Characterization of cationic lipid-protamine–DNA (LPD) complexes for intravenous gene delivery View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1998-07

AUTHORS

S Li, MA Rizzo, S Bhattacharya, L Huang

ABSTRACT

A previous study has shown an efficient, systemic transgene expression in mice via intravenous administration of a LPD formulation composed of DOTAP liposomes, protamine sulfate and plasmid DNA. In this study, factors affecting the in vivo performance of this formulation were further evaluated. A protocol in which liposomes were mixed with protamine before the addition of plasmid DNA was shown to produce small condensed particles with a diameter of about 135 nm. These particles were stable over time and gave a high level of gene expression in all tissues examined including lung, heart, spleen, liver and kidney with the highest level of expression in the lung. Inclusion of dioleoylphosphatidylethanolamine (DOPE) as a helper lipid significantly decreased the in vivo activity of LPD. In contrast, inclusion of cholesterol as a helper lipid increased the in vivo transfection efficiency of LPD and more importantly, decrease the amount of cationic lipid required for the maximal level of gene expression. Studies on the interaction between mouse serum and LPD showed that LPD became negatively charged after exposure to serum, and LPDs containing different helper lipids varied in the amount of associated serum proteins. LPD containing DOPE was more enriched in a protein corresponding to albumin in molecular weight. These results suggest that the mechanism of LPD-mediated intravenous gene delivery might be different from that of in vitro lipofection and that serum protein association might be a major factor limiting the in vivo transfection by LPD. More... »

PAGES

3300683

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3300683

DOI

http://dx.doi.org/10.1038/sj.gt.3300683

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023825729

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9813664


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cations", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fatty Acids, Monounsaturated", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Injections, Intravenous", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liposomes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred Strains", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphatidylethanolamines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protamines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Quaternary Ammonium Compounds", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Laboratory of Drug Targeting, Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "S", 
        "id": "sg:person.015411531677.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015411531677.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Laboratory of Drug Targeting, Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rizzo", 
        "givenName": "MA", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Laboratory of Drug Targeting, Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhattacharya", 
        "givenName": "S", 
        "id": "sg:person.01036340343.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036340343.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Laboratory of Drug Targeting, Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "L", 
        "id": "sg:person.01005331112.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005331112.69"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0014-5793(96)01397-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007844889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.21.11454", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010497377"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014791369", 
          "https://doi.org/10.1038/sj.gt.3300482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014791369", 
          "https://doi.org/10.1038/sj.gt.3300482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0797-620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018747768", 
          "https://doi.org/10.1038/nbt0797-620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.14.7305", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026219069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(84)90782-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028017476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0797-647", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028041171", 
          "https://doi.org/10.1038/nbt0797-647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-3659(97)00043-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028428333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031544602", 
          "https://doi.org/10.1038/sj.gt.3300424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031544602", 
          "https://doi.org/10.1038/sj.gt.3300424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(96)00003-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045144856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4157(92)90039-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045774302"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4157(92)90039-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045774302"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi952436a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi952436a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1995.6.9-1129", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1996.7.14-1701", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1996.7.15-1791", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1996.7.15-1803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271709"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1997.8.5-511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059272021"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7687073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062648402"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1998-07", 
    "datePublishedReg": "1998-07-01", 
    "description": "A previous study has shown an efficient, systemic transgene expression in mice via intravenous administration of a LPD formulation composed of DOTAP liposomes, protamine sulfate and plasmid DNA. In this study, factors affecting the in vivo performance of this formulation were further evaluated. A protocol in which liposomes were mixed with protamine before the addition of plasmid DNA was shown to produce small condensed particles with a diameter of about 135 nm. These particles were stable over time and gave a high level of gene expression in all tissues examined including lung, heart, spleen, liver and kidney with the highest level of expression in the lung. Inclusion of dioleoylphosphatidylethanolamine (DOPE) as a helper lipid significantly decreased the in vivo activity of LPD. In contrast, inclusion of cholesterol as a helper lipid increased the in vivo transfection efficiency of LPD and more importantly, decrease the amount of cationic lipid required for the maximal level of gene expression. Studies on the interaction between mouse serum and LPD showed that LPD became negatively charged after exposure to serum, and LPDs containing different helper lipids varied in the amount of associated serum proteins. LPD containing DOPE was more enriched in a protein corresponding to albumin in molecular weight. These results suggest that the mechanism of LPD-mediated intravenous gene delivery might be different from that of in vitro lipofection and that serum protein association might be a major factor limiting the in vivo transfection by LPD.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.gt.3300683", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2472090", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2436124", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2435629", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "Characterization of cationic lipid-protamine\u2013DNA (LPD) complexes for intravenous gene delivery", 
    "pagination": "3300683", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7182884a2609932f82cdc876902bb0f37e5d91e874886a8d4eda7fea44cc3c4f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9813664"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3300683"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1023825729"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3300683", 
      "https://app.dimensions.ai/details/publication/pub.1023825729"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29194_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/3300683"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300683'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300683'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300683'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300683'


 

This table displays all metadata directly associated to this object as RDF triples.

205 TRIPLES      21 PREDICATES      60 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3300683 schema:about N08b7cabaef6e41ac9a56cad0f5483982
2 N0957fc4156ec4448888be41674a3f776
3 N2ba4d70ac1714540b1365b4864067820
4 N3f19fea812434bffb6f8e65c39ede17e
5 N5d063eff398042f68899488a03aa65ca
6 N646b0cc609544f6b9cb50a1c463904c8
7 N6876533c7e4344bd93fd9549f3fc1cc6
8 Na6cdc5e5d3324f2fa52dc0638e5255af
9 Nb74c633d96b9434b9c4393078d7def2c
10 Nc542b3235ed84a4a8768b931d8f5cf5a
11 Ncb60966ce9b343abb25737644a783777
12 Ne99f066304f541768270460aaea8b257
13 Nf45c164902324d77a3be6cebb88dcc1b
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author N18f9685400e2437397cc8aaad5a6c48e
17 schema:citation sg:pub.10.1038/nbt0797-620
18 sg:pub.10.1038/nbt0797-647
19 sg:pub.10.1038/sj.gt.3300424
20 sg:pub.10.1038/sj.gt.3300482
21 https://doi.org/10.1016/0003-2697(84)90782-6
22 https://doi.org/10.1016/0005-2736(96)00003-x
23 https://doi.org/10.1016/0304-4157(92)90039-d
24 https://doi.org/10.1016/s0014-5793(96)01397-x
25 https://doi.org/10.1016/s0168-3659(97)00043-6
26 https://doi.org/10.1021/bi952436a
27 https://doi.org/10.1073/pnas.93.14.7305
28 https://doi.org/10.1073/pnas.93.21.11454
29 https://doi.org/10.1089/hum.1995.6.9-1129
30 https://doi.org/10.1089/hum.1996.7.14-1701
31 https://doi.org/10.1089/hum.1996.7.15-1791
32 https://doi.org/10.1089/hum.1996.7.15-1803
33 https://doi.org/10.1089/hum.1997.8.5-511
34 https://doi.org/10.1126/science.7687073
35 schema:datePublished 1998-07
36 schema:datePublishedReg 1998-07-01
37 schema:description A previous study has shown an efficient, systemic transgene expression in mice via intravenous administration of a LPD formulation composed of DOTAP liposomes, protamine sulfate and plasmid DNA. In this study, factors affecting the in vivo performance of this formulation were further evaluated. A protocol in which liposomes were mixed with protamine before the addition of plasmid DNA was shown to produce small condensed particles with a diameter of about 135 nm. These particles were stable over time and gave a high level of gene expression in all tissues examined including lung, heart, spleen, liver and kidney with the highest level of expression in the lung. Inclusion of dioleoylphosphatidylethanolamine (DOPE) as a helper lipid significantly decreased the in vivo activity of LPD. In contrast, inclusion of cholesterol as a helper lipid increased the in vivo transfection efficiency of LPD and more importantly, decrease the amount of cationic lipid required for the maximal level of gene expression. Studies on the interaction between mouse serum and LPD showed that LPD became negatively charged after exposure to serum, and LPDs containing different helper lipids varied in the amount of associated serum proteins. LPD containing DOPE was more enriched in a protein corresponding to albumin in molecular weight. These results suggest that the mechanism of LPD-mediated intravenous gene delivery might be different from that of in vitro lipofection and that serum protein association might be a major factor limiting the in vivo transfection by LPD.
38 schema:genre research_article
39 schema:inLanguage en
40 schema:isAccessibleForFree true
41 schema:isPartOf N3f75fd85a4934e49b1a10909b5d8b881
42 N952cffb940fc4a2e94a10d76695efb28
43 sg:journal.1105638
44 schema:name Characterization of cationic lipid-protamine–DNA (LPD) complexes for intravenous gene delivery
45 schema:pagination 3300683
46 schema:productId N0b3c25b2eaea4ed79de9aa359a25ceab
47 N30a360fc3d9a4271af217b2fd935f7c7
48 Na06ea77b742f4688bb99d4e195cbb00b
49 Nb520230b261e4144a3b83de8d9d57d09
50 Nbdeb8c5d79ec4b4180f7be9b271c7d00
51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023825729
52 https://doi.org/10.1038/sj.gt.3300683
53 schema:sdDatePublished 2019-04-11T11:52
54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
55 schema:sdPublisher Ne14b20f8db8b4c7bb9ba4c2386c2fe73
56 schema:url http://www.nature.com/articles/3300683
57 sgo:license sg:explorer/license/
58 sgo:sdDataset articles
59 rdf:type schema:ScholarlyArticle
60 N08b7cabaef6e41ac9a56cad0f5483982 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name Liposomes
62 rdf:type schema:DefinedTerm
63 N0957fc4156ec4448888be41674a3f776 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Protamines
65 rdf:type schema:DefinedTerm
66 N0b3c25b2eaea4ed79de9aa359a25ceab schema:name readcube_id
67 schema:value 7182884a2609932f82cdc876902bb0f37e5d91e874886a8d4eda7fea44cc3c4f
68 rdf:type schema:PropertyValue
69 N18f9685400e2437397cc8aaad5a6c48e rdf:first sg:person.015411531677.33
70 rdf:rest N32a9bdbbead14545bc12ea9d71489d65
71 N2ba4d70ac1714540b1365b4864067820 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Gene Transfer Techniques
73 rdf:type schema:DefinedTerm
74 N30a360fc3d9a4271af217b2fd935f7c7 schema:name nlm_unique_id
75 schema:value 9421525
76 rdf:type schema:PropertyValue
77 N32a9bdbbead14545bc12ea9d71489d65 rdf:first Ne9c7d8f0863e483cb584ca57ace79f2b
78 rdf:rest Ne9e72f69db7844b0814e5316ec350db6
79 N3f19fea812434bffb6f8e65c39ede17e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Animals
81 rdf:type schema:DefinedTerm
82 N3f75fd85a4934e49b1a10909b5d8b881 schema:volumeNumber 5
83 rdf:type schema:PublicationVolume
84 N5d063eff398042f68899488a03aa65ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Quaternary Ammonium Compounds
86 rdf:type schema:DefinedTerm
87 N646b0cc609544f6b9cb50a1c463904c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Female
89 rdf:type schema:DefinedTerm
90 N6876533c7e4344bd93fd9549f3fc1cc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Mice, Inbred Strains
92 rdf:type schema:DefinedTerm
93 N81a6a6b269f44d4780c9deb98aeda964 rdf:first sg:person.01005331112.69
94 rdf:rest rdf:nil
95 N952cffb940fc4a2e94a10d76695efb28 schema:issueNumber 7
96 rdf:type schema:PublicationIssue
97 Na06ea77b742f4688bb99d4e195cbb00b schema:name doi
98 schema:value 10.1038/sj.gt.3300683
99 rdf:type schema:PropertyValue
100 Na6cdc5e5d3324f2fa52dc0638e5255af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Mice
102 rdf:type schema:DefinedTerm
103 Nb520230b261e4144a3b83de8d9d57d09 schema:name pubmed_id
104 schema:value 9813664
105 rdf:type schema:PropertyValue
106 Nb74c633d96b9434b9c4393078d7def2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Phosphatidylethanolamines
108 rdf:type schema:DefinedTerm
109 Nbdeb8c5d79ec4b4180f7be9b271c7d00 schema:name dimensions_id
110 schema:value pub.1023825729
111 rdf:type schema:PropertyValue
112 Nc542b3235ed84a4a8768b931d8f5cf5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Plasmids
114 rdf:type schema:DefinedTerm
115 Ncb60966ce9b343abb25737644a783777 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Cations
117 rdf:type schema:DefinedTerm
118 Ne14b20f8db8b4c7bb9ba4c2386c2fe73 schema:name Springer Nature - SN SciGraph project
119 rdf:type schema:Organization
120 Ne99f066304f541768270460aaea8b257 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Injections, Intravenous
122 rdf:type schema:DefinedTerm
123 Ne9c7d8f0863e483cb584ca57ace79f2b schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
124 schema:familyName Rizzo
125 schema:givenName MA
126 rdf:type schema:Person
127 Ne9e72f69db7844b0814e5316ec350db6 rdf:first sg:person.01036340343.43
128 rdf:rest N81a6a6b269f44d4780c9deb98aeda964
129 Nf45c164902324d77a3be6cebb88dcc1b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Fatty Acids, Monounsaturated
131 rdf:type schema:DefinedTerm
132 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
133 schema:name Biological Sciences
134 rdf:type schema:DefinedTerm
135 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
136 schema:name Genetics
137 rdf:type schema:DefinedTerm
138 sg:grant.2435629 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3300683
139 rdf:type schema:MonetaryGrant
140 sg:grant.2436124 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3300683
141 rdf:type schema:MonetaryGrant
142 sg:grant.2472090 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3300683
143 rdf:type schema:MonetaryGrant
144 sg:journal.1105638 schema:issn 0969-7128
145 1476-5462
146 schema:name Gene Therapy
147 rdf:type schema:Periodical
148 sg:person.01005331112.69 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
149 schema:familyName Huang
150 schema:givenName L
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005331112.69
152 rdf:type schema:Person
153 sg:person.01036340343.43 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
154 schema:familyName Bhattacharya
155 schema:givenName S
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036340343.43
157 rdf:type schema:Person
158 sg:person.015411531677.33 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
159 schema:familyName Li
160 schema:givenName S
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015411531677.33
162 rdf:type schema:Person
163 sg:pub.10.1038/nbt0797-620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018747768
164 https://doi.org/10.1038/nbt0797-620
165 rdf:type schema:CreativeWork
166 sg:pub.10.1038/nbt0797-647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028041171
167 https://doi.org/10.1038/nbt0797-647
168 rdf:type schema:CreativeWork
169 sg:pub.10.1038/sj.gt.3300424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031544602
170 https://doi.org/10.1038/sj.gt.3300424
171 rdf:type schema:CreativeWork
172 sg:pub.10.1038/sj.gt.3300482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014791369
173 https://doi.org/10.1038/sj.gt.3300482
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/0003-2697(84)90782-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028017476
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/0005-2736(96)00003-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1045144856
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/0304-4157(92)90039-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1045774302
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/s0014-5793(96)01397-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007844889
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/s0168-3659(97)00043-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028428333
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1021/bi952436a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055211114
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1073/pnas.93.14.7305 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026219069
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1073/pnas.93.21.11454 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010497377
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1089/hum.1995.6.9-1129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271621
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1089/hum.1996.7.14-1701 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271698
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1089/hum.1996.7.15-1791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271707
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1089/hum.1996.7.15-1803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271709
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1089/hum.1997.8.5-511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272021
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1126/science.7687073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062648402
202 rdf:type schema:CreativeWork
203 https://www.grid.ac/institutes/grid.21925.3d schema:alternateName University of Pittsburgh
204 schema:name Laboratory of Drug Targeting, Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
205 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...