Ontology type: schema:ScholarlyArticle
2006-01-05
AUTHORSY Sugiyama-Nakagiri, M Akiyama, H Shimizu
ABSTRACTGene transfer to hair follicle (HF) epithelium is an attractive approach for not only treating skin diseases, but also many systemic disorders. In this study, we attempted to develop a gene transfer system for HF epithelial stem cells to maximize the beneficial therapeutic effects. For persistent and stable transgene expression in HF stem cells, we transferred retroviral vectors encoding reporter genes into cultured HF stem cells. In addition, these cells were mixed with cultured dermal papilla cells and transplanted on to immunodeficient mice. We succeeded in reconstituting HFs and their appendages in which these cells harbored a transgene reporter. The transgene expression was observed in all skin epithelial compartments including the HF epithelium, sebaceous gland and epidermis. In addition, transgene expression was observed for at least 6 months. This HF stem cell-targeted gene transfer and reconstitution system provides reliable gene-function analysis and gene therapy. More... »
PAGES732-737
http://scigraph.springernature.com/pub.10.1038/sj.gt.3302709
DOIhttp://dx.doi.org/10.1038/sj.gt.3302709
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1002500284
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/16397506
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cells, Cultured",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Epithelial Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Therapy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Vectors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Green Fluorescent Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hair Follicle",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, SCID",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microscopy, Electron, Scanning",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Oligonucleotide Array Sequence Analysis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Regeneration",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Retroviridae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Skin Diseases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Stem Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transduction, Genetic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transgenes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Vibrissae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "beta-Galactosidase",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan",
"id": "http://www.grid.ac/institutes/grid.39158.36",
"name": [
"Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan"
],
"type": "Organization"
},
"familyName": "Sugiyama-Nakagiri",
"givenName": "Y",
"id": "sg:person.0603721461.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603721461.06"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan",
"id": "http://www.grid.ac/institutes/grid.39158.36",
"name": [
"Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan"
],
"type": "Organization"
},
"familyName": "Akiyama",
"givenName": "M",
"id": "sg:person.013324706103.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013324706103.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan",
"id": "http://www.grid.ac/institutes/grid.39158.36",
"name": [
"Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan"
],
"type": "Organization"
},
"familyName": "Shimizu",
"givenName": "H",
"id": "sg:person.013436151371.64",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013436151371.64"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/sj.gt.3302264",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002525020",
"https://doi.org/10.1038/sj.gt.3302264"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt950",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050242098",
"https://doi.org/10.1038/nbt950"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-3-642-84881-0_3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036497013",
"https://doi.org/10.1007/978-3-642-84881-0_3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3300956",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028166325",
"https://doi.org/10.1038/sj.gt.3300956"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nm0795-705",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012231398",
"https://doi.org/10.1038/nm0795-705"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/74480",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023765754",
"https://doi.org/10.1038/74480"
],
"type": "CreativeWork"
}
],
"datePublished": "2006-01-05",
"datePublishedReg": "2006-01-05",
"description": "Gene transfer to hair follicle (HF) epithelium is an attractive approach for not only treating skin diseases, but also many systemic disorders. In this study, we attempted to develop a gene transfer system for HF epithelial stem cells to maximize the beneficial therapeutic effects. For persistent and stable transgene expression in HF stem cells, we transferred retroviral vectors encoding reporter genes into cultured HF stem cells. In addition, these cells were mixed with cultured dermal papilla cells and transplanted on to immunodeficient mice. We succeeded in reconstituting HFs and their appendages in which these cells harbored a transgene reporter. The transgene expression was observed in all skin epithelial compartments including the HF epithelium, sebaceous gland and epidermis. In addition, transgene expression was observed for at least 6 months. This HF stem cell-targeted gene transfer and reconstitution system provides reliable gene-function analysis and gene therapy.",
"genre": "article",
"id": "sg:pub.10.1038/sj.gt.3302709",
"inLanguage": "en",
"isAccessibleForFree": false,
"isFundedItemOf": [
{
"id": "sg:grant.5886312",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1105638",
"issn": [
"0969-7128",
"1476-5462"
],
"name": "Gene Therapy",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "8",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "13"
}
],
"keywords": [
"transgene expression",
"gene transfer",
"stable transgene expression",
"gene transfer system",
"HF epithelial stem cells",
"stem cells",
"gene function analysis",
"gene therapy",
"retroviral vectors",
"HF stem cells",
"attractive approach",
"transgene reporter",
"transfer system",
"epithelial stem cells",
"reporter gene",
"HFS",
"transfer",
"beneficial therapeutic effects",
"cultured dermal papilla cells",
"cells",
"immunodeficient mice",
"system",
"therapeutic effect",
"hair follicle epithelium",
"vector",
"systemic disorders",
"dermal papilla cells",
"HF epithelium",
"skin diseases",
"reconstitution system",
"sebaceous glands",
"epithelial compartment",
"follicle epithelium",
"papilla cells",
"addition",
"reporter",
"epithelium",
"expression",
"therapy",
"approach",
"disease",
"mice",
"months",
"genes",
"disorders",
"gland",
"epidermis",
"effect",
"compartments",
"analysis",
"appendages",
"study"
],
"name": "Hair follicle stem cell-targeted gene transfer and reconstitution system",
"pagination": "732-737",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1002500284"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/sj.gt.3302709"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"16397506"
]
}
],
"sameAs": [
"https://doi.org/10.1038/sj.gt.3302709",
"https://app.dimensions.ai/details/publication/pub.1002500284"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:23",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_426.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/sj.gt.3302709"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302709'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302709'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302709'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302709'
This table displays all metadata directly associated to this object as RDF triples.
238 TRIPLES
22 PREDICATES
105 URIs
91 LITERALS
28 BLANK NODES