Degradable polymeric carrier for the delivery of IL-10 plasmid DNA to prevent autoimmune insulitis of NOD mice View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-12

AUTHORS

J J Koh, K S Ko, M Lee, S Han, J S Park, S W Kim

ABSTRACT

Recently, we have reported that biodegradable poly [alpha-(4-aminobutyl)-L-glycolic acid] (PAGA) can condense and protect plasmid DNA from DNase I. In this study, we investigated whether the systemic administration of pCAGGS mouse IL-10 (mIL-10) expression plasmid complexed with PAGA can reduce the development of insulitis in non-obese diabetic (NOD) mice. PAGA/mIL-10 plasmid complexes were stable for more than 60 min, but the naked DNA was destroyed within 10 min by DNase I. The PAGA/DNA complexes were injected into the tail vein of 3-week-old NOD mice. Serum mIL-10 level peaked at 5 days after injection, and could be detected for more than 9 weeks. The prevalence of severe insulitis on 12-week-old NOD mice was markedly reduced by the intravenous injection of PAGA/DNA complex (15.7%) compared with that of naked DNA injection (34.5%) and non-treated controls (90.9%). In conclusion, systemic administration of pCAGGS mIL-10 plasmid/PAGA complexes can reduce the severity of insulitis in NOD mice. This study shows that the PAGA/DNA complex has the potential for the prevention of autoimmune diabetes mellitus. Gene Therapy (2000) 7, 2099-2104. More... »

PAGES

3301334

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3301334

DOI

http://dx.doi.org/10.1038/sj.gt.3301334

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009117251

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11223991


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