Partial purification, characterization and nitrogen regulation of the lysine ɛ-aminotransferase of Streptomyces clavuligerus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-04

AUTHORS

J Romero, J F Martín, P Liras, A L Demain, N Rius

ABSTRACT

The L-lysine epsilon-aminotransferase (LAT) of Streptomyces clavuligerus was partially purified and characterized. The 51.3-kDa enzyme exhibited optimal activity at pH 7.0-7.5 and 30 degrees C. It catalyzed transfer of the terminal amino group of L-lysine or L-ornithine to alpha-ketoglutarate. Oxalacetate and pyruvate were also used as acceptors of the amino group but with very low efficiency. Increasing ammonium concentrations added to chemically-defined medium MM enhanced the formation of LAT and decreased production of cephalosporins by S. clavuligerus. In cultures grown in the absence of lysine, greater enhancement of LAT formation by ammonium and less repression of cephalosporin biosynthesis were observed. In the chemically-defined GSPG medium, ammonium ions decreased cephalosporin production without showing an effect on LAT formation. More... »

PAGES

241-246

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.jim.2900370

    DOI

    http://dx.doi.org/10.1038/sj.jim.2900370

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1029996159

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9172431


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