Wild-type p53 transactivates the KILLER/DR5 gene through an intronic sequence-specific DNA-binding site View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-03

AUTHORS

R Takimoto, W S El-Deiry

ABSTRACT

KILLER/DR5, a tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor gene, has been shown to be induced by DNA damaging agents and radiation in a p53-dependent manner. Although TRAIL is a potential therapeutic agent for cancer, the induction mechanism of its receptors is poorly understood. Here we show the identification of three p53 DNA-binding sites in the KILLER/DR5 genomic locus located upstream (BS1; -0.82 Kb) of the ATG site, within Intron 1 (BS2; +0.25 Kb downstream of the ATG) and within Intron 2 (BS3; +1.25 Kb downstream of the ATG). A modified p53-binding and immunoselection protocol using a wild-type p53-expressing adenovirus vector (Ad-p53) was used to identify the binding sites and to show that each binding site can bind specifically to wild-type p53 protein (wt-p53). A reporter assay revealed that only BS2 could enhance luciferase expression driven by a basal promoter. We constructed a reporter plasmid carrying the genomic regulatory region of KILLER/DR5 including the three p53 DNA-binding sites but no additional basal promoter. The genomic fragment showed basal transcriptional activity which was induced by wt-p53 but not by mutant p53, and human papilloma virus E6 inhibited the p53-dependent activation. Mutation of BS2 abrogated not only the binding activity of wt-p53 but also the induction of the KILLER/DR5 genomic promoter-reporter gene, indicating that BS2 is responsible for the p53-dependent transactivation of KILLER/ DR5. In p53-wild-type but not -mutant or -null cell lines, doxorubicin treatment stabilized p53 protein, and increased specific binding to BS2 as revealed by EMSA, and upregulated the KILLER/DR5 promoter-luciferase reporter gene. These results suggest that the transactivation of KILLER/DR5 is directly regulated by exogenous or endogenous wt-p53 and establishes KILLER/DR5 as a p53 target gene that can signal apoptotic death. More... »

PAGES

1203489

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.onc.1203489

DOI

http://dx.doi.org/10.1038/sj.onc.1203489

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045600742

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10777207


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Damage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Doxorubicin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Introns", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oncogene Proteins, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, TNF-Related Apoptosis-Inducing Ligand", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Tumor Necrosis Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcriptional Activation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Suppressor Protein p53", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Laboratory of Molecular Oncology and Cell Cycle Regulation, Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takimoto", 
        "givenName": "R", 
        "id": "sg:person.01156250213.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156250213.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Laboratory of Molecular Oncology and Cell Cycle Regulation, Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA", 
            "Department of Medicine, Pharmacology, and Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA", 
            "Cancer Center and the Institute for Human Gene Therapy, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "El-Deiry", 
        "givenName": "W S", 
        "id": "sg:person.01320537310.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320537310.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/ng0492-45", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003183829", 
          "https://doi.org/10.1038/ng0492-45"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(06)00422-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008494464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/362857a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008690526", 
          "https://doi.org/10.1038/362857a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.7.7a.1126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009683263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/1074-7613(95)90057-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012226119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90593-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014799709"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(06)00297-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015434396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201264", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015577678", 
          "https://doi.org/10.1038/sj.onc.1201264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201264", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015577678", 
          "https://doi.org/10.1038/sj.onc.1201264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.186.7.1165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021035796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/38525", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023661637", 
          "https://doi.org/10.1038/38525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/38525", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023661637", 
          "https://doi.org/10.1038/38525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/16.17.5386", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028431262"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90412-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028439044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80400-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030443302"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(97)01231-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030656212"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80399-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030882336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1203025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032194524", 
          "https://doi.org/10.1038/sj.onc.1203025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1203025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032194524", 
          "https://doi.org/10.1038/sj.onc.1203025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.188.11.2033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034791477"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81874-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036401594"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036533215", 
          "https://doi.org/10.1038/sj.onc.1201002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036533215", 
          "https://doi.org/10.1038/sj.onc.1201002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.22.12687", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037131813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90719-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037459188"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90500-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038576682"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1097-141", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040087526", 
          "https://doi.org/10.1038/ng1097-141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.6.1998", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043164457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201755", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043632183", 
          "https://doi.org/10.1038/sj.onc.1201755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201755", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043632183", 
          "https://doi.org/10.1038/sj.onc.1201755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/scbi.1998.0097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043959165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.14.2584", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045737863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.41.25417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050349376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.277.5327.815", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062557614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.277.5327.818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062557615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.281.5381.1305", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062562275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.281.5381.1309", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062562276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7973727", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062650847"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074481980", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074595974", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082505786", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082505792", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082511555", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1994.tb06807.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082582474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082659570", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082690821", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082720611", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1993.tb05941.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082762184"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082883117", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/97231bm19", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083120421"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083246531", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083251649", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083311289", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083347707", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-03", 
    "datePublishedReg": "2000-03-01", 
    "description": "KILLER/DR5, a tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor gene, has been shown to be induced by DNA damaging agents and radiation in a p53-dependent manner. Although TRAIL is a potential therapeutic agent for cancer, the induction mechanism of its receptors is poorly understood. Here we show the identification of three p53 DNA-binding sites in the KILLER/DR5 genomic locus located upstream (BS1; -0.82 Kb) of the ATG site, within Intron 1 (BS2; +0.25 Kb downstream of the ATG) and within Intron 2 (BS3; +1.25 Kb downstream of the ATG). A modified p53-binding and immunoselection protocol using a wild-type p53-expressing adenovirus vector (Ad-p53) was used to identify the binding sites and to show that each binding site can bind specifically to wild-type p53 protein (wt-p53). A reporter assay revealed that only BS2 could enhance luciferase expression driven by a basal promoter. We constructed a reporter plasmid carrying the genomic regulatory region of KILLER/DR5 including the three p53 DNA-binding sites but no additional basal promoter. The genomic fragment showed basal transcriptional activity which was induced by wt-p53 but not by mutant p53, and human papilloma virus E6 inhibited the p53-dependent activation. Mutation of BS2 abrogated not only the binding activity of wt-p53 but also the induction of the KILLER/DR5 genomic promoter-reporter gene, indicating that BS2 is responsible for the p53-dependent transactivation of KILLER/ DR5. In p53-wild-type but not -mutant or -null cell lines, doxorubicin treatment stabilized p53 protein, and increased specific binding to BS2 as revealed by EMSA, and upregulated the KILLER/DR5 promoter-luciferase reporter gene. These results suggest that the transactivation of KILLER/DR5 is directly regulated by exogenous or endogenous wt-p53 and establishes KILLER/DR5 as a p53 target gene that can signal apoptotic death.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.onc.1203489", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2435689", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1097543", 
        "issn": [
          "0950-9232", 
          "1476-5594"
        ], 
        "name": "Oncogene", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "14", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "name": "Wild-type p53 transactivates the KILLER/DR5 gene through an intronic sequence-specific DNA-binding site", 
    "pagination": "1203489", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d93770313b192691238f4b9a98b1c24ab347c9effa2d3f3ae344a5c87f1e6abe"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10777207"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8711562"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.onc.1203489"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045600742"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.onc.1203489", 
      "https://app.dimensions.ai/details/publication/pub.1045600742"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:14", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_54002_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/1203489"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203489'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203489'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203489'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203489'


 

This table displays all metadata directly associated to this object as RDF triples.

290 TRIPLES      21 PREDICATES      95 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.onc.1203489 schema:about N324dbe828b6d472daeb77ad71497ed30
2 N35f10cef5a194d2d9d67d60f7433b0f1
3 N64a4583134a44deca2e203fac344a7a1
4 N7d0fa53c3991477e854b3034d7aed2ee
5 N84098c67257a4512a9f797bbcf88e5e7
6 N876eaf37593447ed90d718fc5170a6e1
7 N8a675758fba245519766d04ee5cc5693
8 N8e7d31ef9dfd40aab216916074c6b773
9 N96bf078e16fe4ae3ba7d07ee64fe88f0
10 N9c48bc7e0ab14445b8cc695d56055add
11 Na01245c983ad4c42b6b4df1c609b2e9b
12 Na42f9ba5c8bb4f52beaa39fae4742b92
13 Na8f67cda177f45a7958905ff8ec3f424
14 Nac35deec2c164889b0d411b90bcaac64
15 Ne30c26fefcfd493c9c9c93f69a5ab121
16 Neee696cb418f4d818fe1308f0bd5830e
17 Nff5a8b38d133460e8b3f87cb087e8a28
18 anzsrc-for:06
19 anzsrc-for:0604
20 schema:author N8b9c627f27244501924aafcf66c630e7
21 schema:citation sg:pub.10.1038/362857a0
22 sg:pub.10.1038/38525
23 sg:pub.10.1038/ng0492-45
24 sg:pub.10.1038/ng1097-141
25 sg:pub.10.1038/sj.onc.1201002
26 sg:pub.10.1038/sj.onc.1201264
27 sg:pub.10.1038/sj.onc.1201755
28 sg:pub.10.1038/sj.onc.1203025
29 https://app.dimensions.ai/details/publication/pub.1074481980
30 https://app.dimensions.ai/details/publication/pub.1074595974
31 https://app.dimensions.ai/details/publication/pub.1082505786
32 https://app.dimensions.ai/details/publication/pub.1082505792
33 https://app.dimensions.ai/details/publication/pub.1082511555
34 https://app.dimensions.ai/details/publication/pub.1082659570
35 https://app.dimensions.ai/details/publication/pub.1082690821
36 https://app.dimensions.ai/details/publication/pub.1082720611
37 https://app.dimensions.ai/details/publication/pub.1082883117
38 https://app.dimensions.ai/details/publication/pub.1083246531
39 https://app.dimensions.ai/details/publication/pub.1083251649
40 https://app.dimensions.ai/details/publication/pub.1083311289
41 https://app.dimensions.ai/details/publication/pub.1083347707
42 https://doi.org/10.1002/j.1460-2075.1993.tb05941.x
43 https://doi.org/10.1002/j.1460-2075.1994.tb06807.x
44 https://doi.org/10.1006/scbi.1998.0097
45 https://doi.org/10.1016/0092-8674(92)90593-2
46 https://doi.org/10.1016/0092-8674(93)90500-p
47 https://doi.org/10.1016/0092-8674(93)90719-7
48 https://doi.org/10.1016/0092-8674(95)90412-3
49 https://doi.org/10.1016/1074-7613(95)90057-8
50 https://doi.org/10.1016/s0014-5793(97)01231-3
51 https://doi.org/10.1016/s0092-8674(00)81874-7
52 https://doi.org/10.1016/s0960-9822(06)00297-1
53 https://doi.org/10.1016/s0960-9822(06)00422-2
54 https://doi.org/10.1016/s1074-7613(00)80399-4
55 https://doi.org/10.1016/s1074-7613(00)80400-8
56 https://doi.org/10.1073/pnas.91.6.1998
57 https://doi.org/10.1074/jbc.271.22.12687
58 https://doi.org/10.1074/jbc.272.41.25417
59 https://doi.org/10.1084/jem.186.7.1165
60 https://doi.org/10.1084/jem.188.11.2033
61 https://doi.org/10.1093/emboj/16.17.5386
62 https://doi.org/10.1093/nar/23.14.2584
63 https://doi.org/10.1101/gad.7.7a.1126
64 https://doi.org/10.1126/science.277.5327.815
65 https://doi.org/10.1126/science.277.5327.818
66 https://doi.org/10.1126/science.281.5381.1305
67 https://doi.org/10.1126/science.281.5381.1309
68 https://doi.org/10.1126/science.7973727
69 https://doi.org/10.2144/97231bm19
70 schema:datePublished 2000-03
71 schema:datePublishedReg 2000-03-01
72 schema:description KILLER/DR5, a tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor gene, has been shown to be induced by DNA damaging agents and radiation in a p53-dependent manner. Although TRAIL is a potential therapeutic agent for cancer, the induction mechanism of its receptors is poorly understood. Here we show the identification of three p53 DNA-binding sites in the KILLER/DR5 genomic locus located upstream (BS1; -0.82 Kb) of the ATG site, within Intron 1 (BS2; +0.25 Kb downstream of the ATG) and within Intron 2 (BS3; +1.25 Kb downstream of the ATG). A modified p53-binding and immunoselection protocol using a wild-type p53-expressing adenovirus vector (Ad-p53) was used to identify the binding sites and to show that each binding site can bind specifically to wild-type p53 protein (wt-p53). A reporter assay revealed that only BS2 could enhance luciferase expression driven by a basal promoter. We constructed a reporter plasmid carrying the genomic regulatory region of KILLER/DR5 including the three p53 DNA-binding sites but no additional basal promoter. The genomic fragment showed basal transcriptional activity which was induced by wt-p53 but not by mutant p53, and human papilloma virus E6 inhibited the p53-dependent activation. Mutation of BS2 abrogated not only the binding activity of wt-p53 but also the induction of the KILLER/DR5 genomic promoter-reporter gene, indicating that BS2 is responsible for the p53-dependent transactivation of KILLER/ DR5. In p53-wild-type but not -mutant or -null cell lines, doxorubicin treatment stabilized p53 protein, and increased specific binding to BS2 as revealed by EMSA, and upregulated the KILLER/DR5 promoter-luciferase reporter gene. These results suggest that the transactivation of KILLER/DR5 is directly regulated by exogenous or endogenous wt-p53 and establishes KILLER/DR5 as a p53 target gene that can signal apoptotic death.
73 schema:genre research_article
74 schema:inLanguage en
75 schema:isAccessibleForFree true
76 schema:isPartOf N0e64dfd846f54eb7a2e9c7f49358e346
77 Nf7884922b62242ad9fbcfedc76a485bf
78 sg:journal.1097543
79 schema:name Wild-type p53 transactivates the KILLER/DR5 gene through an intronic sequence-specific DNA-binding site
80 schema:pagination 1203489
81 schema:productId N2073fb03e0d2489db284d57890669a58
82 N2ea5dc4c20c645c09e0cca59ab12370d
83 N86132b532e1b4910bad6ac6c0ee6b5f6
84 N8f9d668c77f24c44ad44fc57948b828d
85 Nff6b86a4b8a54606a1ca1113b90bea1b
86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045600742
87 https://doi.org/10.1038/sj.onc.1203489
88 schema:sdDatePublished 2019-04-11T12:14
89 schema:sdLicense https://scigraph.springernature.com/explorer/license/
90 schema:sdPublisher N59354406d3874b9a8d780fbcbcd01632
91 schema:url http://www.nature.com/articles/1203489
92 sgo:license sg:explorer/license/
93 sgo:sdDataset articles
94 rdf:type schema:ScholarlyArticle
95 N0e64dfd846f54eb7a2e9c7f49358e346 schema:issueNumber 14
96 rdf:type schema:PublicationIssue
97 N2073fb03e0d2489db284d57890669a58 schema:name readcube_id
98 schema:value d93770313b192691238f4b9a98b1c24ab347c9effa2d3f3ae344a5c87f1e6abe
99 rdf:type schema:PropertyValue
100 N2ea5dc4c20c645c09e0cca59ab12370d schema:name dimensions_id
101 schema:value pub.1045600742
102 rdf:type schema:PropertyValue
103 N324dbe828b6d472daeb77ad71497ed30 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Binding Sites
105 rdf:type schema:DefinedTerm
106 N35f10cef5a194d2d9d67d60f7433b0f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Receptors, TNF-Related Apoptosis-Inducing Ligand
108 rdf:type schema:DefinedTerm
109 N59354406d3874b9a8d780fbcbcd01632 schema:name Springer Nature - SN SciGraph project
110 rdf:type schema:Organization
111 N64a4583134a44deca2e203fac344a7a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Promoter Regions, Genetic
113 rdf:type schema:DefinedTerm
114 N7d0fa53c3991477e854b3034d7aed2ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Doxorubicin
116 rdf:type schema:DefinedTerm
117 N84098c67257a4512a9f797bbcf88e5e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Genes, Reporter
119 rdf:type schema:DefinedTerm
120 N86132b532e1b4910bad6ac6c0ee6b5f6 schema:name doi
121 schema:value 10.1038/sj.onc.1203489
122 rdf:type schema:PropertyValue
123 N876eaf37593447ed90d718fc5170a6e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Transcriptional Activation
125 rdf:type schema:DefinedTerm
126 N8a675758fba245519766d04ee5cc5693 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Oncogene Proteins, Viral
128 rdf:type schema:DefinedTerm
129 N8b9c627f27244501924aafcf66c630e7 rdf:first sg:person.01156250213.33
130 rdf:rest Ncd617ad02fbb4b68a57d777267eb50ba
131 N8e7d31ef9dfd40aab216916074c6b773 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Tumor Suppressor Protein p53
133 rdf:type schema:DefinedTerm
134 N8f9d668c77f24c44ad44fc57948b828d schema:name pubmed_id
135 schema:value 10777207
136 rdf:type schema:PropertyValue
137 N96bf078e16fe4ae3ba7d07ee64fe88f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Gene Expression
139 rdf:type schema:DefinedTerm
140 N9c48bc7e0ab14445b8cc695d56055add schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Receptors, Tumor Necrosis Factor
142 rdf:type schema:DefinedTerm
143 Na01245c983ad4c42b6b4df1c609b2e9b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Tumor Cells, Cultured
145 rdf:type schema:DefinedTerm
146 Na42f9ba5c8bb4f52beaa39fae4742b92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name DNA
148 rdf:type schema:DefinedTerm
149 Na8f67cda177f45a7958905ff8ec3f424 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Mutagenesis
151 rdf:type schema:DefinedTerm
152 Nac35deec2c164889b0d411b90bcaac64 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Introns
154 rdf:type schema:DefinedTerm
155 Ncd617ad02fbb4b68a57d777267eb50ba rdf:first sg:person.01320537310.58
156 rdf:rest rdf:nil
157 Ne30c26fefcfd493c9c9c93f69a5ab121 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Apoptosis
159 rdf:type schema:DefinedTerm
160 Neee696cb418f4d818fe1308f0bd5830e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Humans
162 rdf:type schema:DefinedTerm
163 Nf7884922b62242ad9fbcfedc76a485bf schema:volumeNumber 19
164 rdf:type schema:PublicationVolume
165 Nff5a8b38d133460e8b3f87cb087e8a28 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name DNA Damage
167 rdf:type schema:DefinedTerm
168 Nff6b86a4b8a54606a1ca1113b90bea1b schema:name nlm_unique_id
169 schema:value 8711562
170 rdf:type schema:PropertyValue
171 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
172 schema:name Biological Sciences
173 rdf:type schema:DefinedTerm
174 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
175 schema:name Genetics
176 rdf:type schema:DefinedTerm
177 sg:grant.2435689 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.onc.1203489
178 rdf:type schema:MonetaryGrant
179 sg:journal.1097543 schema:issn 0950-9232
180 1476-5594
181 schema:name Oncogene
182 rdf:type schema:Periodical
183 sg:person.01156250213.33 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
184 schema:familyName Takimoto
185 schema:givenName R
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156250213.33
187 rdf:type schema:Person
188 sg:person.01320537310.58 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
189 schema:familyName El-Deiry
190 schema:givenName W S
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320537310.58
192 rdf:type schema:Person
193 sg:pub.10.1038/362857a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008690526
194 https://doi.org/10.1038/362857a0
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/38525 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023661637
197 https://doi.org/10.1038/38525
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/ng0492-45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003183829
200 https://doi.org/10.1038/ng0492-45
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/ng1097-141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040087526
203 https://doi.org/10.1038/ng1097-141
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/sj.onc.1201002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036533215
206 https://doi.org/10.1038/sj.onc.1201002
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/sj.onc.1201264 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015577678
209 https://doi.org/10.1038/sj.onc.1201264
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/sj.onc.1201755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043632183
212 https://doi.org/10.1038/sj.onc.1201755
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/sj.onc.1203025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032194524
215 https://doi.org/10.1038/sj.onc.1203025
216 rdf:type schema:CreativeWork
217 https://app.dimensions.ai/details/publication/pub.1074481980 schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1074595974 schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1082505786 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1082505792 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1082511555 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1082659570 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1082690821 schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1082720611 schema:CreativeWork
225 https://app.dimensions.ai/details/publication/pub.1082883117 schema:CreativeWork
226 https://app.dimensions.ai/details/publication/pub.1083246531 schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1083251649 schema:CreativeWork
228 https://app.dimensions.ai/details/publication/pub.1083311289 schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1083347707 schema:CreativeWork
230 https://doi.org/10.1002/j.1460-2075.1993.tb05941.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082762184
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1002/j.1460-2075.1994.tb06807.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082582474
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1006/scbi.1998.0097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043959165
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/0092-8674(92)90593-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014799709
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/0092-8674(93)90500-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1038576682
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/0092-8674(93)90719-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037459188
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/0092-8674(95)90412-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028439044
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/1074-7613(95)90057-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012226119
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s0014-5793(97)01231-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030656212
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/s0092-8674(00)81874-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036401594
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/s0960-9822(06)00297-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015434396
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s0960-9822(06)00422-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008494464
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s1074-7613(00)80399-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030882336
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/s1074-7613(00)80400-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030443302
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1073/pnas.91.6.1998 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043164457
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1074/jbc.271.22.12687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037131813
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1074/jbc.272.41.25417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050349376
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1084/jem.186.7.1165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021035796
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1084/jem.188.11.2033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034791477
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1093/emboj/16.17.5386 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028431262
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1093/nar/23.14.2584 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045737863
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1101/gad.7.7a.1126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009683263
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1126/science.277.5327.815 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062557614
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1126/science.277.5327.818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062557615
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1126/science.281.5381.1305 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062562275
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1126/science.281.5381.1309 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062562276
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1126/science.7973727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062650847
283 rdf:type schema:CreativeWork
284 https://doi.org/10.2144/97231bm19 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083120421
285 rdf:type schema:CreativeWork
286 https://www.grid.ac/institutes/grid.25879.31 schema:alternateName University of Pennsylvania
287 schema:name Cancer Center and the Institute for Human Gene Therapy, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA
288 Department of Medicine, Pharmacology, and Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA
289 Laboratory of Molecular Oncology and Cell Cycle Regulation, Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA
290 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...