Gene transfer into stimulated and unstimulated T lymphocytes by HIV-1-derived lentiviral vectors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-04-01

AUTHORS

E Costello, M Munoz, E Buetti, P R A Meylan, H Diggelmann, M Thali

ABSTRACT

Genetic modification of T lymphocytes holds great potential for treatments of cancer, T cell disorders and AIDS. While in the past recombinant murine retroviruses were the vectors of choice for gene delivery to T cells, vectors based on lentiviruses can provide additional benefits. Here, we show that VSV-G pseudotyped HIV 1 vector particles delivering the enhanced green fluorescent protein (EGFP) efficiently transduce human T lymphocytes. Transduction efficiency was optimal when infection included centrifugation of cells with concentrated vector supernatant in the presence of Polybrene. In contrast to previous reports describing murine retrovirus-mediated gene transfer to T lymphocytes, fibronectin did not improve the transduction efficiency of the VSVG-pseudotyped HIV-1 particles. Similar gene transfer efficiencies were observed following stimulation of cells with PHA/IL-2 or anti-CD3i/CD28i antibodies, although greater transgene expression was observed in the latter case. Interestingly, production of vectors in the absence of the accessory proteins Vif, Vpr, Vpu and Nef was accompanied by a 50% decrease in transduction efficiency in activated T cells. Transduction of T cells that were not stimulated before infection was achieved. No transduction of non-prestimulated cells was observed with a GALV-pseudotyped murine retroviral vector. The requirement for accessory proteins in non-prestimulated cells was more pronounced. Our results have implications for lentiviral vector targeting of other cells of the hematopoietic system including stem cells. More... »

PAGES

596-604

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3301135

DOI

http://dx.doi.org/10.1038/sj.gt.3301135

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037289278

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10819575


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Indicators and Reagents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luminescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphocyte Activation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Microbiology, University of Lausanne, Switzerland", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Institute of Microbiology, University of Lausanne, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Costello", 
        "givenName": "E", 
        "id": "sg:person.0667260706.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667260706.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Microbiology, University of Lausanne, Switzerland", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Institute of Microbiology, University of Lausanne, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Munoz", 
        "givenName": "M", 
        "id": "sg:person.0661004565.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661004565.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Microbiology, University of Lausanne, Switzerland", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Institute of Microbiology, University of Lausanne, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Buetti", 
        "givenName": "E", 
        "id": "sg:person.014742727005.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014742727005.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Microbiology, University of Lausanne, Switzerland", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Institute of Microbiology, University of Lausanne, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meylan", 
        "givenName": "P R A", 
        "id": "sg:person.01336350503.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336350503.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Microbiology, University of Lausanne, Switzerland", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Institute of Microbiology, University of Lausanne, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Diggelmann", 
        "givenName": "H", 
        "id": "sg:person.01127251017.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127251017.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Microbiology, University of Lausanne, Switzerland", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Institute of Microbiology, University of Lausanne, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thali", 
        "givenName": "M", 
        "id": "sg:person.0636677244.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636677244.50"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1016/s0925-5710(97)90114-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054664126", 
          "https://doi.org/10.1016/s0925-5710(97)90114-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/38410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048288589", 
          "https://doi.org/10.1038/38410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1197-314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045816864", 
          "https://doi.org/10.1038/ng1197-314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4615-1995-9_3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051225285", 
          "https://doi.org/10.1007/978-1-4615-1995-9_3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0997-871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006020704", 
          "https://doi.org/10.1038/nbt0997-871"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-04-01", 
    "datePublishedReg": "2000-04-01", 
    "description": "Genetic modification of T lymphocytes holds great potential for treatments of cancer, T cell disorders and AIDS. While in the past recombinant murine retroviruses were the vectors of choice for gene delivery to T cells, vectors based on lentiviruses can provide additional benefits. Here, we show that VSV-G pseudotyped HIV 1 vector particles delivering the enhanced green fluorescent protein (EGFP) efficiently transduce human T lymphocytes. Transduction efficiency was optimal when infection included centrifugation of cells with concentrated vector supernatant in the presence of Polybrene. In contrast to previous reports describing murine retrovirus-mediated gene transfer to T lymphocytes, fibronectin did not improve the transduction efficiency of the VSVG-pseudotyped HIV-1 particles. Similar gene transfer efficiencies were observed following stimulation of cells with PHA/IL-2 or anti-CD3i/CD28i antibodies, although greater transgene expression was observed in the latter case. Interestingly, production of vectors in the absence of the accessory proteins Vif, Vpr, Vpu and Nef was accompanied by a 50% decrease in transduction efficiency in activated T cells. Transduction of T cells that were not stimulated before infection was achieved. No transduction of non-prestimulated cells was observed with a GALV-pseudotyped murine retroviral vector. The requirement for accessory proteins in non-prestimulated cells was more pronounced. Our results have implications for lentiviral vector targeting of other cells of the hematopoietic system including stem cells.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/sj.gt.3301135", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "keywords": [
      "transduction efficiency", 
      "enhanced green fluorescent protein", 
      "production of vectors", 
      "HIV-1-derived lentiviral vectors", 
      "vector of choice", 
      "greater transgene expression", 
      "HIV-1 vector particles", 
      "similar gene transfer efficiency", 
      "gene transfer efficiency", 
      "gene transfer", 
      "gene delivery", 
      "murine retroviral vector", 
      "vector targeting", 
      "vector supernatants", 
      "recombinant murine retrovirus", 
      "transgene expression", 
      "lentiviral vectors", 
      "vector particles", 
      "presence of polybrene", 
      "genetic modification", 
      "retroviral vectors", 
      "great potential", 
      "green fluorescent protein", 
      "murine retrovirus", 
      "transfer efficiency", 
      "fluorescent protein", 
      "stem cells", 
      "efficiency", 
      "treatment of cancer", 
      "vector", 
      "particles", 
      "unstimulated T lymphocytes", 
      "VSVG", 
      "GALV", 
      "retroviruses", 
      "VSV", 
      "delivery", 
      "polybrene", 
      "targeting", 
      "transfer", 
      "lentivirus", 
      "cells", 
      "transduction", 
      "modification", 
      "requirements", 
      "PHA/IL", 
      "potential", 
      "centrifugation of cells", 
      "centrifugation", 
      "production", 
      "antibodies", 
      "system", 
      "protein", 
      "hematopoietic system", 
      "supernatant", 
      "HIV-1 particles", 
      "additional benefit", 
      "T-cell disorders", 
      "accessory proteins", 
      "accessory protein Vif", 
      "presence", 
      "results", 
      "latter case", 
      "previous reports", 
      "human T lymphocytes", 
      "benefits", 
      "contrast", 
      "fibronectin", 
      "expression", 
      "Vpr", 
      "decrease", 
      "choice", 
      "stimulation of cells", 
      "absence", 
      "treatment", 
      "infection", 
      "Vpu", 
      "cancer", 
      "report", 
      "T cells", 
      "cases", 
      "IL", 
      "T lymphocytes", 
      "Vif", 
      "Nef", 
      "cell disorders", 
      "lymphocytes", 
      "disorders", 
      "stimulation", 
      "implications", 
      "activated T cells", 
      "AIDS"
    ], 
    "name": "Gene transfer into stimulated and unstimulated T lymphocytes by HIV-1-derived lentiviral vectors", 
    "pagination": "596-604", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037289278"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3301135"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10819575"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3301135", 
      "https://app.dimensions.ai/details/publication/pub.1037289278"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:22", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_322.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/sj.gt.3301135"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301135'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301135'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301135'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3301135'


 

This table displays all metadata directly associated to this object as RDF triples.

260 TRIPLES      21 PREDICATES      135 URIs      122 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3301135 schema:about N15bb51f617ba4849858ddb448a11fa74
2 N244af8cc1fc04d82ae7faac1c294c736
3 N26748a83a02e4b51a73d085fb6f4035c
4 N5ca811d373aa4682abd2515d0aa1029a
5 N62df9ea401b94aa888036e32225d4d40
6 N707ecc615ff7499e903920ed1b65b111
7 N875e0a1e4c164a719af1fc71888e6ff5
8 N8fdf566677d64381a9f18b2bf20a4552
9 N95c6aae80db045c9bf5408e567f19d36
10 Na83a034ad3a64ce899502e827e43b95f
11 Nb4da249d896b4b928ca062cba884f34b
12 Nbc440cf03b6644288a964fdfff349552
13 Ne0a02f27fe1b4af78ab219ded6fdd403
14 anzsrc-for:06
15 anzsrc-for:11
16 schema:author N3e7904a9e3ee4e20a523e548847cbef3
17 schema:citation sg:pub.10.1007/978-1-4615-1995-9_3
18 sg:pub.10.1016/s0925-5710(97)90114-6
19 sg:pub.10.1038/38410
20 sg:pub.10.1038/nbt0997-871
21 sg:pub.10.1038/ng1197-314
22 schema:datePublished 2000-04-01
23 schema:datePublishedReg 2000-04-01
24 schema:description Genetic modification of T lymphocytes holds great potential for treatments of cancer, T cell disorders and AIDS. While in the past recombinant murine retroviruses were the vectors of choice for gene delivery to T cells, vectors based on lentiviruses can provide additional benefits. Here, we show that VSV-G pseudotyped HIV 1 vector particles delivering the enhanced green fluorescent protein (EGFP) efficiently transduce human T lymphocytes. Transduction efficiency was optimal when infection included centrifugation of cells with concentrated vector supernatant in the presence of Polybrene. In contrast to previous reports describing murine retrovirus-mediated gene transfer to T lymphocytes, fibronectin did not improve the transduction efficiency of the VSVG-pseudotyped HIV-1 particles. Similar gene transfer efficiencies were observed following stimulation of cells with PHA/IL-2 or anti-CD3i/CD28i antibodies, although greater transgene expression was observed in the latter case. Interestingly, production of vectors in the absence of the accessory proteins Vif, Vpr, Vpu and Nef was accompanied by a 50% decrease in transduction efficiency in activated T cells. Transduction of T cells that were not stimulated before infection was achieved. No transduction of non-prestimulated cells was observed with a GALV-pseudotyped murine retroviral vector. The requirement for accessory proteins in non-prestimulated cells was more pronounced. Our results have implications for lentiviral vector targeting of other cells of the hematopoietic system including stem cells.
25 schema:genre article
26 schema:isAccessibleForFree true
27 schema:isPartOf N1580ee30562d41a8b51f05dc5cb37c92
28 N692408ef5083487cbbf87124ca0be5ff
29 sg:journal.1105638
30 schema:keywords AIDS
31 GALV
32 HIV-1 particles
33 HIV-1 vector particles
34 HIV-1-derived lentiviral vectors
35 IL
36 Nef
37 PHA/IL
38 T cells
39 T lymphocytes
40 T-cell disorders
41 VSV
42 VSVG
43 Vif
44 Vpr
45 Vpu
46 absence
47 accessory protein Vif
48 accessory proteins
49 activated T cells
50 additional benefit
51 antibodies
52 benefits
53 cancer
54 cases
55 cell disorders
56 cells
57 centrifugation
58 centrifugation of cells
59 choice
60 contrast
61 decrease
62 delivery
63 disorders
64 efficiency
65 enhanced green fluorescent protein
66 expression
67 fibronectin
68 fluorescent protein
69 gene delivery
70 gene transfer
71 gene transfer efficiency
72 genetic modification
73 great potential
74 greater transgene expression
75 green fluorescent protein
76 hematopoietic system
77 human T lymphocytes
78 implications
79 infection
80 latter case
81 lentiviral vectors
82 lentivirus
83 lymphocytes
84 modification
85 murine retroviral vector
86 murine retrovirus
87 particles
88 polybrene
89 potential
90 presence
91 presence of polybrene
92 previous reports
93 production
94 production of vectors
95 protein
96 recombinant murine retrovirus
97 report
98 requirements
99 results
100 retroviral vectors
101 retroviruses
102 similar gene transfer efficiency
103 stem cells
104 stimulation
105 stimulation of cells
106 supernatant
107 system
108 targeting
109 transduction
110 transduction efficiency
111 transfer
112 transfer efficiency
113 transgene expression
114 treatment
115 treatment of cancer
116 unstimulated T lymphocytes
117 vector
118 vector of choice
119 vector particles
120 vector supernatants
121 vector targeting
122 schema:name Gene transfer into stimulated and unstimulated T lymphocytes by HIV-1-derived lentiviral vectors
123 schema:pagination 596-604
124 schema:productId N0a0b6b3a41b64d798837fc129f905909
125 Ne269f818997d4362a4ad3356fa730e71
126 Ne49db76fbda543f7bb77ecaa0fddc14f
127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037289278
128 https://doi.org/10.1038/sj.gt.3301135
129 schema:sdDatePublished 2022-12-01T06:22
130 schema:sdLicense https://scigraph.springernature.com/explorer/license/
131 schema:sdPublisher Nc20f40092b5a4af0a666084c5306ca35
132 schema:url https://doi.org/10.1038/sj.gt.3301135
133 sgo:license sg:explorer/license/
134 sgo:sdDataset articles
135 rdf:type schema:ScholarlyArticle
136 N0a0b6b3a41b64d798837fc129f905909 schema:name doi
137 schema:value 10.1038/sj.gt.3301135
138 rdf:type schema:PropertyValue
139 N1580ee30562d41a8b51f05dc5cb37c92 schema:volumeNumber 7
140 rdf:type schema:PublicationVolume
141 N15bb51f617ba4849858ddb448a11fa74 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Luminescent Proteins
143 rdf:type schema:DefinedTerm
144 N244af8cc1fc04d82ae7faac1c294c736 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Cell Line
146 rdf:type schema:DefinedTerm
147 N26748a83a02e4b51a73d085fb6f4035c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Genetic Engineering
149 rdf:type schema:DefinedTerm
150 N3671ae0caaf54a63a691510dc257d3f9 rdf:first sg:person.01336350503.09
151 rdf:rest Nd00e5dc99fc44dc3b62ecabbd9266511
152 N3e7904a9e3ee4e20a523e548847cbef3 rdf:first sg:person.0667260706.15
153 rdf:rest N468d6bbd65904287bfb774fcbf7ee945
154 N468d6bbd65904287bfb774fcbf7ee945 rdf:first sg:person.0661004565.06
155 rdf:rest Ne3d4793d8ac042c9a35acb6a34bb20ad
156 N495fccf642b44f2ca4e58456cdcf9b26 rdf:first sg:person.0636677244.50
157 rdf:rest rdf:nil
158 N5ca811d373aa4682abd2515d0aa1029a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Genetic Vectors
160 rdf:type schema:DefinedTerm
161 N62df9ea401b94aa888036e32225d4d40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Lymphocyte Activation
163 rdf:type schema:DefinedTerm
164 N692408ef5083487cbbf87124ca0be5ff schema:issueNumber 7
165 rdf:type schema:PublicationIssue
166 N707ecc615ff7499e903920ed1b65b111 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Indicators and Reagents
168 rdf:type schema:DefinedTerm
169 N875e0a1e4c164a719af1fc71888e6ff5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Humans
171 rdf:type schema:DefinedTerm
172 N8fdf566677d64381a9f18b2bf20a4552 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Green Fluorescent Proteins
174 rdf:type schema:DefinedTerm
175 N95c6aae80db045c9bf5408e567f19d36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Genetic Therapy
177 rdf:type schema:DefinedTerm
178 Na83a034ad3a64ce899502e827e43b95f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name T-Lymphocytes
180 rdf:type schema:DefinedTerm
181 Nb4da249d896b4b928ca062cba884f34b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name HIV-1
183 rdf:type schema:DefinedTerm
184 Nbc440cf03b6644288a964fdfff349552 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Transfection
186 rdf:type schema:DefinedTerm
187 Nc20f40092b5a4af0a666084c5306ca35 schema:name Springer Nature - SN SciGraph project
188 rdf:type schema:Organization
189 Nd00e5dc99fc44dc3b62ecabbd9266511 rdf:first sg:person.01127251017.00
190 rdf:rest N495fccf642b44f2ca4e58456cdcf9b26
191 Ne0a02f27fe1b4af78ab219ded6fdd403 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Gene Expression
193 rdf:type schema:DefinedTerm
194 Ne269f818997d4362a4ad3356fa730e71 schema:name pubmed_id
195 schema:value 10819575
196 rdf:type schema:PropertyValue
197 Ne3d4793d8ac042c9a35acb6a34bb20ad rdf:first sg:person.014742727005.28
198 rdf:rest N3671ae0caaf54a63a691510dc257d3f9
199 Ne49db76fbda543f7bb77ecaa0fddc14f schema:name dimensions_id
200 schema:value pub.1037289278
201 rdf:type schema:PropertyValue
202 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
203 schema:name Biological Sciences
204 rdf:type schema:DefinedTerm
205 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
206 schema:name Medical and Health Sciences
207 rdf:type schema:DefinedTerm
208 sg:journal.1105638 schema:issn 0969-7128
209 1476-5462
210 schema:name Gene Therapy
211 schema:publisher Springer Nature
212 rdf:type schema:Periodical
213 sg:person.01127251017.00 schema:affiliation grid-institutes:None
214 schema:familyName Diggelmann
215 schema:givenName H
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127251017.00
217 rdf:type schema:Person
218 sg:person.01336350503.09 schema:affiliation grid-institutes:None
219 schema:familyName Meylan
220 schema:givenName P R A
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336350503.09
222 rdf:type schema:Person
223 sg:person.014742727005.28 schema:affiliation grid-institutes:None
224 schema:familyName Buetti
225 schema:givenName E
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014742727005.28
227 rdf:type schema:Person
228 sg:person.0636677244.50 schema:affiliation grid-institutes:None
229 schema:familyName Thali
230 schema:givenName M
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636677244.50
232 rdf:type schema:Person
233 sg:person.0661004565.06 schema:affiliation grid-institutes:None
234 schema:familyName Munoz
235 schema:givenName M
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661004565.06
237 rdf:type schema:Person
238 sg:person.0667260706.15 schema:affiliation grid-institutes:None
239 schema:familyName Costello
240 schema:givenName E
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667260706.15
242 rdf:type schema:Person
243 sg:pub.10.1007/978-1-4615-1995-9_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051225285
244 https://doi.org/10.1007/978-1-4615-1995-9_3
245 rdf:type schema:CreativeWork
246 sg:pub.10.1016/s0925-5710(97)90114-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054664126
247 https://doi.org/10.1016/s0925-5710(97)90114-6
248 rdf:type schema:CreativeWork
249 sg:pub.10.1038/38410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048288589
250 https://doi.org/10.1038/38410
251 rdf:type schema:CreativeWork
252 sg:pub.10.1038/nbt0997-871 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006020704
253 https://doi.org/10.1038/nbt0997-871
254 rdf:type schema:CreativeWork
255 sg:pub.10.1038/ng1197-314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045816864
256 https://doi.org/10.1038/ng1197-314
257 rdf:type schema:CreativeWork
258 grid-institutes:None schema:alternateName Institute of Microbiology, University of Lausanne, Switzerland
259 schema:name Institute of Microbiology, University of Lausanne, Switzerland
260 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...