Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-10

AUTHORS

B S Dien, N N Nichols, R J Bothast

ABSTRACT

Recombinant Escherichia coli have been constructed for the conversion of glucose as well as pentose sugars into L-lactic acid. The strains carry the lactate dehydrogenase gene from Streptococcus bovis on a low copy number plasmid for production of L-lactate. Three E. coli strains were transformed with the plasmid for producing L-lactic acid. Strains FBR9 and FBR11 were serially transferred 10 times in anaerobic cultures in sugar-limited medium containing glucose or xylose without selective antibiotic. An average of 96% of both FBR9 and FBR11 cells maintained pVALDH1 in anaerobic cultures. The fermentation performances of FBR9, FBR10, and FBR11 were compared in pH-controlled batch fermentations with medium containing 10% w/v glucose. Fermentation results were superior for FBR11, an E. coli B strain, compared to those observed for FBR9 or FBR10. FBR11 exhausted the glucose within 30 h, and the maximum lactic acid concentration (7.32% w/v) was 93% of the theoretical maximum. The other side-products detected were cell mass and succinic acid (0.5 g/l). Journal of Industrial Microbiology & Biotechnology (2001) 27, 259–264. More... »

PAGES

259-264

References to SciGraph publications

Journal

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.jim.7000195

    DOI

    http://dx.doi.org/10.1038/sj.jim.7000195

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1052618412

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11687940


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