Identification of false-positive CBFβ/MYH11 RT-PCR results View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-10

AUTHORS

SM Hackwell, Robinson, JF Harvey, FM Ross

ABSTRACT

Persistent problems with false positive results were encountered when carrying out a published RT-PCR method to detect the CBFbeta/MYH11 transcripts associated with the inv(16)(p13q22) cytogenetic abnormality in acute myeloid leukaemia. These were shown to be due to amplification of part of the intronic MYH11 sequence, presumably from very small amounts of contaminating DNA or unspliced primary RNA transcripts, amplified because of partial homology of the CBFbeta3 primer to intronic MYH11 sequence. More... »

PAGES

1617

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.leu.2401545

DOI

http://dx.doi.org/10.1038/sj.leu.2401545

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036067337

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10516764


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