Replicative retroviral vectors for cancer gene therapy View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-01

AUTHORS

Sounkary K Solly, Stephane Trajcevski, Charlotte Frisén, Georg W Holzer, Elisabeth Nelson, Béatrice Clerc, Evelyn Abordo-Adesida, Maria Castro, Pedro Lowenstein, David Klatzmann

ABSTRACT

Poor efficiency of gene transfer into cancer cells constitutes the major bottleneck of current cancer gene therapy. We reasoned that because tumors are masses of rapidly dividing cells, they would be most efficiently transduced with vector systems allowing transgene propagation. We thus designed two replicative retrovirus-derived vector systems: one inherently replicative vector, and one defective vector propagated by a helper retrovirus. In vitro, both systems achieved very efficient transgene propagation. In immunocompetent mice, replicative vectors transduced >85% tumor cells, whereas defective vectors transduced <1% under similar conditions. It is noteworthy that viral propagation could be efficiently blocked by azido-thymidine, in vitro and in vivo. In a model of established brain tumors treated with suicide genes, replicative retroviral vectors (RRVs) were approximately 1000 times more efficient than defective adenoviral vectors. These results demonstrate the advantage and potential of RRVs and strongly support their development for cancer gene therapy. More... »

PAGES

7700521

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/sj.cgt.7700521

    DOI

    http://dx.doi.org/10.1038/sj.cgt.7700521

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043330274

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12489026


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