Therapeutic gene targeting View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1998-02

AUTHORS

R J Yáñez, A C Porter

ABSTRACT

Gene targeting is the use of homologous recombination to make defined alterations to the genome. One of the possible outcomes of gene targeting is the accurate correction of genetic defects, and this would make it the ideal method of gene therapy for single gene disorder. While gene targeting has been achieved both in human cell lines and in nontransformed, primary human cells, its low efficiency has been a major limitation to its therapeutic potential. Gene therapy in vivo gene targeting is there for impractical without dramatic improvements in targeting efficiency. Ex vivo approaches might more realistically be considered, but would benefit from progress in the isolation and growth of somatic stem cells and improvements in targeting efficiency. We provide here a brief review of the challenges of gene therapy by gene targeting. This is followed by a critical overview of recent developments in gene targeting techniques, and in our understanding of the underlying processes of homologous and nonhomologous recombination. More... »

PAGES

3300601

References to SciGraph publications

  • 1992-12. Targeted breakage of a human chromosome mediated by cloned human telomeric DNA in NATURE GENETICS
  • 1993-06. Mismatch recognition in chromosomal interactions and speciation in CHROMOSOMA
  • 1993-07. Targeting vector configuration and method of gene transfer influence targeted correction of theAPRT gene in Chinese hamster ovary cells in SOMATIC CELL AND MOLECULAR GENETICS
  • 1996-11. PCR-based gene targeting of the inducible nitric oxide synthase (NOS2) locus in murine ES cells, a new and more cost-effective approach in TRANSGENIC RESEARCH
  • 1985-09. Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination in NATURE
  • 1997-05. Interaction between ATM protein and c-Abl in response to DNA damage in NATURE
  • 1997-05. A sense-abl response? in NATURE
  • 1994-01. Gene targeting in normal somatic cells: inactivation of the interferon–γ receptor in myoblasts in NATURE GENETICS
  • 1994-03. Gene conversion in the chicken immunoglobulin locus: A paradigm of homologous recombination in higher eukaryotes in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 1989-03. Production of chimaeric mice containing embryonic stem (ES) cells carrying a homoeobox Hox 1.1 allele mutated by homologous recombination in NATURE
  • 1996-02. Efficient modification of human chromosomal alleles using recombination-proficient chicken/human microcell hybrids in NATURE GENETICS
  • 1997-09. Gene therapy - promises, problems and prospects in NATURE
  • 1997-03. Construction by gene targeting in human cells of a ‘conditional’ CDC2 mutant that rereplicates its DNA in NATURE GENETICS
  • 1997-07. Efficient gene targeting in mouse embryonic stem cells in GENE THERAPY
  • 1997-04. Functional interaction between DNA-PK and c-Abl in response to DNA damage in NATURE
  • Journal

    TITLE

    Gene Therapy

    ISSUE

    2

    VOLUME

    5

    Author Affiliations

    Related Patents

  • Methods Of Improving Homologous Recombination
  • Knockout Mouse For The Tumor Suppressor Gene Anx7
  • Targeted Chromosomal Mutagenasis Using Zinc Finger Nucleases
  • Targeted Gene Modification By Parvoviral Vectors
  • Regulation Of Endogenous Gene Expression In Cells Using Zinc Finger Proteins
  • Methods And Compostions For Targeted Genomic Deletion
  • Targeted Chromosomal Mutagenesis Using Zinc Finger Nucleases
  • Humanized Monoclonal Antibodies From A Transgenic Rat
  • Ng2/Hm Proteoglycan-Binding Peptides That Home To Angiogenic Vasculature And Related Methods
  • Method Of Inactivating A Glucocorticoid Receptor Gene In An Isolated Cell
  • Use Of Chimeric Nucleases To Stimulate Gene Targeting
  • Targeted Chromosomal Genomic Alterations With Modified Single Stranded Oligonucleotides
  • Method For Treating Oncological Diseases
  • Methods And Compositions For Targeted Gene Modification
  • Methods And Compositions For Targeted Genomic Deletion
  • Gene Targeting Vector, And Method For Using Same
  • Targeted Gene Modification By Parvoviral Vectors
  • Zinc Finger Proteins And Method For Inactivating A Dhfr Gene In A Chinese Hamster Ovary Cell
  • Use Of Chimeric Nucleases To Stimulate Gene Targeting
  • Compositions Comprising Genome Segments And Methods Of Using The Same
  • Algorithm For Modification Of Somatic Cancer Evolution
  • Repressing Endogenous Ccr5 Gene Expression In Cells Using Engineered Zinc Finger Proteins
  • Compositions And Methods For Enhancing Oligonucleotide-Mediated Gene Alteration
  • Targeted Chromosomal Genomic Alterations With Modified Single Stranded Oligonucleotides
  • Targeted Integration And Expression On Exogenous Nucleic Acid Sequences
  • Targeted Gene Modification By Parvoviral Vectors
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.gt.3300601

    DOI

    http://dx.doi.org/10.1038/sj.gt.3300601

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043341032

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9578833


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adenoviridae", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA-Binding Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Targeting", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Therapy", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombination, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Imperial College London", 
              "id": "https://www.grid.ac/institutes/grid.7445.2", 
              "name": [
                "Gene Targeting Group, Imperial College School of Medicine, Hammersmith Hospital, London, UK."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Y\u00e1\u00f1ez", 
            "givenName": "R J", 
            "id": "sg:person.01352562644.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352562644.09"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Porter", 
            "givenName": "A C", 
            "id": "sg:person.010560307572.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010560307572.27"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf01924010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000337247", 
              "https://doi.org/10.1007/bf01924010"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00360400", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000577159", 
              "https://doi.org/10.1007/bf00360400"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.87.4.1566", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001465037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.7.6.2294", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001838360"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/387450a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001988850", 
              "https://doi.org/10.1038/387450a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3300457", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003072328", 
              "https://doi.org/10.1038/sj.gt.3300457"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3300457", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003072328", 
              "https://doi.org/10.1038/sj.gt.3300457"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/317230a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005647127", 
              "https://doi.org/10.1038/317230a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.12.8.3365", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006806233"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/386732a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008042972", 
              "https://doi.org/10.1038/386732a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/excr.1996.3470", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008478164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(00)80199-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010740055"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1292-283", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011756318", 
              "https://doi.org/10.1038/ng1292-283"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/18.20.5981", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012643497"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.16.12.6820", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012990469"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/21.3.407", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017042523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0296-174", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017853247", 
              "https://doi.org/10.1038/ng0296-174"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.92.17.7844", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018395893"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/338150a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019082387", 
              "https://doi.org/10.1038/338150a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01980206", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022699927", 
              "https://doi.org/10.1007/bf01980206"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01980206", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022699927", 
              "https://doi.org/10.1007/bf01980206"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/23.24.5012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024116167"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.4.2.157", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024182286"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.10.1.37", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024238202"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0194-90", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024528923", 
              "https://doi.org/10.1038/ng0194-90"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.ge.29.120195.002453", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025954194"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.94.7.2987", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026143688"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.25.11943", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028825083"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/387520a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029483387", 
              "https://doi.org/10.1038/387520a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.10.9.4837", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029954885"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(00)80198-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032203002"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(91)90581-i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033459891"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(95)90319-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034080169"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.18.8067", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034996860"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.8.1.196", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035885908"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(85)90242-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036830078"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/16.23.11319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037040408"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0968-0004(00)89085-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037505363"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bies.950160907", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038048241"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0397-258", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038486934", 
              "https://doi.org/10.1038/ng0397-258"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.17.1.267", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039424106"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/jcs.1988.supplement_10.7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039494147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.90.21.9832", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040085435"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0968-0004(00)89089-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040354650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/mrd.1080360404", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040657074"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.13.2.918", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042687581"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.10.4.1799", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043089326"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/22.25.5649", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043467871"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/19.14.3835", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045162647"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.92.20.9161", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045517832"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-4652(199703)170:3<299::aid-jcp11>3.0.co;2-a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045531790"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01232748", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046060918", 
              "https://doi.org/10.1007/bf01232748"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.8.12.5350", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046403243"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0027-5107(86)90038-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047339994"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0921-8777(92)90038-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048255176"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0921-8777(92)90038-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048255176"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/38410", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048288589", 
              "https://doi.org/10.1038/38410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/38410", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048288589", 
              "https://doi.org/10.1038/38410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0027-5107(91)90145-e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049392697"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/sqb.1984.049.01.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060405567"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2660260", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062549377"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.277.5327.831", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062557618"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.6093246", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062634093"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.7801122", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062649753"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.8465203", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062655691"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1998-02", 
        "datePublishedReg": "1998-02-01", 
        "description": "Gene targeting is the use of homologous recombination to make defined alterations to the genome. One of the possible outcomes of gene targeting is the accurate correction of genetic defects, and this would make it the ideal method of gene therapy for single gene disorder. While gene targeting has been achieved both in human cell lines and in nontransformed, primary human cells, its low efficiency has been a major limitation to its therapeutic potential. Gene therapy in vivo gene targeting is there for impractical without dramatic improvements in targeting efficiency. Ex vivo approaches might more realistically be considered, but would benefit from progress in the isolation and growth of somatic stem cells and improvements in targeting efficiency. We provide here a brief review of the challenges of gene therapy by gene targeting. This is followed by a critical overview of recent developments in gene targeting techniques, and in our understanding of the underlying processes of homologous and nonhomologous recombination.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/sj.gt.3300601", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1105638", 
            "issn": [
              "0969-7128", 
              "1476-5462"
            ], 
            "name": "Gene Therapy", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "5"
          }
        ], 
        "name": "Therapeutic gene targeting", 
        "pagination": "3300601", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "ce7347f67d1d9b8ec87bb98f7be3008f855bef9b3327df66e9ddcce4f0d19d66"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "9578833"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9421525"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/sj.gt.3300601"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1043341032"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/sj.gt.3300601", 
          "https://app.dimensions.ai/details/publication/pub.1043341032"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T11:53", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29197_00000002.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/3300601"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300601'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300601'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300601'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300601'


     

    This table displays all metadata directly associated to this object as RDF triples.

    309 TRIPLES      21 PREDICATES      99 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/sj.gt.3300601 schema:about N0ee734acac36480fabb3a666d5031383
    2 N52e5b860a02548209cb6325705cc2dc9
    3 N6442cf77574b482cbbf951bd61fe649a
    4 N9b65546747db4f10a20e126814951b55
    5 Na81360a92e01495d865e94c1559dd521
    6 Nd622e5871ae640a084e3f43d0c0235b3
    7 Ne28ae2f806ee4454b70e1f0df2a5e77d
    8 Ne3f9638d05df439ea1523f3b6aa3a326
    9 Nf86249e2a8784867a49aa88186304565
    10 anzsrc-for:06
    11 anzsrc-for:0604
    12 schema:author N71a8e05c86594657a4cb6657504a2bae
    13 schema:citation sg:pub.10.1007/bf00360400
    14 sg:pub.10.1007/bf01232748
    15 sg:pub.10.1007/bf01924010
    16 sg:pub.10.1007/bf01980206
    17 sg:pub.10.1038/317230a0
    18 sg:pub.10.1038/338150a0
    19 sg:pub.10.1038/38410
    20 sg:pub.10.1038/386732a0
    21 sg:pub.10.1038/387450a0
    22 sg:pub.10.1038/387520a0
    23 sg:pub.10.1038/ng0194-90
    24 sg:pub.10.1038/ng0296-174
    25 sg:pub.10.1038/ng0397-258
    26 sg:pub.10.1038/ng1292-283
    27 sg:pub.10.1038/sj.gt.3300457
    28 https://doi.org/10.1002/(sici)1097-4652(199703)170:3<299::aid-jcp11>3.0.co;2-a
    29 https://doi.org/10.1002/bies.950160907
    30 https://doi.org/10.1002/mrd.1080360404
    31 https://doi.org/10.1006/excr.1996.3470
    32 https://doi.org/10.1016/0027-5107(86)90038-2
    33 https://doi.org/10.1016/0027-5107(91)90145-e
    34 https://doi.org/10.1016/0092-8674(85)90242-9
    35 https://doi.org/10.1016/0092-8674(91)90581-i
    36 https://doi.org/10.1016/0092-8674(95)90319-4
    37 https://doi.org/10.1016/0921-8777(92)90038-5
    38 https://doi.org/10.1016/s0092-8674(00)80198-1
    39 https://doi.org/10.1016/s0092-8674(00)80199-3
    40 https://doi.org/10.1016/s0968-0004(00)89085-4
    41 https://doi.org/10.1016/s0968-0004(00)89089-1
    42 https://doi.org/10.1073/pnas.87.4.1566
    43 https://doi.org/10.1073/pnas.88.18.8067
    44 https://doi.org/10.1073/pnas.90.21.9832
    45 https://doi.org/10.1073/pnas.91.25.11943
    46 https://doi.org/10.1073/pnas.92.17.7844
    47 https://doi.org/10.1073/pnas.92.20.9161
    48 https://doi.org/10.1073/pnas.94.7.2987
    49 https://doi.org/10.1093/nar/16.23.11319
    50 https://doi.org/10.1093/nar/18.20.5981
    51 https://doi.org/10.1093/nar/19.14.3835
    52 https://doi.org/10.1093/nar/21.3.407
    53 https://doi.org/10.1093/nar/22.25.5649
    54 https://doi.org/10.1093/nar/23.24.5012
    55 https://doi.org/10.1101/gad.4.2.157
    56 https://doi.org/10.1101/sqb.1984.049.01.019
    57 https://doi.org/10.1126/science.2660260
    58 https://doi.org/10.1126/science.277.5327.831
    59 https://doi.org/10.1126/science.6093246
    60 https://doi.org/10.1126/science.7801122
    61 https://doi.org/10.1126/science.8465203
    62 https://doi.org/10.1128/mcb.10.1.37
    63 https://doi.org/10.1128/mcb.10.4.1799
    64 https://doi.org/10.1128/mcb.10.9.4837
    65 https://doi.org/10.1128/mcb.12.8.3365
    66 https://doi.org/10.1128/mcb.13.2.918
    67 https://doi.org/10.1128/mcb.16.12.6820
    68 https://doi.org/10.1128/mcb.17.1.267
    69 https://doi.org/10.1128/mcb.7.6.2294
    70 https://doi.org/10.1128/mcb.8.1.196
    71 https://doi.org/10.1128/mcb.8.12.5350
    72 https://doi.org/10.1146/annurev.ge.29.120195.002453
    73 https://doi.org/10.1242/jcs.1988.supplement_10.7
    74 schema:datePublished 1998-02
    75 schema:datePublishedReg 1998-02-01
    76 schema:description Gene targeting is the use of homologous recombination to make defined alterations to the genome. One of the possible outcomes of gene targeting is the accurate correction of genetic defects, and this would make it the ideal method of gene therapy for single gene disorder. While gene targeting has been achieved both in human cell lines and in nontransformed, primary human cells, its low efficiency has been a major limitation to its therapeutic potential. Gene therapy in vivo gene targeting is there for impractical without dramatic improvements in targeting efficiency. Ex vivo approaches might more realistically be considered, but would benefit from progress in the isolation and growth of somatic stem cells and improvements in targeting efficiency. We provide here a brief review of the challenges of gene therapy by gene targeting. This is followed by a critical overview of recent developments in gene targeting techniques, and in our understanding of the underlying processes of homologous and nonhomologous recombination.
    77 schema:genre research_article
    78 schema:inLanguage en
    79 schema:isAccessibleForFree true
    80 schema:isPartOf Na9fb836e47d443d18ffa291fde2132d1
    81 Nf771bc1048b84d85a79bee9d8fd09dd9
    82 sg:journal.1105638
    83 schema:name Therapeutic gene targeting
    84 schema:pagination 3300601
    85 schema:productId N41fdd36efe5f4addac7b76a34caf0f47
    86 N50df17bcd72d4951ac254d83563236ee
    87 N61586f095088444ebbcaefc9c9be3256
    88 N82a954a78dd241509a445eab3d93e45c
    89 Na61895b7edf14c1ea00ba836062a0aff
    90 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043341032
    91 https://doi.org/10.1038/sj.gt.3300601
    92 schema:sdDatePublished 2019-04-11T11:53
    93 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    94 schema:sdPublisher N177d25351b0448dab0f67bf7cb6e2f65
    95 schema:url http://www.nature.com/articles/3300601
    96 sgo:license sg:explorer/license/
    97 sgo:sdDataset articles
    98 rdf:type schema:ScholarlyArticle
    99 N05179c0d64204f41961a8f44d28c9d08 rdf:first sg:person.010560307572.27
    100 rdf:rest rdf:nil
    101 N0ee734acac36480fabb3a666d5031383 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Genetic Therapy
    103 rdf:type schema:DefinedTerm
    104 N177d25351b0448dab0f67bf7cb6e2f65 schema:name Springer Nature - SN SciGraph project
    105 rdf:type schema:Organization
    106 N41fdd36efe5f4addac7b76a34caf0f47 schema:name nlm_unique_id
    107 schema:value 9421525
    108 rdf:type schema:PropertyValue
    109 N50df17bcd72d4951ac254d83563236ee schema:name doi
    110 schema:value 10.1038/sj.gt.3300601
    111 rdf:type schema:PropertyValue
    112 N52e5b860a02548209cb6325705cc2dc9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Humans
    114 rdf:type schema:DefinedTerm
    115 N61586f095088444ebbcaefc9c9be3256 schema:name readcube_id
    116 schema:value ce7347f67d1d9b8ec87bb98f7be3008f855bef9b3327df66e9ddcce4f0d19d66
    117 rdf:type schema:PropertyValue
    118 N6442cf77574b482cbbf951bd61fe649a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Recombination, Genetic
    120 rdf:type schema:DefinedTerm
    121 N71a8e05c86594657a4cb6657504a2bae rdf:first sg:person.01352562644.09
    122 rdf:rest N05179c0d64204f41961a8f44d28c9d08
    123 N82a954a78dd241509a445eab3d93e45c schema:name pubmed_id
    124 schema:value 9578833
    125 rdf:type schema:PropertyValue
    126 N9b65546747db4f10a20e126814951b55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name DNA-Binding Proteins
    128 rdf:type schema:DefinedTerm
    129 Na61895b7edf14c1ea00ba836062a0aff schema:name dimensions_id
    130 schema:value pub.1043341032
    131 rdf:type schema:PropertyValue
    132 Na81360a92e01495d865e94c1559dd521 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Animals
    134 rdf:type schema:DefinedTerm
    135 Na9fb836e47d443d18ffa291fde2132d1 schema:volumeNumber 5
    136 rdf:type schema:PublicationVolume
    137 Nd622e5871ae640a084e3f43d0c0235b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Adenoviridae
    139 rdf:type schema:DefinedTerm
    140 Ne28ae2f806ee4454b70e1f0df2a5e77d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Genetic Vectors
    142 rdf:type schema:DefinedTerm
    143 Ne3f9638d05df439ea1523f3b6aa3a326 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Stem Cells
    145 rdf:type schema:DefinedTerm
    146 Nf771bc1048b84d85a79bee9d8fd09dd9 schema:issueNumber 2
    147 rdf:type schema:PublicationIssue
    148 Nf86249e2a8784867a49aa88186304565 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Gene Targeting
    150 rdf:type schema:DefinedTerm
    151 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    152 schema:name Biological Sciences
    153 rdf:type schema:DefinedTerm
    154 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Genetics
    156 rdf:type schema:DefinedTerm
    157 sg:journal.1105638 schema:issn 0969-7128
    158 1476-5462
    159 schema:name Gene Therapy
    160 rdf:type schema:Periodical
    161 sg:person.010560307572.27 schema:familyName Porter
    162 schema:givenName A C
    163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010560307572.27
    164 rdf:type schema:Person
    165 sg:person.01352562644.09 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
    166 schema:familyName Yáñez
    167 schema:givenName R J
    168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352562644.09
    169 rdf:type schema:Person
    170 sg:pub.10.1007/bf00360400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000577159
    171 https://doi.org/10.1007/bf00360400
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1007/bf01232748 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046060918
    174 https://doi.org/10.1007/bf01232748
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1007/bf01924010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000337247
    177 https://doi.org/10.1007/bf01924010
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1007/bf01980206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022699927
    180 https://doi.org/10.1007/bf01980206
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1038/317230a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005647127
    183 https://doi.org/10.1038/317230a0
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1038/338150a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019082387
    186 https://doi.org/10.1038/338150a0
    187 rdf:type schema:CreativeWork
    188 sg:pub.10.1038/38410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048288589
    189 https://doi.org/10.1038/38410
    190 rdf:type schema:CreativeWork
    191 sg:pub.10.1038/386732a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008042972
    192 https://doi.org/10.1038/386732a0
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1038/387450a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001988850
    195 https://doi.org/10.1038/387450a0
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1038/387520a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029483387
    198 https://doi.org/10.1038/387520a0
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1038/ng0194-90 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024528923
    201 https://doi.org/10.1038/ng0194-90
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1038/ng0296-174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017853247
    204 https://doi.org/10.1038/ng0296-174
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1038/ng0397-258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038486934
    207 https://doi.org/10.1038/ng0397-258
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1038/ng1292-283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011756318
    210 https://doi.org/10.1038/ng1292-283
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1038/sj.gt.3300457 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003072328
    213 https://doi.org/10.1038/sj.gt.3300457
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1002/(sici)1097-4652(199703)170:3<299::aid-jcp11>3.0.co;2-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1045531790
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1002/bies.950160907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038048241
    218 rdf:type schema:CreativeWork
    219 https://doi.org/10.1002/mrd.1080360404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040657074
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1006/excr.1996.3470 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008478164
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.1016/0027-5107(86)90038-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047339994
    224 rdf:type schema:CreativeWork
    225 https://doi.org/10.1016/0027-5107(91)90145-e schema:sameAs https://app.dimensions.ai/details/publication/pub.1049392697
    226 rdf:type schema:CreativeWork
    227 https://doi.org/10.1016/0092-8674(85)90242-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036830078
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1016/0092-8674(91)90581-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1033459891
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1016/0092-8674(95)90319-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034080169
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1016/0921-8777(92)90038-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048255176
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1016/s0092-8674(00)80198-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032203002
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1016/s0092-8674(00)80199-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010740055
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1016/s0968-0004(00)89085-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037505363
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1016/s0968-0004(00)89089-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040354650
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1073/pnas.87.4.1566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001465037
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1073/pnas.88.18.8067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034996860
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1073/pnas.90.21.9832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040085435
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1073/pnas.91.25.11943 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028825083
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1073/pnas.92.17.7844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018395893
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1073/pnas.92.20.9161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045517832
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1073/pnas.94.7.2987 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026143688
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1093/nar/16.23.11319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037040408
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1093/nar/18.20.5981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012643497
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1093/nar/19.14.3835 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045162647
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1093/nar/21.3.407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017042523
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1093/nar/22.25.5649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043467871
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1093/nar/23.24.5012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024116167
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1101/gad.4.2.157 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024182286
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1101/sqb.1984.049.01.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060405567
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1126/science.2660260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062549377
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1126/science.277.5327.831 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062557618
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1126/science.6093246 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062634093
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1126/science.7801122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062649753
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1126/science.8465203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655691
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1128/mcb.10.1.37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024238202
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1128/mcb.10.4.1799 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043089326
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1128/mcb.10.9.4837 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029954885
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1128/mcb.12.8.3365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006806233
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1128/mcb.13.2.918 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042687581
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1128/mcb.16.12.6820 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012990469
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1128/mcb.17.1.267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039424106
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1128/mcb.7.6.2294 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001838360
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1128/mcb.8.1.196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035885908
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1128/mcb.8.12.5350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046403243
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1146/annurev.ge.29.120195.002453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025954194
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1242/jcs.1988.supplement_10.7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039494147
    306 rdf:type schema:CreativeWork
    307 https://www.grid.ac/institutes/grid.7445.2 schema:alternateName Imperial College London
    308 schema:name Gene Targeting Group, Imperial College School of Medicine, Hammersmith Hospital, London, UK.
    309 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...