Binary and mixed population biofilms: Time-lapse image analysis and disinfection with biocides View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-12

AUTHORS

K T Elvers, K Leeming, H M Lappin-Scott

ABSTRACT

Simultaneous binary population biofilm formation by a bacterium and filamentous fungus was demonstrated by time-lapse image analysis in a flow cell system. The accumulation of attached bacterial cells followed an S-shaped graph similar to batch culture bacterial growth, with continual attachment, detachment, rotation, and movement of bacteria over the surface. An extensive hyphal network formed on the surface of the flow cell, protruding into the bulk flow, which subsequently detached. Multiple species mixed fungal–bacterial model biofilms were tested for isothiazolone biocide susceptibility. Biofilms were less susceptible to biocide treatment than planktonic cells of the same organisms. Mixed species biofilms, particularly for the bacterial species, offered greater protection against the action of the biocide compared to single species biofilms. Microbial loss as a result of biocide activity was shown by reduced cell surface coverage in electron micrographs. More... »

PAGES

331-338

References to SciGraph publications

  • 1992. Biofouling in Heat Exchangers in BIOFILMS — SCIENCE AND TECHNOLOGY
  • 2001-03. Binary culture biofilm formation by Stenotrophomonas maltophilia and Fusarium oxysporum in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.jim.7000318

    DOI

    http://dx.doi.org/10.1038/sj.jim.7000318

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010801878

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12483475


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