Genetic design of an optimized packaging cell line for gene vectors transducing human B cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-05

AUTHORS

E Hettich, A Janz, R Zeidler, D Pich, E Hellebrand, B Weissflog, A Moosmann, W Hammerschmidt

ABSTRACT

Viral gene vectors often rely on packaging cell lines, which provide the necessary factors in trans for the formation of virus-like particles. Previously, we reported on a first-generation packaging cell line for gene vectors, which are based on the B-lymphotropic Epstein-Barr virus (EBV), a human gamma-herpesvirus. This 293HEK-derived packaging cell line harbors a helper virus genome with a genetic modification that prevents the release of helper virions, but efficiently packages vector plasmids into virus-like particles with transducing capacity for human B cells. Here, we extended this basic approach towards a non-transforming, virus-free packaging cell line, which harbors an EBV helper virus genome with seven genetic alterations. In addition, we constructed a novel gene vector plasmid, which is devoid of a prokaryotic antibiotic resistance gene, and thus more suitable for in vivo applications in human gene therapy. We demonstrate in this paper that EBV-based gene vectors can be efficiently generated with this much-improved packaging cell line to provide helper virus-free gene vector stocks with transducing capacity for established human B-cell lines and primary B cells. More... »

PAGES

3302714

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3302714

DOI

http://dx.doi.org/10.1038/sj.gt.3302714

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009394578

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16421600


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1004", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Technology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "B-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Helper Viruses", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Herpesvirus 4, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombination, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Assembly", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Helmholtz Zentrum M\u00fcnchen", 
          "id": "https://www.grid.ac/institutes/grid.4567.0", 
          "name": [
            "Department of Gene Vectors, GSF-National Research Center for Environment and Health, M\u00fcnchen, Germany", 
            "Vaecgene Biotech GmbH, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hettich", 
        "givenName": "E", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Helmholtz Zentrum M\u00fcnchen", 
          "id": "https://www.grid.ac/institutes/grid.4567.0", 
          "name": [
            "Department of Gene Vectors, GSF-National Research Center for Environment and Health, M\u00fcnchen, Germany", 
            "Vaecgene Biotech GmbH, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Janz", 
        "givenName": "A", 
        "id": "sg:person.01155453623.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155453623.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Vaecgene Biotech GmbH, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zeidler", 
        "givenName": "R", 
        "id": "sg:person.01277516304.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277516304.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Helmholtz Zentrum M\u00fcnchen", 
          "id": "https://www.grid.ac/institutes/grid.4567.0", 
          "name": [
            "Department of Gene Vectors, GSF-National Research Center for Environment and Health, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pich", 
        "givenName": "D", 
        "id": "sg:person.01112665017.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112665017.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Helmholtz Zentrum M\u00fcnchen", 
          "id": "https://www.grid.ac/institutes/grid.4567.0", 
          "name": [
            "Department of Gene Vectors, GSF-National Research Center for Environment and Health, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hellebrand", 
        "givenName": "E", 
        "id": "sg:person.0603123445.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603123445.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwig Maximilian University of Munich", 
          "id": "https://www.grid.ac/institutes/grid.5252.0", 
          "name": [
            "Clinical Cooperation Group Molecular Oncology, Department of Otorhinolaryngology, GSF-National Research Center for Environment and Health, M\u00fcnchen, Germany", 
            "Ludwig-Maximilians-Universit\u00e4t, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weissflog", 
        "givenName": "B", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwig Maximilian University of Munich", 
          "id": "https://www.grid.ac/institutes/grid.5252.0", 
          "name": [
            "Clinical Cooperation Group Molecular Oncology, Department of Otorhinolaryngology, GSF-National Research Center for Environment and Health, M\u00fcnchen, Germany", 
            "Ludwig-Maximilians-Universit\u00e4t, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moosmann", 
        "givenName": "A", 
        "id": "sg:person.0703163511.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703163511.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Helmholtz Zentrum M\u00fcnchen", 
          "id": "https://www.grid.ac/institutes/grid.4567.0", 
          "name": [
            "Department of Gene Vectors, GSF-National Research Center for Environment and Health, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hammerschmidt", 
        "givenName": "W", 
        "id": "sg:person.01261032126.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261032126.15"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/emboj/17.6.1700", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002656129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.12.3080", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003638890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1066", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003654890", 
          "https://doi.org/10.1038/nrg1066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1066", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003654890", 
          "https://doi.org/10.1038/nrg1066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(88)90028-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005787145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/rmv.380", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005925481"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1101-1067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009031546", 
          "https://doi.org/10.1038/nbt1101-1067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1101-1067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009031546", 
          "https://doi.org/10.1038/nbt1101-1067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci119822", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009362656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.19.9150", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012160666"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.211556598", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012440482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-36-1-59", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012757655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/340393a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013091913", 
          "https://doi.org/10.1038/340393a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1994.1404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013969931"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/1-59259-848-x:353", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014113863", 
          "https://doi.org/10.1385/1-59259-848-x:353"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0030404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014610360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0030404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014610360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.13.5875", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016494013"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302602", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018433144", 
          "https://doi.org/10.1038/sj.gt.3302602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302602", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018433144", 
          "https://doi.org/10.1038/sj.gt.3302602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.76.19.9635-9644.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019023812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1985.tb00980.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020143931"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1985.tb00980.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020143931"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.9.5188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021640617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.79.12.7641-7647.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021887584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.217202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024343987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.20324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024606359"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.14.8245", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025621652"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.78.9.4783-4796.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027504871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1525-0016(03)00060-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028534468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1525-0016(03)00060-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028534468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.98.4.1865", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029792657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molmed.2004.05.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033741020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.21.10142-10152.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035258187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro958", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037056236", 
          "https://doi.org/10.1038/nrmicro958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro958", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037056236", 
          "https://doi.org/10.1038/nrmicro958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/1-59259-304-6:51", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037671024", 
          "https://doi.org/10.1385/1-59259-304-6:51"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(64)92345-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039319664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(64)92345-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039319664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/310207a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039528107", 
          "https://doi.org/10.1038/310207a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.2336099100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040171084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.182.5.1265", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041293582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.23.9558", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042678995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(02)01019-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043698919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.69.2.383", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047277981"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/83324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292282", 
          "https://doi.org/10.1038/83324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/83324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292282", 
          "https://doi.org/10.1038/83324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.232381299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048261088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0408774102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048477789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1208367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050182366", 
          "https://doi.org/10.1038/sj.onc.1208367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1208367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050182366", 
          "https://doi.org/10.1038/sj.onc.1208367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mthe.2002.0557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050423121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/1-59259-753-x:269", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052089439", 
          "https://doi.org/10.1385/1-59259-753-x:269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(02)46083-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053090341"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90070-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053396779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2660262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062549379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.179.13.4426-4428.1997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062726053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074952187", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075217452", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075295428", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076957067", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081654235", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082473919", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082594493", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082683040", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1993.tb06070.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082792664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082809695", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082893110", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb00674.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082902124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082957670", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083303016", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-05", 
    "datePublishedReg": "2006-05-01", 
    "description": "Viral gene vectors often rely on packaging cell lines, which provide the necessary factors in trans for the formation of virus-like particles. Previously, we reported on a first-generation packaging cell line for gene vectors, which are based on the B-lymphotropic Epstein-Barr virus (EBV), a human gamma-herpesvirus. This 293HEK-derived packaging cell line harbors a helper virus genome with a genetic modification that prevents the release of helper virions, but efficiently packages vector plasmids into virus-like particles with transducing capacity for human B cells. Here, we extended this basic approach towards a non-transforming, virus-free packaging cell line, which harbors an EBV helper virus genome with seven genetic alterations. In addition, we constructed a novel gene vector plasmid, which is devoid of a prokaryotic antibiotic resistance gene, and thus more suitable for in vivo applications in human gene therapy. We demonstrate in this paper that EBV-based gene vectors can be efficiently generated with this much-improved packaging cell line to provide helper virus-free gene vector stocks with transducing capacity for established human B-cell lines and primary B cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.gt.3302714", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2472659", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "name": "Genetic design of an optimized packaging cell line for gene vectors transducing human B cells", 
    "pagination": "3302714", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "2cc0aa37b603a1ff26864df70d78466f06881a9cf73c8332200294f2451aafdf"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16421600"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3302714"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1009394578"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3302714", 
      "https://app.dimensions.ai/details/publication/pub.1009394578"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:11", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53978_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/3302714"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302714'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302714'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302714'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302714'


 

This table displays all metadata directly associated to this object as RDF triples.

371 TRIPLES      21 PREDICATES      106 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3302714 schema:about N085aab66a772467fbdb6ac35a6016a0c
2 N0dc7c4b1ca864328bb69c9f8c4698dda
3 N1a18cfdff62a4b639fefac73968bab1e
4 N252f7173b04f46058bbb3ee5160ab4c4
5 N3e68cdf4461c4546b36d26c0bbc27658
6 N3faa1cd01c2648b8ad021f1bc76d0bea
7 N43ddbd2c78454f0793f158cd8fa7284a
8 N4b39df46e891476ab64952b37d3133d9
9 N6cbb776eca99465297dcf7aff82774a5
10 N6f09a0d4916c405891e6b09c7dc4271e
11 N7ed654d25ff449a6bbc2dbde71e9664f
12 Na8c9622cc68d49eebdf2eaeba7ded365
13 Nc5b5959ee6b14480aaab2d14c4cb8618
14 Nc8be260960fc4d5ba67812a95922b212
15 Nd9790b775d3a45b88e7c9fdea79ebe24
16 Ne5e29069f7234d6e8eb28b7963a9af57
17 anzsrc-for:10
18 anzsrc-for:1004
19 schema:author N3fa484f5d6a84df2aefaec72d5a737d9
20 schema:citation sg:pub.10.1038/310207a0
21 sg:pub.10.1038/340393a0
22 sg:pub.10.1038/83324
23 sg:pub.10.1038/nbt1101-1067
24 sg:pub.10.1038/nrg1066
25 sg:pub.10.1038/nrmicro958
26 sg:pub.10.1038/sj.gt.3302602
27 sg:pub.10.1038/sj.onc.1208367
28 sg:pub.10.1385/1-59259-304-6:51
29 sg:pub.10.1385/1-59259-753-x:269
30 sg:pub.10.1385/1-59259-848-x:353
31 https://app.dimensions.ai/details/publication/pub.1074952187
32 https://app.dimensions.ai/details/publication/pub.1075217452
33 https://app.dimensions.ai/details/publication/pub.1075295428
34 https://app.dimensions.ai/details/publication/pub.1076957067
35 https://app.dimensions.ai/details/publication/pub.1081654235
36 https://app.dimensions.ai/details/publication/pub.1082473919
37 https://app.dimensions.ai/details/publication/pub.1082594493
38 https://app.dimensions.ai/details/publication/pub.1082683040
39 https://app.dimensions.ai/details/publication/pub.1082809695
40 https://app.dimensions.ai/details/publication/pub.1082893110
41 https://app.dimensions.ai/details/publication/pub.1082957670
42 https://app.dimensions.ai/details/publication/pub.1083303016
43 https://doi.org/10.1002/j.1460-2075.1993.tb06070.x
44 https://doi.org/10.1002/j.1460-2075.1996.tb00674.x
45 https://doi.org/10.1002/jcb.20324
46 https://doi.org/10.1002/rmv.380
47 https://doi.org/10.1006/jmbi.1994.1404
48 https://doi.org/10.1006/mthe.2002.0557
49 https://doi.org/10.1016/0092-8674(88)90028-1
50 https://doi.org/10.1016/0092-8674(95)90070-5
51 https://doi.org/10.1016/j.molmed.2004.05.006
52 https://doi.org/10.1016/s0076-6879(02)46083-1
53 https://doi.org/10.1016/s0140-6736(64)92345-1
54 https://doi.org/10.1016/s0301-472x(02)01019-6
55 https://doi.org/10.1016/s1525-0016(03)00060-1
56 https://doi.org/10.1073/pnas.0408774102
57 https://doi.org/10.1073/pnas.211556598
58 https://doi.org/10.1073/pnas.232381299
59 https://doi.org/10.1073/pnas.2336099100
60 https://doi.org/10.1073/pnas.69.2.383
61 https://doi.org/10.1073/pnas.86.23.9558
62 https://doi.org/10.1073/pnas.90.19.9150
63 https://doi.org/10.1073/pnas.92.13.5875
64 https://doi.org/10.1073/pnas.95.14.8245
65 https://doi.org/10.1073/pnas.96.9.5188
66 https://doi.org/10.1073/pnas.98.4.1865
67 https://doi.org/10.1084/jem.182.5.1265
68 https://doi.org/10.1093/emboj/17.6.1700
69 https://doi.org/10.1093/emboj/19.12.3080
70 https://doi.org/10.1099/0022-1317-36-1-59
71 https://doi.org/10.1101/gr.217202
72 https://doi.org/10.1111/j.1574-6968.1985.tb00980.x
73 https://doi.org/10.1126/science.2660262
74 https://doi.org/10.1128/jb.179.13.4426-4428.1997
75 https://doi.org/10.1128/jvi.74.21.10142-10152.2000
76 https://doi.org/10.1128/jvi.76.19.9635-9644.2002
77 https://doi.org/10.1128/jvi.78.9.4783-4796.2004
78 https://doi.org/10.1128/jvi.79.12.7641-7647.2005
79 https://doi.org/10.1172/jci119822
80 https://doi.org/10.1371/journal.pbio.0030404
81 schema:datePublished 2006-05
82 schema:datePublishedReg 2006-05-01
83 schema:description Viral gene vectors often rely on packaging cell lines, which provide the necessary factors in trans for the formation of virus-like particles. Previously, we reported on a first-generation packaging cell line for gene vectors, which are based on the B-lymphotropic Epstein-Barr virus (EBV), a human gamma-herpesvirus. This 293HEK-derived packaging cell line harbors a helper virus genome with a genetic modification that prevents the release of helper virions, but efficiently packages vector plasmids into virus-like particles with transducing capacity for human B cells. Here, we extended this basic approach towards a non-transforming, virus-free packaging cell line, which harbors an EBV helper virus genome with seven genetic alterations. In addition, we constructed a novel gene vector plasmid, which is devoid of a prokaryotic antibiotic resistance gene, and thus more suitable for in vivo applications in human gene therapy. We demonstrate in this paper that EBV-based gene vectors can be efficiently generated with this much-improved packaging cell line to provide helper virus-free gene vector stocks with transducing capacity for established human B-cell lines and primary B cells.
84 schema:genre research_article
85 schema:inLanguage en
86 schema:isAccessibleForFree true
87 schema:isPartOf N9a9ac5bfc9a044948c8bbeeaa9ff5e90
88 Ncbee250b152c4949bb3b753ecea828df
89 sg:journal.1105638
90 schema:name Genetic design of an optimized packaging cell line for gene vectors transducing human B cells
91 schema:pagination 3302714
92 schema:productId N7bea5a05f54247abad9d69d25e7a7387
93 N8d1f4a30c17344f4a084908458f64368
94 Na57837f911e24144a4c619dd57830d72
95 Nc7831fc710e44795a6a6a6642a84788c
96 Nde686d77bb1a46ee90f9634ea3caae98
97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009394578
98 https://doi.org/10.1038/sj.gt.3302714
99 schema:sdDatePublished 2019-04-11T12:11
100 schema:sdLicense https://scigraph.springernature.com/explorer/license/
101 schema:sdPublisher N019d74839c3d41f087f396ad044e9439
102 schema:url http://www.nature.com/articles/3302714
103 sgo:license sg:explorer/license/
104 sgo:sdDataset articles
105 rdf:type schema:ScholarlyArticle
106 N019d74839c3d41f087f396ad044e9439 schema:name Springer Nature - SN SciGraph project
107 rdf:type schema:Organization
108 N085aab66a772467fbdb6ac35a6016a0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Cell Line
110 rdf:type schema:DefinedTerm
111 N0a24c3845e984962bf7d74c48b77aa6e schema:affiliation https://www.grid.ac/institutes/grid.4567.0
112 schema:familyName Hettich
113 schema:givenName E
114 rdf:type schema:Person
115 N0a60a7f3041944a0a8310d360dc30e9b rdf:first sg:person.0603123445.78
116 rdf:rest Nf4bf1cadc19a4d3bb14a09cc90bafdb1
117 N0dc7c4b1ca864328bb69c9f8c4698dda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Helper Viruses
119 rdf:type schema:DefinedTerm
120 N1a18cfdff62a4b639fefac73968bab1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Transduction, Genetic
122 rdf:type schema:DefinedTerm
123 N252f7173b04f46058bbb3ee5160ab4c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Gene Expression
125 rdf:type schema:DefinedTerm
126 N333e650347784e7f9d3163c966ae9295 rdf:first sg:person.0703163511.84
127 rdf:rest N84687e1a50e44919a84527d8c4bd2b13
128 N3e68cdf4461c4546b36d26c0bbc27658 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name B-Lymphocytes
130 rdf:type schema:DefinedTerm
131 N3fa484f5d6a84df2aefaec72d5a737d9 rdf:first N0a24c3845e984962bf7d74c48b77aa6e
132 rdf:rest Na020364de4234784b64d867fd8664470
133 N3faa1cd01c2648b8ad021f1bc76d0bea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Herpesvirus 4, Human
135 rdf:type schema:DefinedTerm
136 N43ddbd2c78454f0793f158cd8fa7284a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Transfection
138 rdf:type schema:DefinedTerm
139 N4b39df46e891476ab64952b37d3133d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Humans
141 rdf:type schema:DefinedTerm
142 N605f604e47e441ca815b73eef796dff5 schema:name Vaecgene Biotech GmbH, München, Germany
143 rdf:type schema:Organization
144 N65b7ed96f0fc43a0ac8c96d38cc25ae7 schema:affiliation https://www.grid.ac/institutes/grid.5252.0
145 schema:familyName Weissflog
146 schema:givenName B
147 rdf:type schema:Person
148 N6cbb776eca99465297dcf7aff82774a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name DNA, Viral
150 rdf:type schema:DefinedTerm
151 N6f09a0d4916c405891e6b09c7dc4271e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Green Fluorescent Proteins
153 rdf:type schema:DefinedTerm
154 N791704ca512c4749b5e825156b65e9b0 rdf:first sg:person.01112665017.08
155 rdf:rest N0a60a7f3041944a0a8310d360dc30e9b
156 N7bea5a05f54247abad9d69d25e7a7387 schema:name dimensions_id
157 schema:value pub.1009394578
158 rdf:type schema:PropertyValue
159 N7ed654d25ff449a6bbc2dbde71e9664f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Flow Cytometry
161 rdf:type schema:DefinedTerm
162 N84687e1a50e44919a84527d8c4bd2b13 rdf:first sg:person.01261032126.15
163 rdf:rest rdf:nil
164 N8d1f4a30c17344f4a084908458f64368 schema:name doi
165 schema:value 10.1038/sj.gt.3302714
166 rdf:type schema:PropertyValue
167 N9a9ac5bfc9a044948c8bbeeaa9ff5e90 schema:volumeNumber 13
168 rdf:type schema:PublicationVolume
169 Na020364de4234784b64d867fd8664470 rdf:first sg:person.01155453623.79
170 rdf:rest Ne8aef9b937784d23a2e9bb33fd2f22eb
171 Na57837f911e24144a4c619dd57830d72 schema:name pubmed_id
172 schema:value 16421600
173 rdf:type schema:PropertyValue
174 Na8c9622cc68d49eebdf2eaeba7ded365 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Recombination, Genetic
176 rdf:type schema:DefinedTerm
177 Nc5b5959ee6b14480aaab2d14c4cb8618 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Genetic Engineering
179 rdf:type schema:DefinedTerm
180 Nc7831fc710e44795a6a6a6642a84788c schema:name readcube_id
181 schema:value 2cc0aa37b603a1ff26864df70d78466f06881a9cf73c8332200294f2451aafdf
182 rdf:type schema:PropertyValue
183 Nc8be260960fc4d5ba67812a95922b212 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Virus Assembly
185 rdf:type schema:DefinedTerm
186 Ncbee250b152c4949bb3b753ecea828df schema:issueNumber 10
187 rdf:type schema:PublicationIssue
188 Nd9790b775d3a45b88e7c9fdea79ebe24 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Polymerase Chain Reaction
190 rdf:type schema:DefinedTerm
191 Nde686d77bb1a46ee90f9634ea3caae98 schema:name nlm_unique_id
192 schema:value 9421525
193 rdf:type schema:PropertyValue
194 Ne5e29069f7234d6e8eb28b7963a9af57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Genetic Vectors
196 rdf:type schema:DefinedTerm
197 Ne8aef9b937784d23a2e9bb33fd2f22eb rdf:first sg:person.01277516304.27
198 rdf:rest N791704ca512c4749b5e825156b65e9b0
199 Nf4bf1cadc19a4d3bb14a09cc90bafdb1 rdf:first N65b7ed96f0fc43a0ac8c96d38cc25ae7
200 rdf:rest N333e650347784e7f9d3163c966ae9295
201 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
202 schema:name Technology
203 rdf:type schema:DefinedTerm
204 anzsrc-for:1004 schema:inDefinedTermSet anzsrc-for:
205 schema:name Medical Biotechnology
206 rdf:type schema:DefinedTerm
207 sg:grant.2472659 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3302714
208 rdf:type schema:MonetaryGrant
209 sg:journal.1105638 schema:issn 0969-7128
210 1476-5462
211 schema:name Gene Therapy
212 rdf:type schema:Periodical
213 sg:person.01112665017.08 schema:affiliation https://www.grid.ac/institutes/grid.4567.0
214 schema:familyName Pich
215 schema:givenName D
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112665017.08
217 rdf:type schema:Person
218 sg:person.01155453623.79 schema:affiliation https://www.grid.ac/institutes/grid.4567.0
219 schema:familyName Janz
220 schema:givenName A
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155453623.79
222 rdf:type schema:Person
223 sg:person.01261032126.15 schema:affiliation https://www.grid.ac/institutes/grid.4567.0
224 schema:familyName Hammerschmidt
225 schema:givenName W
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261032126.15
227 rdf:type schema:Person
228 sg:person.01277516304.27 schema:affiliation N605f604e47e441ca815b73eef796dff5
229 schema:familyName Zeidler
230 schema:givenName R
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277516304.27
232 rdf:type schema:Person
233 sg:person.0603123445.78 schema:affiliation https://www.grid.ac/institutes/grid.4567.0
234 schema:familyName Hellebrand
235 schema:givenName E
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603123445.78
237 rdf:type schema:Person
238 sg:person.0703163511.84 schema:affiliation https://www.grid.ac/institutes/grid.5252.0
239 schema:familyName Moosmann
240 schema:givenName A
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703163511.84
242 rdf:type schema:Person
243 sg:pub.10.1038/310207a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039528107
244 https://doi.org/10.1038/310207a0
245 rdf:type schema:CreativeWork
246 sg:pub.10.1038/340393a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013091913
247 https://doi.org/10.1038/340393a0
248 rdf:type schema:CreativeWork
249 sg:pub.10.1038/83324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047292282
250 https://doi.org/10.1038/83324
251 rdf:type schema:CreativeWork
252 sg:pub.10.1038/nbt1101-1067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009031546
253 https://doi.org/10.1038/nbt1101-1067
254 rdf:type schema:CreativeWork
255 sg:pub.10.1038/nrg1066 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003654890
256 https://doi.org/10.1038/nrg1066
257 rdf:type schema:CreativeWork
258 sg:pub.10.1038/nrmicro958 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037056236
259 https://doi.org/10.1038/nrmicro958
260 rdf:type schema:CreativeWork
261 sg:pub.10.1038/sj.gt.3302602 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018433144
262 https://doi.org/10.1038/sj.gt.3302602
263 rdf:type schema:CreativeWork
264 sg:pub.10.1038/sj.onc.1208367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050182366
265 https://doi.org/10.1038/sj.onc.1208367
266 rdf:type schema:CreativeWork
267 sg:pub.10.1385/1-59259-304-6:51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037671024
268 https://doi.org/10.1385/1-59259-304-6:51
269 rdf:type schema:CreativeWork
270 sg:pub.10.1385/1-59259-753-x:269 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052089439
271 https://doi.org/10.1385/1-59259-753-x:269
272 rdf:type schema:CreativeWork
273 sg:pub.10.1385/1-59259-848-x:353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014113863
274 https://doi.org/10.1385/1-59259-848-x:353
275 rdf:type schema:CreativeWork
276 https://app.dimensions.ai/details/publication/pub.1074952187 schema:CreativeWork
277 https://app.dimensions.ai/details/publication/pub.1075217452 schema:CreativeWork
278 https://app.dimensions.ai/details/publication/pub.1075295428 schema:CreativeWork
279 https://app.dimensions.ai/details/publication/pub.1076957067 schema:CreativeWork
280 https://app.dimensions.ai/details/publication/pub.1081654235 schema:CreativeWork
281 https://app.dimensions.ai/details/publication/pub.1082473919 schema:CreativeWork
282 https://app.dimensions.ai/details/publication/pub.1082594493 schema:CreativeWork
283 https://app.dimensions.ai/details/publication/pub.1082683040 schema:CreativeWork
284 https://app.dimensions.ai/details/publication/pub.1082809695 schema:CreativeWork
285 https://app.dimensions.ai/details/publication/pub.1082893110 schema:CreativeWork
286 https://app.dimensions.ai/details/publication/pub.1082957670 schema:CreativeWork
287 https://app.dimensions.ai/details/publication/pub.1083303016 schema:CreativeWork
288 https://doi.org/10.1002/j.1460-2075.1993.tb06070.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082792664
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1002/j.1460-2075.1996.tb00674.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082902124
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1002/jcb.20324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024606359
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1002/rmv.380 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005925481
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1006/jmbi.1994.1404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013969931
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1006/mthe.2002.0557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050423121
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1016/0092-8674(88)90028-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005787145
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1016/0092-8674(95)90070-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053396779
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1016/j.molmed.2004.05.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033741020
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1016/s0076-6879(02)46083-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053090341
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1016/s0140-6736(64)92345-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039319664
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1016/s0301-472x(02)01019-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043698919
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1016/s1525-0016(03)00060-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028534468
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1073/pnas.0408774102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048477789
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1073/pnas.211556598 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012440482
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1073/pnas.232381299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048261088
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1073/pnas.2336099100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040171084
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1073/pnas.69.2.383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047277981
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1073/pnas.86.23.9558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042678995
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1073/pnas.90.19.9150 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012160666
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1073/pnas.92.13.5875 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016494013
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1073/pnas.95.14.8245 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025621652
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1073/pnas.96.9.5188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021640617
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1073/pnas.98.4.1865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029792657
335 rdf:type schema:CreativeWork
336 https://doi.org/10.1084/jem.182.5.1265 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041293582
337 rdf:type schema:CreativeWork
338 https://doi.org/10.1093/emboj/17.6.1700 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002656129
339 rdf:type schema:CreativeWork
340 https://doi.org/10.1093/emboj/19.12.3080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003638890
341 rdf:type schema:CreativeWork
342 https://doi.org/10.1099/0022-1317-36-1-59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012757655
343 rdf:type schema:CreativeWork
344 https://doi.org/10.1101/gr.217202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024343987
345 rdf:type schema:CreativeWork
346 https://doi.org/10.1111/j.1574-6968.1985.tb00980.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020143931
347 rdf:type schema:CreativeWork
348 https://doi.org/10.1126/science.2660262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062549379
349 rdf:type schema:CreativeWork
350 https://doi.org/10.1128/jb.179.13.4426-4428.1997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062726053
351 rdf:type schema:CreativeWork
352 https://doi.org/10.1128/jvi.74.21.10142-10152.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035258187
353 rdf:type schema:CreativeWork
354 https://doi.org/10.1128/jvi.76.19.9635-9644.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019023812
355 rdf:type schema:CreativeWork
356 https://doi.org/10.1128/jvi.78.9.4783-4796.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027504871
357 rdf:type schema:CreativeWork
358 https://doi.org/10.1128/jvi.79.12.7641-7647.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021887584
359 rdf:type schema:CreativeWork
360 https://doi.org/10.1172/jci119822 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009362656
361 rdf:type schema:CreativeWork
362 https://doi.org/10.1371/journal.pbio.0030404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014610360
363 rdf:type schema:CreativeWork
364 https://www.grid.ac/institutes/grid.4567.0 schema:alternateName Helmholtz Zentrum München
365 schema:name Department of Gene Vectors, GSF-National Research Center for Environment and Health, München, Germany
366 Vaecgene Biotech GmbH, München, Germany
367 rdf:type schema:Organization
368 https://www.grid.ac/institutes/grid.5252.0 schema:alternateName Ludwig Maximilian University of Munich
369 schema:name Clinical Cooperation Group Molecular Oncology, Department of Otorhinolaryngology, GSF-National Research Center for Environment and Health, München, Germany
370 Ludwig-Maximilians-Universität, München, Germany
371 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...