Expression of human coagulation factor VIII in adipocytes transduced with the simian immunodeficiency virus agmTYO1-based vector for hemophilia A gene ... View Full Text


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Article Info

DATE

2004-01-22

AUTHORS

K Ogata, J Mimuro, J Kikuchi, T Tabata, Y Ueda, M Naito, S Madoiwa, K Takano, M Hasegawa, K Ozawa, Y Sakata

ABSTRACT

We demonstrate that transduction of adipocytes with a simian immunodeficiency virus agm TYO1 (SIVagm)-based lentiviral vector carrying the human coagulation factor VIII gene (SIVhFVIII) resulted in expression of the human FVIII transgene in vitro and in db/db mice in vivo. Cultured human adipocytes were transduced with the SIVagm vector carrying the GFP gene in a dose-dependent manner and transduction of adipocytes with SIVhFVIII resulted in efficient expression of human coagulation factor VIII (hFVIII; 320±39.8 ng/106 adipocytes/24 h) in vitro. Based upon successful transduction of adipocytes by SIV vectors carrying the lacZ gene in vivo in mice, the adipose tissue of db/db mice was transduced with SIVhFVIII. There was a transient appearance of human FVIII in mouse plasma (maximum 1.8 ng/ml) on day 11 after the injection. Transcripts of human FVIII transgene and human FVIII antigen also were detected in the adipose tissue by RT-PCR and immunofluorescence, respectively, on day 14. Emergence of anti-human FVIII antibodies 14 days after the injection of SIVhFVIII may explain the disappearance of human FVIII from the circulation. These results suggest that transduction of the adipocytes with vectors carrying the human FVIII gene may be potentially applicable for gene therapy of hemophilia A. More... »

PAGES

253-259

References to SciGraph publications

  • 1998-12-17. Adipose tissue as an endocrine and paracrine organ in INTERNATIONAL JOURNAL OF OBESITY
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1038/sj.gt.3302174

    DOI

    http://dx.doi.org/10.1038/sj.gt.3302174

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002841008

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/14737084


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