Stromal support augments extended long-term ex vivo expansion of hemopoietic progenitor cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2001-09

AUTHORS

N Kusadasi, JLM Koevoet, PL van Soest, RE Ploemacher

ABSTRACT

Current technology to numerically expand hemopoietic stem/progenitor cells (HSPC) ex vivo within 1 to 2 weeks is insufficient to warrant significant gain in reconstitution time following their transplantation. In order to more stringently test the parameters affecting HSPC expansion, we followed ex vivo cultures of CD34+-selected umbilical cord blood (UCB) HSPC for up to 10 weeks and investigated the effects of stromal support and cytokine addition. The cytokine combinations included FL + TPO, FL + TPO plus SCF and/or IL6, or SCF + IL6. To identify the HSPC in uncultured and cultured material, we determined the number of colony-forming cells (CFC), cobblestone area forming cells (CAFC), the NOD/SCID repopulating ability (SRA), and CD34+ subsets by phenotyping. The highest fold-increase obtained for CD34+ and CD34+ CD38- cell numbers was, respectively, 1197 and 30,937 for stroma-free and 4066 and 117,235 for stroma-supported cultures. In general, CFC generation increased weekly in FL + TPO containing groups up to week 5 with a 28- to 195-fold expansion whereafter the weekly CFC output stabilized. Stroma support enhanced the expansion of CAFC week 6 maximally 11-fold to 89-fold with FL + TPO + IL6. Cultures stimulated with at least FL + TPO gave an estimated 10- to 14-fold expansion of the ability of CD34+ UCB cells to multilineage engraft the BM of sublethally irradiated NOD/SCID mice at 2 weeks of stroma-free and stroma-supported cultures, while at week 5 and later the estimated SRA decreased to low or undetectable levels in all groups. Our results show that stroma and FL + TPO but also inclusion of bovine serum albumin, greatly increase the long-term generation of HSPC as measured by in vitro assays and is indispensable for long-term expansion of CD34+ CD38- CXCR4+ cells. However, the different surrogate methods to quantify the HSPC (CD34+ CD38-, CFC, CAFC week 6 and SRA) show increasing incongruency with increasing culture time, while especially the phenotypic analysis and the CFC generation greatly overestimate the CAFC and SRA expansion in 10-week cultures. More... »

PAGES

1347

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.leu.2402204

DOI

http://dx.doi.org/10.1038/sj.leu.2402204

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010238804

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11516095


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "ADP-ribosyl Cyclase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "ADP-ribosyl Cyclase 1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD34", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytokines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hematopoietic Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interleukin-6", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Glycoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred NOD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, SCID", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "NAD+ Nucleosidase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serum Albumin, Bovine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cell Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thrombopoietin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transforming Growth Factor beta", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Erasmus University Rotterdam", 
          "id": "https://www.grid.ac/institutes/grid.6906.9", 
          "name": [
            "Institute of Hematology, Erasmus University Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kusadasi", 
        "givenName": "N", 
        "id": "sg:person.01244223270.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244223270.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Rotterdam", 
          "id": "https://www.grid.ac/institutes/grid.6906.9", 
          "name": [
            "Institute of Hematology, Erasmus University Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koevoet", 
        "givenName": "JLM", 
        "id": "sg:person.0625471571.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625471571.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Rotterdam", 
          "id": "https://www.grid.ac/institutes/grid.6906.9", 
          "name": [
            "Institute of Hematology, Erasmus University Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van Soest", 
        "givenName": "PL", 
        "id": "sg:person.0736072206.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736072206.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Erasmus University Rotterdam", 
          "id": "https://www.grid.ac/institutes/grid.6906.9", 
          "name": [
            "Institute of Hematology, Erasmus University Rotterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ploemacher", 
        "givenName": "RE", 
        "id": "sg:person.014316077474.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014316077474.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.97.26.14626", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002642815"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.145.6.1612", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004973707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.186.4.619", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010498871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2141.1999.01677.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015105993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/382635a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016281241", 
          "https://doi.org/10.1038/382635a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017219884", 
          "https://doi.org/10.1038/sj.leu.2401003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017219884", 
          "https://doi.org/10.1038/sj.leu.2401003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(99)00012-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019668782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020131474", 
          "https://doi.org/10.1038/sj.leu.2401740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020131474", 
          "https://doi.org/10.1038/sj.leu.2401740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0994-909", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022671474", 
          "https://doi.org/10.1038/nbt0994-909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.5530130610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025735562"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.5530130610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025735562"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.4.1470", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029011698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00154-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031333805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00177-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038664403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(99)00037-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039068293"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401234", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039332685", 
          "https://doi.org/10.1038/sj.leu.2401234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401234", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039332685", 
          "https://doi.org/10.1038/sj.leu.2401234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.14.8126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040869632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041729054", 
          "https://doi.org/10.1038/sj.leu.2401917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2401917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041729054", 
          "https://doi.org/10.1038/sj.leu.2401917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci8583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042806918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(00)00515-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042892402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2400530", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045668759", 
          "https://doi.org/10.1038/sj.leu.2400530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2400530", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045668759", 
          "https://doi.org/10.1038/sj.leu.2400530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040900113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050872340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.283.5403.845", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062564103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8342023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062654735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074463122", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074517536", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074559945", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074566060", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074566065", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074601297", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074666162", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077333563", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079409629", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082452236", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082467296", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082759788", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082826342", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082889332", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082889390", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082912972", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082990689", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083012811", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083056491", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083073897", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083118290", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083152189", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083191040", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083197280", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083207387", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083274021", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083288555", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083313262", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083314035", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083335824", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2001-09", 
    "datePublishedReg": "2001-09-01", 
    "description": "Current technology to numerically expand hemopoietic stem/progenitor cells (HSPC) ex vivo within 1 to 2 weeks is insufficient to warrant significant gain in reconstitution time following their transplantation. In order to more stringently test the parameters affecting HSPC expansion, we followed ex vivo cultures of CD34+-selected umbilical cord blood (UCB) HSPC for up to 10 weeks and investigated the effects of stromal support and cytokine addition. The cytokine combinations included FL + TPO, FL + TPO plus SCF and/or IL6, or SCF + IL6. To identify the HSPC in uncultured and cultured material, we determined the number of colony-forming cells (CFC), cobblestone area forming cells (CAFC), the NOD/SCID repopulating ability (SRA), and CD34+ subsets by phenotyping. The highest fold-increase obtained for CD34+ and CD34+ CD38- cell numbers was, respectively, 1197 and 30,937 for stroma-free and 4066 and 117,235 for stroma-supported cultures. In general, CFC generation increased weekly in FL + TPO containing groups up to week 5 with a 28- to 195-fold expansion whereafter the weekly CFC output stabilized. Stroma support enhanced the expansion of CAFC week 6 maximally 11-fold to 89-fold with FL + TPO + IL6. Cultures stimulated with at least FL + TPO gave an estimated 10- to 14-fold expansion of the ability of CD34+ UCB cells to multilineage engraft the BM of sublethally irradiated NOD/SCID mice at 2 weeks of stroma-free and stroma-supported cultures, while at week 5 and later the estimated SRA decreased to low or undetectable levels in all groups. Our results show that stroma and FL + TPO but also inclusion of bovine serum albumin, greatly increase the long-term generation of HSPC as measured by in vitro assays and is indispensable for long-term expansion of CD34+ CD38- CXCR4+ cells. However, the different surrogate methods to quantify the HSPC (CD34+ CD38-, CFC, CAFC week 6 and SRA) show increasing incongruency with increasing culture time, while especially the phenotypic analysis and the CFC generation greatly overestimate the CAFC and SRA expansion in 10-week cultures.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.leu.2402204", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1097065", 
        "issn": [
          "0887-6924", 
          "1476-5551"
        ], 
        "name": "Leukemia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Stromal support augments extended long-term ex vivo expansion of hemopoietic progenitor cells", 
    "pagination": "1347", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "30c73c9fda910de5f6ec42e45f9ecd94642f180c21c1e82cee2edea8138a9474"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11516095"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8704895"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.leu.2402204"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010238804"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.leu.2402204", 
      "https://app.dimensions.ai/details/publication/pub.1010238804"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29212_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/2402204"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402204'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402204'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402204'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2402204'


 

This table displays all metadata directly associated to this object as RDF triples.

318 TRIPLES      21 PREDICATES      105 URIs      44 LITERALS      32 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.leu.2402204 schema:about N0ecd3d8e5864458db7e37bd1348b2da0
2 N11aaa57092564362bad3921fe7e6eca3
3 N21107c83877a492e8375cc599784279e
4 N2fe3d44a1516476b9feaf3ee39cdd698
5 N327c34e232734b23aed3dde605e4bb3b
6 N3664454d0674484bbe2250c50010ea8c
7 N560e9db53cb64f2cb72ddd91a959c60d
8 N58a9ebfe963d4225b93fc7300eaa96b4
9 N5a32334086c5415182aa84210d949989
10 N5e7c75de108c43ffaccfdb64421662bd
11 N793d8fdb8ffa4279834333205537d59a
12 N7dbdac817815461588c0353b5780f8c4
13 N90658588bad24cc5a677bf0047d11bb2
14 N975f943a4ab048778b638f0eba610a89
15 N9b2b43b328274f6993e74c75916f0555
16 N9eff9ba2a0124adbbd216095ffe54411
17 Nb7f7f7bed08644918ed221ce33ceec35
18 Nc02ffba3fd434f59b88f3cb8aa1781bb
19 Nc48aa06d80434c9ebff12111125b39a1
20 Nc940b7737a2d494580c387cd1ef2e371
21 Ncfc5717d28794d2d9ce5a679e7b56bfe
22 Nf07c97d3255f4b68b3d29a4aecd47ed4
23 Nffc48fff62d9458283a3f0e28e70fcb4
24 anzsrc-for:11
25 anzsrc-for:1103
26 schema:author N434aa1768d1c436aa8927b847bd31f5a
27 schema:citation sg:pub.10.1038/382635a0
28 sg:pub.10.1038/nbt0994-909
29 sg:pub.10.1038/sj.leu.2400530
30 sg:pub.10.1038/sj.leu.2401003
31 sg:pub.10.1038/sj.leu.2401234
32 sg:pub.10.1038/sj.leu.2401740
33 sg:pub.10.1038/sj.leu.2401917
34 https://app.dimensions.ai/details/publication/pub.1074463122
35 https://app.dimensions.ai/details/publication/pub.1074517536
36 https://app.dimensions.ai/details/publication/pub.1074559945
37 https://app.dimensions.ai/details/publication/pub.1074566060
38 https://app.dimensions.ai/details/publication/pub.1074566065
39 https://app.dimensions.ai/details/publication/pub.1074601297
40 https://app.dimensions.ai/details/publication/pub.1074666162
41 https://app.dimensions.ai/details/publication/pub.1077333563
42 https://app.dimensions.ai/details/publication/pub.1079409629
43 https://app.dimensions.ai/details/publication/pub.1082452236
44 https://app.dimensions.ai/details/publication/pub.1082467296
45 https://app.dimensions.ai/details/publication/pub.1082759788
46 https://app.dimensions.ai/details/publication/pub.1082826342
47 https://app.dimensions.ai/details/publication/pub.1082889332
48 https://app.dimensions.ai/details/publication/pub.1082889390
49 https://app.dimensions.ai/details/publication/pub.1082912972
50 https://app.dimensions.ai/details/publication/pub.1082990689
51 https://app.dimensions.ai/details/publication/pub.1083012811
52 https://app.dimensions.ai/details/publication/pub.1083056491
53 https://app.dimensions.ai/details/publication/pub.1083073897
54 https://app.dimensions.ai/details/publication/pub.1083118290
55 https://app.dimensions.ai/details/publication/pub.1083152189
56 https://app.dimensions.ai/details/publication/pub.1083191040
57 https://app.dimensions.ai/details/publication/pub.1083197280
58 https://app.dimensions.ai/details/publication/pub.1083207387
59 https://app.dimensions.ai/details/publication/pub.1083274021
60 https://app.dimensions.ai/details/publication/pub.1083288555
61 https://app.dimensions.ai/details/publication/pub.1083313262
62 https://app.dimensions.ai/details/publication/pub.1083314035
63 https://app.dimensions.ai/details/publication/pub.1083335824
64 https://doi.org/10.1002/jcp.1040900113
65 https://doi.org/10.1002/stem.5530130610
66 https://doi.org/10.1016/s0301-472x(00)00154-5
67 https://doi.org/10.1016/s0301-472x(00)00177-6
68 https://doi.org/10.1016/s0301-472x(00)00515-4
69 https://doi.org/10.1016/s0301-472x(99)00012-0
70 https://doi.org/10.1016/s0301-472x(99)00037-5
71 https://doi.org/10.1046/j.1365-2141.1999.01677.x
72 https://doi.org/10.1073/pnas.93.4.1470
73 https://doi.org/10.1073/pnas.96.14.8126
74 https://doi.org/10.1073/pnas.97.26.14626
75 https://doi.org/10.1084/jem.145.6.1612
76 https://doi.org/10.1084/jem.186.4.619
77 https://doi.org/10.1126/science.283.5403.845
78 https://doi.org/10.1126/science.8342023
79 https://doi.org/10.1172/jci8583
80 schema:datePublished 2001-09
81 schema:datePublishedReg 2001-09-01
82 schema:description Current technology to numerically expand hemopoietic stem/progenitor cells (HSPC) ex vivo within 1 to 2 weeks is insufficient to warrant significant gain in reconstitution time following their transplantation. In order to more stringently test the parameters affecting HSPC expansion, we followed ex vivo cultures of CD34+-selected umbilical cord blood (UCB) HSPC for up to 10 weeks and investigated the effects of stromal support and cytokine addition. The cytokine combinations included FL + TPO, FL + TPO plus SCF and/or IL6, or SCF + IL6. To identify the HSPC in uncultured and cultured material, we determined the number of colony-forming cells (CFC), cobblestone area forming cells (CAFC), the NOD/SCID repopulating ability (SRA), and CD34+ subsets by phenotyping. The highest fold-increase obtained for CD34+ and CD34+ CD38- cell numbers was, respectively, 1197 and 30,937 for stroma-free and 4066 and 117,235 for stroma-supported cultures. In general, CFC generation increased weekly in FL + TPO containing groups up to week 5 with a 28- to 195-fold expansion whereafter the weekly CFC output stabilized. Stroma support enhanced the expansion of CAFC week 6 maximally 11-fold to 89-fold with FL + TPO + IL6. Cultures stimulated with at least FL + TPO gave an estimated 10- to 14-fold expansion of the ability of CD34+ UCB cells to multilineage engraft the BM of sublethally irradiated NOD/SCID mice at 2 weeks of stroma-free and stroma-supported cultures, while at week 5 and later the estimated SRA decreased to low or undetectable levels in all groups. Our results show that stroma and FL + TPO but also inclusion of bovine serum albumin, greatly increase the long-term generation of HSPC as measured by in vitro assays and is indispensable for long-term expansion of CD34+ CD38- CXCR4+ cells. However, the different surrogate methods to quantify the HSPC (CD34+ CD38-, CFC, CAFC week 6 and SRA) show increasing incongruency with increasing culture time, while especially the phenotypic analysis and the CFC generation greatly overestimate the CAFC and SRA expansion in 10-week cultures.
83 schema:genre research_article
84 schema:inLanguage en
85 schema:isAccessibleForFree true
86 schema:isPartOf N110ec7a215554977aaef6f46598e8084
87 N2b11256a5099458890eb8dad541cc920
88 sg:journal.1097065
89 schema:name Stromal support augments extended long-term ex vivo expansion of hemopoietic progenitor cells
90 schema:pagination 1347
91 schema:productId N246ff05baff84c1cb83dc40a51cf1477
92 N6c00c306e0534d32b9a634e2128ad6dd
93 N776ea82d3d5344dc809a026e0ead0201
94 Nea098307da7f49cf867505b2a2cefc95
95 Nf5b820f61dee40e2960c64bbd0cb20ed
96 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010238804
97 https://doi.org/10.1038/sj.leu.2402204
98 schema:sdDatePublished 2019-04-11T11:55
99 schema:sdLicense https://scigraph.springernature.com/explorer/license/
100 schema:sdPublisher N178486ef950f4597a472ececfe563d0a
101 schema:url https://www.nature.com/articles/2402204
102 sgo:license sg:explorer/license/
103 sgo:sdDataset articles
104 rdf:type schema:ScholarlyArticle
105 N0ecd3d8e5864458db7e37bd1348b2da0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Hematopoietic Stem Cells
107 rdf:type schema:DefinedTerm
108 N110ec7a215554977aaef6f46598e8084 schema:volumeNumber 15
109 rdf:type schema:PublicationVolume
110 N11aaa57092564362bad3921fe7e6eca3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Antigens, Differentiation
112 rdf:type schema:DefinedTerm
113 N178486ef950f4597a472ececfe563d0a schema:name Springer Nature - SN SciGraph project
114 rdf:type schema:Organization
115 N21107c83877a492e8375cc599784279e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name ADP-ribosyl Cyclase 1
117 rdf:type schema:DefinedTerm
118 N246ff05baff84c1cb83dc40a51cf1477 schema:name doi
119 schema:value 10.1038/sj.leu.2402204
120 rdf:type schema:PropertyValue
121 N2b11256a5099458890eb8dad541cc920 schema:issueNumber 9
122 rdf:type schema:PublicationIssue
123 N2fe3d44a1516476b9feaf3ee39cdd698 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Animals
125 rdf:type schema:DefinedTerm
126 N327c34e232734b23aed3dde605e4bb3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Antigens, CD34
128 rdf:type schema:DefinedTerm
129 N3664454d0674484bbe2250c50010ea8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Mice
131 rdf:type schema:DefinedTerm
132 N434aa1768d1c436aa8927b847bd31f5a rdf:first sg:person.01244223270.13
133 rdf:rest Na44539369c164cd59f02d62e0447ee81
134 N560e9db53cb64f2cb72ddd91a959c60d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Membrane Glycoproteins
136 rdf:type schema:DefinedTerm
137 N58a9ebfe963d4225b93fc7300eaa96b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Stem Cell Factor
139 rdf:type schema:DefinedTerm
140 N5a32334086c5415182aa84210d949989 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Time Factors
142 rdf:type schema:DefinedTerm
143 N5e7c75de108c43ffaccfdb64421662bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Phenotype
145 rdf:type schema:DefinedTerm
146 N6bb5da118e5e44f2b9c0aef5ec01e0f4 rdf:first sg:person.014316077474.52
147 rdf:rest rdf:nil
148 N6c00c306e0534d32b9a634e2128ad6dd schema:name nlm_unique_id
149 schema:value 8704895
150 rdf:type schema:PropertyValue
151 N746d9f552e4e4236b1a0b4898c15ee35 rdf:first sg:person.0736072206.96
152 rdf:rest N6bb5da118e5e44f2b9c0aef5ec01e0f4
153 N776ea82d3d5344dc809a026e0ead0201 schema:name readcube_id
154 schema:value 30c73c9fda910de5f6ec42e45f9ecd94642f180c21c1e82cee2edea8138a9474
155 rdf:type schema:PropertyValue
156 N793d8fdb8ffa4279834333205537d59a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Mice, SCID
158 rdf:type schema:DefinedTerm
159 N7dbdac817815461588c0353b5780f8c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Interleukin-6
161 rdf:type schema:DefinedTerm
162 N90658588bad24cc5a677bf0047d11bb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Membrane Proteins
164 rdf:type schema:DefinedTerm
165 N975f943a4ab048778b638f0eba610a89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name ADP-ribosyl Cyclase
167 rdf:type schema:DefinedTerm
168 N9b2b43b328274f6993e74c75916f0555 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Cytokines
170 rdf:type schema:DefinedTerm
171 N9eff9ba2a0124adbbd216095ffe54411 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Mice, Inbred NOD
173 rdf:type schema:DefinedTerm
174 Na44539369c164cd59f02d62e0447ee81 rdf:first sg:person.0625471571.36
175 rdf:rest N746d9f552e4e4236b1a0b4898c15ee35
176 Nb7f7f7bed08644918ed221ce33ceec35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Transforming Growth Factor beta
178 rdf:type schema:DefinedTerm
179 Nc02ffba3fd434f59b88f3cb8aa1781bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Antigens, CD
181 rdf:type schema:DefinedTerm
182 Nc48aa06d80434c9ebff12111125b39a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Serum Albumin, Bovine
184 rdf:type schema:DefinedTerm
185 Nc940b7737a2d494580c387cd1ef2e371 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name NAD+ Nucleosidase
187 rdf:type schema:DefinedTerm
188 Ncfc5717d28794d2d9ce5a679e7b56bfe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Cell Culture Techniques
190 rdf:type schema:DefinedTerm
191 Nea098307da7f49cf867505b2a2cefc95 schema:name pubmed_id
192 schema:value 11516095
193 rdf:type schema:PropertyValue
194 Nf07c97d3255f4b68b3d29a4aecd47ed4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Thrombopoietin
196 rdf:type schema:DefinedTerm
197 Nf5b820f61dee40e2960c64bbd0cb20ed schema:name dimensions_id
198 schema:value pub.1010238804
199 rdf:type schema:PropertyValue
200 Nffc48fff62d9458283a3f0e28e70fcb4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
201 schema:name Humans
202 rdf:type schema:DefinedTerm
203 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
204 schema:name Medical and Health Sciences
205 rdf:type schema:DefinedTerm
206 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
207 schema:name Clinical Sciences
208 rdf:type schema:DefinedTerm
209 sg:journal.1097065 schema:issn 0887-6924
210 1476-5551
211 schema:name Leukemia
212 rdf:type schema:Periodical
213 sg:person.01244223270.13 schema:affiliation https://www.grid.ac/institutes/grid.6906.9
214 schema:familyName Kusadasi
215 schema:givenName N
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244223270.13
217 rdf:type schema:Person
218 sg:person.014316077474.52 schema:affiliation https://www.grid.ac/institutes/grid.6906.9
219 schema:familyName Ploemacher
220 schema:givenName RE
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014316077474.52
222 rdf:type schema:Person
223 sg:person.0625471571.36 schema:affiliation https://www.grid.ac/institutes/grid.6906.9
224 schema:familyName Koevoet
225 schema:givenName JLM
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625471571.36
227 rdf:type schema:Person
228 sg:person.0736072206.96 schema:affiliation https://www.grid.ac/institutes/grid.6906.9
229 schema:familyName van Soest
230 schema:givenName PL
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736072206.96
232 rdf:type schema:Person
233 sg:pub.10.1038/382635a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016281241
234 https://doi.org/10.1038/382635a0
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/nbt0994-909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022671474
237 https://doi.org/10.1038/nbt0994-909
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/sj.leu.2400530 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045668759
240 https://doi.org/10.1038/sj.leu.2400530
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/sj.leu.2401003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017219884
243 https://doi.org/10.1038/sj.leu.2401003
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/sj.leu.2401234 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039332685
246 https://doi.org/10.1038/sj.leu.2401234
247 rdf:type schema:CreativeWork
248 sg:pub.10.1038/sj.leu.2401740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020131474
249 https://doi.org/10.1038/sj.leu.2401740
250 rdf:type schema:CreativeWork
251 sg:pub.10.1038/sj.leu.2401917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041729054
252 https://doi.org/10.1038/sj.leu.2401917
253 rdf:type schema:CreativeWork
254 https://app.dimensions.ai/details/publication/pub.1074463122 schema:CreativeWork
255 https://app.dimensions.ai/details/publication/pub.1074517536 schema:CreativeWork
256 https://app.dimensions.ai/details/publication/pub.1074559945 schema:CreativeWork
257 https://app.dimensions.ai/details/publication/pub.1074566060 schema:CreativeWork
258 https://app.dimensions.ai/details/publication/pub.1074566065 schema:CreativeWork
259 https://app.dimensions.ai/details/publication/pub.1074601297 schema:CreativeWork
260 https://app.dimensions.ai/details/publication/pub.1074666162 schema:CreativeWork
261 https://app.dimensions.ai/details/publication/pub.1077333563 schema:CreativeWork
262 https://app.dimensions.ai/details/publication/pub.1079409629 schema:CreativeWork
263 https://app.dimensions.ai/details/publication/pub.1082452236 schema:CreativeWork
264 https://app.dimensions.ai/details/publication/pub.1082467296 schema:CreativeWork
265 https://app.dimensions.ai/details/publication/pub.1082759788 schema:CreativeWork
266 https://app.dimensions.ai/details/publication/pub.1082826342 schema:CreativeWork
267 https://app.dimensions.ai/details/publication/pub.1082889332 schema:CreativeWork
268 https://app.dimensions.ai/details/publication/pub.1082889390 schema:CreativeWork
269 https://app.dimensions.ai/details/publication/pub.1082912972 schema:CreativeWork
270 https://app.dimensions.ai/details/publication/pub.1082990689 schema:CreativeWork
271 https://app.dimensions.ai/details/publication/pub.1083012811 schema:CreativeWork
272 https://app.dimensions.ai/details/publication/pub.1083056491 schema:CreativeWork
273 https://app.dimensions.ai/details/publication/pub.1083073897 schema:CreativeWork
274 https://app.dimensions.ai/details/publication/pub.1083118290 schema:CreativeWork
275 https://app.dimensions.ai/details/publication/pub.1083152189 schema:CreativeWork
276 https://app.dimensions.ai/details/publication/pub.1083191040 schema:CreativeWork
277 https://app.dimensions.ai/details/publication/pub.1083197280 schema:CreativeWork
278 https://app.dimensions.ai/details/publication/pub.1083207387 schema:CreativeWork
279 https://app.dimensions.ai/details/publication/pub.1083274021 schema:CreativeWork
280 https://app.dimensions.ai/details/publication/pub.1083288555 schema:CreativeWork
281 https://app.dimensions.ai/details/publication/pub.1083313262 schema:CreativeWork
282 https://app.dimensions.ai/details/publication/pub.1083314035 schema:CreativeWork
283 https://app.dimensions.ai/details/publication/pub.1083335824 schema:CreativeWork
284 https://doi.org/10.1002/jcp.1040900113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050872340
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1002/stem.5530130610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025735562
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1016/s0301-472x(00)00154-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031333805
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1016/s0301-472x(00)00177-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038664403
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1016/s0301-472x(00)00515-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042892402
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1016/s0301-472x(99)00012-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019668782
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1016/s0301-472x(99)00037-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039068293
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1046/j.1365-2141.1999.01677.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015105993
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1073/pnas.93.4.1470 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029011698
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1073/pnas.96.14.8126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040869632
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1073/pnas.97.26.14626 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002642815
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1084/jem.145.6.1612 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004973707
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1084/jem.186.4.619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010498871
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1126/science.283.5403.845 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062564103
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1126/science.8342023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062654735
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1172/jci8583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042806918
315 rdf:type schema:CreativeWork
316 https://www.grid.ac/institutes/grid.6906.9 schema:alternateName Erasmus University Rotterdam
317 schema:name Institute of Hematology, Erasmus University Rotterdam, The Netherlands
318 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...