Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1997-11

AUTHORS

D Klein, S Indraccolo, K von Rombs, A Amadori, B Salmons, WH Günzburg

ABSTRACT

Various methods for determining the expression of the β-galactosidase (β-gal) gene after retroviral transduction were compared as a means to assess retroviral titre. To allow better comparison, different retroviral vectors were constructed carrying two mutants of the green fluorescent protein and assessed as sensitive markers of retroviral gene transfer. It could be demonstrated that GFP is gener-ally superior to β-gal in terms of sensitivity, speed and noninvasiveness of assay, allowing easy direct FACS sorting of populations of transduced cells. This opens the possibility of enrichment by sorting of ex vivo transduced cells in gene therapy protocols. More... »

PAGES

1256-1260

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3300519

DOI

http://dx.doi.org/10.1038/sj.gt.3300519

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003690948

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9425450


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213 grid-institutes:grid.5608.b schema:alternateName Department of Oncology and Surgical Sciences, University of Padua, Padua, Italy
214 schema:name Department of Oncology and Surgical Sciences, University of Padua, Padua, Italy
215 IST-Biotechnology Section, Padua, Italy
216 rdf:type schema:Organization
 




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