Escherichia coli nitroreductase plus CB1954 enhances the effect of radiotherapy in vitro and in vivo View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-03

AUTHORS

C L White, T Menghistu, K R Twigger, P F Searle, S A Bhide, R G Vile, A A Melcher, H S Pandha, K J Harrington

ABSTRACT

Escherichia coli nitroreductase (NTR) converts the prodrug CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide) into a bifunctional alkylating agent that causes DNA crosslinks. In this study, the ability of NTR to enhance the combined effects of CB1954 and radiation has been tested in vitro and in vivo. Stably transduced ovarian cancer cells (SKOV3-NTR) that are sensitive to CB1954 (IC(50)=0.35 muM) demonstrated enhanced cytotoxicity when treated with CB1954 and single-fraction irradiation. The NTR-CB1954 system mediated a bystander effect in combination with radiation on transfer of conditioned medium from SKOV3-NTR, but not SKOV3, cells to SW480 target cells. The ability of CB1954 to enhance radiation-induced cytotoxicity in SKOV3-NTR (but not SKOV3) cells was also demonstrated by fluorescence-activated cell sorting (FACS) with dual staining for propidium iodide/fluorescein diacetate, 4',6-diamidino-2-phenylindole dichloride staining of apoptotic cells and measurement of double-stranded DNA breaks by FACS and confocal microscopy for gammaH2AX foci. Adenoviral delivery of NTR, under constitutive cytomegalovirus or tissue-specific CTP1 promoters, increased the in vitro cytotoxicity of CB1954 plus radiation in MTT and clonogenic assays. Finally, adenoviral delivery of NTR plus CB1954 enhanced the effect of fractionated radiotherapy (12 Gy in four fractions) in SW480 xenograft tumours in nude mice. More... »

PAGES

3303081

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3303081

DOI

http://dx.doi.org/10.1038/sj.gt.3303081

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031781279

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18079753


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aziridines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Combined Modality Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytomegalovirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Fragmentation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Nude", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitroreductases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ovarian Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Radiation-Sensitizing Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Cancer Research", 
          "id": "https://www.grid.ac/institutes/grid.18886.3f", 
          "name": [
            "Targeted Therapy Laboratory, Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "White", 
        "givenName": "C L", 
        "id": "sg:person.012523221567.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012523221567.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Cancer Research", 
          "id": "https://www.grid.ac/institutes/grid.18886.3f", 
          "name": [
            "Targeted Therapy Laboratory, Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Menghistu", 
        "givenName": "T", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Cancer Research", 
          "id": "https://www.grid.ac/institutes/grid.18886.3f", 
          "name": [
            "Targeted Therapy Laboratory, Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Twigger", 
        "givenName": "K R", 
        "id": "sg:person.01260231270.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260231270.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Birmingham", 
          "id": "https://www.grid.ac/institutes/grid.6572.6", 
          "name": [
            "Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Searle", 
        "givenName": "P F", 
        "id": "sg:person.0633051712.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633051712.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Cancer Research", 
          "id": "https://www.grid.ac/institutes/grid.18886.3f", 
          "name": [
            "Targeted Therapy Laboratory, Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhide", 
        "givenName": "S A", 
        "id": "sg:person.0700143313.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700143313.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Mayo Clinic", 
          "id": "https://www.grid.ac/institutes/grid.66875.3a", 
          "name": [
            "Molecular Medicine Program, Mayo Clinic, Rochester, MN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vile", 
        "givenName": "R G", 
        "id": "sg:person.01207711633.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207711633.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "St James's University Hospital", 
          "id": "https://www.grid.ac/institutes/grid.443984.6", 
          "name": [
            "St James's University Hospital, Leeds, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Melcher", 
        "givenName": "A A", 
        "id": "sg:person.01266210711.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266210711.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "St George's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.464688.0", 
          "name": [
            "St George's Hospital, Cranmer Terrace, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pandha", 
        "givenName": "H S", 
        "id": "sg:person.01215757170.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215757170.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Cancer Research", 
          "id": "https://www.grid.ac/institutes/grid.18886.3f", 
          "name": [
            "Targeted Therapy Laboratory, Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Harrington", 
        "givenName": "K J", 
        "id": "sg:person.01167312032.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167312032.35"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1136/bmj.329.7470.839", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000213545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0360-3016(01)01698-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005334243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(91)90190-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006002692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(91)90190-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006002692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mthe.2001.0468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006833235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0360-3016(02)03005-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014953820"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0360-3016(02)03005-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014953820"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0360-3016(01)02754-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016104941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301196", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019345486", 
          "https://doi.org/10.1038/sj.gt.3301196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301196", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019345486", 
          "https://doi.org/10.1038/sj.gt.3301196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019353382", 
          "https://doi.org/10.1038/sj.gt.3301620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019353382", 
          "https://doi.org/10.1038/sj.gt.3301620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cgt.7700185", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020642765", 
          "https://doi.org/10.1038/sj.cgt.7700185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cgt.7700185", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020642765", 
          "https://doi.org/10.1038/sj.cgt.7700185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-05-0393", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023269713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(92)90671-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023810636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(86)90744-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024696616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-06-0769", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027129963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/clon.2001.0004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031325959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2004.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034993582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-8049(01)00360-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036168945"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cgt.7700506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037799653", 
          "https://doi.org/10.1038/sj.cgt.7700506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cgt.7700506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037799653", 
          "https://doi.org/10.1038/sj.cgt.7700506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/09553008714551451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038284461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(69)90267-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040521796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(69)90267-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040521796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00005392-200012000-00086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060220108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00005392-200012000-00086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060220108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00005392-200012000-00086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060220108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00005392-200012000-00086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060220108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074739240", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074750415", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074880604", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075078689", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075116281", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-03", 
    "datePublishedReg": "2008-03-01", 
    "description": "Escherichia coli nitroreductase (NTR) converts the prodrug CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide) into a bifunctional alkylating agent that causes DNA crosslinks. In this study, the ability of NTR to enhance the combined effects of CB1954 and radiation has been tested in vitro and in vivo. Stably transduced ovarian cancer cells (SKOV3-NTR) that are sensitive to CB1954 (IC(50)=0.35 muM) demonstrated enhanced cytotoxicity when treated with CB1954 and single-fraction irradiation. The NTR-CB1954 system mediated a bystander effect in combination with radiation on transfer of conditioned medium from SKOV3-NTR, but not SKOV3, cells to SW480 target cells. The ability of CB1954 to enhance radiation-induced cytotoxicity in SKOV3-NTR (but not SKOV3) cells was also demonstrated by fluorescence-activated cell sorting (FACS) with dual staining for propidium iodide/fluorescein diacetate, 4',6-diamidino-2-phenylindole dichloride staining of apoptotic cells and measurement of double-stranded DNA breaks by FACS and confocal microscopy for gammaH2AX foci. Adenoviral delivery of NTR, under constitutive cytomegalovirus or tissue-specific CTP1 promoters, increased the in vitro cytotoxicity of CB1954 plus radiation in MTT and clonogenic assays. Finally, adenoviral delivery of NTR plus CB1954 enhanced the effect of fractionated radiotherapy (12 Gy in four fractions) in SW480 xenograft tumours in nude mice.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.gt.3303081", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Escherichia coli nitroreductase plus CB1954 enhances the effect of radiotherapy in vitro and in vivo", 
    "pagination": "3303081", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "976649c7acb0d3ab8a4caf97d023bb2650eb3f9832cc8b35d068f9b45b8bd829"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18079753"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3303081"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1031781279"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3303081", 
      "https://app.dimensions.ai/details/publication/pub.1031781279"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000425.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/3303081"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303081'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303081'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303081'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303081'


 

This table displays all metadata directly associated to this object as RDF triples.

286 TRIPLES      21 PREDICATES      73 URIs      40 LITERALS      28 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3303081 schema:about N0961eb27af93428a9ee247f627c69ae6
2 N09c0d338aada4975bb01006970709f48
3 N0a11204614634f7687d1732d62a9d27d
4 N14ef29bfb047463aa546787fc0bf9609
5 N19e10918e9414a568b20cfb5520f075e
6 N4ada7bf86cfa49e19394d1f0900f1848
7 N4c4ba300e8fb490a968855d4c93292aa
8 N5c7cc8686b784e2ba31a195b1be0c031
9 N673335858a8c400693a3343747d17793
10 N677b893069f246fab32e51112a4e8875
11 N83c08c194db240f9afb97db711e4ca8a
12 N9bd74770d6714845a99dd91b69af8014
13 N9bdb276d094648e7878de044b3a1c8d9
14 Naa3acde9d27c430f86b1df8d81e62b0c
15 Naf66c999d7f34f0bb8c202fab7711473
16 Nbb44f801b0ad4f16929f848c86a9f5a2
17 Ne5ab0f86d2f94d828fbe29953502d5d2
18 Ne788e6c3be4f4316834866129649a3fd
19 Ne793478e630f4f91a63d631b8869d17a
20 anzsrc-for:11
21 anzsrc-for:1112
22 schema:author N0625a8597daf4135a121ca9784a4e449
23 schema:citation sg:pub.10.1038/sj.cgt.7700185
24 sg:pub.10.1038/sj.cgt.7700506
25 sg:pub.10.1038/sj.gt.3301196
26 sg:pub.10.1038/sj.gt.3301620
27 https://app.dimensions.ai/details/publication/pub.1074739240
28 https://app.dimensions.ai/details/publication/pub.1074750415
29 https://app.dimensions.ai/details/publication/pub.1074880604
30 https://app.dimensions.ai/details/publication/pub.1075078689
31 https://app.dimensions.ai/details/publication/pub.1075116281
32 https://doi.org/10.1006/mthe.2001.0468
33 https://doi.org/10.1016/0006-291x(86)90744-8
34 https://doi.org/10.1016/0006-2952(69)90267-6
35 https://doi.org/10.1016/0006-2952(91)90190-g
36 https://doi.org/10.1016/0006-2952(92)90671-5
37 https://doi.org/10.1016/s0360-3016(01)01698-4
38 https://doi.org/10.1016/s0360-3016(01)02754-7
39 https://doi.org/10.1016/s0360-3016(02)03005-5
40 https://doi.org/10.1016/s0959-8049(01)00360-4
41 https://doi.org/10.1053/clon.2001.0004
42 https://doi.org/10.1080/09553008714551451
43 https://doi.org/10.1097/00005392-200012000-00086
44 https://doi.org/10.1136/bmj.329.7470.839
45 https://doi.org/10.1158/0008-5472.can-05-0393
46 https://doi.org/10.1158/1078-0432.ccr-06-0769
47 https://doi.org/10.1200/jco.2004.10.005
48 schema:datePublished 2008-03
49 schema:datePublishedReg 2008-03-01
50 schema:description Escherichia coli nitroreductase (NTR) converts the prodrug CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide) into a bifunctional alkylating agent that causes DNA crosslinks. In this study, the ability of NTR to enhance the combined effects of CB1954 and radiation has been tested in vitro and in vivo. Stably transduced ovarian cancer cells (SKOV3-NTR) that are sensitive to CB1954 (IC(50)=0.35 muM) demonstrated enhanced cytotoxicity when treated with CB1954 and single-fraction irradiation. The NTR-CB1954 system mediated a bystander effect in combination with radiation on transfer of conditioned medium from SKOV3-NTR, but not SKOV3, cells to SW480 target cells. The ability of CB1954 to enhance radiation-induced cytotoxicity in SKOV3-NTR (but not SKOV3) cells was also demonstrated by fluorescence-activated cell sorting (FACS) with dual staining for propidium iodide/fluorescein diacetate, 4',6-diamidino-2-phenylindole dichloride staining of apoptotic cells and measurement of double-stranded DNA breaks by FACS and confocal microscopy for gammaH2AX foci. Adenoviral delivery of NTR, under constitutive cytomegalovirus or tissue-specific CTP1 promoters, increased the in vitro cytotoxicity of CB1954 plus radiation in MTT and clonogenic assays. Finally, adenoviral delivery of NTR plus CB1954 enhanced the effect of fractionated radiotherapy (12 Gy in four fractions) in SW480 xenograft tumours in nude mice.
51 schema:genre research_article
52 schema:inLanguage en
53 schema:isAccessibleForFree true
54 schema:isPartOf Nd52541d5f98046a8afcb0be3370bd371
55 Ne66d176a25c94272b1bd7498e114f741
56 sg:journal.1105638
57 schema:name Escherichia coli nitroreductase plus CB1954 enhances the effect of radiotherapy in vitro and in vivo
58 schema:pagination 3303081
59 schema:productId N3ae344036272470c86d1c15cce661c40
60 N516ba449624647099066d73f5b6766a2
61 Nad782a8316184ecf86880d4d5b6fd970
62 Nbee75b875e3c488085a1ba17ec1e040e
63 Nd25985e6f83b4abb9ab8452303530426
64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031781279
65 https://doi.org/10.1038/sj.gt.3303081
66 schema:sdDatePublished 2019-04-10T13:55
67 schema:sdLicense https://scigraph.springernature.com/explorer/license/
68 schema:sdPublisher Nde49a215d6384da882b928a835c3080b
69 schema:url http://www.nature.com/articles/3303081
70 sgo:license sg:explorer/license/
71 sgo:sdDataset articles
72 rdf:type schema:ScholarlyArticle
73 N0625a8597daf4135a121ca9784a4e449 rdf:first sg:person.012523221567.24
74 rdf:rest N07625d776bbf41d6b7518143742794df
75 N07625d776bbf41d6b7518143742794df rdf:first N1509a29ae03e4445a48815d7630a7cb8
76 rdf:rest Ne7709a59f0964ff4be5e7dcc989f0573
77 N0961eb27af93428a9ee247f627c69ae6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Mice, Nude
79 rdf:type schema:DefinedTerm
80 N09c0d338aada4975bb01006970709f48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Escherichia coli
82 rdf:type schema:DefinedTerm
83 N0a11204614634f7687d1732d62a9d27d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name DNA Fragmentation
85 rdf:type schema:DefinedTerm
86 N14ef29bfb047463aa546787fc0bf9609 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Radiation-Sensitizing Agents
88 rdf:type schema:DefinedTerm
89 N1509a29ae03e4445a48815d7630a7cb8 schema:affiliation https://www.grid.ac/institutes/grid.18886.3f
90 schema:familyName Menghistu
91 schema:givenName T
92 rdf:type schema:Person
93 N19e10918e9414a568b20cfb5520f075e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Animals
95 rdf:type schema:DefinedTerm
96 N2ea9f30553434ee4b62f810a30c36a65 rdf:first sg:person.01215757170.08
97 rdf:rest Nd1ec618f18fb48968ebddb2a3b9e7192
98 N3ae344036272470c86d1c15cce661c40 schema:name dimensions_id
99 schema:value pub.1031781279
100 rdf:type schema:PropertyValue
101 N4ada7bf86cfa49e19394d1f0900f1848 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Mice
103 rdf:type schema:DefinedTerm
104 N4c4ba300e8fb490a968855d4c93292aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Transduction, Genetic
106 rdf:type schema:DefinedTerm
107 N516ba449624647099066d73f5b6766a2 schema:name doi
108 schema:value 10.1038/sj.gt.3303081
109 rdf:type schema:PropertyValue
110 N5801576d0eb54240a5e0dc9d3848fd6e rdf:first sg:person.01207711633.71
111 rdf:rest N6741c35ba43e491f9a57263ec930fb6e
112 N5c7cc8686b784e2ba31a195b1be0c031 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Nitroreductases
114 rdf:type schema:DefinedTerm
115 N673335858a8c400693a3343747d17793 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Flow Cytometry
117 rdf:type schema:DefinedTerm
118 N6741c35ba43e491f9a57263ec930fb6e rdf:first sg:person.01266210711.38
119 rdf:rest N2ea9f30553434ee4b62f810a30c36a65
120 N677b893069f246fab32e51112a4e8875 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Genetic Vectors
122 rdf:type schema:DefinedTerm
123 N83c08c194db240f9afb97db711e4ca8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Combined Modality Therapy
125 rdf:type schema:DefinedTerm
126 N9bae8ca3a4804f7e938aa1c94dc15573 rdf:first sg:person.0700143313.76
127 rdf:rest N5801576d0eb54240a5e0dc9d3848fd6e
128 N9bd74770d6714845a99dd91b69af8014 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Genetic Therapy
130 rdf:type schema:DefinedTerm
131 N9bdb276d094648e7878de044b3a1c8d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Ovarian Neoplasms
133 rdf:type schema:DefinedTerm
134 Naa3acde9d27c430f86b1df8d81e62b0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Cytomegalovirus
136 rdf:type schema:DefinedTerm
137 Nad782a8316184ecf86880d4d5b6fd970 schema:name nlm_unique_id
138 schema:value 9421525
139 rdf:type schema:PropertyValue
140 Naf66c999d7f34f0bb8c202fab7711473 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Cell Line, Tumor
142 rdf:type schema:DefinedTerm
143 Nbb44f801b0ad4f16929f848c86a9f5a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Apoptosis
145 rdf:type schema:DefinedTerm
146 Nbee75b875e3c488085a1ba17ec1e040e schema:name readcube_id
147 schema:value 976649c7acb0d3ab8a4caf97d023bb2650eb3f9832cc8b35d068f9b45b8bd829
148 rdf:type schema:PropertyValue
149 Nd1ec618f18fb48968ebddb2a3b9e7192 rdf:first sg:person.01167312032.35
150 rdf:rest rdf:nil
151 Nd25985e6f83b4abb9ab8452303530426 schema:name pubmed_id
152 schema:value 18079753
153 rdf:type schema:PropertyValue
154 Nd52541d5f98046a8afcb0be3370bd371 schema:volumeNumber 15
155 rdf:type schema:PublicationVolume
156 Nde49a215d6384da882b928a835c3080b schema:name Springer Nature - SN SciGraph project
157 rdf:type schema:Organization
158 Ne5ab0f86d2f94d828fbe29953502d5d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Neoplasm Transplantation
160 rdf:type schema:DefinedTerm
161 Ne66d176a25c94272b1bd7498e114f741 schema:issueNumber 6
162 rdf:type schema:PublicationIssue
163 Ne7709a59f0964ff4be5e7dcc989f0573 rdf:first sg:person.01260231270.15
164 rdf:rest Nf0d4f5cbd2a1453ea2cfcaddb1e59bb2
165 Ne788e6c3be4f4316834866129649a3fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Female
167 rdf:type schema:DefinedTerm
168 Ne793478e630f4f91a63d631b8869d17a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Aziridines
170 rdf:type schema:DefinedTerm
171 Nf0d4f5cbd2a1453ea2cfcaddb1e59bb2 rdf:first sg:person.0633051712.02
172 rdf:rest N9bae8ca3a4804f7e938aa1c94dc15573
173 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
174 schema:name Medical and Health Sciences
175 rdf:type schema:DefinedTerm
176 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
177 schema:name Oncology and Carcinogenesis
178 rdf:type schema:DefinedTerm
179 sg:journal.1105638 schema:issn 0969-7128
180 1476-5462
181 schema:name Gene Therapy
182 rdf:type schema:Periodical
183 sg:person.01167312032.35 schema:affiliation https://www.grid.ac/institutes/grid.18886.3f
184 schema:familyName Harrington
185 schema:givenName K J
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167312032.35
187 rdf:type schema:Person
188 sg:person.01207711633.71 schema:affiliation https://www.grid.ac/institutes/grid.66875.3a
189 schema:familyName Vile
190 schema:givenName R G
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207711633.71
192 rdf:type schema:Person
193 sg:person.01215757170.08 schema:affiliation https://www.grid.ac/institutes/grid.464688.0
194 schema:familyName Pandha
195 schema:givenName H S
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215757170.08
197 rdf:type schema:Person
198 sg:person.012523221567.24 schema:affiliation https://www.grid.ac/institutes/grid.18886.3f
199 schema:familyName White
200 schema:givenName C L
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012523221567.24
202 rdf:type schema:Person
203 sg:person.01260231270.15 schema:affiliation https://www.grid.ac/institutes/grid.18886.3f
204 schema:familyName Twigger
205 schema:givenName K R
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260231270.15
207 rdf:type schema:Person
208 sg:person.01266210711.38 schema:affiliation https://www.grid.ac/institutes/grid.443984.6
209 schema:familyName Melcher
210 schema:givenName A A
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266210711.38
212 rdf:type schema:Person
213 sg:person.0633051712.02 schema:affiliation https://www.grid.ac/institutes/grid.6572.6
214 schema:familyName Searle
215 schema:givenName P F
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633051712.02
217 rdf:type schema:Person
218 sg:person.0700143313.76 schema:affiliation https://www.grid.ac/institutes/grid.18886.3f
219 schema:familyName Bhide
220 schema:givenName S A
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700143313.76
222 rdf:type schema:Person
223 sg:pub.10.1038/sj.cgt.7700185 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020642765
224 https://doi.org/10.1038/sj.cgt.7700185
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/sj.cgt.7700506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037799653
227 https://doi.org/10.1038/sj.cgt.7700506
228 rdf:type schema:CreativeWork
229 sg:pub.10.1038/sj.gt.3301196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019345486
230 https://doi.org/10.1038/sj.gt.3301196
231 rdf:type schema:CreativeWork
232 sg:pub.10.1038/sj.gt.3301620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019353382
233 https://doi.org/10.1038/sj.gt.3301620
234 rdf:type schema:CreativeWork
235 https://app.dimensions.ai/details/publication/pub.1074739240 schema:CreativeWork
236 https://app.dimensions.ai/details/publication/pub.1074750415 schema:CreativeWork
237 https://app.dimensions.ai/details/publication/pub.1074880604 schema:CreativeWork
238 https://app.dimensions.ai/details/publication/pub.1075078689 schema:CreativeWork
239 https://app.dimensions.ai/details/publication/pub.1075116281 schema:CreativeWork
240 https://doi.org/10.1006/mthe.2001.0468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006833235
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/0006-291x(86)90744-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024696616
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/0006-2952(69)90267-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040521796
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/0006-2952(91)90190-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1006002692
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/0006-2952(92)90671-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023810636
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/s0360-3016(01)01698-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005334243
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s0360-3016(01)02754-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016104941
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s0360-3016(02)03005-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014953820
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/s0959-8049(01)00360-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036168945
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1053/clon.2001.0004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031325959
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1080/09553008714551451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038284461
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1097/00005392-200012000-00086 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060220108
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1136/bmj.329.7470.839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000213545
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1158/0008-5472.can-05-0393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023269713
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1158/1078-0432.ccr-06-0769 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027129963
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1200/jco.2004.10.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034993582
271 rdf:type schema:CreativeWork
272 https://www.grid.ac/institutes/grid.18886.3f schema:alternateName Institute of Cancer Research
273 schema:name Targeted Therapy Laboratory, Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, UK
274 rdf:type schema:Organization
275 https://www.grid.ac/institutes/grid.443984.6 schema:alternateName St James's University Hospital
276 schema:name St James's University Hospital, Leeds, UK
277 rdf:type schema:Organization
278 https://www.grid.ac/institutes/grid.464688.0 schema:alternateName St George's Hospital
279 schema:name St George's Hospital, Cranmer Terrace, London, UK
280 rdf:type schema:Organization
281 https://www.grid.ac/institutes/grid.6572.6 schema:alternateName University of Birmingham
282 schema:name Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, UK
283 rdf:type schema:Organization
284 https://www.grid.ac/institutes/grid.66875.3a schema:alternateName Mayo Clinic
285 schema:name Molecular Medicine Program, Mayo Clinic, Rochester, MN, USA
286 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...