Stromal cells from murine embryonic aorta–gonad–mesonephros region, liver and gut mesentery expand human umbilical cord blood-derived CAFCweek6 in extended long-term ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2002-09

AUTHORS

N Kusadasi, RAJ Oostendorp, WJLM Koevoet, EA Dzierzak, RE Ploemacher

ABSTRACT

The first definitive long-term repopulating hematopoietic stem cells (HSCs) emerge from and undergo rapid expansion in the embryonic aorta-gonad-mesonephros (AGM) region. To investigate the presumptive unique characteristics of the embryonic hematopoietic microenvironment and its surrounding tissues, we have generated stromal clones from subdissected day 10 and day 11 AGMs, embryonic livers (ELs) and gut mesentery. We here examine the ability of 19 of these clones to sustain extended long-term cultures (LTCs) of human CD34(+) umbilical cord blood (UCB) cells in vitro. The presence of in vitro repopulating cells was assessed by sustained production of progenitor cells (extended LTC-CFC) and cobblestone area-forming cells (CAFC). The embryonic stromal clones differed greatly in their support for human HSCs. Out of eight clones tested in the absence of exogenous cytokines, only one (EL-derived) clone was able to provide maintenance of HSCs. Addition of either Tpo or Flt3-L + Tpo improved the long-term support of about 50% of the tested clones. Cultures on four out of 19 clones, ie the EL-derived clone mentioned, two urogenital-ridge (UG)-derived clones and one gastrointestinal (GI)-derived clone, allowed a continuous expansion of primitive CAFC and CFU-GM with over several hundred-fold more CAFC(week6) produced in the 12th week of culture. This expansion was considerably higher than that found with the FBMD-1 cell line, which is appreciated by many investigators for its support of human HSCs, under comparable conditions. This stromal cell panel derived from the embryonic regions may be a powerful tool in dissecting the factors mediating stromal support for maintenance and expansion of HSCs. More... »

PAGES

1782

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.leu.2402615

DOI

http://dx.doi.org/10.1038/sj.leu.2402615

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047951257

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12200694


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD34", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aorta", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Lineage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Separation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clone Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coculture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colony-Forming Units Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytokines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Digestive System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo, Mammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fetal Blood", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gonads", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Graft Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hematopoiesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesentery", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesonephros", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Transgenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stromal Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kusadasi", 
        "givenName": "N", 
        "id": "sg:person.01244223270.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244223270.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Cell Biology and Genetics, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oostendorp", 
        "givenName": "RAJ", 
        "id": "sg:person.013304220014.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013304220014.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koevoet", 
        "givenName": "WJLM", 
        "id": "sg:person.01226564337.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226564337.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Cell Biology and Genetics, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dzierzak", 
        "givenName": "EA", 
        "id": "sg:person.01012155451.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012155451.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.5645.2", 
          "name": [
            "Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ploemacher", 
        "givenName": "RE", 
        "id": "sg:person.014316077474.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014316077474.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00653-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000102997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003126954", 
          "https://doi.org/10.1038/sj.leu.2401432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003126954", 
          "https://doi.org/10.1038/sj.leu.2401432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.11.2465", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006446860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2402204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010238804", 
          "https://doi.org/10.1038/sj.leu.2402204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2402204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010238804", 
          "https://doi.org/10.1038/sj.leu.2402204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.186.4.619", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010498871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80267-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010531915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00557-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010859962"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1041300121", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011977373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/382635a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016281241", 
          "https://doi.org/10.1038/382635a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017219884", 
          "https://doi.org/10.1038/sj.leu.2401003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017219884", 
          "https://doi.org/10.1038/sj.leu.2401003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80165-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023382146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.22.10302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032163309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80167-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033920828"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040910303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036776047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.185.7.1163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039547742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.14.8126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040869632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041729054", 
          "https://doi.org/10.1038/sj.leu.2401917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041729054", 
          "https://doi.org/10.1038/sj.leu.2401917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2141.1985.tb07393.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041931249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00515-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042892402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood.v99.4.1183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043466370"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.77.5.2927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047299727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1986.tb00391.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050243033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bmt.1702634", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050609506", 
          "https://doi.org/10.1038/sj.bmt.1702634"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8342023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062654735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074463122", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074601297", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074610541", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075039946", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079409629", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080072738", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082620066", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082889332", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083073897", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083105702", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083118290", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083274021", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083296922", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083314035", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083354363", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-09", 
    "datePublishedReg": "2002-09-01", 
    "description": "The first definitive long-term repopulating hematopoietic stem cells (HSCs) emerge from and undergo rapid expansion in the embryonic aorta-gonad-mesonephros (AGM) region. To investigate the presumptive unique characteristics of the embryonic hematopoietic microenvironment and its surrounding tissues, we have generated stromal clones from subdissected day 10 and day 11 AGMs, embryonic livers (ELs) and gut mesentery. We here examine the ability of 19 of these clones to sustain extended long-term cultures (LTCs) of human CD34(+) umbilical cord blood (UCB) cells in vitro. The presence of in vitro repopulating cells was assessed by sustained production of progenitor cells (extended LTC-CFC) and cobblestone area-forming cells (CAFC). The embryonic stromal clones differed greatly in their support for human HSCs. Out of eight clones tested in the absence of exogenous cytokines, only one (EL-derived) clone was able to provide maintenance of HSCs. Addition of either Tpo or Flt3-L + Tpo improved the long-term support of about 50% of the tested clones. Cultures on four out of 19 clones, ie the EL-derived clone mentioned, two urogenital-ridge (UG)-derived clones and one gastrointestinal (GI)-derived clone, allowed a continuous expansion of primitive CAFC and CFU-GM with over several hundred-fold more CAFC(week6) produced in the 12th week of culture. This expansion was considerably higher than that found with the FBMD-1 cell line, which is appreciated by many investigators for its support of human HSCs, under comparable conditions. This stromal cell panel derived from the embryonic regions may be a powerful tool in dissecting the factors mediating stromal support for maintenance and expansion of HSCs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.leu.2402615", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1097065", 
        "issn": [
          "0887-6924", 
          "1476-5551"
        ], 
        "name": "Leukemia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "16"
      }
    ], 
    "name": "Stromal cells from murine embryonic aorta\u2013gonad\u2013mesonephros region, liver and gut mesentery expand human umbilical cord blood-derived CAFCweek6 in extended long-term cultures", 
    "pagination": "1782", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e46dc2221816be281f584db1028021b45e5f3efb444950ad749a60bad2ec3ecc"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12200694"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8704895"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.leu.2402615"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1047951257"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.leu.2402615", 
      "https://app.dimensions.ai/details/publication/pub.1047951257"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29222_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/2402615"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402615'


 

This table displays all metadata directly associated to this object as RDF triples.

302 TRIPLES      21 PREDICATES      92 URIs      45 LITERALS      33 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.leu.2402615 schema:about N10851bb333c149eaa2e48406bcca0beb
2 N16c931a3a04644b4a817e0354a1b6462
3 N1cbe00c183c54476a05787c706b13662
4 N28824a2adcac446fb010806c12e2a2bf
5 N301576c4df9e4405bcc90425756c9842
6 N45fa18c88afc4365942627a1f705960a
7 N519f8d3a95724d62826c3c1aff5977da
8 N530b1637c55142c7bc6d7428e39b5b55
9 N5ea4cad187d4470a99ea2c8a145e0d25
10 N68eafe01904f4ca8a0e53ae9e64ee22f
11 N6ec3b99447a442768b7668092044b89c
12 N8b6c6843727840979fa85edda0722e40
13 N95a793fb8cb84c9e97a7e5e8a95ffc6c
14 Na218fe99dd034259a95350c6e9f48228
15 Na2a87d21d89f47c3b600faa1d9378277
16 Nae02e218bf75400bbb2ad3202be8cc89
17 Nb169cffe340f42678dfb5a179f779d6b
18 Nc5d4e2d18e8b4dda852862d96b5e6550
19 Ne27c3e20521a46869f02ad7157f84e92
20 Ne2abe1652bd74c04bf4d65c14ed128c4
21 Ne496dc031c9f4f74aa887255e525fd7d
22 Ne6da0a48063547eea082d75bb1f4f4b1
23 Nf05957250f3147138cf1e8b979d66f6b
24 Nfdc0be6d56c34e558ce5c66a5091f25a
25 anzsrc-for:06
26 anzsrc-for:0601
27 schema:author N180f9c20e5b04a0992025ca0f3c691bc
28 schema:citation sg:pub.10.1038/382635a0
29 sg:pub.10.1038/sj.bmt.1702634
30 sg:pub.10.1038/sj.leu.2401003
31 sg:pub.10.1038/sj.leu.2401432
32 sg:pub.10.1038/sj.leu.2401917
33 sg:pub.10.1038/sj.leu.2402204
34 https://app.dimensions.ai/details/publication/pub.1074463122
35 https://app.dimensions.ai/details/publication/pub.1074601297
36 https://app.dimensions.ai/details/publication/pub.1074610541
37 https://app.dimensions.ai/details/publication/pub.1075039946
38 https://app.dimensions.ai/details/publication/pub.1079409629
39 https://app.dimensions.ai/details/publication/pub.1080072738
40 https://app.dimensions.ai/details/publication/pub.1082620066
41 https://app.dimensions.ai/details/publication/pub.1082889332
42 https://app.dimensions.ai/details/publication/pub.1083073897
43 https://app.dimensions.ai/details/publication/pub.1083105702
44 https://app.dimensions.ai/details/publication/pub.1083118290
45 https://app.dimensions.ai/details/publication/pub.1083274021
46 https://app.dimensions.ai/details/publication/pub.1083296922
47 https://app.dimensions.ai/details/publication/pub.1083314035
48 https://app.dimensions.ai/details/publication/pub.1083354363
49 https://doi.org/10.1002/jcp.1040910303
50 https://doi.org/10.1002/jcp.1041300121
51 https://doi.org/10.1016/s0092-8674(00)80165-8
52 https://doi.org/10.1016/s0301-472x(00)00515-4
53 https://doi.org/10.1016/s0301-472x(00)00557-9
54 https://doi.org/10.1016/s0301-472x(00)00653-6
55 https://doi.org/10.1016/s1074-7613(00)80167-3
56 https://doi.org/10.1016/s1074-7613(00)80267-8
57 https://doi.org/10.1073/pnas.77.5.2927
58 https://doi.org/10.1073/pnas.92.22.10302
59 https://doi.org/10.1073/pnas.96.14.8126
60 https://doi.org/10.1084/jem.185.7.1163
61 https://doi.org/10.1084/jem.186.4.619
62 https://doi.org/10.1093/emboj/19.11.2465
63 https://doi.org/10.1111/j.1365-2141.1985.tb07393.x
64 https://doi.org/10.1111/j.1432-0436.1986.tb00391.x
65 https://doi.org/10.1126/science.8342023
66 https://doi.org/10.1182/blood.v99.4.1183
67 schema:datePublished 2002-09
68 schema:datePublishedReg 2002-09-01
69 schema:description The first definitive long-term repopulating hematopoietic stem cells (HSCs) emerge from and undergo rapid expansion in the embryonic aorta-gonad-mesonephros (AGM) region. To investigate the presumptive unique characteristics of the embryonic hematopoietic microenvironment and its surrounding tissues, we have generated stromal clones from subdissected day 10 and day 11 AGMs, embryonic livers (ELs) and gut mesentery. We here examine the ability of 19 of these clones to sustain extended long-term cultures (LTCs) of human CD34(+) umbilical cord blood (UCB) cells in vitro. The presence of in vitro repopulating cells was assessed by sustained production of progenitor cells (extended LTC-CFC) and cobblestone area-forming cells (CAFC). The embryonic stromal clones differed greatly in their support for human HSCs. Out of eight clones tested in the absence of exogenous cytokines, only one (EL-derived) clone was able to provide maintenance of HSCs. Addition of either Tpo or Flt3-L + Tpo improved the long-term support of about 50% of the tested clones. Cultures on four out of 19 clones, ie the EL-derived clone mentioned, two urogenital-ridge (UG)-derived clones and one gastrointestinal (GI)-derived clone, allowed a continuous expansion of primitive CAFC and CFU-GM with over several hundred-fold more CAFC(week6) produced in the 12th week of culture. This expansion was considerably higher than that found with the FBMD-1 cell line, which is appreciated by many investigators for its support of human HSCs, under comparable conditions. This stromal cell panel derived from the embryonic regions may be a powerful tool in dissecting the factors mediating stromal support for maintenance and expansion of HSCs.
70 schema:genre research_article
71 schema:inLanguage en
72 schema:isAccessibleForFree true
73 schema:isPartOf N4400f0f105524628b926d3060e2e61ac
74 N4707ce2a8cb7463abf7b7dda780bb873
75 sg:journal.1097065
76 schema:name Stromal cells from murine embryonic aorta–gonad–mesonephros region, liver and gut mesentery expand human umbilical cord blood-derived CAFCweek6 in extended long-term cultures
77 schema:pagination 1782
78 schema:productId N03e70e023ce04deab00aae765caa97cf
79 N06b4458c90a9421d9be551dcb8724ac0
80 N1e170bb9c2364cefb49c24551f52b81e
81 N8dbec262eb61402d91409b993251b527
82 Nde8e014e8fbe4b25b886315d2887ed52
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047951257
84 https://doi.org/10.1038/sj.leu.2402615
85 schema:sdDatePublished 2019-04-11T11:58
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher Nbcd7872ae684463883a068c932777ce8
88 schema:url https://www.nature.com/articles/2402615
89 sgo:license sg:explorer/license/
90 sgo:sdDataset articles
91 rdf:type schema:ScholarlyArticle
92 N03e70e023ce04deab00aae765caa97cf schema:name readcube_id
93 schema:value e46dc2221816be281f584db1028021b45e5f3efb444950ad749a60bad2ec3ecc
94 rdf:type schema:PropertyValue
95 N06b4458c90a9421d9be551dcb8724ac0 schema:name nlm_unique_id
96 schema:value 8704895
97 rdf:type schema:PropertyValue
98 N10851bb333c149eaa2e48406bcca0beb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Aorta
100 rdf:type schema:DefinedTerm
101 N16c931a3a04644b4a817e0354a1b6462 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Digestive System
103 rdf:type schema:DefinedTerm
104 N180f9c20e5b04a0992025ca0f3c691bc rdf:first sg:person.01244223270.13
105 rdf:rest Na7c595ded7314e8b8d90bb5e466edcb0
106 N1cbe00c183c54476a05787c706b13662 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Stromal Cells
108 rdf:type schema:DefinedTerm
109 N1e170bb9c2364cefb49c24551f52b81e schema:name dimensions_id
110 schema:value pub.1047951257
111 rdf:type schema:PropertyValue
112 N28824a2adcac446fb010806c12e2a2bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Humans
114 rdf:type schema:DefinedTerm
115 N301576c4df9e4405bcc90425756c9842 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Cell Lineage
117 rdf:type schema:DefinedTerm
118 N4400f0f105524628b926d3060e2e61ac schema:volumeNumber 16
119 rdf:type schema:PublicationVolume
120 N45fa18c88afc4365942627a1f705960a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Animals
122 rdf:type schema:DefinedTerm
123 N4707ce2a8cb7463abf7b7dda780bb873 schema:issueNumber 9
124 rdf:type schema:PublicationIssue
125 N519f8d3a95724d62826c3c1aff5977da schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Mice, Transgenic
127 rdf:type schema:DefinedTerm
128 N530b1637c55142c7bc6d7428e39b5b55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Mice
130 rdf:type schema:DefinedTerm
131 N5e2f3502585149ecb931cc3f99dd39f2 rdf:first sg:person.01226564337.27
132 rdf:rest N8fe06eb7f6744aabbc4f12c5e4115dda
133 N5ea4cad187d4470a99ea2c8a145e0d25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Cell Separation
135 rdf:type schema:DefinedTerm
136 N68eafe01904f4ca8a0e53ae9e64ee22f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Liver
138 rdf:type schema:DefinedTerm
139 N6ec3b99447a442768b7668092044b89c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Mesonephros
141 rdf:type schema:DefinedTerm
142 N8b6c6843727840979fa85edda0722e40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Fetal Blood
144 rdf:type schema:DefinedTerm
145 N8dbec262eb61402d91409b993251b527 schema:name pubmed_id
146 schema:value 12200694
147 rdf:type schema:PropertyValue
148 N8fe06eb7f6744aabbc4f12c5e4115dda rdf:first sg:person.01012155451.06
149 rdf:rest Ne301e4d61849462d873030946e4eae4f
150 N95a793fb8cb84c9e97a7e5e8a95ffc6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Cytokines
152 rdf:type schema:DefinedTerm
153 Na218fe99dd034259a95350c6e9f48228 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Graft Survival
155 rdf:type schema:DefinedTerm
156 Na2a87d21d89f47c3b600faa1d9378277 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Reverse Transcriptase Polymerase Chain Reaction
158 rdf:type schema:DefinedTerm
159 Na7c595ded7314e8b8d90bb5e466edcb0 rdf:first sg:person.013304220014.01
160 rdf:rest N5e2f3502585149ecb931cc3f99dd39f2
161 Nae02e218bf75400bbb2ad3202be8cc89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Stem Cells
163 rdf:type schema:DefinedTerm
164 Nb169cffe340f42678dfb5a179f779d6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Gonads
166 rdf:type schema:DefinedTerm
167 Nbcd7872ae684463883a068c932777ce8 schema:name Springer Nature - SN SciGraph project
168 rdf:type schema:Organization
169 Nc5d4e2d18e8b4dda852862d96b5e6550 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Clone Cells
171 rdf:type schema:DefinedTerm
172 Nde8e014e8fbe4b25b886315d2887ed52 schema:name doi
173 schema:value 10.1038/sj.leu.2402615
174 rdf:type schema:PropertyValue
175 Ne27c3e20521a46869f02ad7157f84e92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Coculture Techniques
177 rdf:type schema:DefinedTerm
178 Ne2abe1652bd74c04bf4d65c14ed128c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Hematopoiesis
180 rdf:type schema:DefinedTerm
181 Ne301e4d61849462d873030946e4eae4f rdf:first sg:person.014316077474.52
182 rdf:rest rdf:nil
183 Ne496dc031c9f4f74aa887255e525fd7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Colony-Forming Units Assay
185 rdf:type schema:DefinedTerm
186 Ne6da0a48063547eea082d75bb1f4f4b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Embryo, Mammalian
188 rdf:type schema:DefinedTerm
189 Nf05957250f3147138cf1e8b979d66f6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
190 schema:name Antigens, CD34
191 rdf:type schema:DefinedTerm
192 Nfdc0be6d56c34e558ce5c66a5091f25a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Mesentery
194 rdf:type schema:DefinedTerm
195 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
196 schema:name Biological Sciences
197 rdf:type schema:DefinedTerm
198 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
199 schema:name Biochemistry and Cell Biology
200 rdf:type schema:DefinedTerm
201 sg:journal.1097065 schema:issn 0887-6924
202 1476-5551
203 schema:name Leukemia
204 rdf:type schema:Periodical
205 sg:person.01012155451.06 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
206 schema:familyName Dzierzak
207 schema:givenName EA
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012155451.06
209 rdf:type schema:Person
210 sg:person.01226564337.27 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
211 schema:familyName Koevoet
212 schema:givenName WJLM
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226564337.27
214 rdf:type schema:Person
215 sg:person.01244223270.13 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
216 schema:familyName Kusadasi
217 schema:givenName N
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244223270.13
219 rdf:type schema:Person
220 sg:person.013304220014.01 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
221 schema:familyName Oostendorp
222 schema:givenName RAJ
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013304220014.01
224 rdf:type schema:Person
225 sg:person.014316077474.52 schema:affiliation https://www.grid.ac/institutes/grid.5645.2
226 schema:familyName Ploemacher
227 schema:givenName RE
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014316077474.52
229 rdf:type schema:Person
230 sg:pub.10.1038/382635a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016281241
231 https://doi.org/10.1038/382635a0
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/sj.bmt.1702634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050609506
234 https://doi.org/10.1038/sj.bmt.1702634
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/sj.leu.2401003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017219884
237 https://doi.org/10.1038/sj.leu.2401003
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/sj.leu.2401432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003126954
240 https://doi.org/10.1038/sj.leu.2401432
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/sj.leu.2401917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041729054
243 https://doi.org/10.1038/sj.leu.2401917
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/sj.leu.2402204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010238804
246 https://doi.org/10.1038/sj.leu.2402204
247 rdf:type schema:CreativeWork
248 https://app.dimensions.ai/details/publication/pub.1074463122 schema:CreativeWork
249 https://app.dimensions.ai/details/publication/pub.1074601297 schema:CreativeWork
250 https://app.dimensions.ai/details/publication/pub.1074610541 schema:CreativeWork
251 https://app.dimensions.ai/details/publication/pub.1075039946 schema:CreativeWork
252 https://app.dimensions.ai/details/publication/pub.1079409629 schema:CreativeWork
253 https://app.dimensions.ai/details/publication/pub.1080072738 schema:CreativeWork
254 https://app.dimensions.ai/details/publication/pub.1082620066 schema:CreativeWork
255 https://app.dimensions.ai/details/publication/pub.1082889332 schema:CreativeWork
256 https://app.dimensions.ai/details/publication/pub.1083073897 schema:CreativeWork
257 https://app.dimensions.ai/details/publication/pub.1083105702 schema:CreativeWork
258 https://app.dimensions.ai/details/publication/pub.1083118290 schema:CreativeWork
259 https://app.dimensions.ai/details/publication/pub.1083274021 schema:CreativeWork
260 https://app.dimensions.ai/details/publication/pub.1083296922 schema:CreativeWork
261 https://app.dimensions.ai/details/publication/pub.1083314035 schema:CreativeWork
262 https://app.dimensions.ai/details/publication/pub.1083354363 schema:CreativeWork
263 https://doi.org/10.1002/jcp.1040910303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036776047
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1002/jcp.1041300121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011977373
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/s0092-8674(00)80165-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023382146
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/s0301-472x(00)00515-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042892402
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1016/s0301-472x(00)00557-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010859962
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1016/s0301-472x(00)00653-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000102997
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1016/s1074-7613(00)80167-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033920828
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1016/s1074-7613(00)80267-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010531915
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1073/pnas.77.5.2927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047299727
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1073/pnas.92.22.10302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032163309
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1073/pnas.96.14.8126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040869632
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1084/jem.185.7.1163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039547742
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1084/jem.186.4.619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010498871
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1093/emboj/19.11.2465 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006446860
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1111/j.1365-2141.1985.tb07393.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041931249
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1111/j.1432-0436.1986.tb00391.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050243033
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1126/science.8342023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062654735
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1182/blood.v99.4.1183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043466370
298 rdf:type schema:CreativeWork
299 https://www.grid.ac/institutes/grid.5645.2 schema:alternateName Erasmus University Medical Center
300 schema:name Cell Biology and Genetics, Erasmus Medical Center Rotterdam, The Netherlands
301 Institute of Hematology, Erasmus Medical Center Rotterdam, The Netherlands
302 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...