‘Advanced’ generation lentiviruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-04

AUTHORS

D Bonci, A Cittadini, M V G Latronico, U Borello, J K Aycock, A Drusco, A Innocenzi, A Follenzi, M Lavitrano, M G Monti, J Ross, L Naldini, C Peschle, G Cossu, G Condorelli

ABSTRACT

Efficient gene transduction in cardiomyocytes is a task that can be accomplished only by viral vectors. Up to now, the most commonly used vectors for this purpose have been adenoviral-derived ones. Recently, it has been demonstrated that lentiviral vectors can transduce growth-arrested cells, such as hematopoietic stem cells. Moreover, a modified form of lentiviral vector (the 'advanced' generation), containing an mRNA-stabilizer sequence and a nuclear import sequence, has been shown to significantly improve gene transduction in growth-arrested cells as compared to the third-generation vector. Therefore, we tested whether the 'advanced' generation lentivirus is capable of infecting and transducing cardiomyocytes both in vitro and in vivo, comparing efficacy in vitro against the third-generation of the same vector. Here we report that 'advanced' generation lentiviral vectors infected most (>80%) cardiomyocytes in culture, as demonstrated by immunofluorescence and FACS analyses: in contrast the percentage of cardiomyocytes infected by third-generation lentivirus was three- to four-fold lower. Moreover, 'advanced' generation lentivirus was also capable of infecting and inducing stable gene expression in adult myocardium in vivo. Thus, 'advanced' generation lentiviral vectors can be used for both in vitro and in vivo gene expression studies in the cardiomyocyte. More... »

PAGES

3301936

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3301936

DOI

http://dx.doi.org/10.1038/sj.gt.3301936

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018676645

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12692591


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cardiovascular Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lentivirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luminescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myocytes, Cardiac", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Laboratory of Hematology-Oncology, Istituto Superiore di Sanit\u00e0, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bonci", 
        "givenName": "D", 
        "id": "sg:person.010120360747.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010120360747.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Naples Federico II", 
          "id": "https://www.grid.ac/institutes/grid.4691.a", 
          "name": [
            "Department of Clinical Medicine and Cardiovascular Science, University \u2018Federico II\u2019, Naples, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cittadini", 
        "givenName": "A", 
        "id": "sg:person.0742661632.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742661632.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Thomas Jefferson University", 
          "id": "https://www.grid.ac/institutes/grid.265008.9", 
          "name": [
            "Laboratory of Hematology-Oncology, Istituto Superiore di Sanit\u00e0, Rome, Italy", 
            "Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Latronico", 
        "givenName": "M V G", 
        "id": "sg:person.0576137377.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576137377.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapienza University of Rome", 
          "id": "https://www.grid.ac/institutes/grid.7841.a", 
          "name": [
            "Stem Cell Research Institute, H. S. Raffaele, Milan, Italy", 
            "Department of Histology and Medical Embryology, University La Sapienza, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Borello", 
        "givenName": "U", 
        "id": "sg:person.0662440445.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662440445.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Laboratory of Hematology-Oncology, Istituto Superiore di Sanit\u00e0, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aycock", 
        "givenName": "J K", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Thomas Jefferson University", 
          "id": "https://www.grid.ac/institutes/grid.265008.9", 
          "name": [
            "Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Drusco", 
        "givenName": "A", 
        "id": "sg:person.01222462702.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222462702.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Stem Cell Research Institute, H. S. Raffaele, Milan, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Innocenzi", 
        "givenName": "A", 
        "id": "sg:person.01256015462.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256015462.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Gene Transfer and Therapy and Tumor Immunology, IRCCS, Institute for Cancer Research and Treatment, University of Turin Medical School, Candiolo (TO), Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Follenzi", 
        "givenName": "A", 
        "id": "sg:person.01233316110.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233316110.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Milano-Bicocca", 
          "id": "https://www.grid.ac/institutes/grid.7563.7", 
          "name": [
            "Department of Environmental and Experimental Medicine and Medical Biotechnologies, University of Milano-Bicocca, Monza (MI), Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lavitrano", 
        "givenName": "M", 
        "id": "sg:person.01267107540.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267107540.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Naples Federico II", 
          "id": "https://www.grid.ac/institutes/grid.4691.a", 
          "name": [
            "Department of Clinical Medicine and Cardiovascular Science, University \u2018Federico II\u2019, Naples, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Monti", 
        "givenName": "M G", 
        "id": "sg:person.011576104140.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011576104140.68"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Ross", 
        "givenName": "J", 
        "id": "sg:person.01235625675.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235625675.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Gene Transfer and Therapy and Tumor Immunology, IRCCS, Institute for Cancer Research and Treatment, University of Turin Medical School, Candiolo (TO), Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Naldini", 
        "givenName": "L", 
        "id": "sg:person.01200504164.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200504164.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Laboratory of Hematology-Oncology, Istituto Superiore di Sanit\u00e0, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peschle", 
        "givenName": "C", 
        "id": "sg:person.056721310.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.056721310.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapienza University of Rome", 
          "id": "https://www.grid.ac/institutes/grid.7841.a", 
          "name": [
            "Stem Cell Research Institute, H. S. Raffaele, Milan, Italy", 
            "Department of Histology and Medical Embryology, University La Sapienza, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cossu", 
        "givenName": "G", 
        "id": "sg:person.01153740373.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153740373.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapienza University of Rome", 
          "id": "https://www.grid.ac/institutes/grid.7841.a", 
          "name": [
            "Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA", 
            "II Medical School, IRCCS Neuromed, University \u2018La Sapienza\u2019, 00161 Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Condorelli", 
        "givenName": "G", 
        "id": "sg:person.016367365420.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016367365420.10"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80828-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001513555"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.191217898", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004626788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmcc.1999.1035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008913759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/76095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021315149", 
          "https://doi.org/10.1038/76095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/76095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021315149", 
          "https://doi.org/10.1038/76095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/hc0402.102953", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029042395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.9.5251", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029924784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.21.11382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031334968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-4644(20000615)77:4<529::aid-jcb2>3.0.co;2-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039052563"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.86.6.616", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045378695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/83324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292282", 
          "https://doi.org/10.1038/83324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/83324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047292282", 
          "https://doi.org/10.1038/83324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.cir.99.2.201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063338662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079607947", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083326856", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-04", 
    "datePublishedReg": "2003-04-01", 
    "description": "Efficient gene transduction in cardiomyocytes is a task that can be accomplished only by viral vectors. Up to now, the most commonly used vectors for this purpose have been adenoviral-derived ones. Recently, it has been demonstrated that lentiviral vectors can transduce growth-arrested cells, such as hematopoietic stem cells. Moreover, a modified form of lentiviral vector (the 'advanced' generation), containing an mRNA-stabilizer sequence and a nuclear import sequence, has been shown to significantly improve gene transduction in growth-arrested cells as compared to the third-generation vector. Therefore, we tested whether the 'advanced' generation lentivirus is capable of infecting and transducing cardiomyocytes both in vitro and in vivo, comparing efficacy in vitro against the third-generation of the same vector. Here we report that 'advanced' generation lentiviral vectors infected most (>80%) cardiomyocytes in culture, as demonstrated by immunofluorescence and FACS analyses: in contrast the percentage of cardiomyocytes infected by third-generation lentivirus was three- to four-fold lower. Moreover, 'advanced' generation lentivirus was also capable of infecting and inducing stable gene expression in adult myocardium in vivo. Thus, 'advanced' generation lentiviral vectors can be used for both in vitro and in vivo gene expression studies in the cardiomyocyte.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.gt.3301936", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5150099", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5148242", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "8", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "\u2018Advanced\u2019 generation lentiviruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo", 
    "pagination": "3301936", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "58fc8a15e238e26262455f50227c0eead4cd757c7a82e53a035312dfa5cd2f18"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12692591"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3301936"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1018676645"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3301936", 
      "https://app.dimensions.ai/details/publication/pub.1018676645"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_54015_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/3301936"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301936'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301936'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301936'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301936'


 

This table displays all metadata directly associated to this object as RDF triples.

281 TRIPLES      21 PREDICATES      55 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3301936 schema:about N0438f239045940a6b74eef7797f6bdf6
2 N094b8d4ebe7f48b8ac1277d49fe42f67
3 N173bb2c3429f4d2aaa071c089bb248ab
4 N4814c45645124b56bf1f1488232d5015
5 N55426c6d1113420cb70d6d69b938143d
6 N718496f3978c4b1d99310efaf47d814b
7 N7ce1c8dd921a4313bb059bd5e68b92a5
8 N83a4f899d6154da7bbab5d85317df144
9 Nb26c4ffdf1c94f15a6da97fc5831335d
10 Nb90b5761d15d411b94075d655a098f56
11 Ndfac12ebb6b34397aebcb62ea962d807
12 Nf15ab636f9fe4e78a814a87c196581a6
13 Nf498406cee2849a9bda2c316c9a11159
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author Nf364f0866ba642409533355f8c2fdd49
17 schema:citation sg:pub.10.1038/76095
18 sg:pub.10.1038/83324
19 https://app.dimensions.ai/details/publication/pub.1079607947
20 https://app.dimensions.ai/details/publication/pub.1083326856
21 https://doi.org/10.1002/(sici)1097-4644(20000615)77:4<529::aid-jcb2>3.0.co;2-x
22 https://doi.org/10.1006/jmcc.1999.1035
23 https://doi.org/10.1016/s0092-8674(00)80828-4
24 https://doi.org/10.1073/pnas.191217898
25 https://doi.org/10.1073/pnas.93.21.11382
26 https://doi.org/10.1073/pnas.95.9.5251
27 https://doi.org/10.1161/01.cir.99.2.201
28 https://doi.org/10.1161/01.res.86.6.616
29 https://doi.org/10.1161/hc0402.102953
30 schema:datePublished 2003-04
31 schema:datePublishedReg 2003-04-01
32 schema:description Efficient gene transduction in cardiomyocytes is a task that can be accomplished only by viral vectors. Up to now, the most commonly used vectors for this purpose have been adenoviral-derived ones. Recently, it has been demonstrated that lentiviral vectors can transduce growth-arrested cells, such as hematopoietic stem cells. Moreover, a modified form of lentiviral vector (the 'advanced' generation), containing an mRNA-stabilizer sequence and a nuclear import sequence, has been shown to significantly improve gene transduction in growth-arrested cells as compared to the third-generation vector. Therefore, we tested whether the 'advanced' generation lentivirus is capable of infecting and transducing cardiomyocytes both in vitro and in vivo, comparing efficacy in vitro against the third-generation of the same vector. Here we report that 'advanced' generation lentiviral vectors infected most (>80%) cardiomyocytes in culture, as demonstrated by immunofluorescence and FACS analyses: in contrast the percentage of cardiomyocytes infected by third-generation lentivirus was three- to four-fold lower. Moreover, 'advanced' generation lentivirus was also capable of infecting and inducing stable gene expression in adult myocardium in vivo. Thus, 'advanced' generation lentiviral vectors can be used for both in vitro and in vivo gene expression studies in the cardiomyocyte.
33 schema:genre research_article
34 schema:inLanguage en
35 schema:isAccessibleForFree true
36 schema:isPartOf Ncbd3b72a06a342b48efba13106b9bbd8
37 Nf8d31bd649aa49ad9d3c66144693fd64
38 sg:journal.1105638
39 schema:name ‘Advanced’ generation lentiviruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo
40 schema:pagination 3301936
41 schema:productId N4faacae69fdb4233b84b1afbc31fc578
42 N76ad20bb49f14a3abef4f95b2a797df1
43 Nacad38c0cd1f4ffc89a297fe82de6bde
44 Nd216d3a5c1224ebda3a05a4ba377b29f
45 Ndbffb58366c048008ed5f8d9025ffc2b
46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018676645
47 https://doi.org/10.1038/sj.gt.3301936
48 schema:sdDatePublished 2019-04-11T12:15
49 schema:sdLicense https://scigraph.springernature.com/explorer/license/
50 schema:sdPublisher N674df5a565d14ed1978a98da8e1e4d0e
51 schema:url http://www.nature.com/articles/3301936
52 sgo:license sg:explorer/license/
53 sgo:sdDataset articles
54 rdf:type schema:ScholarlyArticle
55 N0438f239045940a6b74eef7797f6bdf6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Cardiovascular Diseases
57 rdf:type schema:DefinedTerm
58 N094b8d4ebe7f48b8ac1277d49fe42f67 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
59 schema:name Lentivirus
60 rdf:type schema:DefinedTerm
61 N0ed41881090040fa85ac14c3573561ef rdf:first N99dd675f185f44129b07978828801da9
62 rdf:rest Nf1dde781860846cb81ed3d871582b02f
63 N11bc67854b7a4159972a89ecbaf9c393 rdf:first sg:person.011576104140.68
64 rdf:rest Ne71860b6400046889fd15ab6656a4b55
65 N173bb2c3429f4d2aaa071c089bb248ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Genetic Vectors
67 rdf:type schema:DefinedTerm
68 N1ea3dd5c2d7f4467a26fda97d9ba5235 rdf:first sg:person.016367365420.10
69 rdf:rest rdf:nil
70 N2ab2d8f41171477aba4ef7343d75fcc2 rdf:first sg:person.01233316110.24
71 rdf:rest N518a7fbc9e624212b138088dbf26adc4
72 N336c200e4d2e4e88bb9b461d258079f8 rdf:first sg:person.056721310.67
73 rdf:rest N490fe70a01aa4eeca25602c9ba0afcb6
74 N4814c45645124b56bf1f1488232d5015 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Cell Line
76 rdf:type schema:DefinedTerm
77 N490fe70a01aa4eeca25602c9ba0afcb6 rdf:first sg:person.01153740373.28
78 rdf:rest N1ea3dd5c2d7f4467a26fda97d9ba5235
79 N4faacae69fdb4233b84b1afbc31fc578 schema:name doi
80 schema:value 10.1038/sj.gt.3301936
81 rdf:type schema:PropertyValue
82 N518a7fbc9e624212b138088dbf26adc4 rdf:first sg:person.01267107540.93
83 rdf:rest N11bc67854b7a4159972a89ecbaf9c393
84 N55426c6d1113420cb70d6d69b938143d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Rats
86 rdf:type schema:DefinedTerm
87 N674df5a565d14ed1978a98da8e1e4d0e schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 N718496f3978c4b1d99310efaf47d814b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Animals
91 rdf:type schema:DefinedTerm
92 N720a53a117904a7b980c1b3bc710d136 rdf:first sg:person.01256015462.02
93 rdf:rest N2ab2d8f41171477aba4ef7343d75fcc2
94 N76ad20bb49f14a3abef4f95b2a797df1 schema:name readcube_id
95 schema:value 58fc8a15e238e26262455f50227c0eead4cd757c7a82e53a035312dfa5cd2f18
96 rdf:type schema:PropertyValue
97 N7ce1c8dd921a4313bb059bd5e68b92a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Flow Cytometry
99 rdf:type schema:DefinedTerm
100 N83a4f899d6154da7bbab5d85317df144 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Genetic Therapy
102 rdf:type schema:DefinedTerm
103 N92f125bfba5444d2850d1c9f716b8c8a rdf:first sg:person.01200504164.28
104 rdf:rest N336c200e4d2e4e88bb9b461d258079f8
105 N99dd675f185f44129b07978828801da9 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
106 schema:familyName Aycock
107 schema:givenName J K
108 rdf:type schema:Person
109 Na91a7167c3f64ece9c8521c46aaf1e19 rdf:first sg:person.0576137377.72
110 rdf:rest Nab4428bc212d43e392412068a7e40616
111 Nab4428bc212d43e392412068a7e40616 rdf:first sg:person.0662440445.66
112 rdf:rest N0ed41881090040fa85ac14c3573561ef
113 Nacad38c0cd1f4ffc89a297fe82de6bde schema:name dimensions_id
114 schema:value pub.1018676645
115 rdf:type schema:PropertyValue
116 Nb26c4ffdf1c94f15a6da97fc5831335d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Transduction, Genetic
118 rdf:type schema:DefinedTerm
119 Nb90b5761d15d411b94075d655a098f56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Myocytes, Cardiac
121 rdf:type schema:DefinedTerm
122 Ncbd3b72a06a342b48efba13106b9bbd8 schema:volumeNumber 10
123 rdf:type schema:PublicationVolume
124 Nd216d3a5c1224ebda3a05a4ba377b29f schema:name pubmed_id
125 schema:value 12692591
126 rdf:type schema:PropertyValue
127 Ndbffb58366c048008ed5f8d9025ffc2b schema:name nlm_unique_id
128 schema:value 9421525
129 rdf:type schema:PropertyValue
130 Ndfac12ebb6b34397aebcb62ea962d807 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Luminescent Proteins
132 rdf:type schema:DefinedTerm
133 Ne71860b6400046889fd15ab6656a4b55 rdf:first sg:person.01235625675.10
134 rdf:rest N92f125bfba5444d2850d1c9f716b8c8a
135 Nea1662850cbe4c45b1c9bff490de9e8a schema:name Stem Cell Research Institute, H. S. Raffaele, Milan, Italy
136 rdf:type schema:Organization
137 Nf15ab636f9fe4e78a814a87c196581a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Green Fluorescent Proteins
139 rdf:type schema:DefinedTerm
140 Nf1dde781860846cb81ed3d871582b02f rdf:first sg:person.01222462702.64
141 rdf:rest N720a53a117904a7b980c1b3bc710d136
142 Nf364f0866ba642409533355f8c2fdd49 rdf:first sg:person.010120360747.55
143 rdf:rest Nf51bd8dd8c5f418792ea5e2721a263dc
144 Nf498406cee2849a9bda2c316c9a11159 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Microscopy, Fluorescence
146 rdf:type schema:DefinedTerm
147 Nf51bd8dd8c5f418792ea5e2721a263dc rdf:first sg:person.0742661632.97
148 rdf:rest Na91a7167c3f64ece9c8521c46aaf1e19
149 Nf8d31bd649aa49ad9d3c66144693fd64 schema:issueNumber 8
150 rdf:type schema:PublicationIssue
151 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
152 schema:name Biological Sciences
153 rdf:type schema:DefinedTerm
154 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
155 schema:name Genetics
156 rdf:type schema:DefinedTerm
157 sg:grant.5148242 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3301936
158 rdf:type schema:MonetaryGrant
159 sg:grant.5150099 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3301936
160 rdf:type schema:MonetaryGrant
161 sg:journal.1105638 schema:issn 0969-7128
162 1476-5462
163 schema:name Gene Therapy
164 rdf:type schema:Periodical
165 sg:person.010120360747.55 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
166 schema:familyName Bonci
167 schema:givenName D
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010120360747.55
169 rdf:type schema:Person
170 sg:person.01153740373.28 schema:affiliation https://www.grid.ac/institutes/grid.7841.a
171 schema:familyName Cossu
172 schema:givenName G
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153740373.28
174 rdf:type schema:Person
175 sg:person.011576104140.68 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
176 schema:familyName Monti
177 schema:givenName M G
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011576104140.68
179 rdf:type schema:Person
180 sg:person.01200504164.28 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
181 schema:familyName Naldini
182 schema:givenName L
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200504164.28
184 rdf:type schema:Person
185 sg:person.01222462702.64 schema:affiliation https://www.grid.ac/institutes/grid.265008.9
186 schema:familyName Drusco
187 schema:givenName A
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222462702.64
189 rdf:type schema:Person
190 sg:person.01233316110.24 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
191 schema:familyName Follenzi
192 schema:givenName A
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233316110.24
194 rdf:type schema:Person
195 sg:person.01235625675.10 schema:familyName Ross
196 schema:givenName J
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235625675.10
198 rdf:type schema:Person
199 sg:person.01256015462.02 schema:affiliation Nea1662850cbe4c45b1c9bff490de9e8a
200 schema:familyName Innocenzi
201 schema:givenName A
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256015462.02
203 rdf:type schema:Person
204 sg:person.01267107540.93 schema:affiliation https://www.grid.ac/institutes/grid.7563.7
205 schema:familyName Lavitrano
206 schema:givenName M
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267107540.93
208 rdf:type schema:Person
209 sg:person.016367365420.10 schema:affiliation https://www.grid.ac/institutes/grid.7841.a
210 schema:familyName Condorelli
211 schema:givenName G
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016367365420.10
213 rdf:type schema:Person
214 sg:person.056721310.67 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
215 schema:familyName Peschle
216 schema:givenName C
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.056721310.67
218 rdf:type schema:Person
219 sg:person.0576137377.72 schema:affiliation https://www.grid.ac/institutes/grid.265008.9
220 schema:familyName Latronico
221 schema:givenName M V G
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576137377.72
223 rdf:type schema:Person
224 sg:person.0662440445.66 schema:affiliation https://www.grid.ac/institutes/grid.7841.a
225 schema:familyName Borello
226 schema:givenName U
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662440445.66
228 rdf:type schema:Person
229 sg:person.0742661632.97 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
230 schema:familyName Cittadini
231 schema:givenName A
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742661632.97
233 rdf:type schema:Person
234 sg:pub.10.1038/76095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021315149
235 https://doi.org/10.1038/76095
236 rdf:type schema:CreativeWork
237 sg:pub.10.1038/83324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047292282
238 https://doi.org/10.1038/83324
239 rdf:type schema:CreativeWork
240 https://app.dimensions.ai/details/publication/pub.1079607947 schema:CreativeWork
241 https://app.dimensions.ai/details/publication/pub.1083326856 schema:CreativeWork
242 https://doi.org/10.1002/(sici)1097-4644(20000615)77:4<529::aid-jcb2>3.0.co;2-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1039052563
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1006/jmcc.1999.1035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008913759
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s0092-8674(00)80828-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001513555
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1073/pnas.191217898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004626788
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1073/pnas.93.21.11382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031334968
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1073/pnas.95.9.5251 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029924784
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1161/01.cir.99.2.201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063338662
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1161/01.res.86.6.616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045378695
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1161/hc0402.102953 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029042395
259 rdf:type schema:CreativeWork
260 https://www.grid.ac/institutes/grid.265008.9 schema:alternateName Thomas Jefferson University
261 schema:name Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA
262 Laboratory of Hematology-Oncology, Istituto Superiore di Sanità, Rome, Italy
263 rdf:type schema:Organization
264 https://www.grid.ac/institutes/grid.416651.1 schema:alternateName Istituto Superiore di Sanità
265 schema:name Laboratory of Hematology-Oncology, Istituto Superiore di Sanità, Rome, Italy
266 rdf:type schema:Organization
267 https://www.grid.ac/institutes/grid.4691.a schema:alternateName University of Naples Federico II
268 schema:name Department of Clinical Medicine and Cardiovascular Science, University ‘Federico II’, Naples, Italy
269 rdf:type schema:Organization
270 https://www.grid.ac/institutes/grid.7563.7 schema:alternateName University of Milano-Bicocca
271 schema:name Department of Environmental and Experimental Medicine and Medical Biotechnologies, University of Milano-Bicocca, Monza (MI), Italy
272 rdf:type schema:Organization
273 https://www.grid.ac/institutes/grid.7605.4 schema:alternateName University of Turin
274 schema:name Gene Transfer and Therapy and Tumor Immunology, IRCCS, Institute for Cancer Research and Treatment, University of Turin Medical School, Candiolo (TO), Italy
275 rdf:type schema:Organization
276 https://www.grid.ac/institutes/grid.7841.a schema:alternateName Sapienza University of Rome
277 schema:name Department of Histology and Medical Embryology, University La Sapienza, Rome, Italy
278 II Medical School, IRCCS Neuromed, University ‘La Sapienza’, 00161 Rome, Italy
279 Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA
280 Stem Cell Research Institute, H. S. Raffaele, Milan, Italy
281 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...