Ontology type: schema:ScholarlyArticle Open Access: True
1999-09
AUTHORS ABSTRACTFollowing exposure to DNA damaging agents the p53 tumour-suppressor gene induces either a growth arrest (primarily in G1) or apoptosis. The factors governing which response a cell undertakes, however, are unclear. We find that the osteosarcoma cell line, U2OS, (wild-type for p53) is capable of undergoing either p53 dependent apoptosis or cell cycle arrest in response to distinct forms of radiation. Following exposure to UVC, the majority of U2OS cells were apoptotic within 2 days and cells continued to cycle even as viability was being lost. In contrast, after X-ray treatment, U2OS cells exhibited a cell cycle arrest. Western analysis showed that p53 protein was stabilized to a greater extent by UVC than X-ray. Treatment with X-rays induced p21WAF1/CIP1 whereas p21WAF1/CIP1 expression was specifically repressed at the post-transcriptional level after exposure to UVC. Ectopic expression of high levels of p21WAF1/CIP1, which arrested U2OS cells in G1 and G2, initially conferred considerable protection against UVC-induced apoptosis. Ultimately, however, cells underwent apoptosis indicating that a high level of p21WAF1/CIP1 delays but does not block apoptosis. Taken together, these results show that cell cycle arrest and apoptosis can occur in the same cell type in response to different forms of radiation and that the repression of p21WAF1/CIP1 after UVC may contribute to the efficient induction of apoptosis in response to this particular insult. More... »
PAGES5403
http://scigraph.springernature.com/pub.10.1038/sj.onc.1202931
DOIhttp://dx.doi.org/10.1038/sj.onc.1202931
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1040228948
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/10498894
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Apoptosis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Cycle",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Division",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cyclin-Dependent Kinase Inhibitor p21",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cyclins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Regulation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transcriptional Activation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tumor Cells, Cultured",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tumor Suppressor Protein p53",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ultraviolet Rays",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "X-Rays",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Cancer Research UK",
"id": "https://www.grid.ac/institutes/grid.11485.39",
"name": [
"Eukaryotic Gene Organisation and Expression Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX"
],
"type": "Organization"
},
"familyName": "Allan",
"givenName": "Lindsey A",
"id": "sg:person.01330354334.10",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330354334.10"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Cancer Research UK",
"id": "https://www.grid.ac/institutes/grid.11485.39",
"name": [
"Eukaryotic Gene Organisation and Expression Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX"
],
"type": "Organization"
},
"familyName": "Fried",
"givenName": "Mike",
"id": "sg:person.0700011634.40",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700011634.40"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/362849a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000557273",
"https://doi.org/10.1038/362849a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1200835",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000795335",
"https://doi.org/10.1038/sj.onc.1200835"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1200835",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000795335",
"https://doi.org/10.1038/sj.onc.1200835"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.91.9.3602",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001514552"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/370220a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001574750",
"https://doi.org/10.1038/370220a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201612",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003399336",
"https://doi.org/10.1038/sj.onc.1201612"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201612",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003399336",
"https://doi.org/10.1038/sj.onc.1201612"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.9.5.600",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005353697"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1097-2765(00)80055-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006657385"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1200897",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009568327",
"https://doi.org/10.1038/sj.onc.1200897"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1200897",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009568327",
"https://doi.org/10.1038/sj.onc.1200897"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.7.7a.1126",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009683263"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/358015a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009981491",
"https://doi.org/10.1038/358015a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(84)90381-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010023016"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(93)90496-d",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011744599"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201112",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012007886",
"https://doi.org/10.1038/sj.onc.1201112"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201112",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012007886",
"https://doi.org/10.1038/sj.onc.1201112"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.95.6.2834",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014535243"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(92)90593-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014799709"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.89.16.7491",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015184889"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.16.4.1335",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017250095"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/366701a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020218634",
"https://doi.org/10.1038/366701a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.95.11.6399",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021017422"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/mc.2940140306",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021217215"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.4.9.1689",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024020108"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201899",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024298808",
"https://doi.org/10.1038/sj.onc.1201899"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201899",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024298808",
"https://doi.org/10.1038/sj.onc.1201899"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.16.9.4961",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025041194"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/371072a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026622134",
"https://doi.org/10.1038/371072a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.9.17.2170",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027465404"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/emboj/16.6.1391",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028113093"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(95)90412-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028439044"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.10.19.2438",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028611174"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.17.1.355",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030014389"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/362847a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032575717",
"https://doi.org/10.1038/362847a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.91.23.10918",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034341565"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(93)90499-g",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034836400"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.8.23.2817",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034876079"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(93)90500-p",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038576682"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.9.17.2184",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039966261"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.90.24.11623",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041670639"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0074-7696(08)62312-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042410192"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.270.30.18165",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043778174"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.16.9.4952",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044020878"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201963",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045511118",
"https://doi.org/10.1038/sj.onc.1201963"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201963",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045511118",
"https://doi.org/10.1038/sj.onc.1201963"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/377646a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046371025",
"https://doi.org/10.1038/377646a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201126",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046398743",
"https://doi.org/10.1038/sj.onc.1201126"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1201126",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046398743",
"https://doi.org/10.1038/sj.onc.1201126"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.91.18.8502",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047035821"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1083/jcb.119.3.493",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049060444"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/377552a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050160413",
"https://doi.org/10.1038/377552a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(95)90039-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051937826"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.10.15.1945",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052829675"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.10.11.5772",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053488905"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1077881311",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082424586",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082488745",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082505777",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082588280",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082659570",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1994.tb06390.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1082668473"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082690820",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082690821",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1993.tb05678.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1082807455"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1996.tb00504.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1082879603"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082901019",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082919465",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1996.tb00739.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1082941436"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082942480",
"type": "CreativeWork"
}
],
"datePublished": "1999-09",
"datePublishedReg": "1999-09-01",
"description": "Following exposure to DNA damaging agents the p53 tumour-suppressor gene induces either a growth arrest (primarily in G1) or apoptosis. The factors governing which response a cell undertakes, however, are unclear. We find that the osteosarcoma cell line, U2OS, (wild-type for p53) is capable of undergoing either p53 dependent apoptosis or cell cycle arrest in response to distinct forms of radiation. Following exposure to UVC, the majority of U2OS cells were apoptotic within 2 days and cells continued to cycle even as viability was being lost. In contrast, after X-ray treatment, U2OS cells exhibited a cell cycle arrest. Western analysis showed that p53 protein was stabilized to a greater extent by UVC than X-ray. Treatment with X-rays induced p21WAF1/CIP1 whereas p21WAF1/CIP1 expression was specifically repressed at the post-transcriptional level after exposure to UVC. Ectopic expression of high levels of p21WAF1/CIP1, which arrested U2OS cells in G1 and G2, initially conferred considerable protection against UVC-induced apoptosis. Ultimately, however, cells underwent apoptosis indicating that a high level of p21WAF1/CIP1 delays but does not block apoptosis. Taken together, these results show that cell cycle arrest and apoptosis can occur in the same cell type in response to different forms of radiation and that the repression of p21WAF1/CIP1 after UVC may contribute to the efficient induction of apoptosis in response to this particular insult.",
"genre": "research_article",
"id": "sg:pub.10.1038/sj.onc.1202931",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1097543",
"issn": [
"0950-9232",
"1476-5594"
],
"name": "Oncogene",
"type": "Periodical"
},
{
"issueNumber": "39",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "18"
}
],
"name": "p53-dependent apoptosis or growth arrest induced by different forms of radiation in U2OS cells: p21WAF1/CIP1 repression in UV induced apoptosis",
"pagination": "5403",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"bfa2bca6faa086443520bb9f09dfd1e238c78b907bd0f8427fd08112f3b2ca52"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"10498894"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"8711562"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/sj.onc.1202931"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1040228948"
]
}
],
"sameAs": [
"https://doi.org/10.1038/sj.onc.1202931",
"https://app.dimensions.ai/details/publication/pub.1040228948"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T12:06",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000360_0000000360/records_118335_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/1202931"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202931'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202931'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202931'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202931'
This table displays all metadata directly associated to this object as RDF triples.
321 TRIPLES
21 PREDICATES
105 URIs
34 LITERALS
22 BLANK NODES