Hypermethylation of the cell cycle inhibitor p15INK4b 3′-untranslated region interferes with its transcriptional regulation in primary lymphomas View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-01-20

AUTHORS

Marcos Malumbres, Ignacio Pérez de Castro, Javier Santos, José Fernández Piqueras, Angel Pellicer

ABSTRACT

The cyclin-dependent kinase inhibitor p15INK4b has been shown to be involved in human and rodent tumors and seems to act as a tumor suppressor gene in hematological malignancies. Alterations of this gene in tumors include mainly homozygous deletions and hypermethylation of the CpG island in the promoter region. In this work, we describe a new area sensitive to methylation in the 3′ untranslated region (UTR) of the murine p15INK4b gene. This region shows different levels of methylation depending on the tissues, being relatively highly methylated in brain and gut, and weakly methylated in liver, spleen or thymus. DNA methylation and expression is similar in both maternal and paternal alleles indicating no imprinting effect. Although methylation of the p15INK4b 3′-UTR is low in normal thymus, increased levels (up to 100%) of specific methylation in this region are found in up to 30% of radiation- or carcinogen-induced thymic lymphomas, correlating with decreased gene expression. Hypermethylation of the p15INK4b 3′-UTR frequently occurs in tumors with loss of heterozygosity (LOH) but without methylation of the promoter CpG island or intragenic mutations. Furthermore, in vitro CpG methylation of the 3′-UTR produces reduced levels of a luciferase reporter in cultured cells. Methylation of two CpG sites in a 120 bp region is sufficient to interfere with transcription of the reporter gene. These data suggest that although the levels of p15INK4b in normal tissues can be mainly determined by promoter regulatory elements, strong hypermethylation of the 3′-UTR can interfere with transcription. Thus, hypermethylation of the 3′-UTR may explain the lack of p15INK4b gene expression in a subset of tumors with no promoter methylation and could be a new alternative mechanism for tumor suppressor gene inactivation in tumorigenesis. More... »

PAGES

385-396

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.onc.1202299

DOI

http://dx.doi.org/10.1038/sj.onc.1202299

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037254525

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9927195


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "3' Untranslated Regions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carrier Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclin-Dependent Kinase Inhibitor p15", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclin-Dependent Kinase Inhibitor p16", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclin-Dependent Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Methylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Neoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genomic Imprinting", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred Strains", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thymus Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Suppressor Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA", 
          "id": "http://www.grid.ac/institutes/grid.240324.3", 
          "name": [
            "Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Malumbres", 
        "givenName": "Marcos", 
        "id": "sg:person.0716321250.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716321250.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratorio de Gen\u00e9tica Molecular Humana, Dep. de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid, 28049, Cantoblanco, Madrid, Spain", 
          "id": "http://www.grid.ac/institutes/grid.5515.4", 
          "name": [
            "Laboratorio de Gen\u00e9tica Molecular Humana, Dep. de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid, 28049, Cantoblanco, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "P\u00e9rez de Castro", 
        "givenName": "Ignacio", 
        "id": "sg:person.01076345642.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076345642.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratorio de Gen\u00e9tica Molecular Humana, Dep. de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid, 28049, Cantoblanco, Madrid, Spain", 
          "id": "http://www.grid.ac/institutes/grid.5515.4", 
          "name": [
            "Laboratorio de Gen\u00e9tica Molecular Humana, Dep. de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid, 28049, Cantoblanco, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Santos", 
        "givenName": "Javier", 
        "id": "sg:person.011431467365.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011431467365.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratorio de Gen\u00e9tica Molecular Humana, Dep. de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid, 28049, Cantoblanco, Madrid, Spain", 
          "id": "http://www.grid.ac/institutes/grid.5515.4", 
          "name": [
            "Laboratorio de Gen\u00e9tica Molecular Humana, Dep. de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid, 28049, Cantoblanco, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fern\u00e1ndez Piqueras", 
        "givenName": "Jos\u00e9", 
        "id": "sg:person.01106347624.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106347624.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA", 
          "id": "http://www.grid.ac/institutes/grid.240324.3", 
          "name": [
            "Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pellicer", 
        "givenName": "Angel", 
        "id": "sg:person.012076377472.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012076377472.07"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/371204a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035500501", 
          "https://doi.org/10.1038/371204a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1095-204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005972301", 
          "https://doi.org/10.1038/ng1095-204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1200969", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020004260", 
          "https://doi.org/10.1038/sj.onc.1200969"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0795-686", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016345098", 
          "https://doi.org/10.1038/nm0795-686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/387417a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011555947", 
          "https://doi.org/10.1038/387417a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s003359900722", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048655666", 
          "https://doi.org/10.1007/s003359900722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/371257a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045553304", 
          "https://doi.org/10.1038/371257a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1095-210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014529417", 
          "https://doi.org/10.1038/ng1095-210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201178", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044089695", 
          "https://doi.org/10.1038/sj.onc.1201178"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-01-20", 
    "datePublishedReg": "1999-01-20", 
    "description": "The cyclin-dependent kinase inhibitor p15INK4b has been shown to be involved in human and rodent tumors and seems to act as a tumor suppressor gene in hematological malignancies. Alterations of this gene in tumors include mainly homozygous deletions and hypermethylation of the CpG island in the promoter region. In this work, we describe a new area sensitive to methylation in the 3\u2032 untranslated region (UTR) of the murine p15INK4b gene. This region shows different levels of methylation depending on the tissues, being relatively highly methylated in brain and gut, and weakly methylated in liver, spleen or thymus. DNA methylation and expression is similar in both maternal and paternal alleles indicating no imprinting effect. Although methylation of the p15INK4b 3\u2032-UTR is low in normal thymus, increased levels (up to 100%) of specific methylation in this region are found in up to 30% of radiation- or carcinogen-induced thymic lymphomas, correlating with decreased gene expression. Hypermethylation of the p15INK4b 3\u2032-UTR frequently occurs in tumors with loss of heterozygosity (LOH) but without methylation of the promoter CpG island or intragenic mutations. Furthermore, in vitro CpG methylation of the 3\u2032-UTR produces reduced levels of a luciferase reporter in cultured cells. Methylation of two CpG sites in a 120\u2009bp region is sufficient to interfere with transcription of the reporter gene. These data suggest that although the levels of p15INK4b in normal tissues can be mainly determined by promoter regulatory elements, strong hypermethylation of the 3\u2032-UTR can interfere with transcription. Thus, hypermethylation of the 3\u2032-UTR may explain the lack of p15INK4b gene expression in a subset of tumors with no promoter methylation and could be a new alternative mechanism for tumor suppressor gene inactivation in tumorigenesis.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/sj.onc.1202299", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2634269", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2467699", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2470266", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1097543", 
        "issn": [
          "0950-9232", 
          "1476-5594"
        ], 
        "name": "Oncogene", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "keywords": [
      "loss of heterozygosity", 
      "cell cycle inhibitor p15INK4b", 
      "subset of tumors", 
      "primary lymphoma", 
      "decreased gene expression", 
      "normal thymus", 
      "hematological malignancies", 
      "cyclin-dependent kinase inhibitor p15INK4B", 
      "rodent tumors", 
      "p15INK4b gene expression", 
      "thymic lymphomas", 
      "tumors", 
      "normal tissues", 
      "tumor suppressor gene", 
      "tumor suppressor gene inactivation", 
      "gene expression", 
      "luciferase reporter", 
      "suppressor gene inactivation", 
      "promoter methylation", 
      "reduced levels", 
      "lymphoma", 
      "homozygous deletion", 
      "thymus", 
      "suppressor gene", 
      "hypermethylation", 
      "p15INK4b", 
      "promoter CpG islands", 
      "new alternative mechanism", 
      "p15INK4B gene", 
      "cultured cells", 
      "intragenic mutations", 
      "strong hypermethylation", 
      "tissue", 
      "CpG islands", 
      "expression", 
      "CpG sites", 
      "untranslated region", 
      "levels", 
      "malignancy", 
      "spleen", 
      "promoter regulatory elements", 
      "promoter region", 
      "liver", 
      "genes", 
      "gene inactivation", 
      "brain", 
      "DNA methylation", 
      "reporter gene", 
      "paternal allele", 
      "CpG methylation", 
      "specific methylation", 
      "tumorigenesis", 
      "gut", 
      "methylation", 
      "alternative mechanism", 
      "alterations", 
      "transcriptional regulation", 
      "cells", 
      "humans", 
      "transcription", 
      "mutations", 
      "subset", 
      "alleles", 
      "reporter", 
      "inactivation", 
      "deletion", 
      "bp region", 
      "regulation", 
      "different levels", 
      "lack", 
      "loss", 
      "effect", 
      "regulatory elements", 
      "region", 
      "mechanism", 
      "heterozygosity", 
      "data", 
      "sites", 
      "new areas", 
      "imprinting effect", 
      "area", 
      "radiation", 
      "work", 
      "islands", 
      "elements", 
      "kinase inhibitor p15INK4b", 
      "inhibitor p15INK4b", 
      "murine p15INK4b gene", 
      "p15INK4b 3\u2032-UTR", 
      "carcinogen-induced thymic lymphomas", 
      "levels of p15INK4b", 
      "cycle inhibitor p15INK4b"
    ], 
    "name": "Hypermethylation of the cell cycle inhibitor p15INK4b 3\u2032-untranslated region interferes with its transcriptional regulation in primary lymphomas", 
    "pagination": "385-396", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037254525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.onc.1202299"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9927195"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.onc.1202299", 
      "https://app.dimensions.ai/details/publication/pub.1037254525"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_323.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/sj.onc.1202299"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202299'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202299'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202299'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1202299'


 

This table displays all metadata directly associated to this object as RDF triples.

307 TRIPLES      22 PREDICATES      147 URIs      129 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.onc.1202299 schema:about N009df89bff874cffb95b476403472dfe
2 N08043e3b305e48e1abe9b17b57634b95
3 N2ecc6e7b822d43eeb94703e16e3f5ab9
4 N33abfdacd2cc4b579d59901f0e945f2e
5 N3c3776ba2f7c4743ab91fdf6c8cc2b57
6 N3ca80d4574e243da8e65ed44fd3e4286
7 N432b1c5db2c64f08951835803c61fa71
8 N4fc1a665f6e341e69e4374d547cc691e
9 N549224e9b64c437dafd46e7c43981e45
10 N795e6436d105489f8a4a7f9191ae0f09
11 N9275b74073584b6797d092f7eb4e7720
12 Na455ff8c955e4ec1b661c46776542f86
13 Na981812498444d0c8108873781795922
14 Nafcbbf8a7d1045999d865e4eadb4958e
15 Nb03219de727e441ea3dd1d9c52bec23d
16 Nb25f614cd3d04321911789c38f201d13
17 Nbce5a202cd9f46e8a5a9b881ea8988d1
18 Ncb106658192947ee93b24e6223c34ea7
19 Nd52b68375fba4577a9c12d82a554c4fc
20 anzsrc-for:11
21 anzsrc-for:1103
22 anzsrc-for:1112
23 schema:author Nf04028b486d34918af7f63db77546582
24 schema:citation sg:pub.10.1007/s003359900722
25 sg:pub.10.1038/371204a0
26 sg:pub.10.1038/371257a0
27 sg:pub.10.1038/387417a0
28 sg:pub.10.1038/ng1095-204
29 sg:pub.10.1038/ng1095-210
30 sg:pub.10.1038/nm0795-686
31 sg:pub.10.1038/sj.onc.1200969
32 sg:pub.10.1038/sj.onc.1201178
33 schema:datePublished 1999-01-20
34 schema:datePublishedReg 1999-01-20
35 schema:description The cyclin-dependent kinase inhibitor p15INK4b has been shown to be involved in human and rodent tumors and seems to act as a tumor suppressor gene in hematological malignancies. Alterations of this gene in tumors include mainly homozygous deletions and hypermethylation of the CpG island in the promoter region. In this work, we describe a new area sensitive to methylation in the 3′ untranslated region (UTR) of the murine p15INK4b gene. This region shows different levels of methylation depending on the tissues, being relatively highly methylated in brain and gut, and weakly methylated in liver, spleen or thymus. DNA methylation and expression is similar in both maternal and paternal alleles indicating no imprinting effect. Although methylation of the p15INK4b 3′-UTR is low in normal thymus, increased levels (up to 100%) of specific methylation in this region are found in up to 30% of radiation- or carcinogen-induced thymic lymphomas, correlating with decreased gene expression. Hypermethylation of the p15INK4b 3′-UTR frequently occurs in tumors with loss of heterozygosity (LOH) but without methylation of the promoter CpG island or intragenic mutations. Furthermore, in vitro CpG methylation of the 3′-UTR produces reduced levels of a luciferase reporter in cultured cells. Methylation of two CpG sites in a 120 bp region is sufficient to interfere with transcription of the reporter gene. These data suggest that although the levels of p15INK4b in normal tissues can be mainly determined by promoter regulatory elements, strong hypermethylation of the 3′-UTR can interfere with transcription. Thus, hypermethylation of the 3′-UTR may explain the lack of p15INK4b gene expression in a subset of tumors with no promoter methylation and could be a new alternative mechanism for tumor suppressor gene inactivation in tumorigenesis.
36 schema:genre article
37 schema:inLanguage en
38 schema:isAccessibleForFree true
39 schema:isPartOf N2b76265eef7d4bfca9a44f75bac30d0f
40 N400ede25375c4be1af6da1c6bfabe6ab
41 sg:journal.1097543
42 schema:keywords CpG islands
43 CpG methylation
44 CpG sites
45 DNA methylation
46 alleles
47 alterations
48 alternative mechanism
49 area
50 bp region
51 brain
52 carcinogen-induced thymic lymphomas
53 cell cycle inhibitor p15INK4b
54 cells
55 cultured cells
56 cycle inhibitor p15INK4b
57 cyclin-dependent kinase inhibitor p15INK4B
58 data
59 decreased gene expression
60 deletion
61 different levels
62 effect
63 elements
64 expression
65 gene expression
66 gene inactivation
67 genes
68 gut
69 hematological malignancies
70 heterozygosity
71 homozygous deletion
72 humans
73 hypermethylation
74 imprinting effect
75 inactivation
76 inhibitor p15INK4b
77 intragenic mutations
78 islands
79 kinase inhibitor p15INK4b
80 lack
81 levels
82 levels of p15INK4b
83 liver
84 loss
85 loss of heterozygosity
86 luciferase reporter
87 lymphoma
88 malignancy
89 mechanism
90 methylation
91 murine p15INK4b gene
92 mutations
93 new alternative mechanism
94 new areas
95 normal thymus
96 normal tissues
97 p15INK4B gene
98 p15INK4b
99 p15INK4b 3′-UTR
100 p15INK4b gene expression
101 paternal allele
102 primary lymphoma
103 promoter CpG islands
104 promoter methylation
105 promoter region
106 promoter regulatory elements
107 radiation
108 reduced levels
109 region
110 regulation
111 regulatory elements
112 reporter
113 reporter gene
114 rodent tumors
115 sites
116 specific methylation
117 spleen
118 strong hypermethylation
119 subset
120 subset of tumors
121 suppressor gene
122 suppressor gene inactivation
123 thymic lymphomas
124 thymus
125 tissue
126 transcription
127 transcriptional regulation
128 tumor suppressor gene
129 tumor suppressor gene inactivation
130 tumorigenesis
131 tumors
132 untranslated region
133 work
134 schema:name Hypermethylation of the cell cycle inhibitor p15INK4b 3′-untranslated region interferes with its transcriptional regulation in primary lymphomas
135 schema:pagination 385-396
136 schema:productId N39034eeaf3254ded8ed402d70dfc8731
137 N6526168066be43439f53c7033dee9662
138 Nd81090a4ed5a40dba25acfe4b75536f4
139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037254525
140 https://doi.org/10.1038/sj.onc.1202299
141 schema:sdDatePublished 2021-11-01T18:03
142 schema:sdLicense https://scigraph.springernature.com/explorer/license/
143 schema:sdPublisher N61f919f8b0484831b1ce44bf0b95af72
144 schema:url https://doi.org/10.1038/sj.onc.1202299
145 sgo:license sg:explorer/license/
146 sgo:sdDataset articles
147 rdf:type schema:ScholarlyArticle
148 N009df89bff874cffb95b476403472dfe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Genomic Imprinting
150 rdf:type schema:DefinedTerm
151 N08043e3b305e48e1abe9b17b57634b95 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Cell Cycle Proteins
153 rdf:type schema:DefinedTerm
154 N2b76265eef7d4bfca9a44f75bac30d0f schema:issueNumber 2
155 rdf:type schema:PublicationIssue
156 N2ecc6e7b822d43eeb94703e16e3f5ab9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name DNA Methylation
158 rdf:type schema:DefinedTerm
159 N33abfdacd2cc4b579d59901f0e945f2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Gene Expression Regulation, Neoplastic
161 rdf:type schema:DefinedTerm
162 N34a5aec7306c4c72b2baa2e3429fd16e rdf:first sg:person.01106347624.32
163 rdf:rest N5194a277b04340209fc1bbfa9b8667bd
164 N373a67d531e54743863f33220babb7d0 rdf:first sg:person.01076345642.62
165 rdf:rest Nced74625d0624f8ab328d51abfdae420
166 N39034eeaf3254ded8ed402d70dfc8731 schema:name pubmed_id
167 schema:value 9927195
168 rdf:type schema:PropertyValue
169 N3c3776ba2f7c4743ab91fdf6c8cc2b57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Transcription, Genetic
171 rdf:type schema:DefinedTerm
172 N3ca80d4574e243da8e65ed44fd3e4286 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Base Sequence
174 rdf:type schema:DefinedTerm
175 N400ede25375c4be1af6da1c6bfabe6ab schema:volumeNumber 18
176 rdf:type schema:PublicationVolume
177 N432b1c5db2c64f08951835803c61fa71 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Cyclin-Dependent Kinase Inhibitor p15
179 rdf:type schema:DefinedTerm
180 N4fc1a665f6e341e69e4374d547cc691e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Animals
182 rdf:type schema:DefinedTerm
183 N5194a277b04340209fc1bbfa9b8667bd rdf:first sg:person.012076377472.07
184 rdf:rest rdf:nil
185 N549224e9b64c437dafd46e7c43981e45 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Thymus Neoplasms
187 rdf:type schema:DefinedTerm
188 N61f919f8b0484831b1ce44bf0b95af72 schema:name Springer Nature - SN SciGraph project
189 rdf:type schema:Organization
190 N6526168066be43439f53c7033dee9662 schema:name doi
191 schema:value 10.1038/sj.onc.1202299
192 rdf:type schema:PropertyValue
193 N795e6436d105489f8a4a7f9191ae0f09 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Molecular Sequence Data
195 rdf:type schema:DefinedTerm
196 N9275b74073584b6797d092f7eb4e7720 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
197 schema:name DNA Primers
198 rdf:type schema:DefinedTerm
199 Na455ff8c955e4ec1b661c46776542f86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Lymphoma
201 rdf:type schema:DefinedTerm
202 Na981812498444d0c8108873781795922 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
203 schema:name Tumor Suppressor Proteins
204 rdf:type schema:DefinedTerm
205 Nafcbbf8a7d1045999d865e4eadb4958e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
206 schema:name Mice, Inbred Strains
207 rdf:type schema:DefinedTerm
208 Nb03219de727e441ea3dd1d9c52bec23d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
209 schema:name 3' Untranslated Regions
210 rdf:type schema:DefinedTerm
211 Nb25f614cd3d04321911789c38f201d13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Carrier Proteins
213 rdf:type schema:DefinedTerm
214 Nbce5a202cd9f46e8a5a9b881ea8988d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
215 schema:name Cyclin-Dependent Kinases
216 rdf:type schema:DefinedTerm
217 Ncb106658192947ee93b24e6223c34ea7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
218 schema:name Mice
219 rdf:type schema:DefinedTerm
220 Nced74625d0624f8ab328d51abfdae420 rdf:first sg:person.011431467365.18
221 rdf:rest N34a5aec7306c4c72b2baa2e3429fd16e
222 Nd52b68375fba4577a9c12d82a554c4fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
223 schema:name Cyclin-Dependent Kinase Inhibitor p16
224 rdf:type schema:DefinedTerm
225 Nd81090a4ed5a40dba25acfe4b75536f4 schema:name dimensions_id
226 schema:value pub.1037254525
227 rdf:type schema:PropertyValue
228 Nf04028b486d34918af7f63db77546582 rdf:first sg:person.0716321250.19
229 rdf:rest N373a67d531e54743863f33220babb7d0
230 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
231 schema:name Medical and Health Sciences
232 rdf:type schema:DefinedTerm
233 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
234 schema:name Clinical Sciences
235 rdf:type schema:DefinedTerm
236 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
237 schema:name Oncology and Carcinogenesis
238 rdf:type schema:DefinedTerm
239 sg:grant.2467699 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.onc.1202299
240 rdf:type schema:MonetaryGrant
241 sg:grant.2470266 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.onc.1202299
242 rdf:type schema:MonetaryGrant
243 sg:grant.2634269 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.onc.1202299
244 rdf:type schema:MonetaryGrant
245 sg:journal.1097543 schema:issn 0950-9232
246 1476-5594
247 schema:name Oncogene
248 schema:publisher Springer Nature
249 rdf:type schema:Periodical
250 sg:person.01076345642.62 schema:affiliation grid-institutes:grid.5515.4
251 schema:familyName Pérez de Castro
252 schema:givenName Ignacio
253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076345642.62
254 rdf:type schema:Person
255 sg:person.01106347624.32 schema:affiliation grid-institutes:grid.5515.4
256 schema:familyName Fernández Piqueras
257 schema:givenName José
258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106347624.32
259 rdf:type schema:Person
260 sg:person.011431467365.18 schema:affiliation grid-institutes:grid.5515.4
261 schema:familyName Santos
262 schema:givenName Javier
263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011431467365.18
264 rdf:type schema:Person
265 sg:person.012076377472.07 schema:affiliation grid-institutes:grid.240324.3
266 schema:familyName Pellicer
267 schema:givenName Angel
268 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012076377472.07
269 rdf:type schema:Person
270 sg:person.0716321250.19 schema:affiliation grid-institutes:grid.240324.3
271 schema:familyName Malumbres
272 schema:givenName Marcos
273 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716321250.19
274 rdf:type schema:Person
275 sg:pub.10.1007/s003359900722 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048655666
276 https://doi.org/10.1007/s003359900722
277 rdf:type schema:CreativeWork
278 sg:pub.10.1038/371204a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035500501
279 https://doi.org/10.1038/371204a0
280 rdf:type schema:CreativeWork
281 sg:pub.10.1038/371257a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045553304
282 https://doi.org/10.1038/371257a0
283 rdf:type schema:CreativeWork
284 sg:pub.10.1038/387417a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011555947
285 https://doi.org/10.1038/387417a0
286 rdf:type schema:CreativeWork
287 sg:pub.10.1038/ng1095-204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005972301
288 https://doi.org/10.1038/ng1095-204
289 rdf:type schema:CreativeWork
290 sg:pub.10.1038/ng1095-210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014529417
291 https://doi.org/10.1038/ng1095-210
292 rdf:type schema:CreativeWork
293 sg:pub.10.1038/nm0795-686 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016345098
294 https://doi.org/10.1038/nm0795-686
295 rdf:type schema:CreativeWork
296 sg:pub.10.1038/sj.onc.1200969 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020004260
297 https://doi.org/10.1038/sj.onc.1200969
298 rdf:type schema:CreativeWork
299 sg:pub.10.1038/sj.onc.1201178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044089695
300 https://doi.org/10.1038/sj.onc.1201178
301 rdf:type schema:CreativeWork
302 grid-institutes:grid.240324.3 schema:alternateName Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA
303 schema:name Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA
304 rdf:type schema:Organization
305 grid-institutes:grid.5515.4 schema:alternateName Laboratorio de Genética Molecular Humana, Dep. de Biología, Universidad Autónoma de Madrid, 28049, Cantoblanco, Madrid, Spain
306 schema:name Laboratorio de Genética Molecular Humana, Dep. de Biología, Universidad Autónoma de Madrid, 28049, Cantoblanco, Madrid, Spain
307 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...