Analysis of the INK4a/ARF locus in non-Hodgkin’s lymphomas using two new internal microsatellite markers View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-05

AUTHORS

M Herranz, M Urioste, J Santos, C Rivas, B Martínez, J Benitez, J Fernández-Piqueras

ABSTRACT

Inactivation of the INK4a/ARF locus is a frequent event in non-Hodgkin's lymphomas (NHLs), which may be attributed to deletion, point mutation, and 5' CpG methylation at its promoter region. In the present study we evaluated the occurrence of deletions and genetic instability of INK4a/ARF locus in 30 paired normal and tumor samples of B cell NHLs by conducting an allelotypic analysis with two new polymorphic markers, one located at the intron 1 of p16INK4a gene and the other one placed downstream exon 1beta of p19ARF. Comparison of these results with those obtained in a previous paper using flanking markers (D9S171, D9S942, D9S958 and IFNA) allowed us to detect two new cases of microsatellite instability (L-446 and L-442), and to confirm the occurrence of LOH at the INK4a/ARF locus in one tumor (M-3770). On the contrary, this locus is not affected in three different tumors (L-421, L-272 and L-159) which exhibited LOH at some of the flanking markers. More... »

PAGES

808

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.leu.2401409

DOI

http://dx.doi.org/10.1038/sj.leu.2401409

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036372013

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10374887


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