Stromal cells from murine embryonic aorta–gonad–mesonephros region, liver and gut mesentery expand human umbilical cord blood-derived CAFCweek6 in extended long-term ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2002-09

AUTHORS

N Kusadasi, RAJ Oostendorp, WJLM Koevoet, EA Dzierzak, RE Ploemacher

ABSTRACT

The first definitive long-term repopulating hematopoietic stem cells (HSCs) emerge from and undergo rapid expansion in the embryonic aorta-gonad-mesonephros (AGM) region. To investigate the presumptive unique characteristics of the embryonic hematopoietic microenvironment and its surrounding tissues, we have generated stromal clones from subdissected day 10 and day 11 AGMs, embryonic livers (ELs) and gut mesentery. We here examine the ability of 19 of these clones to sustain extended long-term cultures (LTCs) of human CD34(+) umbilical cord blood (UCB) cells in vitro. The presence of in vitro repopulating cells was assessed by sustained production of progenitor cells (extended LTC-CFC) and cobblestone area-forming cells (CAFC). The embryonic stromal clones differed greatly in their support for human HSCs. Out of eight clones tested in the absence of exogenous cytokines, only one (EL-derived) clone was able to provide maintenance of HSCs. Addition of either Tpo or Flt3-L + Tpo improved the long-term support of about 50% of the tested clones. Cultures on four out of 19 clones, ie the EL-derived clone mentioned, two urogenital-ridge (UG)-derived clones and one gastrointestinal (GI)-derived clone, allowed a continuous expansion of primitive CAFC and CFU-GM with over several hundred-fold more CAFC(week6) produced in the 12th week of culture. This expansion was considerably higher than that found with the FBMD-1 cell line, which is appreciated by many investigators for its support of human HSCs, under comparable conditions. This stromal cell panel derived from the embryonic regions may be a powerful tool in dissecting the factors mediating stromal support for maintenance and expansion of HSCs. More... »

PAGES

1782

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.leu.2402615

DOI

http://dx.doi.org/10.1038/sj.leu.2402615

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047951257

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12200694


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD34", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aorta", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Lineage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Separation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clone Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coculture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colony-Forming Units Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytokines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Digestive System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo, Mammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fetal Blood", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gonads", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Graft Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hematopoiesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesentery", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesonephros", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Transgenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stromal Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kusadasi", 
        "givenName": "N", 
        "id": "sg:person.01244223270.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244223270.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Cell Biology and Genetics, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oostendorp", 
        "givenName": "RAJ", 
        "id": "sg:person.013304220014.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013304220014.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koevoet", 
        "givenName": "WJLM", 
        "id": "sg:person.01226564337.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226564337.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Cell Biology and Genetics, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dzierzak", 
        "givenName": "EA", 
        "id": "sg:person.01012155451.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012155451.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ploemacher", 
        "givenName": "RE", 
        "id": "sg:person.014316077474.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014316077474.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00653-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000102997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003126954", 
          "https://doi.org/10.1038/sj.leu.2401432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003126954", 
          "https://doi.org/10.1038/sj.leu.2401432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.11.2465", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006446860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2402204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010238804", 
          "https://doi.org/10.1038/sj.leu.2402204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2402204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010238804", 
          "https://doi.org/10.1038/sj.leu.2402204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.186.4.619", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010498871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80267-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010531915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00557-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010859962"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1041300121", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011977373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/382635a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016281241", 
          "https://doi.org/10.1038/382635a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017219884", 
          "https://doi.org/10.1038/sj.leu.2401003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017219884", 
          "https://doi.org/10.1038/sj.leu.2401003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80165-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023382146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.22.10302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032163309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80167-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033920828"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040910303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036776047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.185.7.1163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039547742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.14.8126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040869632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041729054", 
          "https://doi.org/10.1038/sj.leu.2401917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041729054", 
          "https://doi.org/10.1038/sj.leu.2401917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2141.1985.tb07393.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041931249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00515-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042892402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood.v99.4.1183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043466370"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.77.5.2927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047299727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1986.tb00391.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050243033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bmt.1702634", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050609506", 
          "https://doi.org/10.1038/sj.bmt.1702634"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8342023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062654735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074463122", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074601297", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074610541", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075039946", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079409629", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080072738", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082620066", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082889332", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083073897", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083105702", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083118290", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083274021", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083296922", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083314035", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083354363", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-09", 
    "datePublishedReg": "2002-09-01", 
    "description": "The first definitive long-term repopulating hematopoietic stem cells (HSCs) emerge from and undergo rapid expansion in the embryonic aorta-gonad-mesonephros (AGM) region. To investigate the presumptive unique characteristics of the embryonic hematopoietic microenvironment and its surrounding tissues, we have generated stromal clones from subdissected day 10 and day 11 AGMs, embryonic livers (ELs) and gut mesentery. We here examine the ability of 19 of these clones to sustain extended long-term cultures (LTCs) of human CD34(+) umbilical cord blood (UCB) cells in vitro. The presence of in vitro repopulating cells was assessed by sustained production of progenitor cells (extended LTC-CFC) and cobblestone area-forming cells (CAFC). The embryonic stromal clones differed greatly in their support for human HSCs. Out of eight clones tested in the absence of exogenous cytokines, only one (EL-derived) clone was able to provide maintenance of HSCs. Addition of either Tpo or Flt3-L + Tpo improved the long-term support of about 50% of the tested clones. Cultures on four out of 19 clones, ie the EL-derived clone mentioned, two urogenital-ridge (UG)-derived clones and one gastrointestinal (GI)-derived clone, allowed a continuous expansion of primitive CAFC and CFU-GM with over several hundred-fold more CAFC(week6) produced in the 12th week of culture. This expansion was considerably higher than that found with the FBMD-1 cell line, which is appreciated by many investigators for its support of human HSCs, under comparable conditions. This stromal cell panel derived from the embryonic regions may be a powerful tool in dissecting the factors mediating stromal support for maintenance and expansion of HSCs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.leu.2402615", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1097065", 
        "issn": [
          "0887-6924", 
          "1476-5551"
        ], 
        "name": "Leukemia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "16"
      }
    ], 
    "name": "Stromal cells from murine embryonic aorta\u2013gonad\u2013mesonephros region, liver and gut mesentery expand human umbilical cord blood-derived CAFCweek6 in extended long-term cultures", 
    "pagination": "1782", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e46dc2221816be281f584db1028021b45e5f3efb444950ad749a60bad2ec3ecc"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12200694"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8704895"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.leu.2402615"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1047951257"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.leu.2402615", 
      "https://app.dimensions.ai/details/publication/pub.1047951257"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29222_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/2402615"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'


 

This table displays all metadata directly associated to this object as RDF triples.

302 TRIPLES      21 PREDICATES      92 URIs      45 LITERALS      33 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.leu.2402615 schema:about N0199cff235c04c14acb45bae972fafda
2 N24d647f5792545ea8b5ae325de3e73ff
3 N2660962d37104bcd9a9e5179339a523b
4 N3449906faa9548fca8e23789e897bbf2
5 N441f471dc03149c7af17a41697d615e0
6 N44aa87191ec840529d302ea7cdaf42a0
7 N6c9823547c674a7eac59d5e09da13fd4
8 N700262d75695400d9bd1790b0ccc06d4
9 N7282f3c169134839b49031b7ddf734fa
10 N76c6f731b15841c19cc480a6c21da6bb
11 N7840515754194e4ca3ade8d32551684b
12 N93c929e3e4164c168354930c40e15c04
13 N93f64290681a4dd8b006adc779cd0cbf
14 Nb6e00a5d85b54d5ea0c81258663be30e
15 Nb77d82592cb044b480a29756be693621
16 Nbb5ccd793962435ea5d71098614b9277
17 Nc2838acb851941c8b04ac6ab95ffd46b
18 Nccfe1f62b4c345eaa9c8d6f36d42ca0a
19 Ncd5abdbe363f464b9c006de16fcf5172
20 Nda3e88e992524d04a21e0386c6257841
21 Nde863931aef745c4a4d0369c2903e891
22 Nefd714294a28433ba4a757df532d95b8
23 Nfab4963c41d1410c976e5c28e1074b01
24 Nfd1bfedc708947e999975ccb93e203b4
25 anzsrc-for:06
26 anzsrc-for:0601
27 schema:author Nfe736834e23b43b0b1b2625eae8cf9d3
28 schema:citation sg:pub.10.1038/382635a0
29 sg:pub.10.1038/sj.bmt.1702634
30 sg:pub.10.1038/sj.leu.2401003
31 sg:pub.10.1038/sj.leu.2401432
32 sg:pub.10.1038/sj.leu.2401917
33 sg:pub.10.1038/sj.leu.2402204
34 https://app.dimensions.ai/details/publication/pub.1074463122
35 https://app.dimensions.ai/details/publication/pub.1074601297
36 https://app.dimensions.ai/details/publication/pub.1074610541
37 https://app.dimensions.ai/details/publication/pub.1075039946
38 https://app.dimensions.ai/details/publication/pub.1079409629
39 https://app.dimensions.ai/details/publication/pub.1080072738
40 https://app.dimensions.ai/details/publication/pub.1082620066
41 https://app.dimensions.ai/details/publication/pub.1082889332
42 https://app.dimensions.ai/details/publication/pub.1083073897
43 https://app.dimensions.ai/details/publication/pub.1083105702
44 https://app.dimensions.ai/details/publication/pub.1083118290
45 https://app.dimensions.ai/details/publication/pub.1083274021
46 https://app.dimensions.ai/details/publication/pub.1083296922
47 https://app.dimensions.ai/details/publication/pub.1083314035
48 https://app.dimensions.ai/details/publication/pub.1083354363
49 https://doi.org/10.1002/jcp.1040910303
50 https://doi.org/10.1002/jcp.1041300121
51 https://doi.org/10.1016/s0092-8674(00)80165-8
52 https://doi.org/10.1016/s0301-472x(00)00515-4
53 https://doi.org/10.1016/s0301-472x(00)00557-9
54 https://doi.org/10.1016/s0301-472x(00)00653-6
55 https://doi.org/10.1016/s1074-7613(00)80167-3
56 https://doi.org/10.1016/s1074-7613(00)80267-8
57 https://doi.org/10.1073/pnas.77.5.2927
58 https://doi.org/10.1073/pnas.92.22.10302
59 https://doi.org/10.1073/pnas.96.14.8126
60 https://doi.org/10.1084/jem.185.7.1163
61 https://doi.org/10.1084/jem.186.4.619
62 https://doi.org/10.1093/emboj/19.11.2465
63 https://doi.org/10.1111/j.1365-2141.1985.tb07393.x
64 https://doi.org/10.1111/j.1432-0436.1986.tb00391.x
65 https://doi.org/10.1126/science.8342023
66 https://doi.org/10.1182/blood.v99.4.1183
67 schema:datePublished 2002-09
68 schema:datePublishedReg 2002-09-01
69 schema:description The first definitive long-term repopulating hematopoietic stem cells (HSCs) emerge from and undergo rapid expansion in the embryonic aorta-gonad-mesonephros (AGM) region. To investigate the presumptive unique characteristics of the embryonic hematopoietic microenvironment and its surrounding tissues, we have generated stromal clones from subdissected day 10 and day 11 AGMs, embryonic livers (ELs) and gut mesentery. We here examine the ability of 19 of these clones to sustain extended long-term cultures (LTCs) of human CD34(+) umbilical cord blood (UCB) cells in vitro. The presence of in vitro repopulating cells was assessed by sustained production of progenitor cells (extended LTC-CFC) and cobblestone area-forming cells (CAFC). The embryonic stromal clones differed greatly in their support for human HSCs. Out of eight clones tested in the absence of exogenous cytokines, only one (EL-derived) clone was able to provide maintenance of HSCs. Addition of either Tpo or Flt3-L + Tpo improved the long-term support of about 50% of the tested clones. Cultures on four out of 19 clones, ie the EL-derived clone mentioned, two urogenital-ridge (UG)-derived clones and one gastrointestinal (GI)-derived clone, allowed a continuous expansion of primitive CAFC and CFU-GM with over several hundred-fold more CAFC(week6) produced in the 12th week of culture. This expansion was considerably higher than that found with the FBMD-1 cell line, which is appreciated by many investigators for its support of human HSCs, under comparable conditions. This stromal cell panel derived from the embryonic regions may be a powerful tool in dissecting the factors mediating stromal support for maintenance and expansion of HSCs.
70 schema:genre research_article
71 schema:inLanguage en
72 schema:isAccessibleForFree true
73 schema:isPartOf N24062ef9b5074065a91bf6a5d587d6cb
74 N76072e2d6946489296cfcdf6c7d86949
75 sg:journal.1097065
76 schema:name Stromal cells from murine embryonic aorta–gonad–mesonephros region, liver and gut mesentery expand human umbilical cord blood-derived CAFCweek6 in extended long-term cultures
77 schema:pagination 1782
78 schema:productId N455fda7c0bd74c59be96cc341300903b
79 N46ec8e9f8f3541b7bdb8679b01f3ab4e
80 N5a94b91f80ce4a55b1349fb0ff5f44df
81 Ncdf6be214c1447959c648d9fbab4a7bc
82 Ne93fcf141cee4de9bb46dd8041fb4325
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047951257
84 https://doi.org/10.1038/sj.leu.2402615
85 schema:sdDatePublished 2019-04-11T11:58
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher Na691553d331f45e0968a128cf6ead7d4
88 schema:url https://www.nature.com/articles/2402615
89 sgo:license sg:explorer/license/
90 sgo:sdDataset articles
91 rdf:type schema:ScholarlyArticle
92 N0199cff235c04c14acb45bae972fafda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Colony-Forming Units Assay
94 rdf:type schema:DefinedTerm
95 N04d64042f4ac41988564536761b19d68 rdf:first sg:person.01226564337.27
96 rdf:rest N2670ee57fcff403e9d824b49b0f03f66
97 N24062ef9b5074065a91bf6a5d587d6cb schema:issueNumber 9
98 rdf:type schema:PublicationIssue
99 N24d647f5792545ea8b5ae325de3e73ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Liver
101 rdf:type schema:DefinedTerm
102 N2660962d37104bcd9a9e5179339a523b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Reverse Transcriptase Polymerase Chain Reaction
104 rdf:type schema:DefinedTerm
105 N2670ee57fcff403e9d824b49b0f03f66 rdf:first sg:person.01012155451.06
106 rdf:rest N9cfb767f80f94d7e9779e2c859b9a0c0
107 N3449906faa9548fca8e23789e897bbf2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Stem Cells
109 rdf:type schema:DefinedTerm
110 N44048edc00a047d6a6ea05be56da5c10 rdf:first sg:person.013304220014.01
111 rdf:rest N04d64042f4ac41988564536761b19d68
112 N441f471dc03149c7af17a41697d615e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Graft Survival
114 rdf:type schema:DefinedTerm
115 N44aa87191ec840529d302ea7cdaf42a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Stromal Cells
117 rdf:type schema:DefinedTerm
118 N455fda7c0bd74c59be96cc341300903b schema:name nlm_unique_id
119 schema:value 8704895
120 rdf:type schema:PropertyValue
121 N46ec8e9f8f3541b7bdb8679b01f3ab4e schema:name readcube_id
122 schema:value e46dc2221816be281f584db1028021b45e5f3efb444950ad749a60bad2ec3ecc
123 rdf:type schema:PropertyValue
124 N5a94b91f80ce4a55b1349fb0ff5f44df schema:name doi
125 schema:value 10.1038/sj.leu.2402615
126 rdf:type schema:PropertyValue
127 N6c9823547c674a7eac59d5e09da13fd4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Fetal Blood
129 rdf:type schema:DefinedTerm
130 N700262d75695400d9bd1790b0ccc06d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Cytokines
132 rdf:type schema:DefinedTerm
133 N7282f3c169134839b49031b7ddf734fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Cell Separation
135 rdf:type schema:DefinedTerm
136 N76072e2d6946489296cfcdf6c7d86949 schema:volumeNumber 16
137 rdf:type schema:PublicationVolume
138 N76c6f731b15841c19cc480a6c21da6bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Mice
140 rdf:type schema:DefinedTerm
141 N7840515754194e4ca3ade8d32551684b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Gonads
143 rdf:type schema:DefinedTerm
144 N93c929e3e4164c168354930c40e15c04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Antigens, CD34
146 rdf:type schema:DefinedTerm
147 N93f64290681a4dd8b006adc779cd0cbf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Coculture Techniques
149 rdf:type schema:DefinedTerm
150 N9cfb767f80f94d7e9779e2c859b9a0c0 rdf:first sg:person.014316077474.52
151 rdf:rest rdf:nil
152 Na691553d331f45e0968a128cf6ead7d4 schema:name Springer Nature - SN SciGraph project
153 rdf:type schema:Organization
154 Nb6e00a5d85b54d5ea0c81258663be30e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Clone Cells
156 rdf:type schema:DefinedTerm
157 Nb77d82592cb044b480a29756be693621 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Animals
159 rdf:type schema:DefinedTerm
160 Nbb5ccd793962435ea5d71098614b9277 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Mesentery
162 rdf:type schema:DefinedTerm
163 Nc2838acb851941c8b04ac6ab95ffd46b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Humans
165 rdf:type schema:DefinedTerm
166 Nccfe1f62b4c345eaa9c8d6f36d42ca0a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Hematopoiesis
168 rdf:type schema:DefinedTerm
169 Ncd5abdbe363f464b9c006de16fcf5172 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Mesonephros
171 rdf:type schema:DefinedTerm
172 Ncdf6be214c1447959c648d9fbab4a7bc schema:name pubmed_id
173 schema:value 12200694
174 rdf:type schema:PropertyValue
175 Nda3e88e992524d04a21e0386c6257841 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Mice, Transgenic
177 rdf:type schema:DefinedTerm
178 Nde863931aef745c4a4d0369c2903e891 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Embryo, Mammalian
180 rdf:type schema:DefinedTerm
181 Ne93fcf141cee4de9bb46dd8041fb4325 schema:name dimensions_id
182 schema:value pub.1047951257
183 rdf:type schema:PropertyValue
184 Nefd714294a28433ba4a757df532d95b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Digestive System
186 rdf:type schema:DefinedTerm
187 Nfab4963c41d1410c976e5c28e1074b01 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name Aorta
189 rdf:type schema:DefinedTerm
190 Nfd1bfedc708947e999975ccb93e203b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Cell Lineage
192 rdf:type schema:DefinedTerm
193 Nfe736834e23b43b0b1b2625eae8cf9d3 rdf:first sg:person.01244223270.13
194 rdf:rest N44048edc00a047d6a6ea05be56da5c10
195 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
196 schema:name Biological Sciences
197 rdf:type schema:DefinedTerm
198 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
199 schema:name Biochemistry and Cell Biology
200 rdf:type schema:DefinedTerm
201 sg:journal.1097065 schema:issn 0887-6924
202 1476-5551
203 schema:name Leukemia
204 rdf:type schema:Periodical
205 sg:person.01012155451.06 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
206 schema:familyName Dzierzak
207 schema:givenName EA
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012155451.06
209 rdf:type schema:Person
210 sg:person.01226564337.27 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
211 schema:familyName Koevoet
212 schema:givenName WJLM
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226564337.27
214 rdf:type schema:Person
215 sg:person.01244223270.13 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
216 schema:familyName Kusadasi
217 schema:givenName N
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244223270.13
219 rdf:type schema:Person
220 sg:person.013304220014.01 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
221 schema:familyName Oostendorp
222 schema:givenName RAJ
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013304220014.01
224 rdf:type schema:Person
225 sg:person.014316077474.52 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
226 schema:familyName Ploemacher
227 schema:givenName RE
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014316077474.52
229 rdf:type schema:Person
230 sg:pub.10.1038/382635a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016281241
231 https://doi.org/10.1038/382635a0
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/sj.bmt.1702634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050609506
234 https://doi.org/10.1038/sj.bmt.1702634
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/sj.leu.2401003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017219884
237 https://doi.org/10.1038/sj.leu.2401003
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/sj.leu.2401432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003126954
240 https://doi.org/10.1038/sj.leu.2401432
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/sj.leu.2401917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041729054
243 https://doi.org/10.1038/sj.leu.2401917
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/sj.leu.2402204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010238804
246 https://doi.org/10.1038/sj.leu.2402204
247 rdf:type schema:CreativeWork
248 https://app.dimensions.ai/details/publication/pub.1074463122 schema:CreativeWork
249 https://app.dimensions.ai/details/publication/pub.1074601297 schema:CreativeWork
250 https://app.dimensions.ai/details/publication/pub.1074610541 schema:CreativeWork
251 https://app.dimensions.ai/details/publication/pub.1075039946 schema:CreativeWork
252 https://app.dimensions.ai/details/publication/pub.1079409629 schema:CreativeWork
253 https://app.dimensions.ai/details/publication/pub.1080072738 schema:CreativeWork
254 https://app.dimensions.ai/details/publication/pub.1082620066 schema:CreativeWork
255 https://app.dimensions.ai/details/publication/pub.1082889332 schema:CreativeWork
256 https://app.dimensions.ai/details/publication/pub.1083073897 schema:CreativeWork
257 https://app.dimensions.ai/details/publication/pub.1083105702 schema:CreativeWork
258 https://app.dimensions.ai/details/publication/pub.1083118290 schema:CreativeWork
259 https://app.dimensions.ai/details/publication/pub.1083274021 schema:CreativeWork
260 https://app.dimensions.ai/details/publication/pub.1083296922 schema:CreativeWork
261 https://app.dimensions.ai/details/publication/pub.1083314035 schema:CreativeWork
262 https://app.dimensions.ai/details/publication/pub.1083354363 schema:CreativeWork
263 https://doi.org/10.1002/jcp.1040910303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036776047
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1002/jcp.1041300121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011977373
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/s0092-8674(00)80165-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023382146
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/s0301-472x(00)00515-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042892402
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1016/s0301-472x(00)00557-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010859962
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1016/s0301-472x(00)00653-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000102997
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1016/s1074-7613(00)80167-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033920828
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1016/s1074-7613(00)80267-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010531915
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1073/pnas.77.5.2927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047299727
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1073/pnas.92.22.10302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032163309
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1073/pnas.96.14.8126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040869632
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1084/jem.185.7.1163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039547742
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1084/jem.186.4.619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010498871
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1093/emboj/19.11.2465 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006446860
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1111/j.1365-2141.1985.tb07393.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041931249
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1111/j.1432-0436.1986.tb00391.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050243033
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1126/science.8342023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062654735
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1182/blood.v99.4.1183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043466370
298 rdf:type schema:CreativeWork
299 https://www.grid.ac/institutes/grid.5645.2 schema:alternateName Erasmus University Medical Center
300 schema:name Cell Biology and Genetics, Erasmus Medical Center Rotterdam, The Netherlands
301 Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands
302 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...