Apoptosis-induced cleavage of keratin 15 and keratin 17 in a human breast epithelial cell line View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2001-03

AUTHORS

V Badock, U Steinhusen, K Bommert, B Wittmann-Liebold, A Otto

ABSTRACT

Keratin 15 (K15) and keratin 17 (K17) are intermediate filament (IF) type I proteins that are responsible for the mechanical integrity of epithelial cells. By analyzing the human breast epithelial cell line H184A1 before and after induction of apoptosis by high-resolution two-dimensional gel electrophoresis (2-DE) we identified the caspase-mediated cleavage of keratins 15 and 17. After induction of apoptosis three fragments of both K15 and K17 could be observed by 2 -DE. K15 and K17 proteolysis was observed during staurosporine-induced apoptosis and anoikis (anchorage-dependent apoptosis) as well and was shown to be caspase-dependent. By using mass spectrometry we could determine the caspase cleavage sites, one in K15 and two in K17. The sequence VEMD/A at the cleavage site located in the conserved linker region was found in K15 and K17. A further cleavage site was identified in the tail region of K17 with the recognition motif EVQD/G. More... »

PAGES

4400812

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.cdd.4400812

DOI

http://dx.doi.org/10.1038/sj.cdd.4400812

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035804510

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11319614


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