In vivo gene transfer via intravenous administration of cationic lipid–protamine–DNA (LPD) complexes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1997-09

AUTHORS

S Li, L Huang

ABSTRACT

A novel LPD formulation has been developed for in vivo gene transfer. It involves the interaction of plasmid DNA with protamine sulfate, a cationic polypeptide, followed by the addition of DOTAP cationic liposomes. Compared with DOTAP/DNA complexes, LPD offers better protection of plasmid DNA against enzymatic digestion and gives consistently higher gene expression in mice via tail vein injection. When a luciferase reporter gene was employed, gene expression was found in all tissues examined including lung, heart, spleen, liver and kidney with the highest expression in the lung. The in vivo efficiency of LPD was dependent upon charge ratio and was also affected by the lipid used. Increasing the amount of DNA delivered induced an increase in gene expression. The optimal dose was approximately 50 micrograms per mouse at which concentration approximately 20 ng luciferase protein per milligram extracted tissue protein could be detected in the lung. Increasing the DNA to 100 micrograms per mouse resulted in toxicity and death of the animal. Gene expression in the lung was detected as early as 1 h after injection, peaked at 6 h and declined thereafter. High expression was also found in the spleen 6 h after injection but dropped very rapidly thereafter. The in vivo gene expression by LPD was dependent upon the route of administration since intraportal injection of LPD led to about a 100-fold decrease in gene expression in the lung as compared with i.v. injection. Using lacZ as a reporter gene, it was shown that endothelial cells were the primary locus of transgene expression in both the lung and spleen. No sign of inflammation in these organs was noticed. Since protamine sulfate has been proven to be nontoxic and only weakly immunogenic in humans, this novel vector may be useful for clinical gene therapy. More... »

PAGES

3300482

Journal

TITLE

Gene Therapy

ISSUE

9

VOLUME

4

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.gt.3300482

DOI

http://dx.doi.org/10.1038/sj.gt.3300482

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014791369

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9349425


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cations", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fatty Acids, Monounsaturated", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Injections, Intravenous", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lac Operon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liposomes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luciferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lung", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred Strains", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protamines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Quaternary Ammonium Compounds", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Department of Pharmacology, University of Pittsburgh School of Medicine, PA 15261, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "S", 
        "id": "sg:person.014614151277.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014614151277.22"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Huang", 
        "givenName": "L", 
        "id": "sg:person.01005331112.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005331112.69"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0005-2736(92)90316-e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001002731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(92)90316-e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001002731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/08982109309147441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001807459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(94)01315-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007571113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(96)01397-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007844889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.16.7297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018266629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/08982109609031138", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018600370"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.23.11307", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021985080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.21.7413", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022874269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.14.7305", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026219069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300484", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027651488", 
          "https://doi.org/10.1038/sj.gt.3300484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300484", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027651488", 
          "https://doi.org/10.1038/sj.gt.3300484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300485", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028276876", 
          "https://doi.org/10.1038/sj.gt.3300485"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300485", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028276876", 
          "https://doi.org/10.1038/sj.gt.3300485"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.7.2812", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030378744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031544602", 
          "https://doi.org/10.1038/sj.gt.3300424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031544602", 
          "https://doi.org/10.1038/sj.gt.3300424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4165(81)90342-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034092280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4165(81)90342-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034092280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0297-167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042860966", 
          "https://doi.org/10.1038/nbt0297-167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.32.18997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042873779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/357455a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044352249", 
          "https://doi.org/10.1038/357455a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi952436a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi952436a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9602019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9602019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja962713g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055865824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja962713g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055865824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1996.7.14-1701", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1996.7.15-1791", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1996.7.15-1803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271709"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1997-09", 
    "datePublishedReg": "1997-09-01", 
    "description": "A novel LPD formulation has been developed for in vivo gene transfer. It involves the interaction of plasmid DNA with protamine sulfate, a cationic polypeptide, followed by the addition of DOTAP cationic liposomes. Compared with DOTAP/DNA complexes, LPD offers better protection of plasmid DNA against enzymatic digestion and gives consistently higher gene expression in mice via tail vein injection. When a luciferase reporter gene was employed, gene expression was found in all tissues examined including lung, heart, spleen, liver and kidney with the highest expression in the lung. The in vivo efficiency of LPD was dependent upon charge ratio and was also affected by the lipid used. Increasing the amount of DNA delivered induced an increase in gene expression. The optimal dose was approximately 50 micrograms per mouse at which concentration approximately 20 ng luciferase protein per milligram extracted tissue protein could be detected in the lung. Increasing the DNA to 100 micrograms per mouse resulted in toxicity and death of the animal. Gene expression in the lung was detected as early as 1 h after injection, peaked at 6 h and declined thereafter. High expression was also found in the spleen 6 h after injection but dropped very rapidly thereafter. The in vivo gene expression by LPD was dependent upon the route of administration since intraportal injection of LPD led to about a 100-fold decrease in gene expression in the lung as compared with i.v. injection. Using lacZ as a reporter gene, it was shown that endothelial cells were the primary locus of transgene expression in both the lung and spleen. No sign of inflammation in these organs was noticed. Since protamine sulfate has been proven to be nontoxic and only weakly immunogenic in humans, this novel vector may be useful for clinical gene therapy.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.gt.3300482", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2472090", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2436124", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2435629", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "4"
      }
    ], 
    "name": "In vivo gene transfer via intravenous administration of cationic lipid\u2013protamine\u2013DNA (LPD) complexes", 
    "pagination": "3300482", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "46784b1f5153d02f18e38124c180202ca9a70c85a05f355e1c2e8885c128d8d1"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9349425"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.gt.3300482"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1014791369"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.gt.3300482", 
      "https://app.dimensions.ai/details/publication/pub.1014791369"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_54014_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/3300482"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300482'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300482'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300482'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.gt.3300482'


 

This table displays all metadata directly associated to this object as RDF triples.

227 TRIPLES      21 PREDICATES      70 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.gt.3300482 schema:about N023bb701a7314e5aa9681f72070f12f5
2 N09cf94eae98f4e71aa85352503470647
3 N381c28c7cbc34d3791f7019c1c78a291
4 N3beb10a63ece40c19ad5a166aba6e882
5 N472afb3b39bd4eb88a4345e915202aa0
6 N657b3516262246c8b62aa72b62f85cb9
7 N676ba1a90d08404eba9c2ed3c2155ade
8 N7bd6e7ef347145bca8e5c2950976e72b
9 N8417fa86a4b2458496357f7d1ca7a563
10 N87f6e26e70584881b9db2d55331beba1
11 N90419d8e7fb043b8953f80be9ad44f03
12 Nc48bfdfb76414a12a54a2a3792ed10e4
13 Nd0231b2bef804e288098d61a8a29db16
14 Nd62c92aecbae4ddaad7139d57ea0705c
15 Ne47f9486081d47a99120cd0fe7ff4081
16 Nf01cf587da564db78e6d939316649f94
17 Nf5570019f2a94eef8ad78a406b3cc18e
18 Nf62e2c32b0b046ac96b2fb05442ec445
19 anzsrc-for:06
20 anzsrc-for:0604
21 schema:author N5458163f5d1747d0abbec36201bd840c
22 schema:citation sg:pub.10.1038/357455a0
23 sg:pub.10.1038/nbt0297-167
24 sg:pub.10.1038/sj.gt.3300424
25 sg:pub.10.1038/sj.gt.3300484
26 sg:pub.10.1038/sj.gt.3300485
27 https://doi.org/10.1016/0005-2736(92)90316-e
28 https://doi.org/10.1016/0014-5793(94)01315-2
29 https://doi.org/10.1016/0304-4165(81)90342-1
30 https://doi.org/10.1016/s0014-5793(96)01397-x
31 https://doi.org/10.1021/bi952436a
32 https://doi.org/10.1021/bi9602019
33 https://doi.org/10.1021/ja962713g
34 https://doi.org/10.1073/pnas.84.21.7413
35 https://doi.org/10.1073/pnas.90.23.11307
36 https://doi.org/10.1073/pnas.90.7.2812
37 https://doi.org/10.1073/pnas.92.16.7297
38 https://doi.org/10.1073/pnas.93.14.7305
39 https://doi.org/10.1074/jbc.270.32.18997
40 https://doi.org/10.1089/hum.1996.7.14-1701
41 https://doi.org/10.1089/hum.1996.7.15-1791
42 https://doi.org/10.1089/hum.1996.7.15-1803
43 https://doi.org/10.3109/08982109309147441
44 https://doi.org/10.3109/08982109609031138
45 schema:datePublished 1997-09
46 schema:datePublishedReg 1997-09-01
47 schema:description A novel LPD formulation has been developed for in vivo gene transfer. It involves the interaction of plasmid DNA with protamine sulfate, a cationic polypeptide, followed by the addition of DOTAP cationic liposomes. Compared with DOTAP/DNA complexes, LPD offers better protection of plasmid DNA against enzymatic digestion and gives consistently higher gene expression in mice via tail vein injection. When a luciferase reporter gene was employed, gene expression was found in all tissues examined including lung, heart, spleen, liver and kidney with the highest expression in the lung. The in vivo efficiency of LPD was dependent upon charge ratio and was also affected by the lipid used. Increasing the amount of DNA delivered induced an increase in gene expression. The optimal dose was approximately 50 micrograms per mouse at which concentration approximately 20 ng luciferase protein per milligram extracted tissue protein could be detected in the lung. Increasing the DNA to 100 micrograms per mouse resulted in toxicity and death of the animal. Gene expression in the lung was detected as early as 1 h after injection, peaked at 6 h and declined thereafter. High expression was also found in the spleen 6 h after injection but dropped very rapidly thereafter. The in vivo gene expression by LPD was dependent upon the route of administration since intraportal injection of LPD led to about a 100-fold decrease in gene expression in the lung as compared with i.v. injection. Using lacZ as a reporter gene, it was shown that endothelial cells were the primary locus of transgene expression in both the lung and spleen. No sign of inflammation in these organs was noticed. Since protamine sulfate has been proven to be nontoxic and only weakly immunogenic in humans, this novel vector may be useful for clinical gene therapy.
48 schema:genre research_article
49 schema:inLanguage en
50 schema:isAccessibleForFree true
51 schema:isPartOf Nb971b8794a864dbca79acb9a6afae290
52 Ne64dd3a4b4204988b3c173c77a1d955a
53 sg:journal.1105638
54 schema:name In vivo gene transfer via intravenous administration of cationic lipid–protamine–DNA (LPD) complexes
55 schema:pagination 3300482
56 schema:productId N1216278b97a1497b8a04fcb95b41dc70
57 N1e72b51fe7084dcb93cbb6a504c54f13
58 N406d1ddaa7d64ab59985276a6c5bd2f2
59 N4f4665849a354e7e872b07a9cd868885
60 N631822b7bab849a19800713823a55569
61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014791369
62 https://doi.org/10.1038/sj.gt.3300482
63 schema:sdDatePublished 2019-04-11T12:15
64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
65 schema:sdPublisher Ncf5129ea237846dea2e730211d79095f
66 schema:url http://www.nature.com/articles/3300482
67 sgo:license sg:explorer/license/
68 sgo:sdDataset articles
69 rdf:type schema:ScholarlyArticle
70 N023bb701a7314e5aa9681f72070f12f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Lung
72 rdf:type schema:DefinedTerm
73 N09cf94eae98f4e71aa85352503470647 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Cations
75 rdf:type schema:DefinedTerm
76 N1216278b97a1497b8a04fcb95b41dc70 schema:name dimensions_id
77 schema:value pub.1014791369
78 rdf:type schema:PropertyValue
79 N1414c3889a0f4dbda137514bf68d1c22 rdf:first sg:person.01005331112.69
80 rdf:rest rdf:nil
81 N1e72b51fe7084dcb93cbb6a504c54f13 schema:name nlm_unique_id
82 schema:value 9421525
83 rdf:type schema:PropertyValue
84 N381c28c7cbc34d3791f7019c1c78a291 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Animals
86 rdf:type schema:DefinedTerm
87 N3beb10a63ece40c19ad5a166aba6e882 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Fluorescent Dyes
89 rdf:type schema:DefinedTerm
90 N406d1ddaa7d64ab59985276a6c5bd2f2 schema:name pubmed_id
91 schema:value 9349425
92 rdf:type schema:PropertyValue
93 N472afb3b39bd4eb88a4345e915202aa0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name DNA
95 rdf:type schema:DefinedTerm
96 N4f4665849a354e7e872b07a9cd868885 schema:name doi
97 schema:value 10.1038/sj.gt.3300482
98 rdf:type schema:PropertyValue
99 N5458163f5d1747d0abbec36201bd840c rdf:first sg:person.014614151277.22
100 rdf:rest N1414c3889a0f4dbda137514bf68d1c22
101 N631822b7bab849a19800713823a55569 schema:name readcube_id
102 schema:value 46784b1f5153d02f18e38124c180202ca9a70c85a05f355e1c2e8885c128d8d1
103 rdf:type schema:PropertyValue
104 N657b3516262246c8b62aa72b62f85cb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Mice, Inbred Strains
106 rdf:type schema:DefinedTerm
107 N676ba1a90d08404eba9c2ed3c2155ade schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Injections, Intravenous
109 rdf:type schema:DefinedTerm
110 N7bd6e7ef347145bca8e5c2950976e72b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Genetic Vectors
112 rdf:type schema:DefinedTerm
113 N8417fa86a4b2458496357f7d1ca7a563 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Quaternary Ammonium Compounds
115 rdf:type schema:DefinedTerm
116 N87f6e26e70584881b9db2d55331beba1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Gene Transfer Techniques
118 rdf:type schema:DefinedTerm
119 N90419d8e7fb043b8953f80be9ad44f03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Liposomes
121 rdf:type schema:DefinedTerm
122 Nb971b8794a864dbca79acb9a6afae290 schema:volumeNumber 4
123 rdf:type schema:PublicationVolume
124 Nc48bfdfb76414a12a54a2a3792ed10e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Luciferases
126 rdf:type schema:DefinedTerm
127 Ncf5129ea237846dea2e730211d79095f schema:name Springer Nature - SN SciGraph project
128 rdf:type schema:Organization
129 Nd0231b2bef804e288098d61a8a29db16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Mice
131 rdf:type schema:DefinedTerm
132 Nd62c92aecbae4ddaad7139d57ea0705c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Protamines
134 rdf:type schema:DefinedTerm
135 Ne47f9486081d47a99120cd0fe7ff4081 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Female
137 rdf:type schema:DefinedTerm
138 Ne64dd3a4b4204988b3c173c77a1d955a schema:issueNumber 9
139 rdf:type schema:PublicationIssue
140 Nf01cf587da564db78e6d939316649f94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Fatty Acids, Monounsaturated
142 rdf:type schema:DefinedTerm
143 Nf5570019f2a94eef8ad78a406b3cc18e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Gene Expression
145 rdf:type schema:DefinedTerm
146 Nf62e2c32b0b046ac96b2fb05442ec445 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Lac Operon
148 rdf:type schema:DefinedTerm
149 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
150 schema:name Biological Sciences
151 rdf:type schema:DefinedTerm
152 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
153 schema:name Genetics
154 rdf:type schema:DefinedTerm
155 sg:grant.2435629 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3300482
156 rdf:type schema:MonetaryGrant
157 sg:grant.2436124 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3300482
158 rdf:type schema:MonetaryGrant
159 sg:grant.2472090 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.gt.3300482
160 rdf:type schema:MonetaryGrant
161 sg:journal.1105638 schema:issn 0969-7128
162 1476-5462
163 schema:name Gene Therapy
164 rdf:type schema:Periodical
165 sg:person.01005331112.69 schema:familyName Huang
166 schema:givenName L
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005331112.69
168 rdf:type schema:Person
169 sg:person.014614151277.22 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
170 schema:familyName Li
171 schema:givenName S
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014614151277.22
173 rdf:type schema:Person
174 sg:pub.10.1038/357455a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044352249
175 https://doi.org/10.1038/357455a0
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/nbt0297-167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042860966
178 https://doi.org/10.1038/nbt0297-167
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/sj.gt.3300424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031544602
181 https://doi.org/10.1038/sj.gt.3300424
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/sj.gt.3300484 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027651488
184 https://doi.org/10.1038/sj.gt.3300484
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/sj.gt.3300485 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028276876
187 https://doi.org/10.1038/sj.gt.3300485
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/0005-2736(92)90316-e schema:sameAs https://app.dimensions.ai/details/publication/pub.1001002731
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/0014-5793(94)01315-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007571113
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/0304-4165(81)90342-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034092280
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/s0014-5793(96)01397-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007844889
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1021/bi952436a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055211114
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1021/bi9602019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055211657
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1021/ja962713g schema:sameAs https://app.dimensions.ai/details/publication/pub.1055865824
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1073/pnas.84.21.7413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022874269
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1073/pnas.90.23.11307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021985080
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1073/pnas.90.7.2812 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030378744
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1073/pnas.92.16.7297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018266629
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1073/pnas.93.14.7305 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026219069
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1074/jbc.270.32.18997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042873779
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1089/hum.1996.7.14-1701 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271698
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1089/hum.1996.7.15-1791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271707
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1089/hum.1996.7.15-1803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271709
220 rdf:type schema:CreativeWork
221 https://doi.org/10.3109/08982109309147441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001807459
222 rdf:type schema:CreativeWork
223 https://doi.org/10.3109/08982109609031138 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018600370
224 rdf:type schema:CreativeWork
225 https://www.grid.ac/institutes/grid.21925.3d schema:alternateName University of Pittsburgh
226 schema:name Department of Pharmacology, University of Pittsburgh School of Medicine, PA 15261, USA.
227 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...