Identification of false-positive CBFβ/MYH11 RT-PCR results View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-10

AUTHORS

SM Hackwell, Robinson, JF Harvey, FM Ross

ABSTRACT

Persistent problems with false positive results were encountered when carrying out a published RT-PCR method to detect the CBFbeta/MYH11 transcripts associated with the inv(16)(p13q22) cytogenetic abnormality in acute myeloid leukaemia. These were shown to be due to amplification of part of the intronic MYH11 sequence, presumably from very small amounts of contaminating DNA or unspliced primary RNA transcripts, amplified because of partial homology of the CBFbeta3 primer to intronic MYH11 sequence. More... »

PAGES

1617

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.leu.2401545

DOI

http://dx.doi.org/10.1038/sj.leu.2401545

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036067337

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10516764


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acute Disease", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Case-Control Studies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Inversion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytogenetic Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukemia, Myeloid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oncogene Proteins, Fusion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymorphism, Single-Stranded Conformational", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Predictive Value of Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Remission Induction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Salisbury District Hospital", 
          "id": "https://www.grid.ac/institutes/grid.416642.3", 
          "name": [
            "Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hackwell", 
        "givenName": "SM", 
        "id": "sg:person.01123755724.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123755724.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Salisbury District Hospital", 
          "id": "https://www.grid.ac/institutes/grid.416642.3", 
          "name": [
            "Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Robinson", 
        "id": "sg:person.01106426453.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106426453.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Salisbury District Hospital", 
          "id": "https://www.grid.ac/institutes/grid.416642.3", 
          "name": [
            "Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Harvey", 
        "givenName": "JF", 
        "id": "sg:person.0676535134.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676535134.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Salisbury District Hospital", 
          "id": "https://www.grid.ac/institutes/grid.416642.3", 
          "name": [
            "Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ross", 
        "givenName": "FM", 
        "id": "sg:person.0640514314.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640514314.61"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1046/j.1365-2141.1997.d01-2096.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033185407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2141.1997.d01-2096.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033185407"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-10", 
    "datePublishedReg": "1999-10-01", 
    "description": "Persistent problems with false positive results were encountered when carrying out a published RT-PCR method to detect the CBFbeta/MYH11 transcripts associated with the inv(16)(p13q22) cytogenetic abnormality in acute myeloid leukaemia. These were shown to be due to amplification of part of the intronic MYH11 sequence, presumably from very small amounts of contaminating DNA or unspliced primary RNA transcripts, amplified because of partial homology of the CBFbeta3 primer to intronic MYH11 sequence.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.leu.2401545", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1097065", 
        "issn": [
          "0887-6924", 
          "1476-5551"
        ], 
        "name": "Leukemia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "name": "Identification of false-positive CBF\u03b2/MYH11 RT-PCR results", 
    "pagination": "1617", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "abcf95e940b3dfa1791f8c65526dd28d2ecfde485ba9b72d9edd7a43b42624cd"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10516764"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8704895"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.leu.2401545"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036067337"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.leu.2401545", 
      "https://app.dimensions.ai/details/publication/pub.1036067337"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29207_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/2401545"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2401545'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2401545'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2401545'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.leu.2401545'


 

This table displays all metadata directly associated to this object as RDF triples.

136 TRIPLES      21 PREDICATES      41 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.leu.2401545 schema:about N07185c3abb134a0db20091d66ce02afc
2 N4b5b4ed6640347579b3427a976d87584
3 N90a2ca32216440a3a33f8d6b3add8718
4 N9aec9cb30c9e477ea92c82e2ce75c537
5 Na3e1a61f2c9a4995b1f5322b2fea2af7
6 Na9bcb01b39db47318d69dbc5f29a66e4
7 Nac529cd0c3c147d8a17f19bbb164df3b
8 Nd3d9b08399314bfabb4cbec09172eee5
9 Ndea193d8b0bc4e0d8eb5b1bf5da3fdcd
10 Nf7ea82f6c10f47ce8348a2c5766adede
11 Nfafc3dca53b4442c972fa15d2f984f7b
12 anzsrc-for:11
13 anzsrc-for:1112
14 schema:author N6ec18b7188834260ac85bc07b9d28b83
15 schema:citation https://doi.org/10.1046/j.1365-2141.1997.d01-2096.x
16 schema:datePublished 1999-10
17 schema:datePublishedReg 1999-10-01
18 schema:description Persistent problems with false positive results were encountered when carrying out a published RT-PCR method to detect the CBFbeta/MYH11 transcripts associated with the inv(16)(p13q22) cytogenetic abnormality in acute myeloid leukaemia. These were shown to be due to amplification of part of the intronic MYH11 sequence, presumably from very small amounts of contaminating DNA or unspliced primary RNA transcripts, amplified because of partial homology of the CBFbeta3 primer to intronic MYH11 sequence.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree true
22 schema:isPartOf N799685a32fa1427b847e2879d4c1f9a1
23 Nb0574ed9de3647a6aff67dee02324a8d
24 sg:journal.1097065
25 schema:name Identification of false-positive CBFβ/MYH11 RT-PCR results
26 schema:pagination 1617
27 schema:productId N0f882d3fd6d9434e9c82317ccbcc3568
28 N3d31783352c74f508ab8388bce878e83
29 Na70f031b8e4b4d06b8e667108aa10d16
30 Nb85411827c35463fa8f8075470063c76
31 Nbd9a208d0e574e01921a149fc40ca16c
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036067337
33 https://doi.org/10.1038/sj.leu.2401545
34 schema:sdDatePublished 2019-04-11T11:55
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher N7e9cb65e620b469d92ee6794cbfbd860
37 schema:url https://www.nature.com/articles/2401545
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N07185c3abb134a0db20091d66ce02afc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
42 schema:name Case-Control Studies
43 rdf:type schema:DefinedTerm
44 N0f882d3fd6d9434e9c82317ccbcc3568 schema:name dimensions_id
45 schema:value pub.1036067337
46 rdf:type schema:PropertyValue
47 N3d31783352c74f508ab8388bce878e83 schema:name readcube_id
48 schema:value abcf95e940b3dfa1791f8c65526dd28d2ecfde485ba9b72d9edd7a43b42624cd
49 rdf:type schema:PropertyValue
50 N4b5b4ed6640347579b3427a976d87584 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Predictive Value of Tests
52 rdf:type schema:DefinedTerm
53 N6be0bb594cd649f08335407052e0a5ee rdf:first sg:person.01106426453.42
54 rdf:rest Nf3c8c5d526eb4e748eaf995f4dd472c7
55 N6ec18b7188834260ac85bc07b9d28b83 rdf:first sg:person.01123755724.03
56 rdf:rest N6be0bb594cd649f08335407052e0a5ee
57 N6ff6fdf7cf85420f9507afc291be52b0 rdf:first sg:person.0640514314.61
58 rdf:rest rdf:nil
59 N799685a32fa1427b847e2879d4c1f9a1 schema:volumeNumber 13
60 rdf:type schema:PublicationVolume
61 N7e9cb65e620b469d92ee6794cbfbd860 schema:name Springer Nature - SN SciGraph project
62 rdf:type schema:Organization
63 N90a2ca32216440a3a33f8d6b3add8718 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Oncogene Proteins, Fusion
65 rdf:type schema:DefinedTerm
66 N9aec9cb30c9e477ea92c82e2ce75c537 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name Chromosome Inversion
68 rdf:type schema:DefinedTerm
69 Na3e1a61f2c9a4995b1f5322b2fea2af7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Polymorphism, Single-Stranded Conformational
71 rdf:type schema:DefinedTerm
72 Na70f031b8e4b4d06b8e667108aa10d16 schema:name doi
73 schema:value 10.1038/sj.leu.2401545
74 rdf:type schema:PropertyValue
75 Na9bcb01b39db47318d69dbc5f29a66e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Humans
77 rdf:type schema:DefinedTerm
78 Nac529cd0c3c147d8a17f19bbb164df3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Reverse Transcriptase Polymerase Chain Reaction
80 rdf:type schema:DefinedTerm
81 Nb0574ed9de3647a6aff67dee02324a8d schema:issueNumber 10
82 rdf:type schema:PublicationIssue
83 Nb85411827c35463fa8f8075470063c76 schema:name pubmed_id
84 schema:value 10516764
85 rdf:type schema:PropertyValue
86 Nbd9a208d0e574e01921a149fc40ca16c schema:name nlm_unique_id
87 schema:value 8704895
88 rdf:type schema:PropertyValue
89 Nd3d9b08399314bfabb4cbec09172eee5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Leukemia, Myeloid
91 rdf:type schema:DefinedTerm
92 Ndea193d8b0bc4e0d8eb5b1bf5da3fdcd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Acute Disease
94 rdf:type schema:DefinedTerm
95 Nf3c8c5d526eb4e748eaf995f4dd472c7 rdf:first sg:person.0676535134.93
96 rdf:rest N6ff6fdf7cf85420f9507afc291be52b0
97 Nf7ea82f6c10f47ce8348a2c5766adede schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Remission Induction
99 rdf:type schema:DefinedTerm
100 Nfafc3dca53b4442c972fa15d2f984f7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Cytogenetic Analysis
102 rdf:type schema:DefinedTerm
103 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
104 schema:name Medical and Health Sciences
105 rdf:type schema:DefinedTerm
106 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
107 schema:name Oncology and Carcinogenesis
108 rdf:type schema:DefinedTerm
109 sg:journal.1097065 schema:issn 0887-6924
110 1476-5551
111 schema:name Leukemia
112 rdf:type schema:Periodical
113 sg:person.01106426453.42 schema:affiliation https://www.grid.ac/institutes/grid.416642.3
114 schema:familyName Robinson
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106426453.42
116 rdf:type schema:Person
117 sg:person.01123755724.03 schema:affiliation https://www.grid.ac/institutes/grid.416642.3
118 schema:familyName Hackwell
119 schema:givenName SM
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123755724.03
121 rdf:type schema:Person
122 sg:person.0640514314.61 schema:affiliation https://www.grid.ac/institutes/grid.416642.3
123 schema:familyName Ross
124 schema:givenName FM
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640514314.61
126 rdf:type schema:Person
127 sg:person.0676535134.93 schema:affiliation https://www.grid.ac/institutes/grid.416642.3
128 schema:familyName Harvey
129 schema:givenName JF
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676535134.93
131 rdf:type schema:Person
132 https://doi.org/10.1046/j.1365-2141.1997.d01-2096.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1033185407
133 rdf:type schema:CreativeWork
134 https://www.grid.ac/institutes/grid.416642.3 schema:alternateName Salisbury District Hospital
135 schema:name Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK
136 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...