Localization of membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 at tight junctions of epithelial cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-12

AUTHORS

Nobuyuki Ide, Yutake Hata, Hideo Nishioka, Kazuyo Hirao, Ikuko Yao, Maki Deguchi, Akira Mizoguchi, Hiroyuki Nishimori, Takashi Tokino, Yusuke Nakamura, Yoshimi Takai

ABSTRACT

Membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 and Synapse-associated protein (SAP) 97/human Discs-large tumor suppressor gene (hDLG) are ubiquitous isoforms of synaptic scaffolding molecule (S-SCAM) and Postsynaptic density (PSD)-95/SAP90, both of which are implicated in the structures of synapses, respectively. SAP97/hDLG is localized at epithelial junctions and may function as a scaffolding protein, but the subcellular localization or the function of MAGI-1/BAP1 has not been clarified. In intestinal epithelial cells, MAGI-1/BAP1 was localized at tight junctions, whereas SAP97/hDLG was localized diffusely at cell – cell junctions. In Madine Darby canine kidney (MDCK) cells, MAGI-1/BAP1 was colocalized with ZO-1, whereas SAP97/hDLG was colocalized with E-cadherin. In MDCK cells, dominant active and negative mutants of Rac1 small G protein changed the amounts of SAP97/hDLG at cell – cell junctions, but not that of MAGI-1/BAP1. When MDCK cells were switched to a low Ca2+ medium, E-cadherin disappeared from the plasma membrane, and cells were dissociated. The phorbol 12-myristate 13-acetate-treatment after the low Ca2+ switch induced a tight junction-like structure. MAGI-1/BAP1 was recruited with ZO-1 to this structure, but SAP97/hDLG or E-cadherin was not. These findings suggest that MAGI-1/BAP1 is a component of tight junctions of epithelial cells, and that its role is different from that of SAP97/hDLG. More... »

PAGES

7810-7815

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.onc.1203153

DOI

http://dx.doi.org/10.1038/sj.onc.1203153

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035985222

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10618722


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adaptor Proteins, Signal Transducing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Angiogenesis Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Monoclonal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "COS Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion Molecules", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion Molecules, Neuronal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dogs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelial Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guanylate Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunohistochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intestinal Mucosa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intestine, Small", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Immunoelectron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleoside-Phosphate Kinase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tetradecanoylphorbol Acetate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tight Junctions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zonula Occludens-1 Protein", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ide", 
        "givenName": "Nobuyuki", 
        "id": "sg:person.01162346441.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162346441.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hata", 
        "givenName": "Yutake", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nishioka", 
        "givenName": "Hideo", 
        "id": "sg:person.0672617265.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672617265.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hirao", 
        "givenName": "Kazuyo", 
        "id": "sg:person.0674565374.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674565374.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yao", 
        "givenName": "Ikuko", 
        "id": "sg:person.01136115773.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136115773.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Deguchi", 
        "givenName": "Maki", 
        "id": "sg:person.01302436141.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302436141.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Anatomy and Neurobiology, Graduate School, Kyoto University, 606-8315, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Anatomy and Neurobiology, Graduate School, Kyoto University, 606-8315, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mizoguchi", 
        "givenName": "Akira", 
        "id": "sg:person.01372323174.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372323174.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nishimori", 
        "givenName": "Hiroyuki", 
        "id": "sg:person.01314027530.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314027530.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tokino", 
        "givenName": "Takashi", 
        "id": "sg:person.01272777202.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272777202.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakamura", 
        "givenName": "Yusuke", 
        "id": "sg:person.011310672653.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011310672653.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, 565-0871, Suita, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan", 
            "Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, 565-0871, Suita, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takai", 
        "givenName": "Yoshimi", 
        "id": "sg:person.0770305732.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770305732.70"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/sj.onc.1202182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001000305", 
          "https://doi.org/10.1038/sj.onc.1202182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1202652", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011277311", 
          "https://doi.org/10.1038/sj.onc.1202652"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-12", 
    "datePublishedReg": "1999-12-01", 
    "description": "Membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 and Synapse-associated protein (SAP) 97/human Discs-large tumor suppressor gene (hDLG) are ubiquitous isoforms of synaptic scaffolding molecule (S-SCAM) and Postsynaptic density (PSD)-95/SAP90, both of which are implicated in the structures of synapses, respectively. SAP97/hDLG is localized at epithelial junctions and may function as a scaffolding protein, but the subcellular localization or the function of MAGI-1/BAP1 has not been clarified. In intestinal epithelial cells, MAGI-1/BAP1 was localized at tight junctions, whereas SAP97/hDLG was localized diffusely at cell\u2009\u2013\u2009cell junctions. In Madine Darby canine kidney (MDCK) cells, MAGI-1/BAP1 was colocalized with ZO-1, whereas SAP97/hDLG was colocalized with E-cadherin. In MDCK cells, dominant active and negative mutants of Rac1 small G protein changed the amounts of SAP97/hDLG at cell\u2009\u2013\u2009cell junctions, but not that of MAGI-1/BAP1. When MDCK cells were switched to a low Ca2+ medium, E-cadherin disappeared from the plasma membrane, and cells were dissociated. The phorbol 12-myristate 13-acetate-treatment after the low Ca2+ switch induced a tight junction-like structure. MAGI-1/BAP1 was recruited with ZO-1 to this structure, but SAP97/hDLG or E-cadherin was not. These findings suggest that MAGI-1/BAP1 is a component of tight junctions of epithelial cells, and that its role is different from that of SAP97/hDLG.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/sj.onc.1203153", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1097543", 
        "issn": [
          "0950-9232", 
          "1476-5594"
        ], 
        "name": "Oncogene", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "54", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "keywords": [
      "guanylate kinase", 
      "membrane-associated guanylate kinase", 
      "cell junctions", 
      "MDCK cells", 
      "epithelial cells", 
      "protein 1", 
      "tight junctions", 
      "ZO-1", 
      "tumor suppressor gene", 
      "Madine-Darby canine kidney (MDCK) cells", 
      "canine kidney cells", 
      "subcellular localization", 
      "negative mutant", 
      "plasma membrane", 
      "ubiquitous isoform", 
      "junction-like structures", 
      "intestinal epithelial cells", 
      "tight junction-like structures", 
      "suppressor gene", 
      "epithelial junctions", 
      "Darby canine kidney cells", 
      "cadherin", 
      "kidney cells", 
      "postsynaptic density", 
      "BAP1", 
      "kinase", 
      "structure of synapses", 
      "protein", 
      "cells", 
      "low Ca2", 
      "membrane", 
      "mutants", 
      "SAP90", 
      "localization", 
      "Rac1", 
      "genes", 
      "Ca2", 
      "isoforms", 
      "phorbol", 
      "junction", 
      "synapse", 
      "molecules", 
      "structure", 
      "synapses", 
      "role", 
      "switch", 
      "function", 
      "components", 
      "medium", 
      "amount", 
      "findings", 
      "Bai", 
      "density", 
      "protein (SAP) 97/human Discs-large tumor suppressor gene", 
      "hDLG", 
      "Discs-large tumor suppressor gene", 
      "SAP97/hDLG", 
      "MAGI-1/BAP1"
    ], 
    "name": "Localization of membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 at tight junctions of epithelial cells", 
    "pagination": "7810-7815", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1035985222"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.onc.1203153"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10618722"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.onc.1203153", 
      "https://app.dimensions.ai/details/publication/pub.1035985222"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:11", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_344.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/sj.onc.1203153"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203153'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203153'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203153'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1203153'


 

This table displays all metadata directly associated to this object as RDF triples.

315 TRIPLES      22 PREDICATES      114 URIs      103 LITERALS      33 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.onc.1203153 schema:about N0e8046a0e8024b18ab8547d7a40355d2
2 N16fc03967b154d119264612a67acfd0f
3 N185937446ae64249b50c210b6bc8d3bb
4 N18eca20eb98941e5b779325946234abd
5 N1b348dbf90204f5db6e23a0eb4a61171
6 N2a860b6c607e44c8b1a0d23f27cafcea
7 N2db686b3ff0a44f48b77e23b89a6ced8
8 N3f17ecb6aeb74638bfe33894fc680bc7
9 N3f4244d933ca43ddbfe8148b58b02db8
10 N44d9c51cb975497fa8ebc4ab218368fd
11 N58284a6032a64b33a224e373e0ebc911
12 N6931ba4f9dfe42f3aae5a1048b44e919
13 N7014c48171e240a1b75c2a31b5e83287
14 N716bc30450954870bd07a0b7dbe301b8
15 N7ad3be77533747e6b337b45639ebe53d
16 N86d7ef11bdcd4130beb3b05a21adcf74
17 N91e2b09422d243d5ac66c82226607a33
18 N9b091649d45c4aa5a935ad5b592efc7b
19 Nb8940db9d7c54a0ea94c99263320c91e
20 Ncffb56b8352040d4b41d4cabf53f850d
21 Ndcefafcfd80a4a259219af1deefb6599
22 Ndfd6eb3f91c243ff9d5cfea163e231d7
23 Ne941008526c94249aa6055f48386ca7d
24 Ned64ef84e4e84341a212fff51c47f874
25 Neddac973faad42ef910280b800d8d98c
26 Nff064a009c704d2a8e9574bcf8aa3319
27 anzsrc-for:11
28 anzsrc-for:1103
29 anzsrc-for:1112
30 schema:author N15b59816f1f1485886c62c8491163c12
31 schema:citation sg:pub.10.1038/sj.onc.1202182
32 sg:pub.10.1038/sj.onc.1202652
33 schema:datePublished 1999-12
34 schema:datePublishedReg 1999-12-01
35 schema:description Membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 and Synapse-associated protein (SAP) 97/human Discs-large tumor suppressor gene (hDLG) are ubiquitous isoforms of synaptic scaffolding molecule (S-SCAM) and Postsynaptic density (PSD)-95/SAP90, both of which are implicated in the structures of synapses, respectively. SAP97/hDLG is localized at epithelial junctions and may function as a scaffolding protein, but the subcellular localization or the function of MAGI-1/BAP1 has not been clarified. In intestinal epithelial cells, MAGI-1/BAP1 was localized at tight junctions, whereas SAP97/hDLG was localized diffusely at cell – cell junctions. In Madine Darby canine kidney (MDCK) cells, MAGI-1/BAP1 was colocalized with ZO-1, whereas SAP97/hDLG was colocalized with E-cadherin. In MDCK cells, dominant active and negative mutants of Rac1 small G protein changed the amounts of SAP97/hDLG at cell – cell junctions, but not that of MAGI-1/BAP1. When MDCK cells were switched to a low Ca2+ medium, E-cadherin disappeared from the plasma membrane, and cells were dissociated. The phorbol 12-myristate 13-acetate-treatment after the low Ca2+ switch induced a tight junction-like structure. MAGI-1/BAP1 was recruited with ZO-1 to this structure, but SAP97/hDLG or E-cadherin was not. These findings suggest that MAGI-1/BAP1 is a component of tight junctions of epithelial cells, and that its role is different from that of SAP97/hDLG.
36 schema:genre article
37 schema:inLanguage en
38 schema:isAccessibleForFree true
39 schema:isPartOf Nd4dc0667ce494f6daa79295aa522a050
40 Neb58f7ac49f94405a4967368c0ead063
41 sg:journal.1097543
42 schema:keywords BAP1
43 Bai
44 Ca2
45 Darby canine kidney cells
46 Discs-large tumor suppressor gene
47 MAGI-1/BAP1
48 MDCK cells
49 Madine-Darby canine kidney (MDCK) cells
50 Rac1
51 SAP90
52 SAP97/hDLG
53 ZO-1
54 amount
55 cadherin
56 canine kidney cells
57 cell junctions
58 cells
59 components
60 density
61 epithelial cells
62 epithelial junctions
63 findings
64 function
65 genes
66 guanylate kinase
67 hDLG
68 intestinal epithelial cells
69 isoforms
70 junction
71 junction-like structures
72 kidney cells
73 kinase
74 localization
75 low Ca2
76 medium
77 membrane
78 membrane-associated guanylate kinase
79 molecules
80 mutants
81 negative mutant
82 phorbol
83 plasma membrane
84 postsynaptic density
85 protein
86 protein (SAP) 97/human Discs-large tumor suppressor gene
87 protein 1
88 role
89 structure
90 structure of synapses
91 subcellular localization
92 suppressor gene
93 switch
94 synapse
95 synapses
96 tight junction-like structures
97 tight junctions
98 tumor suppressor gene
99 ubiquitous isoform
100 schema:name Localization of membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 at tight junctions of epithelial cells
101 schema:pagination 7810-7815
102 schema:productId N1ce9d1023c414221bbe74c4312bae81d
103 N28f29e266e9442e0a8e621639fd5a109
104 Nbef148a2a1324319a95173eb3e48b662
105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035985222
106 https://doi.org/10.1038/sj.onc.1203153
107 schema:sdDatePublished 2022-01-01T18:11
108 schema:sdLicense https://scigraph.springernature.com/explorer/license/
109 schema:sdPublisher N198ddcef66c643d3b93eb0ba6b8247e6
110 schema:url https://doi.org/10.1038/sj.onc.1203153
111 sgo:license sg:explorer/license/
112 sgo:sdDataset articles
113 rdf:type schema:ScholarlyArticle
114 N09a40dc7ee47402382102d86bd05b4fb rdf:first sg:person.0672617265.03
115 rdf:rest N7e083546aba747b58ea18841fe39e980
116 N0ca399def5fc43e58ae6a6415eb55b0d rdf:first sg:person.01302436141.10
117 rdf:rest N3e1623b212194b77a2ebd9a4b5aa7e5c
118 N0e8046a0e8024b18ab8547d7a40355d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Microscopy, Immunoelectron
120 rdf:type schema:DefinedTerm
121 N15b59816f1f1485886c62c8491163c12 rdf:first sg:person.01162346441.62
122 rdf:rest N1fa8a3a73e1048de90bf1d73c2fab73c
123 N16fc03967b154d119264612a67acfd0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Animals
125 rdf:type schema:DefinedTerm
126 N185937446ae64249b50c210b6bc8d3bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Humans
128 rdf:type schema:DefinedTerm
129 N18eca20eb98941e5b779325946234abd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Kidney
131 rdf:type schema:DefinedTerm
132 N198ddcef66c643d3b93eb0ba6b8247e6 schema:name Springer Nature - SN SciGraph project
133 rdf:type schema:Organization
134 N1b348dbf90204f5db6e23a0eb4a61171 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Epithelial Cells
136 rdf:type schema:DefinedTerm
137 N1ce9d1023c414221bbe74c4312bae81d schema:name dimensions_id
138 schema:value pub.1035985222
139 rdf:type schema:PropertyValue
140 N1fa8a3a73e1048de90bf1d73c2fab73c rdf:first N38fe57cb2f4346ebb1505a8026513596
141 rdf:rest N09a40dc7ee47402382102d86bd05b4fb
142 N28f29e266e9442e0a8e621639fd5a109 schema:name doi
143 schema:value 10.1038/sj.onc.1203153
144 rdf:type schema:PropertyValue
145 N2a860b6c607e44c8b1a0d23f27cafcea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Cell Line
147 rdf:type schema:DefinedTerm
148 N2db686b3ff0a44f48b77e23b89a6ced8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Recombinant Proteins
150 rdf:type schema:DefinedTerm
151 N38fe57cb2f4346ebb1505a8026513596 schema:affiliation grid-institutes:None
152 schema:familyName Hata
153 schema:givenName Yutake
154 rdf:type schema:Person
155 N3e1623b212194b77a2ebd9a4b5aa7e5c rdf:first sg:person.01372323174.47
156 rdf:rest Nf98862ff323e4aa5b2d563b69cdc469d
157 N3f17ecb6aeb74638bfe33894fc680bc7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Intestine, Small
159 rdf:type schema:DefinedTerm
160 N3f4244d933ca43ddbfe8148b58b02db8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Phosphoproteins
162 rdf:type schema:DefinedTerm
163 N44d9c51cb975497fa8ebc4ab218368fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Cell Adhesion Molecules, Neuronal
165 rdf:type schema:DefinedTerm
166 N58284a6032a64b33a224e373e0ebc911 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Tetradecanoylphorbol Acetate
168 rdf:type schema:DefinedTerm
169 N6931ba4f9dfe42f3aae5a1048b44e919 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Transfection
171 rdf:type schema:DefinedTerm
172 N7014c48171e240a1b75c2a31b5e83287 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Angiogenesis Inhibitors
174 rdf:type schema:DefinedTerm
175 N716bc30450954870bd07a0b7dbe301b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Cell Adhesion Molecules
177 rdf:type schema:DefinedTerm
178 N722ef6f6522c4e7796e311104e451cec rdf:first sg:person.01272777202.67
179 rdf:rest Ne82b1ac38de74242837231238562714b
180 N7ad3be77533747e6b337b45639ebe53d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Guanylate Kinases
182 rdf:type schema:DefinedTerm
183 N7c506b00961640f09b67cc2184c80c37 rdf:first sg:person.01136115773.14
184 rdf:rest N0ca399def5fc43e58ae6a6415eb55b0d
185 N7e083546aba747b58ea18841fe39e980 rdf:first sg:person.0674565374.08
186 rdf:rest N7c506b00961640f09b67cc2184c80c37
187 N86d7ef11bdcd4130beb3b05a21adcf74 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name Tight Junctions
189 rdf:type schema:DefinedTerm
190 N91e2b09422d243d5ac66c82226607a33 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Membrane Proteins
192 rdf:type schema:DefinedTerm
193 N9b091649d45c4aa5a935ad5b592efc7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Immunohistochemistry
195 rdf:type schema:DefinedTerm
196 Nb8940db9d7c54a0ea94c99263320c91e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
197 schema:name Intestinal Mucosa
198 rdf:type schema:DefinedTerm
199 Nbef148a2a1324319a95173eb3e48b662 schema:name pubmed_id
200 schema:value 10618722
201 rdf:type schema:PropertyValue
202 Ncffb56b8352040d4b41d4cabf53f850d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
203 schema:name Rats
204 rdf:type schema:DefinedTerm
205 Nd4dc0667ce494f6daa79295aa522a050 schema:issueNumber 54
206 rdf:type schema:PublicationIssue
207 Ndcefafcfd80a4a259219af1deefb6599 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
208 schema:name Dogs
209 rdf:type schema:DefinedTerm
210 Ndfd6eb3f91c243ff9d5cfea163e231d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
211 schema:name Adaptor Proteins, Signal Transducing
212 rdf:type schema:DefinedTerm
213 Ne82b1ac38de74242837231238562714b rdf:first sg:person.011310672653.44
214 rdf:rest Nedced1003b0443abacb981bf3598d2ff
215 Ne941008526c94249aa6055f48386ca7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
216 schema:name Nucleoside-Phosphate Kinase
217 rdf:type schema:DefinedTerm
218 Neb58f7ac49f94405a4967368c0ead063 schema:volumeNumber 18
219 rdf:type schema:PublicationVolume
220 Ned64ef84e4e84341a212fff51c47f874 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
221 schema:name Zonula Occludens-1 Protein
222 rdf:type schema:DefinedTerm
223 Nedced1003b0443abacb981bf3598d2ff rdf:first sg:person.0770305732.70
224 rdf:rest rdf:nil
225 Neddac973faad42ef910280b800d8d98c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
226 schema:name Antibodies, Monoclonal
227 rdf:type schema:DefinedTerm
228 Nf98862ff323e4aa5b2d563b69cdc469d rdf:first sg:person.01314027530.79
229 rdf:rest N722ef6f6522c4e7796e311104e451cec
230 Nff064a009c704d2a8e9574bcf8aa3319 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
231 schema:name COS Cells
232 rdf:type schema:DefinedTerm
233 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
234 schema:name Medical and Health Sciences
235 rdf:type schema:DefinedTerm
236 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
237 schema:name Clinical Sciences
238 rdf:type schema:DefinedTerm
239 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
240 schema:name Oncology and Carcinogenesis
241 rdf:type schema:DefinedTerm
242 sg:journal.1097543 schema:issn 0950-9232
243 1476-5594
244 schema:name Oncogene
245 schema:publisher Springer Nature
246 rdf:type schema:Periodical
247 sg:person.011310672653.44 schema:affiliation grid-institutes:grid.26999.3d
248 schema:familyName Nakamura
249 schema:givenName Yusuke
250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011310672653.44
251 rdf:type schema:Person
252 sg:person.01136115773.14 schema:affiliation grid-institutes:None
253 schema:familyName Yao
254 schema:givenName Ikuko
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136115773.14
256 rdf:type schema:Person
257 sg:person.01162346441.62 schema:affiliation grid-institutes:None
258 schema:familyName Ide
259 schema:givenName Nobuyuki
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162346441.62
261 rdf:type schema:Person
262 sg:person.01272777202.67 schema:affiliation grid-institutes:grid.26999.3d
263 schema:familyName Tokino
264 schema:givenName Takashi
265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272777202.67
266 rdf:type schema:Person
267 sg:person.01302436141.10 schema:affiliation grid-institutes:None
268 schema:familyName Deguchi
269 schema:givenName Maki
270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302436141.10
271 rdf:type schema:Person
272 sg:person.01314027530.79 schema:affiliation grid-institutes:grid.26999.3d
273 schema:familyName Nishimori
274 schema:givenName Hiroyuki
275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314027530.79
276 rdf:type schema:Person
277 sg:person.01372323174.47 schema:affiliation grid-institutes:grid.258799.8
278 schema:familyName Mizoguchi
279 schema:givenName Akira
280 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372323174.47
281 rdf:type schema:Person
282 sg:person.0672617265.03 schema:affiliation grid-institutes:None
283 schema:familyName Nishioka
284 schema:givenName Hideo
285 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672617265.03
286 rdf:type schema:Person
287 sg:person.0674565374.08 schema:affiliation grid-institutes:None
288 schema:familyName Hirao
289 schema:givenName Kazuyo
290 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674565374.08
291 rdf:type schema:Person
292 sg:person.0770305732.70 schema:affiliation grid-institutes:grid.136593.b
293 schema:familyName Takai
294 schema:givenName Yoshimi
295 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770305732.70
296 rdf:type schema:Person
297 sg:pub.10.1038/sj.onc.1202182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001000305
298 https://doi.org/10.1038/sj.onc.1202182
299 rdf:type schema:CreativeWork
300 sg:pub.10.1038/sj.onc.1202652 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011277311
301 https://doi.org/10.1038/sj.onc.1202652
302 rdf:type schema:CreativeWork
303 grid-institutes:None schema:alternateName Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan
304 schema:name Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan
305 rdf:type schema:Organization
306 grid-institutes:grid.136593.b schema:alternateName Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, 565-0871, Suita, Japan
307 schema:name Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, 565-0871, Suita, Japan
308 Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 651-2241, Kobe, Japan
309 rdf:type schema:Organization
310 grid-institutes:grid.258799.8 schema:alternateName Department of Anatomy and Neurobiology, Graduate School, Kyoto University, 606-8315, Kyoto, Japan
311 schema:name Department of Anatomy and Neurobiology, Graduate School, Kyoto University, 606-8315, Kyoto, Japan
312 rdf:type schema:Organization
313 grid-institutes:grid.26999.3d schema:alternateName Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan
314 schema:name Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, 108-8639, Tokyo, Japan
315 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...