Localization of membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 at tight junctions of epithelial cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-12

AUTHORS

Nobuyuki Ide, Yutake Hata, Hideo Nishioka, Kazuyo Hirao, Ikuko Yao, Maki Deguchi, Akira Mizoguchi, Hiroyuki Nishimori, Takashi Tokino, Yusuke Nakamura, Yoshimi Takai

ABSTRACT

Membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 and Synapse-associated protein (SAP) 97/human Discs-large tumor suppressor gene (hDLG) are ubiquitous isoforms of synaptic scaffolding molecule (S-SCAM) and Postsynaptic density (PSD)-95/SAP90, both of which are implicated in the structures of synapses, respectively. SAP97/hDLG is localized at epithelial junctions and may function as a scaffolding protein, but the subcellular localization or the function of MAGI-1/BAP1 has not been clarified. In intestinal epithelial cells, MAGI-1/BAP1 was localized at tight junctions, whereas SAP97/hDLG was localized diffusely at cell – cell junctions. In Madine Darby canine kidney (MDCK) cells, MAGI-1/BAP1 was colocalized with ZO-1, whereas SAP97/hDLG was colocalized with E-cadherin. In MDCK cells, dominant active and negative mutants of Rac1 small G protein changed the amounts of SAP97/hDLG at cell – cell junctions, but not that of MAGI-1/BAP1. When MDCK cells were switched to a low Ca2+ medium, E-cadherin disappeared from the plasma membrane, and cells were dissociated. The phorbol 12-myristate 13-acetate-treatment after the low Ca2+ switch induced a tight junction-like structure. MAGI-1/BAP1 was recruited with ZO-1 to this structure, but SAP97/hDLG or E-cadherin was not. These findings suggest that MAGI-1/BAP1 is a component of tight junctions of epithelial cells, and that its role is different from that of SAP97/hDLG. More... »

PAGES

7810-7815

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.onc.1203153

DOI

http://dx.doi.org/10.1038/sj.onc.1203153

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035985222

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10618722


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35 schema:description Membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 and Synapse-associated protein (SAP) 97/human Discs-large tumor suppressor gene (hDLG) are ubiquitous isoforms of synaptic scaffolding molecule (S-SCAM) and Postsynaptic density (PSD)-95/SAP90, both of which are implicated in the structures of synapses, respectively. SAP97/hDLG is localized at epithelial junctions and may function as a scaffolding protein, but the subcellular localization or the function of MAGI-1/BAP1 has not been clarified. In intestinal epithelial cells, MAGI-1/BAP1 was localized at tight junctions, whereas SAP97/hDLG was localized diffusely at cell – cell junctions. In Madine Darby canine kidney (MDCK) cells, MAGI-1/BAP1 was colocalized with ZO-1, whereas SAP97/hDLG was colocalized with E-cadherin. In MDCK cells, dominant active and negative mutants of Rac1 small G protein changed the amounts of SAP97/hDLG at cell – cell junctions, but not that of MAGI-1/BAP1. When MDCK cells were switched to a low Ca2+ medium, E-cadherin disappeared from the plasma membrane, and cells were dissociated. The phorbol 12-myristate 13-acetate-treatment after the low Ca2+ switch induced a tight junction-like structure. MAGI-1/BAP1 was recruited with ZO-1 to this structure, but SAP97/hDLG or E-cadherin was not. These findings suggest that MAGI-1/BAP1 is a component of tight junctions of epithelial cells, and that its role is different from that of SAP97/hDLG.
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43 Bai
44 Ca2
45 Darby canine kidney (MDCK) cells
46 Discs-large tumor suppressor gene
47 MAGI-1/BAP1
48 MDCK cells
49 Madine Darby canine kidney (MDCK) cells
50 Rac1
51 SAP90
52 SAP97/hDLG
53 ZO-1
54 amount
55 cadherin
56 canine kidney cells
57 cell junctions
58 cells
59 components
60 density
61 epithelial cells
62 epithelial junctions
63 findings
64 function
65 genes
66 guanylate kinase
67 hDLG
68 intestinal epithelial cells
69 isoforms
70 junction-like structures
71 junctions
72 kidney cells
73 kinase
74 localization
75 low Ca2
76 medium
77 membrane
78 membrane-associated guanylate kinase
79 molecules
80 mutants
81 negative mutant
82 phorbol 12-myristate 13-acetate-treatment
83 plasma membrane
84 postsynaptic density
85 protein
86 protein (SAP) 97/human Discs-large tumor suppressor gene
87 protein 1
88 role
89 structure
90 structure of synapses
91 subcellular localization
92 suppressor gene
93 switch
94 synapse
95 synapses
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