Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2001-08

AUTHORS

DM McCarty, PE Monahan, RJ Samulski

ABSTRACT

Adeno-associated virus (AAV) vectors package single-stranded genomes and require host-cell synthesis of the complementary strand for transduction. However, when the genome is half wild-type size, AAV can package either two copies, or dimeric inverted repeat DNA molecules. Dimeric, or self-complementary molecules (scAAV) should spontaneously reanneal, alleviating the requirement for host-cell DNA synthesis. We generated and characterized scAAV vectors in order to bypass the rate-limiting step of second-strand synthesis. In vitro, scAAV vectors were five- to 140-fold more efficient transducing agents than conventional rAAV, with a 5.9:1 particle to transducing unit ratio. This efficiency is neither greatly increased by co-infection with Ad, nor inhibited by hydroxyurea, demonstrating that transduction is independent of DNA synthesis. In vivo, scAAV expressing erythropoietin resulted in rapid and higher levels of hematocrit than a conventional single-stranded vector. These novel scAAV vectors represent a biochemical intermediate in rAAV transduction and should provide new insights into the biology of vector transduction. More... »

PAGES

1248

Journal

TITLE

Gene Therapy

ISSUE

16

VOLUME

8

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.gt.3301514

    DOI

    http://dx.doi.org/10.1038/sj.gt.3301514

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024953759

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11509958


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