Recombinant ATM protein complements the cellular A-T phenotype View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1997-07

AUTHORS

Yael Ziv, Anat Bar-Shira, Iris Pecker, Pamela Russell, Timothy J Jorgensen, Ilan Tsarfati, Yosef Shiloh

ABSTRACT

Ataxia-telangiectasia (A-T) is an autosomal recessive disorder characterized by neurodegeneration, immunodeficiency, cancer predisposition, genome instability and radiation sensitivity. The cellular phenotype of A-T points to defects in signal transduction pathways involved in activation of cell cycle checkpoints by free radical damage, and other pathways that mediate the transmission of specific mitogenic stimuli. The product of the responsible gene, ATM, belongs to a family of large proteins that contribute to maintaining genome stability and cell cycle progression in various organisms. A recombinant vector that stably expresses a full-length ATM protein is a valuable tool for its functional analysis. We constructed and cloned a recombinant, full-length open reading frame of ATM using a combination of vectors and hosts that overcame an inherent instability of this sequence. Recombinant ATM was stably expressed in insect cells using a baculovirus vector, albeit at a low level, and in human A-T cells using an episomal expression vector. An amino-terminal FLAG epitope added to the protein allowed highly specific detection of the recombinant molecule by immunoblotting, immunoprecipitation and immunostaining, and its isolation using immunoaffinity. Similar to endogenous ATM, the recombinant protein is located mainly in the nucleus, with low levels in the cytoplasm. Ectopic expression of ATM in A-T cells restored normal sensitivity to ionizing radiation and the radiomimetic drug neocarzinostatin, and a normal pattern of post-irradiation DNA synthesis, which represents an S-phase checkpoint. These observations indicate that the recombinant, epitope-tagged protein is functional. Introduction into this molecule of a known A-T missense mutation, Glu2904Gly, resulted in apparent instability of the protein and inability to complement the A-T phenotype. These findings indicate that the physiological defects characteristic of A-T cells result from the absence of the ATM protein, and that this deficiency can be corrected by ectopic expression of this protein. More... »

PAGES

159-167

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.onc.1201319

DOI

http://dx.doi.org/10.1038/sj.onc.1201319

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007440313

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9244351


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ataxia Telangiectasia Mutated Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Open Reading Frames", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Biosynthesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein-Serine-Threonine Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spodoptera", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Suppressor Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel", 
          "id": "http://www.grid.ac/institutes/grid.12136.37", 
          "name": [
            "Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ziv", 
        "givenName": "Yael", 
        "id": "sg:person.0715664532.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715664532.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel", 
          "id": "http://www.grid.ac/institutes/grid.12136.37", 
          "name": [
            "Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bar-Shira", 
        "givenName": "Anat", 
        "id": "sg:person.01317711126.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317711126.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "InSight Strategy and Marketing Ltd., P.O.B. 2128, Rehovet, 76121, Israel", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel", 
            "InSight Strategy and Marketing Ltd., P.O.B. 2128, Rehovet, 76121, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pecker", 
        "givenName": "Iris", 
        "id": "sg:person.01163421213.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163421213.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Radiation Medicine, Vincent T Lombardi Cancer Research Center, Georgetown University Medical Center, 3970 Reservoir Road NW, 20007-2197, Washington, DC, USA", 
          "id": "http://www.grid.ac/institutes/grid.411667.3", 
          "name": [
            "Department of Radiation Medicine, Vincent T Lombardi Cancer Research Center, Georgetown University Medical Center, 3970 Reservoir Road NW, 20007-2197, Washington, DC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Russell", 
        "givenName": "Pamela", 
        "id": "sg:person.01227160234.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227160234.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Radiation Medicine, Vincent T Lombardi Cancer Research Center, Georgetown University Medical Center, 3970 Reservoir Road NW, 20007-2197, Washington, DC, USA", 
          "id": "http://www.grid.ac/institutes/grid.411667.3", 
          "name": [
            "Department of Radiation Medicine, Vincent T Lombardi Cancer Research Center, Georgetown University Medical Center, 3970 Reservoir Road NW, 20007-2197, Washington, DC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jorgensen", 
        "givenName": "Timothy J", 
        "id": "sg:person.01303264017.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303264017.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel", 
          "id": "http://www.grid.ac/institutes/grid.12136.37", 
          "name": [
            "Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsarfati", 
        "givenName": "Ilan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel", 
          "id": "http://www.grid.ac/institutes/grid.12136.37", 
          "name": [
            "Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shiloh", 
        "givenName": "Yosef", 
        "id": "sg:person.0720520054.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720520054.73"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1997-07", 
    "datePublishedReg": "1997-07-01", 
    "description": "Ataxia-telangiectasia (A-T) is an autosomal recessive disorder characterized by neurodegeneration, immunodeficiency, cancer predisposition, genome instability and radiation sensitivity. The cellular phenotype of A-T points to defects in signal transduction pathways involved in activation of cell cycle checkpoints by free radical damage, and other pathways that mediate the transmission of specific mitogenic stimuli. The product of the responsible gene, ATM, belongs to a family of large proteins that contribute to maintaining genome stability and cell cycle progression in various organisms. A recombinant vector that stably expresses a full-length ATM protein is a valuable tool for its functional analysis. We constructed and cloned a recombinant, full-length open reading frame of ATM using a combination of vectors and hosts that overcame an inherent instability of this sequence. Recombinant ATM was stably expressed in insect cells using a baculovirus vector, albeit at a low level, and in human A-T cells using an episomal expression vector. An amino-terminal FLAG epitope added to the protein allowed highly specific detection of the recombinant molecule by immunoblotting, immunoprecipitation and immunostaining, and its isolation using immunoaffinity. Similar to endogenous ATM, the recombinant protein is located mainly in the nucleus, with low levels in the cytoplasm. Ectopic expression of ATM in A-T cells restored normal sensitivity to ionizing radiation and the radiomimetic drug neocarzinostatin, and a normal pattern of post-irradiation DNA synthesis, which represents an S-phase checkpoint. These observations indicate that the recombinant, epitope-tagged protein is functional. Introduction into this molecule of a known A-T missense mutation, Glu2904Gly, resulted in apparent instability of the protein and inability to complement the A-T phenotype. These findings indicate that the physiological defects characteristic of A-T cells result from the absence of the ATM protein, and that this deficiency can be corrected by ectopic expression of this protein.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/sj.onc.1201319", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2557575", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1097543", 
        "issn": [
          "0950-9232", 
          "1476-5594"
        ], 
        "name": "Oncogene", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "keywords": [
      "ataxia-telangiectasia", 
      "ATM protein", 
      "ectopic expression", 
      "Recombinant ATM protein", 
      "cell cycle checkpoints", 
      "radiomimetic drug neocarzinostatin", 
      "signal transduction pathways", 
      "full-length ATM protein", 
      "cell cycle progression", 
      "episomal expression vector", 
      "recombinant ATM", 
      "genome stability", 
      "post-irradiation DNA synthesis", 
      "genome instability", 
      "phase checkpoint", 
      "endogenous ATM", 
      "cycle checkpoints", 
      "insect cells", 
      "cellular phenotype", 
      "transduction pathways", 
      "baculovirus vector", 
      "recombinant proteins", 
      "cycle progression", 
      "expression vector", 
      "functional analysis", 
      "physiological defects", 
      "specific mitogenic stimuli", 
      "FLAG epitope", 
      "responsible gene", 
      "recombinant molecules", 
      "large proteins", 
      "cancer predisposition", 
      "protein", 
      "missense mutations", 
      "recombinant vector", 
      "mitogenic stimuli", 
      "phenotype", 
      "DNA synthesis", 
      "autosomal recessive disorder", 
      "checkpoint", 
      "pathway", 
      "specific detection", 
      "recessive disorder", 
      "cells", 
      "free radical damage", 
      "radiation sensitivity", 
      "low levels", 
      "expression", 
      "normal sensitivity", 
      "radical damage", 
      "immunoprecipitation", 
      "genes", 
      "organisms", 
      "combination of vector", 
      "valuable tool", 
      "apparent instability", 
      "atm", 
      "cytoplasm", 
      "vector", 
      "mutations", 
      "normal pattern", 
      "molecules", 
      "sequence", 
      "host", 
      "neurodegeneration", 
      "activation", 
      "defects", 
      "family", 
      "isolation", 
      "immunoaffinity", 
      "neocarzinostatin", 
      "nucleus", 
      "inherent instability", 
      "epitopes", 
      "levels", 
      "absence", 
      "progression", 
      "deficiency", 
      "patterns", 
      "frame", 
      "damage", 
      "predisposition", 
      "synthesis", 
      "inability", 
      "immunodeficiency", 
      "sensitivity", 
      "instability", 
      "products", 
      "stimuli", 
      "analysis", 
      "combination", 
      "observations", 
      "tool", 
      "findings", 
      "radiation", 
      "transmission", 
      "disorders", 
      "stability", 
      "introduction", 
      "detection", 
      "point", 
      "frame of ATM", 
      "amino-terminal FLAG epitope", 
      "drug neocarzinostatin", 
      "Glu2904Gly"
    ], 
    "name": "Recombinant ATM protein complements the cellular A-T phenotype", 
    "pagination": "159-167", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1007440313"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.onc.1201319"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9244351"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.onc.1201319", 
      "https://app.dimensions.ai/details/publication/pub.1007440313"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_293.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/sj.onc.1201319"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1201319'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1201319'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1201319'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.onc.1201319'


 

This table displays all metadata directly associated to this object as RDF triples.

291 TRIPLES      21 PREDICATES      150 URIs      141 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.onc.1201319 schema:about N0615366e89de4730b5c949a9d535a137
2 N1330445c0beb4880ab0659750da731de
3 N1fa726c976674f7bae3ac9f086607876
4 N3a47ff9a04ae4185bf33b1b172585d91
5 N3e8acf55b5d34d148d4bd9e167c6040e
6 N4909bd5ecee44bb59786c6c67ea13bdf
7 N5323e03ea98e489c9d00d14b11c9b87b
8 N72a0c72c22fb40e1ac5268679fa46925
9 N7ab83df5921d47ec9754f910ead73f1e
10 N879b40974b4d4d13925b7d6f2b8b1471
11 N94ee5d58118945b4922fa28e6ba86012
12 N97aa786d6d4644adabc4415f361b5894
13 Nb01dc515828c4372917c7f96229c185d
14 Nb69458ee8f6740a6814e9b75f9d3363d
15 Nbe2ae9614fb9420c919c384262b1cea0
16 Nd97dbbba810d4835a9ff40378970591b
17 Ne32d45385ecc475398b9a9c0a64dd060
18 anzsrc-for:11
19 anzsrc-for:1103
20 anzsrc-for:1112
21 schema:author Nc86146d965144429ab7b8434318747a4
22 schema:datePublished 1997-07
23 schema:datePublishedReg 1997-07-01
24 schema:description Ataxia-telangiectasia (A-T) is an autosomal recessive disorder characterized by neurodegeneration, immunodeficiency, cancer predisposition, genome instability and radiation sensitivity. The cellular phenotype of A-T points to defects in signal transduction pathways involved in activation of cell cycle checkpoints by free radical damage, and other pathways that mediate the transmission of specific mitogenic stimuli. The product of the responsible gene, ATM, belongs to a family of large proteins that contribute to maintaining genome stability and cell cycle progression in various organisms. A recombinant vector that stably expresses a full-length ATM protein is a valuable tool for its functional analysis. We constructed and cloned a recombinant, full-length open reading frame of ATM using a combination of vectors and hosts that overcame an inherent instability of this sequence. Recombinant ATM was stably expressed in insect cells using a baculovirus vector, albeit at a low level, and in human A-T cells using an episomal expression vector. An amino-terminal FLAG epitope added to the protein allowed highly specific detection of the recombinant molecule by immunoblotting, immunoprecipitation and immunostaining, and its isolation using immunoaffinity. Similar to endogenous ATM, the recombinant protein is located mainly in the nucleus, with low levels in the cytoplasm. Ectopic expression of ATM in A-T cells restored normal sensitivity to ionizing radiation and the radiomimetic drug neocarzinostatin, and a normal pattern of post-irradiation DNA synthesis, which represents an S-phase checkpoint. These observations indicate that the recombinant, epitope-tagged protein is functional. Introduction into this molecule of a known A-T missense mutation, Glu2904Gly, resulted in apparent instability of the protein and inability to complement the A-T phenotype. These findings indicate that the physiological defects characteristic of A-T cells result from the absence of the ATM protein, and that this deficiency can be corrected by ectopic expression of this protein.
25 schema:genre article
26 schema:inLanguage en
27 schema:isAccessibleForFree true
28 schema:isPartOf N6735fe3a785b435796570d9f8331b9fb
29 Ndb079691af6b4fc8947f18c7406013a7
30 sg:journal.1097543
31 schema:keywords ATM protein
32 DNA synthesis
33 FLAG epitope
34 Glu2904Gly
35 Recombinant ATM protein
36 absence
37 activation
38 amino-terminal FLAG epitope
39 analysis
40 apparent instability
41 ataxia-telangiectasia
42 atm
43 autosomal recessive disorder
44 baculovirus vector
45 cancer predisposition
46 cell cycle checkpoints
47 cell cycle progression
48 cells
49 cellular phenotype
50 checkpoint
51 combination
52 combination of vector
53 cycle checkpoints
54 cycle progression
55 cytoplasm
56 damage
57 defects
58 deficiency
59 detection
60 disorders
61 drug neocarzinostatin
62 ectopic expression
63 endogenous ATM
64 episomal expression vector
65 epitopes
66 expression
67 expression vector
68 family
69 findings
70 frame
71 frame of ATM
72 free radical damage
73 full-length ATM protein
74 functional analysis
75 genes
76 genome instability
77 genome stability
78 host
79 immunoaffinity
80 immunodeficiency
81 immunoprecipitation
82 inability
83 inherent instability
84 insect cells
85 instability
86 introduction
87 isolation
88 large proteins
89 levels
90 low levels
91 missense mutations
92 mitogenic stimuli
93 molecules
94 mutations
95 neocarzinostatin
96 neurodegeneration
97 normal pattern
98 normal sensitivity
99 nucleus
100 observations
101 organisms
102 pathway
103 patterns
104 phase checkpoint
105 phenotype
106 physiological defects
107 point
108 post-irradiation DNA synthesis
109 predisposition
110 products
111 progression
112 protein
113 radiation
114 radiation sensitivity
115 radical damage
116 radiomimetic drug neocarzinostatin
117 recessive disorder
118 recombinant ATM
119 recombinant molecules
120 recombinant proteins
121 recombinant vector
122 responsible gene
123 sensitivity
124 sequence
125 signal transduction pathways
126 specific detection
127 specific mitogenic stimuli
128 stability
129 stimuli
130 synthesis
131 tool
132 transduction pathways
133 transmission
134 valuable tool
135 vector
136 schema:name Recombinant ATM protein complements the cellular A-T phenotype
137 schema:pagination 159-167
138 schema:productId N5b1e108420a846a9891711fbf7ded7ea
139 N7cf462d0750b43f8973059518d0ee4dd
140 Na3999d764435428782a68a51466f6aaa
141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007440313
142 https://doi.org/10.1038/sj.onc.1201319
143 schema:sdDatePublished 2021-12-01T19:10
144 schema:sdLicense https://scigraph.springernature.com/explorer/license/
145 schema:sdPublisher Nefe5e4dcde5d47d0b2fa4f3ccfdbe295
146 schema:url https://doi.org/10.1038/sj.onc.1201319
147 sgo:license sg:explorer/license/
148 sgo:sdDataset articles
149 rdf:type schema:ScholarlyArticle
150 N0243ee81eaec4752bc241b0f6934f905 rdf:first sg:person.0720520054.73
151 rdf:rest rdf:nil
152 N0615366e89de4730b5c949a9d535a137 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Humans
154 rdf:type schema:DefinedTerm
155 N1330445c0beb4880ab0659750da731de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Open Reading Frames
157 rdf:type schema:DefinedTerm
158 N1fa726c976674f7bae3ac9f086607876 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Mutation
160 rdf:type schema:DefinedTerm
161 N2405ce1fc87f45859888e5db2a5c9e25 rdf:first sg:person.01163421213.71
162 rdf:rest N6970d46f841f4ee697ffcb3530737efb
163 N3a47ff9a04ae4185bf33b1b172585d91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Proteins
165 rdf:type schema:DefinedTerm
166 N3e8acf55b5d34d148d4bd9e167c6040e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Phenotype
168 rdf:type schema:DefinedTerm
169 N48e06f9c683f4dab95730a73bff745d2 rdf:first Ne98311f8b5e844e5b30945e394672b8d
170 rdf:rest N0243ee81eaec4752bc241b0f6934f905
171 N4909bd5ecee44bb59786c6c67ea13bdf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Ataxia Telangiectasia Mutated Proteins
173 rdf:type schema:DefinedTerm
174 N5323e03ea98e489c9d00d14b11c9b87b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Rabbits
176 rdf:type schema:DefinedTerm
177 N5b1e108420a846a9891711fbf7ded7ea schema:name doi
178 schema:value 10.1038/sj.onc.1201319
179 rdf:type schema:PropertyValue
180 N6735fe3a785b435796570d9f8331b9fb schema:issueNumber 2
181 rdf:type schema:PublicationIssue
182 N6970d46f841f4ee697ffcb3530737efb rdf:first sg:person.01227160234.41
183 rdf:rest N6c34cfed24ba4b81b9dd352ea79d91d1
184 N6c34cfed24ba4b81b9dd352ea79d91d1 rdf:first sg:person.01303264017.42
185 rdf:rest N48e06f9c683f4dab95730a73bff745d2
186 N72a0c72c22fb40e1ac5268679fa46925 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Cell Cycle Proteins
188 rdf:type schema:DefinedTerm
189 N7ab83df5921d47ec9754f910ead73f1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
190 schema:name Animals
191 rdf:type schema:DefinedTerm
192 N7c897d6638f6450ab2526e9fcd9e7c19 rdf:first sg:person.01317711126.96
193 rdf:rest N2405ce1fc87f45859888e5db2a5c9e25
194 N7cf462d0750b43f8973059518d0ee4dd schema:name pubmed_id
195 schema:value 9244351
196 rdf:type schema:PropertyValue
197 N879b40974b4d4d13925b7d6f2b8b1471 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Tumor Suppressor Proteins
199 rdf:type schema:DefinedTerm
200 N94ee5d58118945b4922fa28e6ba86012 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
201 schema:name Cloning, Molecular
202 rdf:type schema:DefinedTerm
203 N97aa786d6d4644adabc4415f361b5894 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
204 schema:name Spodoptera
205 rdf:type schema:DefinedTerm
206 Na3999d764435428782a68a51466f6aaa schema:name dimensions_id
207 schema:value pub.1007440313
208 rdf:type schema:PropertyValue
209 Nb01dc515828c4372917c7f96229c185d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name Recombinant Proteins
211 rdf:type schema:DefinedTerm
212 Nb69458ee8f6740a6814e9b75f9d3363d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
213 schema:name Cell Line
214 rdf:type schema:DefinedTerm
215 Nbe2ae9614fb9420c919c384262b1cea0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
216 schema:name Protein-Serine-Threonine Kinases
217 rdf:type schema:DefinedTerm
218 Nc86146d965144429ab7b8434318747a4 rdf:first sg:person.0715664532.97
219 rdf:rest N7c897d6638f6450ab2526e9fcd9e7c19
220 Nd97dbbba810d4835a9ff40378970591b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
221 schema:name DNA-Binding Proteins
222 rdf:type schema:DefinedTerm
223 Ndb079691af6b4fc8947f18c7406013a7 schema:volumeNumber 15
224 rdf:type schema:PublicationVolume
225 Ne32d45385ecc475398b9a9c0a64dd060 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
226 schema:name Protein Biosynthesis
227 rdf:type schema:DefinedTerm
228 Ne98311f8b5e844e5b30945e394672b8d schema:affiliation grid-institutes:grid.12136.37
229 schema:familyName Tsarfati
230 schema:givenName Ilan
231 rdf:type schema:Person
232 Nefe5e4dcde5d47d0b2fa4f3ccfdbe295 schema:name Springer Nature - SN SciGraph project
233 rdf:type schema:Organization
234 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
235 schema:name Medical and Health Sciences
236 rdf:type schema:DefinedTerm
237 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
238 schema:name Clinical Sciences
239 rdf:type schema:DefinedTerm
240 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
241 schema:name Oncology and Carcinogenesis
242 rdf:type schema:DefinedTerm
243 sg:grant.2557575 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.onc.1201319
244 rdf:type schema:MonetaryGrant
245 sg:journal.1097543 schema:issn 0950-9232
246 1476-5594
247 schema:name Oncogene
248 schema:publisher Springer Nature
249 rdf:type schema:Periodical
250 sg:person.01163421213.71 schema:affiliation grid-institutes:None
251 schema:familyName Pecker
252 schema:givenName Iris
253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163421213.71
254 rdf:type schema:Person
255 sg:person.01227160234.41 schema:affiliation grid-institutes:grid.411667.3
256 schema:familyName Russell
257 schema:givenName Pamela
258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227160234.41
259 rdf:type schema:Person
260 sg:person.01303264017.42 schema:affiliation grid-institutes:grid.411667.3
261 schema:familyName Jorgensen
262 schema:givenName Timothy J
263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303264017.42
264 rdf:type schema:Person
265 sg:person.01317711126.96 schema:affiliation grid-institutes:grid.12136.37
266 schema:familyName Bar-Shira
267 schema:givenName Anat
268 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317711126.96
269 rdf:type schema:Person
270 sg:person.0715664532.97 schema:affiliation grid-institutes:grid.12136.37
271 schema:familyName Ziv
272 schema:givenName Yael
273 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715664532.97
274 rdf:type schema:Person
275 sg:person.0720520054.73 schema:affiliation grid-institutes:grid.12136.37
276 schema:familyName Shiloh
277 schema:givenName Yosef
278 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720520054.73
279 rdf:type schema:Person
280 grid-institutes:None schema:alternateName InSight Strategy and Marketing Ltd., P.O.B. 2128, Rehovet, 76121, Israel
281 schema:name Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel
282 InSight Strategy and Marketing Ltd., P.O.B. 2128, Rehovet, 76121, Israel
283 rdf:type schema:Organization
284 grid-institutes:grid.12136.37 schema:alternateName Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel
285 Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel
286 schema:name Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel
287 Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel
288 rdf:type schema:Organization
289 grid-institutes:grid.411667.3 schema:alternateName Department of Radiation Medicine, Vincent T Lombardi Cancer Research Center, Georgetown University Medical Center, 3970 Reservoir Road NW, 20007-2197, Washington, DC, USA
290 schema:name Department of Radiation Medicine, Vincent T Lombardi Cancer Research Center, Georgetown University Medical Center, 3970 Reservoir Road NW, 20007-2197, Washington, DC, USA
291 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...