Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-09-22

AUTHORS

Y D Kim, K-G Park, R Morishita, Y Kaneda, S-Y Kim, D-K Song, H-S Kim, C-W Nam, H C Lee, K-U Lee, J-Y Park, B-W Kim, J-G Kim, I-K Lee

ABSTRACT

Insulin gene therapy in clinical medicine is currently hampered by the inability to regulate insulin secretion in a physiological manner, the inefficiency with which the gene is delivered, and the short duration of gene expression. To address these issues, we injected the liver of streptozotocin-induced diabetic rats with hemagglutinating virus of Japan-envelope (HVJ-E) vectors containing Epstein–Barr virus (EBV) plasmids encoding the genes for insulin and the GLUT 2 transporter. Efficient delivery of the genes was achieved with the HVJ-E vector, and the use of the EBV replicon vector led to prolonged hepatic gene expression. Blood glucose levels were normalized for at least 3 weeks as a result of the gene therapy. Cotransfection of GLUT 2 with insulin permitted the diabetic rats to regulate their blood glucose levels upon exogenous glucose loading in a physiologically appropriate manner and improved postprandial glucose levels. Moreover, cotransfection with insulin and GLUT 2 genes led to in vitro glucose-stimulated insulin secretion that involved the closure of KATP channels. The present study represents a new way to efficiently deliver insulin gene in vivo that is regulated by ambient glucose level with prolonged gene expression. This may provide a basis to overcome limitations of insulin gene therapy in humans. More... »

PAGES

216-224

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3302644

DOI

http://dx.doi.org/10.1038/sj.gt.3302644

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022857120

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16177820


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diabetes Mellitus, Experimental", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucose Transporter Type 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Herpesvirus 4, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Insulin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Potassium Channels", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats, Sprague-Dawley", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sendai virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Envelope Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.412091.f", 
          "name": [
            "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Y D", 
        "id": "sg:person.01056176550.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056176550.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.412091.f", 
          "name": [
            "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Park", 
        "givenName": "K-G", 
        "id": "sg:person.0672231035.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672231035.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Morishita", 
        "givenName": "R", 
        "id": "sg:person.01334645231.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334645231.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kaneda", 
        "givenName": "Y", 
        "id": "sg:person.01241111761.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241111761.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.412091.f", 
          "name": [
            "Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "S-Y", 
        "id": "sg:person.01175446071.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175446071.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.412091.f", 
          "name": [
            "Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "D-K", 
        "id": "sg:person.01307574076.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307574076.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.412091.f", 
          "name": [
            "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "H-S", 
        "id": "sg:person.01261450260.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261450260.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.412091.f", 
          "name": [
            "Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nam", 
        "givenName": "C-W", 
        "id": "sg:person.01313752601.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313752601.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.15444.30", 
          "name": [
            "Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "H C", 
        "id": "sg:person.01163711676.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163711676.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.267370.7", 
          "name": [
            "Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "K-U", 
        "id": "sg:person.01036122220.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036122220.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.267370.7", 
          "name": [
            "Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Park", 
        "givenName": "J-Y", 
        "id": "sg:person.014104715634.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014104715634.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.258803.4", 
          "name": [
            "Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "B-W", 
        "id": "sg:person.01354732600.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354732600.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.258803.4", 
          "name": [
            "Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "J-G", 
        "id": "sg:person.0624115635.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624115635.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea", 
          "id": "http://www.grid.ac/institutes/grid.258803.4", 
          "name": [
            "Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "I-K", 
        "id": "sg:person.015663503347.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015663503347.24"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/sj.gt.3300711", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031761115", 
          "https://doi.org/10.1038/sj.gt.3300711"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302412", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013480844", 
          "https://doi.org/10.1038/sj.gt.3302412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35044106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021678417", 
          "https://doi.org/10.1038/35044106"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/313812a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021158149", 
          "https://doi.org/10.1038/313812a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302236", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027361283", 
          "https://doi.org/10.1038/sj.gt.3302236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301849", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048568841", 
          "https://doi.org/10.1038/sj.gt.3301849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/357455a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044352249", 
          "https://doi.org/10.1038/357455a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/83324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292282", 
          "https://doi.org/10.1038/83324"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2005-09-22", 
    "datePublishedReg": "2005-09-22", 
    "description": "Insulin gene therapy in clinical medicine is currently hampered by the inability to regulate insulin secretion in a physiological manner, the inefficiency with which the gene is delivered, and the short duration of gene expression. To address these issues, we injected the liver of streptozotocin-induced diabetic rats with hemagglutinating virus of Japan-envelope (HVJ-E) vectors containing Epstein\u2013Barr virus (EBV) plasmids encoding the genes for insulin and the GLUT 2 transporter. Efficient delivery of the genes was achieved with the HVJ-E vector, and the use of the EBV replicon vector led to prolonged hepatic gene expression. Blood glucose levels were normalized for at least 3 weeks as a result of the gene therapy. Cotransfection of GLUT 2 with insulin permitted the diabetic rats to regulate their blood glucose levels upon exogenous glucose loading in a physiologically appropriate manner and improved postprandial glucose levels. Moreover, cotransfection with insulin and GLUT 2 genes led to in vitro glucose-stimulated insulin secretion that involved the closure of KATP channels. The present study represents a new way to efficiently deliver insulin gene in vivo that is regulated by ambient glucose level with prolonged gene expression. This may provide a basis to overcome limitations of insulin gene therapy in humans.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/sj.gt.3302644", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "keywords": [
      "GLUT 2 transporter", 
      "blood glucose levels", 
      "glucose levels", 
      "diabetic rats", 
      "insulin gene therapy", 
      "insulin secretion", 
      "vitro glucose-stimulated insulin secretion", 
      "glucose-stimulated insulin secretion", 
      "postprandial glucose levels", 
      "liver of streptozotocin", 
      "gene therapy", 
      "ambient glucose levels", 
      "Japan-envelope vector", 
      "hepatic gene expression", 
      "glucose loading", 
      "KATP channels", 
      "EBV plasmids", 
      "gene expression", 
      "liver-directed gene therapy", 
      "therapy", 
      "insulin", 
      "GLUT 2", 
      "rats", 
      "GLUT 2 gene", 
      "physiological manner", 
      "short duration", 
      "replicon vector", 
      "virus plasmid", 
      "insulin gene", 
      "clinical medicine", 
      "secretion", 
      "present study", 
      "HVJ", 
      "expression", 
      "streptozotocin", 
      "levels", 
      "transporters", 
      "weeks", 
      "liver", 
      "genes", 
      "prolonged gene expression", 
      "virus", 
      "cotransfection", 
      "duration", 
      "vivo", 
      "efficient delivery", 
      "medicine", 
      "delivery", 
      "manner", 
      "humans", 
      "plasmid", 
      "closure", 
      "inability", 
      "appropriate manner", 
      "study", 
      "use", 
      "results", 
      "vector", 
      "limitations", 
      "basis", 
      "channels", 
      "issues", 
      "new way", 
      "loading", 
      "way", 
      "inefficiency", 
      "Epstein\u2013Barr virus (EBV) plasmids", 
      "EBV replicon vector", 
      "prolonged hepatic gene expression", 
      "exogenous glucose loading"
    ], 
    "name": "Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter", 
    "pagination": "216-224", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1022857120"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3302644"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16177820"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3302644", 
      "https://app.dimensions.ai/details/publication/pub.1022857120"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_408.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/sj.gt.3302644"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302644'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302644'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302644'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3302644'


 

This table displays all metadata directly associated to this object as RDF triples.

337 TRIPLES      22 PREDICATES      121 URIs      105 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3302644 schema:about N098756a92f494c7da3b0fd7d5880ab05
2 N0c3d8b2a0fe64c35aa1afa608da48a48
3 N17736d0b2adf469b831c58912ff4e89d
4 N2731351c8e1c414abdef5e25232c4ebe
5 N414bcb5562a149829e35434527ca4b22
6 N54fa5d9aae0744daae3d41cf78c22cab
7 N73cfcb0ce07c43cab17b62e7358326e3
8 N7b1034407b24457fa07d8b3ea53ff87d
9 N842c3fecc5034865a943f15081b8c229
10 N848c9a7325fc4e1b9da2b6a099d532de
11 N885b650f52c14c6b972fd9f6e4d09dae
12 Na0fee108ecca4e8fbeb2d7f352177029
13 Naa39060f30c6453bb2316c12d7b865d1
14 Nacbac982e74c4467a7eea6a287af74dc
15 Nce2e6cff60d84da38fa490b5707c8e07
16 Nf11e473414cc4d9c92f87b93272c42cd
17 Nf18df6dff93b47d190a8ac558bd5ed74
18 anzsrc-for:11
19 anzsrc-for:1103
20 schema:author Nd329c55507ac44d7b43552ff4e6e2fea
21 schema:citation sg:pub.10.1038/313812a0
22 sg:pub.10.1038/35044106
23 sg:pub.10.1038/357455a0
24 sg:pub.10.1038/83324
25 sg:pub.10.1038/sj.gt.3300711
26 sg:pub.10.1038/sj.gt.3301849
27 sg:pub.10.1038/sj.gt.3302236
28 sg:pub.10.1038/sj.gt.3302412
29 schema:datePublished 2005-09-22
30 schema:datePublishedReg 2005-09-22
31 schema:description Insulin gene therapy in clinical medicine is currently hampered by the inability to regulate insulin secretion in a physiological manner, the inefficiency with which the gene is delivered, and the short duration of gene expression. To address these issues, we injected the liver of streptozotocin-induced diabetic rats with hemagglutinating virus of Japan-envelope (HVJ-E) vectors containing Epstein–Barr virus (EBV) plasmids encoding the genes for insulin and the GLUT 2 transporter. Efficient delivery of the genes was achieved with the HVJ-E vector, and the use of the EBV replicon vector led to prolonged hepatic gene expression. Blood glucose levels were normalized for at least 3 weeks as a result of the gene therapy. Cotransfection of GLUT 2 with insulin permitted the diabetic rats to regulate their blood glucose levels upon exogenous glucose loading in a physiologically appropriate manner and improved postprandial glucose levels. Moreover, cotransfection with insulin and GLUT 2 genes led to in vitro glucose-stimulated insulin secretion that involved the closure of KATP channels. The present study represents a new way to efficiently deliver insulin gene in vivo that is regulated by ambient glucose level with prolonged gene expression. This may provide a basis to overcome limitations of insulin gene therapy in humans.
32 schema:genre article
33 schema:inLanguage en
34 schema:isAccessibleForFree true
35 schema:isPartOf N05efa950a2f84efd8f94adf225026b21
36 Nb5d42b7a1ccc48489d604ec9de082548
37 sg:journal.1105638
38 schema:keywords EBV plasmids
39 EBV replicon vector
40 Epstein–Barr virus (EBV) plasmids
41 GLUT 2
42 GLUT 2 gene
43 GLUT 2 transporter
44 HVJ
45 Japan-envelope vector
46 KATP channels
47 ambient glucose levels
48 appropriate manner
49 basis
50 blood glucose levels
51 channels
52 clinical medicine
53 closure
54 cotransfection
55 delivery
56 diabetic rats
57 duration
58 efficient delivery
59 exogenous glucose loading
60 expression
61 gene expression
62 gene therapy
63 genes
64 glucose levels
65 glucose loading
66 glucose-stimulated insulin secretion
67 hepatic gene expression
68 humans
69 inability
70 inefficiency
71 insulin
72 insulin gene
73 insulin gene therapy
74 insulin secretion
75 issues
76 levels
77 limitations
78 liver
79 liver of streptozotocin
80 liver-directed gene therapy
81 loading
82 manner
83 medicine
84 new way
85 physiological manner
86 plasmid
87 postprandial glucose levels
88 present study
89 prolonged gene expression
90 prolonged hepatic gene expression
91 rats
92 replicon vector
93 results
94 secretion
95 short duration
96 streptozotocin
97 study
98 therapy
99 transporters
100 use
101 vector
102 virus
103 virus plasmid
104 vitro glucose-stimulated insulin secretion
105 vivo
106 way
107 weeks
108 schema:name Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter
109 schema:pagination 216-224
110 schema:productId N65871e936c46435c8a3ae47cd459b7bb
111 Nc1247f2804ec41b3bdf3406ee2c83d3f
112 Nd172b79d254e4514a67f5c900f602915
113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022857120
114 https://doi.org/10.1038/sj.gt.3302644
115 schema:sdDatePublished 2021-11-01T18:08
116 schema:sdLicense https://scigraph.springernature.com/explorer/license/
117 schema:sdPublisher Nd8eebb8605ba4770a4dec8b9e4aacd19
118 schema:url https://doi.org/10.1038/sj.gt.3302644
119 sgo:license sg:explorer/license/
120 sgo:sdDataset articles
121 rdf:type schema:ScholarlyArticle
122 N05efa950a2f84efd8f94adf225026b21 schema:volumeNumber 13
123 rdf:type schema:PublicationVolume
124 N098756a92f494c7da3b0fd7d5880ab05 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Liver
126 rdf:type schema:DefinedTerm
127 N0c3d8b2a0fe64c35aa1afa608da48a48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Insulin
129 rdf:type schema:DefinedTerm
130 N110c89fa1615439cab9ed43777dbce32 rdf:first sg:person.01334645231.38
131 rdf:rest N16759519b07141559e7bbcfb1cfa06a5
132 N16759519b07141559e7bbcfb1cfa06a5 rdf:first sg:person.01241111761.53
133 rdf:rest N16ef03877b21474b9f005379346d0b4e
134 N16ef03877b21474b9f005379346d0b4e rdf:first sg:person.01175446071.86
135 rdf:rest N83a31342ac654c68bfd5be3e3d6393f7
136 N17736d0b2adf469b831c58912ff4e89d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Glucose Transporter Type 2
138 rdf:type schema:DefinedTerm
139 N2731351c8e1c414abdef5e25232c4ebe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Genetic Vectors
141 rdf:type schema:DefinedTerm
142 N2c8f82733ac647eb99e6287d66b050d8 rdf:first sg:person.01354732600.15
143 rdf:rest N4f9e4c49e205461b97f98805221be73c
144 N31173fd297574d9e9fd2c27d9a225202 rdf:first sg:person.01163711676.37
145 rdf:rest Neb61120614ce4a8e9b082a16c69a2328
146 N414bcb5562a149829e35434527ca4b22 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Male
148 rdf:type schema:DefinedTerm
149 N42d13e99a94d4b70b10ac628502905ae rdf:first sg:person.0672231035.77
150 rdf:rest N110c89fa1615439cab9ed43777dbce32
151 N4f9e4c49e205461b97f98805221be73c rdf:first sg:person.0624115635.98
152 rdf:rest Nbc8456f437a541f190012d02c9b49f85
153 N54fa5d9aae0744daae3d41cf78c22cab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Potassium Channels
155 rdf:type schema:DefinedTerm
156 N64edd06bf23547c0b8081de94ae00530 rdf:first sg:person.01313752601.46
157 rdf:rest N31173fd297574d9e9fd2c27d9a225202
158 N65871e936c46435c8a3ae47cd459b7bb schema:name dimensions_id
159 schema:value pub.1022857120
160 rdf:type schema:PropertyValue
161 N73cfcb0ce07c43cab17b62e7358326e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Rats, Sprague-Dawley
163 rdf:type schema:DefinedTerm
164 N7b1034407b24457fa07d8b3ea53ff87d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Sendai virus
166 rdf:type schema:DefinedTerm
167 N7d0cef4164ec452e979d7f5c81b637c7 rdf:first sg:person.014104715634.26
168 rdf:rest N2c8f82733ac647eb99e6287d66b050d8
169 N83a31342ac654c68bfd5be3e3d6393f7 rdf:first sg:person.01307574076.07
170 rdf:rest Ncdbb9a9fb6ec42448f8b92e22bbf3175
171 N842c3fecc5034865a943f15081b8c229 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Transduction, Genetic
173 rdf:type schema:DefinedTerm
174 N848c9a7325fc4e1b9da2b6a099d532de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Rats
176 rdf:type schema:DefinedTerm
177 N885b650f52c14c6b972fd9f6e4d09dae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Gene Expression
179 rdf:type schema:DefinedTerm
180 Na0fee108ecca4e8fbeb2d7f352177029 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Time Factors
182 rdf:type schema:DefinedTerm
183 Naa39060f30c6453bb2316c12d7b865d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Viral Envelope Proteins
185 rdf:type schema:DefinedTerm
186 Nacbac982e74c4467a7eea6a287af74dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Diabetes Mellitus, Experimental
188 rdf:type schema:DefinedTerm
189 Nb5d42b7a1ccc48489d604ec9de082548 schema:issueNumber 3
190 rdf:type schema:PublicationIssue
191 Nbc8456f437a541f190012d02c9b49f85 rdf:first sg:person.015663503347.24
192 rdf:rest rdf:nil
193 Nc1247f2804ec41b3bdf3406ee2c83d3f schema:name pubmed_id
194 schema:value 16177820
195 rdf:type schema:PropertyValue
196 Ncdbb9a9fb6ec42448f8b92e22bbf3175 rdf:first sg:person.01261450260.05
197 rdf:rest N64edd06bf23547c0b8081de94ae00530
198 Nce2e6cff60d84da38fa490b5707c8e07 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
199 schema:name Herpesvirus 4, Human
200 rdf:type schema:DefinedTerm
201 Nd172b79d254e4514a67f5c900f602915 schema:name doi
202 schema:value 10.1038/sj.gt.3302644
203 rdf:type schema:PropertyValue
204 Nd329c55507ac44d7b43552ff4e6e2fea rdf:first sg:person.01056176550.22
205 rdf:rest N42d13e99a94d4b70b10ac628502905ae
206 Nd8eebb8605ba4770a4dec8b9e4aacd19 schema:name Springer Nature - SN SciGraph project
207 rdf:type schema:Organization
208 Neb61120614ce4a8e9b082a16c69a2328 rdf:first sg:person.01036122220.21
209 rdf:rest N7d0cef4164ec452e979d7f5c81b637c7
210 Nf11e473414cc4d9c92f87b93272c42cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
211 schema:name Animals
212 rdf:type schema:DefinedTerm
213 Nf18df6dff93b47d190a8ac558bd5ed74 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
214 schema:name Genetic Therapy
215 rdf:type schema:DefinedTerm
216 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
217 schema:name Medical and Health Sciences
218 rdf:type schema:DefinedTerm
219 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
220 schema:name Clinical Sciences
221 rdf:type schema:DefinedTerm
222 sg:journal.1105638 schema:issn 0969-7128
223 1476-5462
224 schema:name Gene Therapy
225 schema:publisher Springer Nature
226 rdf:type schema:Periodical
227 sg:person.01036122220.21 schema:affiliation grid-institutes:grid.267370.7
228 schema:familyName Lee
229 schema:givenName K-U
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036122220.21
231 rdf:type schema:Person
232 sg:person.01056176550.22 schema:affiliation grid-institutes:grid.412091.f
233 schema:familyName Kim
234 schema:givenName Y D
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056176550.22
236 rdf:type schema:Person
237 sg:person.01163711676.37 schema:affiliation grid-institutes:grid.15444.30
238 schema:familyName Lee
239 schema:givenName H C
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163711676.37
241 rdf:type schema:Person
242 sg:person.01175446071.86 schema:affiliation grid-institutes:grid.412091.f
243 schema:familyName Kim
244 schema:givenName S-Y
245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175446071.86
246 rdf:type schema:Person
247 sg:person.01241111761.53 schema:affiliation grid-institutes:grid.136593.b
248 schema:familyName Kaneda
249 schema:givenName Y
250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241111761.53
251 rdf:type schema:Person
252 sg:person.01261450260.05 schema:affiliation grid-institutes:grid.412091.f
253 schema:familyName Kim
254 schema:givenName H-S
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261450260.05
256 rdf:type schema:Person
257 sg:person.01307574076.07 schema:affiliation grid-institutes:grid.412091.f
258 schema:familyName Song
259 schema:givenName D-K
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307574076.07
261 rdf:type schema:Person
262 sg:person.01313752601.46 schema:affiliation grid-institutes:grid.412091.f
263 schema:familyName Nam
264 schema:givenName C-W
265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313752601.46
266 rdf:type schema:Person
267 sg:person.01334645231.38 schema:affiliation grid-institutes:grid.136593.b
268 schema:familyName Morishita
269 schema:givenName R
270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334645231.38
271 rdf:type schema:Person
272 sg:person.01354732600.15 schema:affiliation grid-institutes:grid.258803.4
273 schema:familyName Kim
274 schema:givenName B-W
275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354732600.15
276 rdf:type schema:Person
277 sg:person.014104715634.26 schema:affiliation grid-institutes:grid.267370.7
278 schema:familyName Park
279 schema:givenName J-Y
280 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014104715634.26
281 rdf:type schema:Person
282 sg:person.015663503347.24 schema:affiliation grid-institutes:grid.258803.4
283 schema:familyName Lee
284 schema:givenName I-K
285 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015663503347.24
286 rdf:type schema:Person
287 sg:person.0624115635.98 schema:affiliation grid-institutes:grid.258803.4
288 schema:familyName Kim
289 schema:givenName J-G
290 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624115635.98
291 rdf:type schema:Person
292 sg:person.0672231035.77 schema:affiliation grid-institutes:grid.412091.f
293 schema:familyName Park
294 schema:givenName K-G
295 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672231035.77
296 rdf:type schema:Person
297 sg:pub.10.1038/313812a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021158149
298 https://doi.org/10.1038/313812a0
299 rdf:type schema:CreativeWork
300 sg:pub.10.1038/35044106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021678417
301 https://doi.org/10.1038/35044106
302 rdf:type schema:CreativeWork
303 sg:pub.10.1038/357455a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044352249
304 https://doi.org/10.1038/357455a0
305 rdf:type schema:CreativeWork
306 sg:pub.10.1038/83324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047292282
307 https://doi.org/10.1038/83324
308 rdf:type schema:CreativeWork
309 sg:pub.10.1038/sj.gt.3300711 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031761115
310 https://doi.org/10.1038/sj.gt.3300711
311 rdf:type schema:CreativeWork
312 sg:pub.10.1038/sj.gt.3301849 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048568841
313 https://doi.org/10.1038/sj.gt.3301849
314 rdf:type schema:CreativeWork
315 sg:pub.10.1038/sj.gt.3302236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027361283
316 https://doi.org/10.1038/sj.gt.3302236
317 rdf:type schema:CreativeWork
318 sg:pub.10.1038/sj.gt.3302412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013480844
319 https://doi.org/10.1038/sj.gt.3302412
320 rdf:type schema:CreativeWork
321 grid-institutes:grid.136593.b schema:alternateName Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan
322 schema:name Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan
323 rdf:type schema:Organization
324 grid-institutes:grid.15444.30 schema:alternateName Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea
325 schema:name Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea
326 rdf:type schema:Organization
327 grid-institutes:grid.258803.4 schema:alternateName Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea
328 schema:name Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea
329 rdf:type schema:Organization
330 grid-institutes:grid.267370.7 schema:alternateName Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, South Korea
331 schema:name Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, South Korea
332 rdf:type schema:Organization
333 grid-institutes:grid.412091.f schema:alternateName Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea
334 Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea
335 schema:name Department of Internal Medicine & Institute for Medical Sciences, Keimyung University School of Medicine, Daegu, South Korea
336 Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea
337 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...