Genetic design of an optimized packaging cell line for gene vectors transducing human B cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-05

AUTHORS

E Hettich, A Janz, R Zeidler, D Pich, E Hellebrand, B Weissflog, A Moosmann, W Hammerschmidt

ABSTRACT

Viral gene vectors often rely on packaging cell lines, which provide the necessary factors in trans for the formation of virus-like particles. Previously, we reported on a first-generation packaging cell line for gene vectors, which are based on the B-lymphotropic Epstein-Barr virus (EBV), a human gamma-herpesvirus. This 293HEK-derived packaging cell line harbors a helper virus genome with a genetic modification that prevents the release of helper virions, but efficiently packages vector plasmids into virus-like particles with transducing capacity for human B cells. Here, we extended this basic approach towards a non-transforming, virus-free packaging cell line, which harbors an EBV helper virus genome with seven genetic alterations. In addition, we constructed a novel gene vector plasmid, which is devoid of a prokaryotic antibiotic resistance gene, and thus more suitable for in vivo applications in human gene therapy. We demonstrate in this paper that EBV-based gene vectors can be efficiently generated with this much-improved packaging cell line to provide helper virus-free gene vector stocks with transducing capacity for established human B-cell lines and primary B cells. More... »

PAGES

3302714

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3302714

DOI

http://dx.doi.org/10.1038/sj.gt.3302714

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009394578

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16421600


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