Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-07

AUTHORS

F Liu, Y K Song, D Liu

ABSTRACT

Development of methods that allow an efficient expression of exogenous genes in animals would provide tools for gene function studies, treatment of diseases and for obtaining gene products. Therefore, we have developed a hydrodynamics-based procedure for expressing transgenes in mice by systemic administration of plasmid DNA. Using cDNA of luciferase and beta-galactosidase as a reporter gene, we demonstrated that an efficient gene transfer and expression can be achieved by a rapid injection of a large volume of DNA solution into animals via the tail vein. Among the organs expressing the transgene, the liver showed the highest level of gene expression. As high as 45 microg of luciferase protein per gram of liver can be achi- eved by a single tail vein injection of 5 microg of plasmid DNA into a mouse. Histochemical analysis using beta-galactosidase gene as a reporter reveals that approximately 40percent of hepatocytes express the transgene. The time-response curve shows that the level of transgene expression in the liver reaches the peak level in approximately 8 h after injection and decreases thereafter. The peak level of gene expression can be regained by repeated injection of plasmid DNA. These results suggest that a simple, convenient and efficient method has been developed and which can be used as an effective means for studying gene function, gene regulation and molecular pathophysiology through gene transfer, as well as for expressing proteins in animals. More... »

PAGES

3300947

Journal

TITLE

Gene Therapy

ISSUE

7

VOLUME

6

Author Affiliations

Related Patents

  • Methods For The Treatment Of Renal Failure
  • Enhanced Delivery Of Naked Dna To Skin By Non-Invasive In Vivo Electroporation
  • Conserved Hbv And Hcv Sequences Useful For Gene Silencing
  • Nucleic Acid Transfer Vector For The Introduction Of Nucleic Acid Into The Dna Of A Cell
  • Devices And Processes For Distribution Of Genetic Material To Mammalian Limb
  • Process For Delivering Sirna To Cardiac Muscle Tissue
  • In Vivo Transfection In Avians
  • Humanized Il-4 And Il-4r Alpha Animals
  • Inhibitors Of Proliferation And Activation Of Signal Transducer And Activator Of Transcription (Stats)
  • Anti-Pmel17 Antibodies And Immunoconjugates
  • Anti-Pmel17 Antibodies And Immunoconjugates
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Custom-Made Meganuclease And Use Thereof
  • Internal Clamp For Surgical Procedures
  • Devices And Processes For Distribution Of Genetic Material To Mammalian Limb
  • Custom-Made Meganuclease And Use Thereof
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Methods For Treating Eosinophilic Esophagitis By Administering An Il-4r Inhibitor
  • Rna Interference Mediated Inhibition Of Gene Expression Using Short Interfering Nucleic Acids (Sina)
  • Methods And Compositions For Rnai Mediated Inhibition Of Gene Expression In Mammals
  • Compositions And Methods For Enhanced Expression Of Recombinant Polypeptides From A Single Vector Using A Peptide Cleavage Site
  • Compositions And Methods For Enhanced Expression Of Immunoglobulins From A Single Vector Using A Peptide Cleavage Site
  • Compositions And Methods For Generating Multiple Polypeptides From A Single Vector Using A Virus Derived Peptide Cleavage Site, And Uses Thereof
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid
  • Polynucleotides For Reducing Respiratory Syncytial Virus Gene Expression
  • Custom-Made Meganuclease And Use Thereof
  • Delivery Of Molecules And Complexes To Mammalian Cells In Vivo
  • Method For Identifying Within A Mammal A Dna Encoding A Physiologically Active Polypeptide
  • Conserved Hbv And Hcv Sequences Useful For Gene Silencing
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Use Of Meganucleases For Inducing Homologous Recombination Ex Vivo And In Toto In Vertebrate Somatic Tissues And Application Thereof
  • Oncovector Nucleic Acid Molecules And Methods Of Use
  • Modular Peptide Mediated Intracellular Delivery System And Uses Therefore
  • In Vivo Transfection In Avians
  • Enzymatic Synthesis Of Poly(Amine-Co-Esters) And Methods Of Use Thereof For Gene Delivery
  • Method For Gene Transfer Into The Organelles Of Cells: Direct Gene Transfer To Mitochondria
  • Fn14/Trail Fusion Proteins And Method Of Treating Cancer
  • Compositions And Methods For Enhanced Expression Of Recombinant Polypeptides From A Single Vector Using A Peptide Cleavage Site
  • Use Of Meganucleases For Inducing Homologous Recombination Ex Vivo And In Toto In Vertebrate Somatic Tissues And Application Thereof
  • Fn14/Trail Fusion Proteins
  • Rna Interference Mediated Inhibition Of Gene Expression Using Short Interfering Nucleic Acids (Sina)
  • Compositions And Methods For Treatment Of Hematological Malignancies
  • Use Of Igf-1 In The Modulation Of Treg Cell Activity And The Treatment And Prevention Of Autoimmune Disorders Or Diseases
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Medical Fluid Injector
  • Methods And Compositions For Reducing Viral Genome Amounts In A Target Cell
  • Process For Delivery Of Polynucleotides To The Prostate
  • Recombinant Plasmid And Method For Expressing Hepatitis B Viral Antigens And Virions In Vivo
  • Hyper-Active Factor Ix Vectors For Liver-Directed Gene Therapy Of Hemophilia 'B' And Methods And Use Thereof
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Intravenous Delivery Of Polynucleotides To Cells In Mammalian Limb
  • Modulation Of Pilr To Treat Immune Disorders
  • Formulations For Targeted Release Of Agents To Low Ph Tissue Environments Or Cellular Compartments And Methods Of Use Thereof
  • Gene Therapy Compositions For Preventing And/Or Treating Autoimmune Diseases
  • Compositions For Bacterial Mediated Gene Silencing And Methods Of Using Same
  • Methods And Compositions For Reducing Viral Genome Amounts In A Target Cell
  • Chemically Modified Multifunctional Short Interfering Nucleic Acid Molecules That Mediate Rna Interference
  • Bacteria Mediated Gene Silencing
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Method For De-Differentiating A Cell
  • Circular Nucleic Acid Vectors, And Methods For Making And Using The Same
  • Transposon System And Methods Of Use
  • Internal Clamp For Surgical Procedures
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Fusion Proteins Comprising Fn14 And An Inhibitory Protein
  • Inhibitors Of Ribonucleotide Reductase Subunit 2 And Uses Thereof
  • Conserved Hbv And Hcv Sequences Useful For Gene Silencing
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Humanized Il-4 And Il-4ra Animals
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Anti-Pmel17 Antibodies And Immunoconjugates
  • Custom-Made Meganuclease And Use Thereof
  • Method For Treating Cancer By Vector-Mediated Delivery Of One Or More Anti-Angiogenic Or Pro-Apoptotic Genes
  • Production Of Recombinant Proteins In Vivo And Use For Generating Antibodies
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • I-Crei Derived Single-Chain Meganuclease And Uses Thereof
  • Humanized Il-4 And Il-4rα Animals
  • Use Of Meganucleases For Inducing Homologous Recombination Ex Vivo And In Toto In Vertebrate Somatic Tissues And Application Thereof
  • Rna Interference Mediated Inhibition Of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (Sina)
  • Circular Nucleic Acid Vectors And Methods For Making And Using The Same
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.gt.3300947

    DOI

    http://dx.doi.org/10.1038/sj.gt.3300947

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030560074

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10455434


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Southern", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Therapy", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Injections, Intravenous", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Liver", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Luciferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Inbred Strains", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Time Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Pittsburgh", 
              "id": "https://www.grid.ac/institutes/grid.21925.3d", 
              "name": [
                "Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liu", 
            "givenName": "F", 
            "id": "sg:person.0615252507.11", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615252507.11"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Pittsburgh", 
              "id": "https://www.grid.ac/institutes/grid.21925.3d", 
              "name": [
                "Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Song", 
            "givenName": "Y K", 
            "id": "sg:person.016203141374.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016203141374.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Pittsburgh", 
              "id": "https://www.grid.ac/institutes/grid.21925.3d", 
              "name": [
                "Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liu", 
            "givenName": "D", 
            "id": "sg:person.0756670103.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756670103.21"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/b978-0-7506-9265-6.50021-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000943063"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-75608-5_4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002493894", 
              "https://doi.org/10.1007/978-3-642-75608-5_4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-75608-5_5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008839676", 
              "https://doi.org/10.1007/978-3-642-75608-5_5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0959-437x(93)90126-a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012445290"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(93)17090-r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015766739"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3300424", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031544602", 
              "https://doi.org/10.1038/sj.gt.3300424"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3300424", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031544602", 
              "https://doi.org/10.1038/sj.gt.3300424"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1016248701505", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031603881", 
              "https://doi.org/10.1023/a:1016248701505"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0304-4165(81)90342-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034092280"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0304-4165(81)90342-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034092280"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.ne.19.030196.001405", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035845383"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0958-1669(92)90082-t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042420186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1749-6632.1994.tb21703.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042784167"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0167-7799(97)01137-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044585682"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-75608-5_3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044683372", 
              "https://doi.org/10.1007/978-3-642-75608-5_3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/jmcc.1997.0389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046679921"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1991.2.1-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1993.4.5-643", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271261"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1994.5.12-1477", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271343"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1996.7.5-637", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271809"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1997.8.14-1659", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271920"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1997.8.15-1763", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271932"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1690918", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062500820"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.272.5259.263", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062552729"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082423969", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082478883", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082983975", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1999-07", 
        "datePublishedReg": "1999-07-01", 
        "description": "Development of methods that allow an efficient expression of exogenous genes in animals would provide tools for gene function studies, treatment of diseases and for obtaining gene products. Therefore, we have developed a hydrodynamics-based procedure for expressing transgenes in mice by systemic administration of plasmid DNA. Using cDNA of luciferase and beta-galactosidase as a reporter gene, we demonstrated that an efficient gene transfer and expression can be achieved by a rapid injection of a large volume of DNA solution into animals via the tail vein. Among the organs expressing the transgene, the liver showed the highest level of gene expression. As high as 45 microg of luciferase protein per gram of liver can be achi- eved by a single tail vein injection of 5 microg of plasmid DNA into a mouse. Histochemical analysis using beta-galactosidase gene as a reporter reveals that approximately 40percent of hepatocytes express the transgene. The time-response curve shows that the level of transgene expression in the liver reaches the peak level in approximately 8 h after injection and decreases thereafter. The peak level of gene expression can be regained by repeated injection of plasmid DNA. These results suggest that a simple, convenient and efficient method has been developed and which can be used as an effective means for studying gene function, gene regulation and molecular pathophysiology through gene transfer, as well as for expressing proteins in animals.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/sj.gt.3300947", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1105638", 
            "issn": [
              "0969-7128", 
              "1476-5462"
            ], 
            "name": "Gene Therapy", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "6"
          }
        ], 
        "name": "Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA", 
        "pagination": "3300947", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "9195bcbbecfa7ac38a9c2a9911f23f737041da3b8b859c219bab99f6192cc6d4"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10455434"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9421525"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/sj.gt.3300947"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1030560074"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/sj.gt.3300947", 
          "https://app.dimensions.ai/details/publication/pub.1030560074"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T11:54", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29204_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/3300947"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300947'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300947'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300947'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300947'


     

    This table displays all metadata directly associated to this object as RDF triples.

    208 TRIPLES      21 PREDICATES      66 URIs      33 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/sj.gt.3300947 schema:about N1bb689398f0943d5822467d530c3c11d
    2 N23369f541cdd498d8041889322701ecd
    3 N2e793b82eaee494e9f6a825953365573
    4 N2ebfe69323fd4a67ba06982ca6f9acba
    5 N4d3f896c5e1d41d8bceb92121c514ec8
    6 N4f492e59e9bb411ead1bc8da3a20437c
    7 N90cd94bd132546e29314ae49d82cfcb8
    8 N9d7d792e61674583ae5c6e1e3d5820ad
    9 N9fc353828140408691056c9f3e80927d
    10 Nbc90d9969ee0491a8cdd1ad563a94a27
    11 Nd417d5ca2dc64ecda475a47beda1a2f2
    12 Nd4ed355afef64bc2a124c5f775c0851d
    13 anzsrc-for:06
    14 anzsrc-for:0604
    15 schema:author N48df0e7afd3242f486e9293ce31057ac
    16 schema:citation sg:pub.10.1007/978-3-642-75608-5_3
    17 sg:pub.10.1007/978-3-642-75608-5_4
    18 sg:pub.10.1007/978-3-642-75608-5_5
    19 sg:pub.10.1023/a:1016248701505
    20 sg:pub.10.1038/sj.gt.3300424
    21 https://app.dimensions.ai/details/publication/pub.1082423969
    22 https://app.dimensions.ai/details/publication/pub.1082478883
    23 https://app.dimensions.ai/details/publication/pub.1082983975
    24 https://doi.org/10.1006/jmcc.1997.0389
    25 https://doi.org/10.1016/0076-6879(93)17090-r
    26 https://doi.org/10.1016/0304-4165(81)90342-1
    27 https://doi.org/10.1016/0958-1669(92)90082-t
    28 https://doi.org/10.1016/0959-437x(93)90126-a
    29 https://doi.org/10.1016/b978-0-7506-9265-6.50021-x
    30 https://doi.org/10.1016/s0167-7799(97)01137-2
    31 https://doi.org/10.1089/hum.1991.2.1-5
    32 https://doi.org/10.1089/hum.1993.4.5-643
    33 https://doi.org/10.1089/hum.1994.5.12-1477
    34 https://doi.org/10.1089/hum.1996.7.5-637
    35 https://doi.org/10.1089/hum.1997.8.14-1659
    36 https://doi.org/10.1089/hum.1997.8.15-1763
    37 https://doi.org/10.1111/j.1749-6632.1994.tb21703.x
    38 https://doi.org/10.1126/science.1690918
    39 https://doi.org/10.1126/science.272.5259.263
    40 https://doi.org/10.1146/annurev.ne.19.030196.001405
    41 schema:datePublished 1999-07
    42 schema:datePublishedReg 1999-07-01
    43 schema:description Development of methods that allow an efficient expression of exogenous genes in animals would provide tools for gene function studies, treatment of diseases and for obtaining gene products. Therefore, we have developed a hydrodynamics-based procedure for expressing transgenes in mice by systemic administration of plasmid DNA. Using cDNA of luciferase and beta-galactosidase as a reporter gene, we demonstrated that an efficient gene transfer and expression can be achieved by a rapid injection of a large volume of DNA solution into animals via the tail vein. Among the organs expressing the transgene, the liver showed the highest level of gene expression. As high as 45 microg of luciferase protein per gram of liver can be achi- eved by a single tail vein injection of 5 microg of plasmid DNA into a mouse. Histochemical analysis using beta-galactosidase gene as a reporter reveals that approximately 40percent of hepatocytes express the transgene. The time-response curve shows that the level of transgene expression in the liver reaches the peak level in approximately 8 h after injection and decreases thereafter. The peak level of gene expression can be regained by repeated injection of plasmid DNA. These results suggest that a simple, convenient and efficient method has been developed and which can be used as an effective means for studying gene function, gene regulation and molecular pathophysiology through gene transfer, as well as for expressing proteins in animals.
    44 schema:genre research_article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree true
    47 schema:isPartOf N2c3e6009703540dcaa201aab719d2eaa
    48 N868d1fcf133e454fb6df70864b5724ca
    49 sg:journal.1105638
    50 schema:name Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA
    51 schema:pagination 3300947
    52 schema:productId N1e0333044f5a4bf0afb3533bae5ffe84
    53 N5df7f994791c4b86ae20ababfcfba67b
    54 N793c5c626e5f46b5a9d4fa69a5d4a175
    55 Na0d73d7b31e54450b9e9d681341fe22d
    56 Na912a78ec49f4e5cb1e93f0c84ab208b
    57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030560074
    58 https://doi.org/10.1038/sj.gt.3300947
    59 schema:sdDatePublished 2019-04-11T11:54
    60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    61 schema:sdPublisher N87c6a3680dc6459ba68296b7e7b58f10
    62 schema:url http://www.nature.com/articles/3300947
    63 sgo:license sg:explorer/license/
    64 sgo:sdDataset articles
    65 rdf:type schema:ScholarlyArticle
    66 N1bb689398f0943d5822467d530c3c11d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    67 schema:name Blotting, Southern
    68 rdf:type schema:DefinedTerm
    69 N1e0333044f5a4bf0afb3533bae5ffe84 schema:name doi
    70 schema:value 10.1038/sj.gt.3300947
    71 rdf:type schema:PropertyValue
    72 N23369f541cdd498d8041889322701ecd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    73 schema:name Liver
    74 rdf:type schema:DefinedTerm
    75 N2c3e6009703540dcaa201aab719d2eaa schema:issueNumber 7
    76 rdf:type schema:PublicationIssue
    77 N2e793b82eaee494e9f6a825953365573 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Luciferases
    79 rdf:type schema:DefinedTerm
    80 N2ebfe69323fd4a67ba06982ca6f9acba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Transfection
    82 rdf:type schema:DefinedTerm
    83 N48df0e7afd3242f486e9293ce31057ac rdf:first sg:person.0615252507.11
    84 rdf:rest N88681d94279341c3958ab2cd7134776b
    85 N4d3f896c5e1d41d8bceb92121c514ec8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Animals
    87 rdf:type schema:DefinedTerm
    88 N4f492e59e9bb411ead1bc8da3a20437c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Gene Expression
    90 rdf:type schema:DefinedTerm
    91 N5df7f994791c4b86ae20ababfcfba67b schema:name readcube_id
    92 schema:value 9195bcbbecfa7ac38a9c2a9911f23f737041da3b8b859c219bab99f6192cc6d4
    93 rdf:type schema:PropertyValue
    94 N793c5c626e5f46b5a9d4fa69a5d4a175 schema:name dimensions_id
    95 schema:value pub.1030560074
    96 rdf:type schema:PropertyValue
    97 N868d1fcf133e454fb6df70864b5724ca schema:volumeNumber 6
    98 rdf:type schema:PublicationVolume
    99 N87c6a3680dc6459ba68296b7e7b58f10 schema:name Springer Nature - SN SciGraph project
    100 rdf:type schema:Organization
    101 N88681d94279341c3958ab2cd7134776b rdf:first sg:person.016203141374.94
    102 rdf:rest Nd44a5cfa9a0a46d2bfad37fb7c62f22f
    103 N90cd94bd132546e29314ae49d82cfcb8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Mice, Inbred Strains
    105 rdf:type schema:DefinedTerm
    106 N9d7d792e61674583ae5c6e1e3d5820ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Mice
    108 rdf:type schema:DefinedTerm
    109 N9fc353828140408691056c9f3e80927d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Injections, Intravenous
    111 rdf:type schema:DefinedTerm
    112 Na0d73d7b31e54450b9e9d681341fe22d schema:name nlm_unique_id
    113 schema:value 9421525
    114 rdf:type schema:PropertyValue
    115 Na912a78ec49f4e5cb1e93f0c84ab208b schema:name pubmed_id
    116 schema:value 10455434
    117 rdf:type schema:PropertyValue
    118 Nbc90d9969ee0491a8cdd1ad563a94a27 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Time Factors
    120 rdf:type schema:DefinedTerm
    121 Nd417d5ca2dc64ecda475a47beda1a2f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Genetic Therapy
    123 rdf:type schema:DefinedTerm
    124 Nd44a5cfa9a0a46d2bfad37fb7c62f22f rdf:first sg:person.0756670103.21
    125 rdf:rest rdf:nil
    126 Nd4ed355afef64bc2a124c5f775c0851d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name DNA
    128 rdf:type schema:DefinedTerm
    129 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    130 schema:name Biological Sciences
    131 rdf:type schema:DefinedTerm
    132 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    133 schema:name Genetics
    134 rdf:type schema:DefinedTerm
    135 sg:journal.1105638 schema:issn 0969-7128
    136 1476-5462
    137 schema:name Gene Therapy
    138 rdf:type schema:Periodical
    139 sg:person.016203141374.94 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
    140 schema:familyName Song
    141 schema:givenName Y K
    142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016203141374.94
    143 rdf:type schema:Person
    144 sg:person.0615252507.11 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
    145 schema:familyName Liu
    146 schema:givenName F
    147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615252507.11
    148 rdf:type schema:Person
    149 sg:person.0756670103.21 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
    150 schema:familyName Liu
    151 schema:givenName D
    152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756670103.21
    153 rdf:type schema:Person
    154 sg:pub.10.1007/978-3-642-75608-5_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044683372
    155 https://doi.org/10.1007/978-3-642-75608-5_3
    156 rdf:type schema:CreativeWork
    157 sg:pub.10.1007/978-3-642-75608-5_4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002493894
    158 https://doi.org/10.1007/978-3-642-75608-5_4
    159 rdf:type schema:CreativeWork
    160 sg:pub.10.1007/978-3-642-75608-5_5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008839676
    161 https://doi.org/10.1007/978-3-642-75608-5_5
    162 rdf:type schema:CreativeWork
    163 sg:pub.10.1023/a:1016248701505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031603881
    164 https://doi.org/10.1023/a:1016248701505
    165 rdf:type schema:CreativeWork
    166 sg:pub.10.1038/sj.gt.3300424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031544602
    167 https://doi.org/10.1038/sj.gt.3300424
    168 rdf:type schema:CreativeWork
    169 https://app.dimensions.ai/details/publication/pub.1082423969 schema:CreativeWork
    170 https://app.dimensions.ai/details/publication/pub.1082478883 schema:CreativeWork
    171 https://app.dimensions.ai/details/publication/pub.1082983975 schema:CreativeWork
    172 https://doi.org/10.1006/jmcc.1997.0389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046679921
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1016/0076-6879(93)17090-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1015766739
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1016/0304-4165(81)90342-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034092280
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1016/0958-1669(92)90082-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1042420186
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1016/0959-437x(93)90126-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1012445290
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1016/b978-0-7506-9265-6.50021-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000943063
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1016/s0167-7799(97)01137-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044585682
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1089/hum.1991.2.1-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271037
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1089/hum.1993.4.5-643 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271261
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1089/hum.1994.5.12-1477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271343
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1089/hum.1996.7.5-637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271809
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1089/hum.1997.8.14-1659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271920
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1089/hum.1997.8.15-1763 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271932
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1111/j.1749-6632.1994.tb21703.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042784167
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1126/science.1690918 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062500820
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1126/science.272.5259.263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062552729
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1146/annurev.ne.19.030196.001405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035845383
    205 rdf:type schema:CreativeWork
    206 https://www.grid.ac/institutes/grid.21925.3d schema:alternateName University of Pittsburgh
    207 schema:name Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    208 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...