A lentiviral vector with a short troponin-I promoter for tracking cardiomyocyte differentiation of human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-02

AUTHORS

P Gallo, S Grimaldi, M V G Latronico, D Bonci, A Pagliuca, P Gallo, S Ausoni, C Peschle, G Condorelli

ABSTRACT

Human embryonic stem cells (hESCs) may become important for cardiac repair due to their potentially unlimited ability to generate cardiomyocytes (CMCs). Moreover, genetic manipulation of hESC-derived CMCs would be a very promising technique for curing myocardial disorders. At the present time, however, inducing the differentiation of hESCs into CMCs is extremely difficult and, therefore, an easy and standardizable technique is needed to evaluate differentiation strategies. Vectors driving cardiac-specific expression may represent an important tool not only for monitoring new cardiac-differentiation strategies, but also for the manipulation of cardiac differentiation of ESCs. To this aim, we generated cardiac-specific lentiviral vectors (LVVs) in which expression is driven by a short fragment of the cardiac troponin-I proximal promoter (TNNI3) with a human cardiac alpha-actin enhancer, and tested its suitability in inducing tissue-specific gene expression and ability to track the CMC lineage during differentiation of ESCs. We determined that (1) TNNI3-LVVs efficiently drive cardiac-specific gene expression and mark the cardiomyogenic lineage in human and mouse ESC differentiation systems (2) the cardiac alpha-actin enhancer confers a further increase in gene-expression specificity of TNNI3-LVVs in hESCs. Although this technique may not be useful in tracking small numbers of cells, data suggested that TNNI3-based LVVs are a powerful tool for manipulating human ESCs and modifying hESC-derived CMCs. More... »

PAGES

161

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3303017

DOI

http://dx.doi.org/10.1038/sj.gt.3303017

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010306179

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18033312


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enhancer Elements, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Heart Failure", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lentivirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myocytes, Cardiac", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Troponin I", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Laboratory of Molecular Cardiology, San Raffaele Biomedical Science Park Foundation, Rome, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gallo", 
        "givenName": "P", 
        "type": "Person"
      }, 
      {
        "familyName": "Grimaldi", 
        "givenName": "S", 
        "id": "sg:person.0631453213.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631453213.21"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Latronico", 
        "givenName": "M V G", 
        "id": "sg:person.0576137377.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576137377.72"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Bonci", 
        "givenName": "D", 
        "type": "Person"
      }, 
      {
        "familyName": "Pagliuca", 
        "givenName": "A", 
        "id": "sg:person.01250177667.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250177667.45"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Gallo", 
        "givenName": "P", 
        "type": "Person"
      }, 
      {
        "familyName": "Ausoni", 
        "givenName": "S", 
        "id": "sg:person.01240054113.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240054113.01"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Peschle", 
        "givenName": "C", 
        "id": "sg:person.056721310.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.056721310.67"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Condorelli", 
        "givenName": "G", 
        "id": "sg:person.016367365420.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016367365420.10"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1385/1-59259-241-4:27", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001295163", 
          "https://doi.org/10.1385/1-59259-241-4:27"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncpcardio0738", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005628204", 
          "https://doi.org/10.1038/ncpcardio0738"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci118769", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016900524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1469-7580.2002.00031.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017225669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m411082200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017795281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301936", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018676645", 
          "https://doi.org/10.1038/sj.gt.3301936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301936", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018676645", 
          "https://doi.org/10.1038/sj.gt.3301936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0500169102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021548019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.cir.0000068356.38592.68", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021831087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmsr040330", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022304644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023845087", 
          "https://doi.org/10.1038/nbt1014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023845087", 
          "https://doi.org/10.1038/nbt1014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1991.tb00865.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027679400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.282.5391.1145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028898955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.0000035254.80718.91", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032098280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1303605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033143053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.0000147315.71699.51", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033809756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.0000147315.71699.51", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033809756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00687-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037266204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m310822200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039474006"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1097-0177(2000)9999:9999<::aid-dvdy1049>3.0.co;2-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043587524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(05)67380-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044557286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(05)67380-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044557286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/83324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292282", 
          "https://doi.org/10.1038/83324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/83324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292282", 
          "https://doi.org/10.1038/83324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(01)00144-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047314499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.2132126100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048440616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/geno.1996.0317", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049143710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.21-1-111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051806446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.0000027865.61704.32", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052760675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2004.13.460", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059309410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2005.14.367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059309470"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci12131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063416054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074971079", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079607947", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082427042", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082734746", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083109250", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083326800", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-02", 
    "datePublishedReg": "2008-02-01", 
    "description": "Human embryonic stem cells (hESCs) may become important for cardiac repair due to their potentially unlimited ability to generate cardiomyocytes (CMCs). Moreover, genetic manipulation of hESC-derived CMCs would be a very promising technique for curing myocardial disorders. At the present time, however, inducing the differentiation of hESCs into CMCs is extremely difficult and, therefore, an easy and standardizable technique is needed to evaluate differentiation strategies. Vectors driving cardiac-specific expression may represent an important tool not only for monitoring new cardiac-differentiation strategies, but also for the manipulation of cardiac differentiation of ESCs. To this aim, we generated cardiac-specific lentiviral vectors (LVVs) in which expression is driven by a short fragment of the cardiac troponin-I proximal promoter (TNNI3) with a human cardiac alpha-actin enhancer, and tested its suitability in inducing tissue-specific gene expression and ability to track the CMC lineage during differentiation of ESCs. We determined that (1) TNNI3-LVVs efficiently drive cardiac-specific gene expression and mark the cardiomyogenic lineage in human and mouse ESC differentiation systems (2) the cardiac alpha-actin enhancer confers a further increase in gene-expression specificity of TNNI3-LVVs in hESCs. Although this technique may not be useful in tracking small numbers of cells, data suggested that TNNI3-based LVVs are a powerful tool for manipulating human ESCs and modifying hESC-derived CMCs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.gt.3303017", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "A lentiviral vector with a short troponin-I promoter for tracking cardiomyocyte differentiation of human embryonic stem cells", 
    "pagination": "161", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "31199862634695b09a4f3da7746640a8a08b77e26bcf4ca81366308ae37ca712"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18033312"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3303017"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010306179"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3303017", 
      "https://app.dimensions.ai/details/publication/pub.1010306179"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T22:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000422.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/3303017"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303017'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303017'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303017'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3303017'


 

This table displays all metadata directly associated to this object as RDF triples.

286 TRIPLES      21 PREDICATES      81 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3303017 schema:about N01edb36fd5274fe2af92c4ec15a92f67
2 N07701b560d624298bed75a10bd4b9118
3 N0e2ca32162104e7cb0da41fd9fd37da4
4 N1805a03ff60a4ce283ba52bd5c783d62
5 N22bc7bbf05714c95a7c2d6ecce0960b6
6 N296c6f36ee15451b941bd08b5643ec85
7 N39aeb8541a7a49588b285d23c32a11e3
8 N578a761a40ed4a9b9d14bfc26051fef5
9 N60b1d452bd01403eaf4a1aa23bd24042
10 N6cf7897c908343f4adba003922916c94
11 N7ff6c6d13fdd4e9a8645678b8a0b2a4f
12 Nad4c763233d94ca1bc4c6885ae2e4caf
13 Nbcaf01b788e341a3a0965d6e78642c0c
14 Nc64eb7e023c04bbdb8db442505594c58
15 Ned7de8de350d426cb2a53d58749929a3
16 Nfabd246d9ce746fd87dde31f85fa61d8
17 Nfd8b6d884ac644ef808cbdab6d1403ab
18 Nff73333112a949b3a425d6f4b6172a92
19 anzsrc-for:06
20 anzsrc-for:0604
21 schema:author Ndc8a569b2d3549fcab4836f928e13b27
22 schema:citation sg:pub.10.1038/83324
23 sg:pub.10.1038/nbt1014
24 sg:pub.10.1038/ncpcardio0738
25 sg:pub.10.1038/sj.gt.3301936
26 sg:pub.10.1385/1-59259-241-4:27
27 https://app.dimensions.ai/details/publication/pub.1074971079
28 https://app.dimensions.ai/details/publication/pub.1079607947
29 https://app.dimensions.ai/details/publication/pub.1082427042
30 https://app.dimensions.ai/details/publication/pub.1082734746
31 https://app.dimensions.ai/details/publication/pub.1083109250
32 https://app.dimensions.ai/details/publication/pub.1083326800
33 https://doi.org/10.1002/1097-0177(2000)9999:9999<::aid-dvdy1049>3.0.co;2-0
34 https://doi.org/10.1006/geno.1996.0317
35 https://doi.org/10.1016/s0092-8674(03)00687-1
36 https://doi.org/10.1016/s0140-6736(05)67380-1
37 https://doi.org/10.1016/s0960-9822(01)00144-0
38 https://doi.org/10.1046/j.1469-7580.2002.00031.x
39 https://doi.org/10.1056/nejmsr040330
40 https://doi.org/10.1073/pnas.0500169102
41 https://doi.org/10.1073/pnas.2132126100
42 https://doi.org/10.1074/jbc.m310822200
43 https://doi.org/10.1074/jbc.m411082200
44 https://doi.org/10.1089/scd.2004.13.460
45 https://doi.org/10.1089/scd.2005.14.367
46 https://doi.org/10.1101/gad.1303605
47 https://doi.org/10.1111/j.1432-0436.1991.tb00865.x
48 https://doi.org/10.1126/science.282.5391.1145
49 https://doi.org/10.1161/01.cir.0000068356.38592.68
50 https://doi.org/10.1161/01.res.0000027865.61704.32
51 https://doi.org/10.1161/01.res.0000035254.80718.91
52 https://doi.org/10.1161/01.res.0000147315.71699.51
53 https://doi.org/10.1172/jci118769
54 https://doi.org/10.1172/jci12131
55 https://doi.org/10.1634/stemcells.21-1-111
56 schema:datePublished 2008-02
57 schema:datePublishedReg 2008-02-01
58 schema:description Human embryonic stem cells (hESCs) may become important for cardiac repair due to their potentially unlimited ability to generate cardiomyocytes (CMCs). Moreover, genetic manipulation of hESC-derived CMCs would be a very promising technique for curing myocardial disorders. At the present time, however, inducing the differentiation of hESCs into CMCs is extremely difficult and, therefore, an easy and standardizable technique is needed to evaluate differentiation strategies. Vectors driving cardiac-specific expression may represent an important tool not only for monitoring new cardiac-differentiation strategies, but also for the manipulation of cardiac differentiation of ESCs. To this aim, we generated cardiac-specific lentiviral vectors (LVVs) in which expression is driven by a short fragment of the cardiac troponin-I proximal promoter (TNNI3) with a human cardiac alpha-actin enhancer, and tested its suitability in inducing tissue-specific gene expression and ability to track the CMC lineage during differentiation of ESCs. We determined that (1) TNNI3-LVVs efficiently drive cardiac-specific gene expression and mark the cardiomyogenic lineage in human and mouse ESC differentiation systems (2) the cardiac alpha-actin enhancer confers a further increase in gene-expression specificity of TNNI3-LVVs in hESCs. Although this technique may not be useful in tracking small numbers of cells, data suggested that TNNI3-based LVVs are a powerful tool for manipulating human ESCs and modifying hESC-derived CMCs.
59 schema:genre research_article
60 schema:inLanguage en
61 schema:isAccessibleForFree true
62 schema:isPartOf N6f0249fd54d64c419884c6615daf0974
63 N91468ae4cf354865aff2dd6285a67979
64 sg:journal.1105638
65 schema:name A lentiviral vector with a short troponin-I promoter for tracking cardiomyocyte differentiation of human embryonic stem cells
66 schema:pagination 161
67 schema:productId N6c2d3c04ee6943bb91eb7fcd38933910
68 Na5f65a03c4aa420ebe5f2eb3d47ac877
69 Nedb9bffa09f04a1c90c196c777462749
70 Nedbc6831896946faa616870ecb0a890c
71 Nf74661d48e3949f69f7fe161a18cf884
72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010306179
73 https://doi.org/10.1038/sj.gt.3303017
74 schema:sdDatePublished 2019-04-10T22:19
75 schema:sdLicense https://scigraph.springernature.com/explorer/license/
76 schema:sdPublisher N59c76eb8345244ab95abe69cfb662a63
77 schema:url https://www.nature.com/articles/3303017
78 sgo:license sg:explorer/license/
79 sgo:sdDataset articles
80 rdf:type schema:ScholarlyArticle
81 N01edb36fd5274fe2af92c4ec15a92f67 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Enhancer Elements, Genetic
83 rdf:type schema:DefinedTerm
84 N07701b560d624298bed75a10bd4b9118 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Heart Failure
86 rdf:type schema:DefinedTerm
87 N0e2ca32162104e7cb0da41fd9fd37da4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Genetic Vectors
89 rdf:type schema:DefinedTerm
90 N12726ee8a0e144cdb78c8a0b31645547 rdf:first sg:person.0576137377.72
91 rdf:rest N80d931dcdc8a4cf2869ef52a139c5410
92 N1805a03ff60a4ce283ba52bd5c783d62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Transduction, Genetic
94 rdf:type schema:DefinedTerm
95 N22bc7bbf05714c95a7c2d6ecce0960b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Animals
97 rdf:type schema:DefinedTerm
98 N296c6f36ee15451b941bd08b5643ec85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Troponin I
100 rdf:type schema:DefinedTerm
101 N39aeb8541a7a49588b285d23c32a11e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Humans
103 rdf:type schema:DefinedTerm
104 N54c05ab681ce44ac95ea161c6940e34b schema:familyName Bonci
105 schema:givenName D
106 rdf:type schema:Person
107 N578a761a40ed4a9b9d14bfc26051fef5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Cell Line
109 rdf:type schema:DefinedTerm
110 N59c76eb8345244ab95abe69cfb662a63 schema:name Springer Nature - SN SciGraph project
111 rdf:type schema:Organization
112 N60b1d452bd01403eaf4a1aa23bd24042 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Embryonic Stem Cells
114 rdf:type schema:DefinedTerm
115 N6398f96f5fc7437698ae9fcbc54f0649 rdf:first sg:person.056721310.67
116 rdf:rest N7c575d871bc149c5ae1a7ee8b996733d
117 N6c2d3c04ee6943bb91eb7fcd38933910 schema:name readcube_id
118 schema:value 31199862634695b09a4f3da7746640a8a08b77e26bcf4ca81366308ae37ca712
119 rdf:type schema:PropertyValue
120 N6cf7897c908343f4adba003922916c94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Genetic Therapy
122 rdf:type schema:DefinedTerm
123 N6f0249fd54d64c419884c6615daf0974 schema:issueNumber 3
124 rdf:type schema:PublicationIssue
125 N7c575d871bc149c5ae1a7ee8b996733d rdf:first sg:person.016367365420.10
126 rdf:rest rdf:nil
127 N7ff6c6d13fdd4e9a8645678b8a0b2a4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Genetic Engineering
129 rdf:type schema:DefinedTerm
130 N80d931dcdc8a4cf2869ef52a139c5410 rdf:first N54c05ab681ce44ac95ea161c6940e34b
131 rdf:rest N89e907b4675d48faa27df226b92a3c34
132 N85b10d2a6bfa4a5f854c7e66674a591a schema:name Laboratory of Molecular Cardiology, San Raffaele Biomedical Science Park Foundation, Rome, Italy.
133 rdf:type schema:Organization
134 N89e907b4675d48faa27df226b92a3c34 rdf:first sg:person.01250177667.45
135 rdf:rest Nebf91782b6ad4c4ba228d265f46c5f54
136 N91468ae4cf354865aff2dd6285a67979 schema:volumeNumber 15
137 rdf:type schema:PublicationVolume
138 Na2fee76ec22e4da8bcb834e0cee577af schema:familyName Gallo
139 schema:givenName P
140 rdf:type schema:Person
141 Na5f65a03c4aa420ebe5f2eb3d47ac877 schema:name doi
142 schema:value 10.1038/sj.gt.3303017
143 rdf:type schema:PropertyValue
144 Nad4c763233d94ca1bc4c6885ae2e4caf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Flow Cytometry
146 rdf:type schema:DefinedTerm
147 Nbcaf01b788e341a3a0965d6e78642c0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Myocytes, Cardiac
149 rdf:type schema:DefinedTerm
150 Nc64eb7e023c04bbdb8db442505594c58 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Promoter Regions, Genetic
152 rdf:type schema:DefinedTerm
153 Ncf11049954f34d8eaca44a7e1e60a599 rdf:first sg:person.01240054113.01
154 rdf:rest N6398f96f5fc7437698ae9fcbc54f0649
155 Nd926d37580f24b919dafce520622c91e rdf:first sg:person.0631453213.21
156 rdf:rest N12726ee8a0e144cdb78c8a0b31645547
157 Ndc8a569b2d3549fcab4836f928e13b27 rdf:first Ne66e07b27acf4388ad74dc9e022ce83f
158 rdf:rest Nd926d37580f24b919dafce520622c91e
159 Ne66e07b27acf4388ad74dc9e022ce83f schema:affiliation N85b10d2a6bfa4a5f854c7e66674a591a
160 schema:familyName Gallo
161 schema:givenName P
162 rdf:type schema:Person
163 Nebf91782b6ad4c4ba228d265f46c5f54 rdf:first Na2fee76ec22e4da8bcb834e0cee577af
164 rdf:rest Ncf11049954f34d8eaca44a7e1e60a599
165 Ned7de8de350d426cb2a53d58749929a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Actins
167 rdf:type schema:DefinedTerm
168 Nedb9bffa09f04a1c90c196c777462749 schema:name dimensions_id
169 schema:value pub.1010306179
170 rdf:type schema:PropertyValue
171 Nedbc6831896946faa616870ecb0a890c schema:name pubmed_id
172 schema:value 18033312
173 rdf:type schema:PropertyValue
174 Nf74661d48e3949f69f7fe161a18cf884 schema:name nlm_unique_id
175 schema:value 9421525
176 rdf:type schema:PropertyValue
177 Nfabd246d9ce746fd87dde31f85fa61d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Mice
179 rdf:type schema:DefinedTerm
180 Nfd8b6d884ac644ef808cbdab6d1403ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Lentivirus
182 rdf:type schema:DefinedTerm
183 Nff73333112a949b3a425d6f4b6172a92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Cell Differentiation
185 rdf:type schema:DefinedTerm
186 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
187 schema:name Biological Sciences
188 rdf:type schema:DefinedTerm
189 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
190 schema:name Genetics
191 rdf:type schema:DefinedTerm
192 sg:journal.1105638 schema:issn 0969-7128
193 1476-5462
194 schema:name Gene Therapy
195 rdf:type schema:Periodical
196 sg:person.01240054113.01 schema:familyName Ausoni
197 schema:givenName S
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240054113.01
199 rdf:type schema:Person
200 sg:person.01250177667.45 schema:familyName Pagliuca
201 schema:givenName A
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250177667.45
203 rdf:type schema:Person
204 sg:person.016367365420.10 schema:familyName Condorelli
205 schema:givenName G
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016367365420.10
207 rdf:type schema:Person
208 sg:person.056721310.67 schema:familyName Peschle
209 schema:givenName C
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.056721310.67
211 rdf:type schema:Person
212 sg:person.0576137377.72 schema:familyName Latronico
213 schema:givenName M V G
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576137377.72
215 rdf:type schema:Person
216 sg:person.0631453213.21 schema:familyName Grimaldi
217 schema:givenName S
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631453213.21
219 rdf:type schema:Person
220 sg:pub.10.1038/83324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047292282
221 https://doi.org/10.1038/83324
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/nbt1014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023845087
224 https://doi.org/10.1038/nbt1014
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/ncpcardio0738 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005628204
227 https://doi.org/10.1038/ncpcardio0738
228 rdf:type schema:CreativeWork
229 sg:pub.10.1038/sj.gt.3301936 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018676645
230 https://doi.org/10.1038/sj.gt.3301936
231 rdf:type schema:CreativeWork
232 sg:pub.10.1385/1-59259-241-4:27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001295163
233 https://doi.org/10.1385/1-59259-241-4:27
234 rdf:type schema:CreativeWork
235 https://app.dimensions.ai/details/publication/pub.1074971079 schema:CreativeWork
236 https://app.dimensions.ai/details/publication/pub.1079607947 schema:CreativeWork
237 https://app.dimensions.ai/details/publication/pub.1082427042 schema:CreativeWork
238 https://app.dimensions.ai/details/publication/pub.1082734746 schema:CreativeWork
239 https://app.dimensions.ai/details/publication/pub.1083109250 schema:CreativeWork
240 https://app.dimensions.ai/details/publication/pub.1083326800 schema:CreativeWork
241 https://doi.org/10.1002/1097-0177(2000)9999:9999<::aid-dvdy1049>3.0.co;2-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043587524
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1006/geno.1996.0317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049143710
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/s0092-8674(03)00687-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037266204
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/s0140-6736(05)67380-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044557286
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/s0960-9822(01)00144-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047314499
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1046/j.1469-7580.2002.00031.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017225669
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1056/nejmsr040330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022304644
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1073/pnas.0500169102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021548019
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1073/pnas.2132126100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048440616
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1074/jbc.m310822200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039474006
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1074/jbc.m411082200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017795281
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1089/scd.2004.13.460 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059309410
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1089/scd.2005.14.367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059309470
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1101/gad.1303605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033143053
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1111/j.1432-0436.1991.tb00865.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027679400
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1161/01.cir.0000068356.38592.68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021831087
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1161/01.res.0000027865.61704.32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052760675
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1161/01.res.0000035254.80718.91 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032098280
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1161/01.res.0000147315.71699.51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033809756
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1172/jci118769 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016900524
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1172/jci12131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063416054
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1634/stemcells.21-1-111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051806446
286 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...