Effective suicide gene therapy in vivo by EBV-based plasmid vector coupled with polyamidoamine dendrimer View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-01

AUTHORS

H Maruyama-Tabata, Y Harada, T Matsumura, E Satoh, F Cui, M Iwai, M Kita, S Hibi, J Imanishi, T Sawada, O Mazda

ABSTRACT

This study demonstrates in vivo effectiveness of a nonviral vector system, Epstein-Barr virus (EBV)-based plasmid vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBV-based vector is a plasmid vector containing EBV nuclear antigen 1 (EBNA1) gene and oriP from EBV genome. HSV-1 tk gene was transferred into Ewing's sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based plasmid vector, pSES.Tk, or a conventional plasmid vector, pS.Tk. Cells transfected with pSES.Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared with those transfected with pS. Tk/dendrimer. Intratumoral injection of pSES.Tk/dendrimer but not pS. Tk/dendrimer drastically suppressed the growth of tumors which had generated from A4573 or Huh7 hepatocellular carcinoma (HCC) cells inoculated into severe combined immunodeficiency (SCID) mice. The treatment with pSES.Tk/dendrimer also resulted in significant prolongation of survival of the mice implanted with A4573. These results suggest that the EBV/polyplex system could be useful for in vivo suicide gene therapy of cancer. Gene Therapy (2000) 7, 53-60. More... »

PAGES

3301044

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3301044

DOI

http://dx.doi.org/10.1038/sj.gt.3301044

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031286308

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10680016


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