A LacZ-based transgenic mouse for detection of somatic gene repair events in vivo View Full Text


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Article Info

DATE

2004-09

AUTHORS

H D Nickerson, W H Colledge

ABSTRACT

Somatic gene repair of disease-causing chromosomal mutations is a novel approach for gene therapy. This method would ensure that the corrected gene is regulated by its endogenous promoter and expressed at physiological levels in the appropriate cell types. A reporter mouse, Gtrosa26(tm1Col), was generated by targeting a mutated LacZ gene to the Rosa26 locus in mouse embryonic stem (ES) cells. The LacZ gene contains a G to A point mutation, resulting in a Glu to Lys amino-acid substitution at position 461, which abrogates enzymatic activity. The gene is expressed in ES cells, primary embryonic fibroblasts, and in all tissues examined in the adult mouse, including the lung, liver, kidney, spleen, heart, brain and smooth muscle. This transgenic mouse will allow testing of gene repair strategies in vivo and identification of which cell types can be successfully targeted by chromosomal gene repair. Although low levels of gene repair were achieved in the ES cells used to generate the Gtrosa26(tm1Col) mouse, preliminary attempts at gene repair in vivo were unsuccessful, thus highlighting the difficulties that will have to be overcome to get this approach to work. More... »

PAGES

1351

References to SciGraph publications

  • 2003-01. Oligonucleotide-directed single-base DNA alterations in mouse embryonic stem cells in GENE THERAPY
  • 1998-03. In vivo site-directed mutagenesis of the factor IX gene by chimeric RNA/DNA oligonucleotides in NATURE MEDICINE
  • 1999-12. A sequence-specific gene correction by an RNA-DNA oligonucleotide in mammalian cells characterized by transfection and nuclear extract using a lacZ shuttle system in GENE THERAPY
  • 1998-09. Inflammatory responses following direct injection of plasmid DNA into skeletal muscle in GENE THERAPY
  • 2001-06. Expression of ΔF508 CFTR in normal mouse lung after site-specific modification of CFTR sequences by SFHR in GENE THERAPY
  • 2003-10. Correction/mutation of acid α-D-glucosidase gene by modified single-stranded oligonucleotides: in vitro and in vivo studies in GENE THERAPY
  • 1997-11. Targeted gene conversion in a mammalian CD34+-enriched cell population using a chimeric RNA/DNA oligonucleotide in JOURNAL OF MOLECULAR MEDICINE
  • 2001-10. Steroid hormone enhancement of gene delivery to a human airway epithelial cell line in vitro and mouse airways in vivo in GENE THERAPY
  • 2001-03. Targeted gene correction by small single-stranded oligonucleotides in mammalian cells in GENE THERAPY
  • 2001-11. An Flp indicator mouse expressing alkaline phosphatase from the ROSA26 locus in NATURE GENETICS
  • 2000-01. Localized in vivo genotypic and phenotypic correction of the albino mutation in skin by RNA-DNA oligonucleotide in NATURE BIOTECHNOLOGY
  • 2002-12. Simultaneous targeted alteration of the tyrosinase and c-kit genes by single-stranded oligonucleotides in GENE THERAPY
  • 1998-12. Stable and inheritable changes in genotype and phenotype of albino melanocytes induced by an RNA-DNA oligonucleotide in NATURE BIOTECHNOLOGY
  • 2003-09. A comparison of gene repair strategies in cell culture using a lacZ reporter system in GENE THERAPY
  • 2000-06. In vivo targeted repair of a point mutation in the canine dystrophin gene by a chimeric RNA/DNA oligonucleotide in NATURE BIOTECHNOLOGY
  • 2002-06. Optimising gene repair strategies in cell culture in GENE THERAPY
  • 1999-01. Generalized lacZ expression with the ROSA26 Cre reporter strain in NATURE GENETICS
  • 2002-06. Application of SFHR to gene therapy of monogenic disorders in GENE THERAPY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.gt.3302311

    DOI

    http://dx.doi.org/10.1038/sj.gt.3302311

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020749101

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15229632


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