stomach", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Second Department of Internal Medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan", 
          "id": "http://www.grid.ac/institutes/grid.412857.d", 
          "name": [
            "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
            "Second Department of Internal Medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shimizu", 
        "givenName": "Y", 
        "id": "sg:person.01354407403.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354407403.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamichi", 
        "givenName": "N", 
        "id": "sg:person.0575533506.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575533506.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saitoh", 
        "givenName": "K", 
        "id": "sg:person.0761102661.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761102661.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watanabe", 
        "givenName": "A", 
        "id": "sg:person.0665553145.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665553145.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ito", 
        "givenName": "T", 
        "id": "sg:person.01177075377.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177075377.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamichi-Nishina", 
        "givenName": "M", 
        "id": "sg:person.01071140722.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071140722.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory Animal Research Station, Nippon Institute for Biological Science, 3331, Kamisasao, 408-0041, Kobuchizawa, Yamanashi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.420033.6", 
          "name": [
            "Laboratory Animal Research Station, Nippon Institute for Biological Science, 3331, Kamisasao, 408-0041, Kobuchizawa, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mizutani", 
        "givenName": "M", 
        "id": "sg:person.01145351033.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145351033.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Gastroenterology, Faculty of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Gastroenterology, Faculty of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yahagi", 
        "givenName": "N", 
        "id": "sg:person.01134761532.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134761532.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suzuki", 
        "givenName": "T", 
        "id": "sg:person.016705511144.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016705511144.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sasakawa", 
        "givenName": "C", 
        "id": "sg:person.0602051475.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602051475.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, 192-0397, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.265074.2", 
          "name": [
            "Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, 192-0397, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yasugi", 
        "givenName": "S", 
        "id": "sg:person.0733740407.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733740407.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Department of Internal medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan", 
          "id": "http://www.grid.ac/institutes/grid.412857.d", 
          "name": [
            "Second Department of Internal medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ichinose", 
        "givenName": "M", 
        "id": "sg:person.01335107416.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335107416.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Division of Host\u2013Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Iba", 
        "givenName": "H", 
        "id": "sg:person.01002001545.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002001545.54"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1023/a:1026469522868", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003822972", 
          "https://doi.org/10.1023/a:1026469522868"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/5971", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004991778", 
          "https://doi.org/10.1038/5971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201675", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053101278", 
          "https://doi.org/10.1038/sj.onc.1201675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201077", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012603705", 
          "https://doi.org/10.1038/sj.onc.1201077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01096165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005419590", 
          "https://doi.org/10.1007/bf01096165"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-02-13", 
    "datePublishedReg": "2003-02-13", 
    "description": "The oncogene function in primary epithelial cells is largely unclear. Recombination organ cultures in combination with the stable and transient gene transfer techniques by retrovirus and electroporation, respectively, enable us to transfer oncogenes specifically into primary epithelial cells of the developing avian glandular stomach (proventriculus). In this system, the epithelium and mesenchyme are mutually dependent on each other for their growth and differentiation. We report here that either stable or transient expression of v-src in the epithelium causes budding and migration of epithelial cells into mesenchyme. In response to the transient expression of v-Src or a constitutive active mutant of MEK, we observed immediate downregulation of the Sonic hedgehog gene and subsequent elimination of E-cadherine expression in migrating cells, suggesting the involvement of MAP kinase signaling pathway in these processes. v-src-expressing cells that were retained in the epithelium underwent apoptosis (anoikis) and detached from the culture. Continuous expression of v-src by, for example, Rous sarcoma virus (RSV) was required for the epithelial cells to acquire the ability to express type I collagen and fibronectin genes (mesenchymal markers), and finally to establish the epithelial\u2013mesenchymal transition. These observations would partly explain why RSV does not apparently cause carcinoma formation, but induces sarcomas exclusively.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/sj.onc.1206174", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1097543", 
        "issn": [
          "0950-9232", 
          "1476-5594"
        ], 
        "name": "Oncogene", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "22"
      }
    ], 
    "keywords": [
      "epithelial-mesenchymal transition", 
      "epithelial cells", 
      "primary epithelial cells", 
      "glandular stomach", 
      "Rous sarcoma virus", 
      "carcinoma formation", 
      "organ culture", 
      "oncogene function", 
      "stomach", 
      "type I", 
      "epithelium", 
      "gene transfer techniques", 
      "MAP kinase", 
      "cells", 
      "v-Src-expressing cells", 
      "sonic hedgehog gene", 
      "transient expression", 
      "expression", 
      "continuous expression", 
      "sarcoma virus", 
      "constitutive active mutant", 
      "sarcoma", 
      "mesenchyme", 
      "v-Src", 
      "downregulation", 
      "virus", 
      "immediate downregulation", 
      "apoptosis", 
      "cause", 
      "genes", 
      "active mutant", 
      "oncogene", 
      "hedgehog gene", 
      "subsequent elimination", 
      "fibronectin gene", 
      "involvement", 
      "retroviruses", 
      "MEK", 
      "culture", 
      "pathway", 
      "response", 
      "differentiation", 
      "kinase", 
      "transfer technique", 
      "electroporation", 
      "elimination", 
      "migration", 
      "combination", 
      "function", 
      "ability", 
      "growth", 
      "mutants", 
      "observations", 
      "technique", 
      "formation", 
      "system", 
      "kinetics", 
      "process", 
      "transition", 
      "example"
    ], 
    "name": "Kinetics of v-src-induced epithelial\u2013mesenchymal transition in developing glandular stomach", 
    "pagination": "884-893", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1017442422"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.onc.1206174"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12584568"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.onc.1206174", 
      "https://app.dimensions.ai/details/publication/pub.1017442422"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-06-01T22:05", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_376.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/sj.onc.1206174"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1206174'


 

This table displays all metadata directly associated to this object as RDF triples.

306 TRIPLES      22 PREDICATES      107 URIs      93 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.onc.1206174 schema:about N3a649355a8e146bbb0688db4c0f5ad93
2 N4a401b712c824beb9ebd26e702cc226d
3 N4e0b8ab7056740c3bb4c0685929207bd
4 N51245e8b341342febc41b399bdb20f76
5 N7b04135a109247daae80060519f24eb6
6 N8226fc8e1f71460c8eacb8f21344ae8b
7 N879ec1b80b6c4cdfabec31afed34839d
8 N8ae2c06ff5bc4a9bb72dcc8553d59db9
9 N992b49f0f8cd4e4495de842af00065e7
10 N9c427d3892df473ba7aa377d339df28e
11 N9ec20f750bf541cf90fdfc37780baad2
12 Nbeba82c06f6b4915a8568064eb990fb3
13 Nd36564dab228442f89707a2514e4a3e0
14 Necf2f18fe8b948c391965f42c71ac157
15 Nf54cc100ddba426db584c99ec20c1ad0
16 anzsrc-for:11
17 anzsrc-for:1103
18 anzsrc-for:1112
19 schema:author Na863a1c8ea1a42eabb8b0fb883c89b8a
20 schema:citation sg:pub.10.1007/bf01096165
21 sg:pub.10.1023/a:1026469522868
22 sg:pub.10.1038/5971
23 sg:pub.10.1038/sj.onc.1201077
24 sg:pub.10.1038/sj.onc.1201675
25 schema:datePublished 2003-02-13
26 schema:datePublishedReg 2003-02-13
27 schema:description The oncogene function in primary epithelial cells is largely unclear. Recombination organ cultures in combination with the stable and transient gene transfer techniques by retrovirus and electroporation, respectively, enable us to transfer oncogenes specifically into primary epithelial cells of the developing avian glandular stomach (proventriculus). In this system, the epithelium and mesenchyme are mutually dependent on each other for their growth and differentiation. We report here that either stable or transient expression of v-src in the epithelium causes budding and migration of epithelial cells into mesenchyme. In response to the transient expression of v-Src or a constitutive active mutant of MEK, we observed immediate downregulation of the Sonic hedgehog gene and subsequent elimination of E-cadherine expression in migrating cells, suggesting the involvement of MAP kinase signaling pathway in these processes. v-src-expressing cells that were retained in the epithelium underwent apoptosis (anoikis) and detached from the culture. Continuous expression of v-src by, for example, Rous sarcoma virus (RSV) was required for the epithelial cells to acquire the ability to express type I collagen and fibronectin genes (mesenchymal markers), and finally to establish the epithelial–mesenchymal transition. These observations would partly explain why RSV does not apparently cause carcinoma formation, but induces sarcomas exclusively.
28 schema:genre article
29 schema:inLanguage en
30 schema:isAccessibleForFree true
31 schema:isPartOf N3eacc905678542f9bac58739bbf8a232
32 Nbb0e0cab8cf2443eb4d1c55dab9557ab
33 sg:journal.1097543
34 schema:keywords MAP kinase
35 MEK
36 Rous sarcoma virus
37 ability
38 active mutant
39 apoptosis
40 carcinoma formation
41 cause
42 cells
43 combination
44 constitutive active mutant
45 continuous expression
46 culture
47 differentiation
48 downregulation
49 electroporation
50 elimination
51 epithelial cells
52 epithelial-mesenchymal transition
53 epithelium
54 example
55 expression
56 fibronectin gene
57 formation
58 function
59 gene transfer techniques
60 genes
61 glandular stomach
62 growth
63 hedgehog gene
64 immediate downregulation
65 involvement
66 kinase
67 kinetics
68 mesenchyme
69 migration
70 mutants
71 observations
72 oncogene
73 oncogene function
74 organ culture
75 pathway
76 primary epithelial cells
77 process
78 response
79 retroviruses
80 sarcoma
81 sarcoma virus
82 sonic hedgehog gene
83 stomach
84 subsequent elimination
85 system
86 technique
87 transfer technique
88 transient expression
89 transition
90 type I
91 v-Src
92 v-Src-expressing cells
93 virus
94 schema:name Kinetics of v-src-induced epithelial–mesenchymal transition in developing glandular stomach
95 schema:pagination 884-893
96 schema:productId N158c8c59eb6e4817bd7b58753ed417a1
97 N3c7f5a53d4854d8aa162021158175fc8
98 N8df266b1d8d8437791cfe9ab5472d040
99 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017442422
100 https://doi.org/10.1038/sj.onc.1206174
101 schema:sdDatePublished 2022-06-01T22:05
102 schema:sdLicense https://scigraph.springernature.com/explorer/license/
103 schema:sdPublisher N9071cde6c76d45e186cf72c9fda885ad
104 schema:url https://doi.org/10.1038/sj.onc.1206174
105 sgo:license sg:explorer/license/
106 sgo:sdDataset articles
107 rdf:type schema:ScholarlyArticle
108 N0bdc18ae6ab5444f900292424c2de803 rdf:first sg:person.0602051475.41
109 rdf:rest N11d3e738093043fd8c49b7c651db1fe4
110 N11d3e738093043fd8c49b7c651db1fe4 rdf:first sg:person.0733740407.52
111 rdf:rest N1680fb7b02224c80b0354f506cd5ed16
112 N158c8c59eb6e4817bd7b58753ed417a1 schema:name pubmed_id
113 schema:value 12584568
114 rdf:type schema:PropertyValue
115 N1680fb7b02224c80b0354f506cd5ed16 rdf:first sg:person.01335107416.23
116 rdf:rest N3a37a133aac140e8a05710bc66cf024e
117 N279eb403191a4635b743882614984cb9 rdf:first sg:person.0761102661.85
118 rdf:rest N7040e1de924a4c5e885d265b804ce450
119 N3a37a133aac140e8a05710bc66cf024e rdf:first sg:person.01002001545.54
120 rdf:rest rdf:nil
121 N3a649355a8e146bbb0688db4c0f5ad93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Protein Serine-Threonine Kinases
123 rdf:type schema:DefinedTerm
124 N3c7f5a53d4854d8aa162021158175fc8 schema:name dimensions_id
125 schema:value pub.1017442422
126 rdf:type schema:PropertyValue
127 N3eacc905678542f9bac58739bbf8a232 schema:volumeNumber 22
128 rdf:type schema:PublicationVolume
129 N4a401b712c824beb9ebd26e702cc226d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Oncogene Protein pp60(v-src)
131 rdf:type schema:DefinedTerm
132 N4e0b8ab7056740c3bb4c0685929207bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Epithelium
134 rdf:type schema:DefinedTerm
135 N51245e8b341342febc41b399bdb20f76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Animals
137 rdf:type schema:DefinedTerm
138 N5b1cf04e6de74a33913c36ce22a6f534 rdf:first sg:person.01071140722.19
139 rdf:rest Nc8be81fbc1d84544b9b100adfb9b4354
140 N7040e1de924a4c5e885d265b804ce450 rdf:first sg:person.0665553145.88
141 rdf:rest N88348e99d76d48cca3ebeaa4fa81e423
142 N7b04135a109247daae80060519f24eb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Signal Transduction
144 rdf:type schema:DefinedTerm
145 N8226fc8e1f71460c8eacb8f21344ae8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Stomach
147 rdf:type schema:DefinedTerm
148 N869ba3c5d818456394a8557740beab4c rdf:first sg:person.01134761532.44
149 rdf:rest Na53287aee7314e3fb42469d2cc40efc9
150 N879ec1b80b6c4cdfabec31afed34839d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Gene Expression Profiling
152 rdf:type schema:DefinedTerm
153 N88348e99d76d48cca3ebeaa4fa81e423 rdf:first sg:person.01177075377.58
154 rdf:rest N5b1cf04e6de74a33913c36ce22a6f534
155 N8ae2c06ff5bc4a9bb72dcc8553d59db9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Kinetics
157 rdf:type schema:DefinedTerm
158 N8df266b1d8d8437791cfe9ab5472d040 schema:name doi
159 schema:value 10.1038/sj.onc.1206174
160 rdf:type schema:PropertyValue
161 N9071cde6c76d45e186cf72c9fda885ad schema:name Springer Nature - SN SciGraph project
162 rdf:type schema:Organization
163 N97c67e54115247af87cd00ba6bed0983 rdf:first sg:person.0575533506.47
164 rdf:rest N279eb403191a4635b743882614984cb9
165 N992b49f0f8cd4e4495de842af00065e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Gastric Mucosa
167 rdf:type schema:DefinedTerm
168 N9c427d3892df473ba7aa377d339df28e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Chick Embryo
170 rdf:type schema:DefinedTerm
171 N9ec20f750bf541cf90fdfc37780baad2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Coturnix
173 rdf:type schema:DefinedTerm
174 Na53287aee7314e3fb42469d2cc40efc9 rdf:first sg:person.016705511144.35
175 rdf:rest N0bdc18ae6ab5444f900292424c2de803
176 Na863a1c8ea1a42eabb8b0fb883c89b8a rdf:first sg:person.01354407403.19
177 rdf:rest N97c67e54115247af87cd00ba6bed0983
178 Nbb0e0cab8cf2443eb4d1c55dab9557ab schema:issueNumber 6
179 rdf:type schema:PublicationIssue
180 Nbeba82c06f6b4915a8568064eb990fb3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Cell Transformation, Neoplastic
182 rdf:type schema:DefinedTerm
183 Nc8be81fbc1d84544b9b100adfb9b4354 rdf:first sg:person.01145351033.23
184 rdf:rest N869ba3c5d818456394a8557740beab4c
185 Nd36564dab228442f89707a2514e4a3e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Mesoderm
187 rdf:type schema:DefinedTerm
188 Necf2f18fe8b948c391965f42c71ac157 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Avian Sarcoma Viruses
190 rdf:type schema:DefinedTerm
191 Nf54cc100ddba426db584c99ec20c1ad0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Gene Transfer Techniques
193 rdf:type schema:DefinedTerm
194 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
195 schema:name Medical and Health Sciences
196 rdf:type schema:DefinedTerm
197 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
198 schema:name Clinical Sciences
199 rdf:type schema:DefinedTerm
200 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
201 schema:name Oncology and Carcinogenesis
202 rdf:type schema:DefinedTerm
203 sg:journal.1097543 schema:issn 0950-9232
204 1476-5594
205 schema:name Oncogene
206 schema:publisher Springer Nature
207 rdf:type schema:Periodical
208 sg:person.01002001545.54 schema:affiliation grid-institutes:grid.26999.3d
209 schema:familyName Iba
210 schema:givenName H
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002001545.54
212 rdf:type schema:Person
213 sg:person.01071140722.19 schema:affiliation grid-institutes:grid.26999.3d
214 schema:familyName Yamamichi-Nishina
215 schema:givenName M
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071140722.19
217 rdf:type schema:Person
218 sg:person.01134761532.44 schema:affiliation grid-institutes:grid.26999.3d
219 schema:familyName Yahagi
220 schema:givenName N
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134761532.44
222 rdf:type schema:Person
223 sg:person.01145351033.23 schema:affiliation grid-institutes:grid.420033.6
224 schema:familyName Mizutani
225 schema:givenName M
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145351033.23
227 rdf:type schema:Person
228 sg:person.01177075377.58 schema:affiliation grid-institutes:grid.26999.3d
229 schema:familyName Ito
230 schema:givenName T
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177075377.58
232 rdf:type schema:Person
233 sg:person.01335107416.23 schema:affiliation grid-institutes:grid.412857.d
234 schema:familyName Ichinose
235 schema:givenName M
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335107416.23
237 rdf:type schema:Person
238 sg:person.01354407403.19 schema:affiliation grid-institutes:grid.412857.d
239 schema:familyName Shimizu
240 schema:givenName Y
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354407403.19
242 rdf:type schema:Person
243 sg:person.016705511144.35 schema:affiliation grid-institutes:grid.26999.3d
244 schema:familyName Suzuki
245 schema:givenName T
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016705511144.35
247 rdf:type schema:Person
248 sg:person.0575533506.47 schema:affiliation grid-institutes:grid.26999.3d
249 schema:familyName Yamamichi
250 schema:givenName N
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575533506.47
252 rdf:type schema:Person
253 sg:person.0602051475.41 schema:affiliation grid-institutes:grid.26999.3d
254 schema:familyName Sasakawa
255 schema:givenName C
256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602051475.41
257 rdf:type schema:Person
258 sg:person.0665553145.88 schema:affiliation grid-institutes:grid.26999.3d
259 schema:familyName Watanabe
260 schema:givenName A
261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665553145.88
262 rdf:type schema:Person
263 sg:person.0733740407.52 schema:affiliation grid-institutes:grid.265074.2
264 schema:familyName Yasugi
265 schema:givenName S
266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733740407.52
267 rdf:type schema:Person
268 sg:person.0761102661.85 schema:affiliation grid-institutes:grid.26999.3d
269 schema:familyName Saitoh
270 schema:givenName K
271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761102661.85
272 rdf:type schema:Person
273 sg:pub.10.1007/bf01096165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005419590
274 https://doi.org/10.1007/bf01096165
275 rdf:type schema:CreativeWork
276 sg:pub.10.1023/a:1026469522868 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003822972
277 https://doi.org/10.1023/a:1026469522868
278 rdf:type schema:CreativeWork
279 sg:pub.10.1038/5971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004991778
280 https://doi.org/10.1038/5971
281 rdf:type schema:CreativeWork
282 sg:pub.10.1038/sj.onc.1201077 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012603705
283 https://doi.org/10.1038/sj.onc.1201077
284 rdf:type schema:CreativeWork
285 sg:pub.10.1038/sj.onc.1201675 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053101278
286 https://doi.org/10.1038/sj.onc.1201675
287 rdf:type schema:CreativeWork
288 grid-institutes:grid.265074.2 schema:alternateName Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, 192-0397, Tokyo, Japan
289 schema:name Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, 192-0397, Tokyo, Japan
290 rdf:type schema:Organization
291 grid-institutes:grid.26999.3d schema:alternateName Department of Gastroenterology, Faculty of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan
292 Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan
293 Division of Host–Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan
294 schema:name Department of Gastroenterology, Faculty of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan
295 Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan
296 Division of Host–Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan
297 rdf:type schema:Organization
298 grid-institutes:grid.412857.d schema:alternateName Second Department of Internal Medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan
299 Second Department of Internal medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan
300 schema:name Division of Host–Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan
301 Second Department of Internal Medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan
302 Second Department of Internal medicine, Wakayama Medical College, 811-1 Kimiidera, 640-0012, Wakayama, Japan
303 rdf:type schema:Organization
304 grid-institutes:grid.420033.6 schema:alternateName Laboratory Animal Research Station, Nippon Institute for Biological Science, 3331, Kamisasao, 408-0041, Kobuchizawa, Yamanashi, Japan
305 schema:name Laboratory Animal Research Station, Nippon Institute for Biological Science, 3331, Kamisasao, 408-0041, Kobuchizawa, Yamanashi, Japan
306 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...