Incomplete DJH rearrangements of the IgH gene are frequent in multiple myeloma patients: immunobiological characteristics and clinical implications View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-07

AUTHORS

D González, A Balanzategui, R García-Sanz, N Gutiérrez, C Seabra, J J M van Dongen, M González, J F San Miguel

ABSTRACT

DH-JH rearrangements of the Ig heavy-chain gene (IGH) occur early during B-cell development. Consequently, they are detected in precursor-B-cell acute lymphoblastic leukemias both at diagnosis and relapse. Incomplete DJH rearrangements have also been occasionally reported in mature B-cell lymphoproliferative disorders, but their frequency and immunobiological characteristics have not been studied in detail. We have investigated the frequency and characteristics of incomplete DJH as well as complete VDJH rearrangements in a series of 84 untreated multiple myeloma (MM) patients. The overall detection rate of clonality by amplifying VDJH and DJH rearrangements using family-specific primers was 94%. Interestingly, we found a high frequency (60%) of DJH rearrangements in this group. As expected from an immunological point of view, the vast majority of DJH rearrangements (88%) were unmutated. To the best of our knowledge, this is the first systematic study describing the incidence of incomplete DJH rearrangements in a series of unselected MM patients. These results strongly support the use of DJH rearrangements as PCR targets for clonality studies and, particularly, for quantification of minimal residual disease by real-time quantitative PCR using consensus JH probes in MM patients. The finding of hypermutation in a small proportion of incomplete DJH rearrangements (six out of 50) suggests important biological implications concerning the process of somatic hypermutation. Moreover, our data offer a new insight in the regulatory development model of IGH rearrangements. More... »

PAGES

2402964

Journal

TITLE

Leukemia

ISSUE

7

VOLUME

17

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.leu.2402964

    DOI

    http://dx.doi.org/10.1038/sj.leu.2402964

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034422034

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12835730


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