Surface expression of phosphatidylserine on macrophages is required for phagocytosis of apoptotic thymocytes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-07

AUTHORS

M K Callahan, P Williamson, R A Schlegel

ABSTRACT

Cells generally maintain an asymmetric distribution of phospholipids across the plasma membrane bilayer, restricting the phospholipid, phosphatidylserine (PS), to the inner leaflet of the plasma membrane. When cells undergo apoptosis, this asymmetric transbilayer distribution is lost, bringing PS to the surface where it acts as a signal for engulfment by phagocytes. The fluorescent dye merocyanine 540 specifically stains the plasma membrane of apoptotic cells which have lost their asymmetric distribution of phospholipids. However, it also stains non-apoptotic macrophages, suggesting that phospholipid asymmetry may not be maintained in these cells, and thus that they may express PS on their surface. Here, the PS-binding protein, annexin V, was used to show that in fact normal macrophages do express PS on their surface. Furthermore, pre-treating macrophages with annexin V was found to inhibit phagocytosis of apoptotic thymocytes and thymocytes on which PS expression was artificially induced, but did not inhibit phagocytosis of latex beads or Fc receptor-mediated phagocytosis of opsonized erythrocytes. These results indicate that PS is constitutively expressed on the surface of macrophages and is functionally significant for the phagocytosis of PS-expressing target cells. More... »

PAGES

645

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.cdd.4400690

DOI

http://dx.doi.org/10.1038/sj.cdd.4400690

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011037735

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10889509


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Annexin A5", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dexamethasone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macrophage Activation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macrophages", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Lipids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phagocytosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphatidylserines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pyrimidinones", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Pennsylvania State University", 
          "id": "https://www.grid.ac/institutes/grid.29857.31", 
          "name": [
            "Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16802 USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Callahan", 
        "givenName": "M K", 
        "id": "sg:person.01031067060.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031067060.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Amherst College", 
          "id": "https://www.grid.ac/institutes/grid.252152.3", 
          "name": [
            "Department of Biology, Amherst College, Amherst, MA 01002 USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Williamson", 
        "givenName": "P", 
        "id": "sg:person.0614421072.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614421072.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pennsylvania State University", 
          "id": "https://www.grid.ac/institutes/grid.29857.31", 
          "name": [
            "Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16802 USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schlegel", 
        "givenName": "R A", 
        "id": "sg:person.015016773022.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015016773022.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0005-2736(92)90126-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000858370"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(92)90126-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000858370"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002285862", 
          "https://doi.org/10.1038/sj.cdd.4400491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002285862", 
          "https://doi.org/10.1038/sj.cdd.4400491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4613-1659-6_21", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003421569", 
          "https://doi.org/10.1007/978-1-4613-1659-6_21"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.29.17205", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007614151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0320(19971201)29:4<313::aid-cyto8>3.0.co;2-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007627957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/15690", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010064357", 
          "https://doi.org/10.1038/15690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/15690", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010064357", 
          "https://doi.org/10.1038/15690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci119397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011261559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(76)90241-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013349618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(76)90241-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013349618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400473", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014002330", 
          "https://doi.org/10.1038/sj.cdd.4400473"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400473", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014002330", 
          "https://doi.org/10.1038/sj.cdd.4400473"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.mi.47.100193.001451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015540474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(81)90183-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015951429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(81)90183-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015951429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.29.18240", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017587285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cb.07.110191.003311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025649858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/09687689409162237", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026076802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81201-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029077211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.18.6863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030895347"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.182.5.1597", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031357877"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.42.26159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032553958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(83)90055-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032836196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(83)90055-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032836196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cb.08.110192.000435", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034873421"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(76)90333-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037455048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(76)90333-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037455048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(93)80185-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037957510"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400241", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038235319", 
          "https://doi.org/10.1038/sj.cdd.4400241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400241", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038235319", 
          "https://doi.org/10.1038/sj.cdd.4400241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.8.5.767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039038797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-2478(93)90101-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039738804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-2478(93)90101-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039738804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/09687689409160430", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039962121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1041230209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040005168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1460-9568.1999.00477.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042057201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/33169", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042290529", 
          "https://doi.org/10.1038/33169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/33169", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042290529", 
          "https://doi.org/10.1038/33169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0074-7696(08)62312-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042410192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(74)90175-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045618942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(74)90175-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045618942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.10.3311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048452381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400529", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051289055", 
          "https://doi.org/10.1038/sj.cdd.4400529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4400529", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051289055", 
          "https://doi.org/10.1038/sj.cdd.4400529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(82)90277-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054549932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00314a002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055169784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9606859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9606859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.77.6.1136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063340717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074469654", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075302158", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076942818", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jlb.44.5.337", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079599668"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080009175", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082636332", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082687304", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082794529", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb00353.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082881220"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083354307", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-07", 
    "datePublishedReg": "2000-07-01", 
    "description": "Cells generally maintain an asymmetric distribution of phospholipids across the plasma membrane bilayer, restricting the phospholipid, phosphatidylserine (PS), to the inner leaflet of the plasma membrane. When cells undergo apoptosis, this asymmetric transbilayer distribution is lost, bringing PS to the surface where it acts as a signal for engulfment by phagocytes. The fluorescent dye merocyanine 540 specifically stains the plasma membrane of apoptotic cells which have lost their asymmetric distribution of phospholipids. However, it also stains non-apoptotic macrophages, suggesting that phospholipid asymmetry may not be maintained in these cells, and thus that they may express PS on their surface. Here, the PS-binding protein, annexin V, was used to show that in fact normal macrophages do express PS on their surface. Furthermore, pre-treating macrophages with annexin V was found to inhibit phagocytosis of apoptotic thymocytes and thymocytes on which PS expression was artificially induced, but did not inhibit phagocytosis of latex beads or Fc receptor-mediated phagocytosis of opsonized erythrocytes. These results indicate that PS is constitutively expressed on the surface of macrophages and is functionally significant for the phagocytosis of PS-expressing target cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.cdd.4400690", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1112551", 
        "issn": [
          "1350-9047", 
          "1476-5403"
        ], 
        "name": "Cell Death & Differentiation", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Surface expression of phosphatidylserine on macrophages is required for phagocytosis of apoptotic thymocytes", 
    "pagination": "645", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3496b9cc5962b1d7760a45c45c19fd9b7a693de46fc2759081f79b30481c4427"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10889509"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9437445"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.cdd.4400690"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011037735"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.cdd.4400690", 
      "https://app.dimensions.ai/details/publication/pub.1011037735"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29181_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/4400690"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.cdd.4400690'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.cdd.4400690'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.cdd.4400690'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.cdd.4400690'


 

This table displays all metadata directly associated to this object as RDF triples.

294 TRIPLES      21 PREDICATES      93 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.cdd.4400690 schema:about N37490d8fc5de4f7c81e7b2f0a2548032
2 N4bc3e63131094b1d96ca0626910ee4e6
3 N5896b4b8626a43a987ccfcd581e55fc4
4 N5f9f933e4f7b4cf7a032c6d3725c55d6
5 N6387fa04455c4470afa1968ee8b8a8cb
6 N73fc07edb4b141e0966e3e135abef842
7 N7fd4091294bc4cf8b90674ad95a40210
8 N80a0ea77d09e41489e4ec5f94d44596e
9 Nbbeee1faf911498d950aa732be0b45c4
10 Nbeafd8c070cf44528f5cec605153cf8a
11 Nd2528aedb85047ad9dd6dd14d5a43c0e
12 Nd755c3cd67904e79aa201dc900c992e5
13 Ndd18e59777ad4b68b2bf4e1615310aaf
14 Nf048a584216245af9bd021117fcf6f2a
15 Nf302cfa1e47c4c18b40eb064dbdd15d3
16 Nf44f55e4a9384fb19195ae8115abe37e
17 Nf7577ecd44584fcfa80a8ddaef1e8b25
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author Nda1109024ee04aad9ba78b142afda205
21 schema:citation sg:pub.10.1007/978-1-4613-1659-6_21
22 sg:pub.10.1038/15690
23 sg:pub.10.1038/33169
24 sg:pub.10.1038/sj.cdd.4400241
25 sg:pub.10.1038/sj.cdd.4400473
26 sg:pub.10.1038/sj.cdd.4400491
27 sg:pub.10.1038/sj.cdd.4400529
28 https://app.dimensions.ai/details/publication/pub.1074469654
29 https://app.dimensions.ai/details/publication/pub.1075302158
30 https://app.dimensions.ai/details/publication/pub.1076942818
31 https://app.dimensions.ai/details/publication/pub.1080009175
32 https://app.dimensions.ai/details/publication/pub.1082636332
33 https://app.dimensions.ai/details/publication/pub.1082687304
34 https://app.dimensions.ai/details/publication/pub.1082794529
35 https://app.dimensions.ai/details/publication/pub.1083354307
36 https://doi.org/10.1002/(sici)1097-0320(19971201)29:4<313::aid-cyto8>3.0.co;2-a
37 https://doi.org/10.1002/j.1460-2075.1996.tb00353.x
38 https://doi.org/10.1002/jcp.1041230209
39 https://doi.org/10.1002/jlb.44.5.337
40 https://doi.org/10.1016/0005-2736(74)90175-8
41 https://doi.org/10.1016/0005-2736(76)90241-8
42 https://doi.org/10.1016/0005-2736(76)90333-3
43 https://doi.org/10.1016/0005-2736(83)90055-x
44 https://doi.org/10.1016/0005-2736(92)90126-7
45 https://doi.org/10.1016/0014-5793(93)80185-w
46 https://doi.org/10.1016/0022-1759(81)90183-6
47 https://doi.org/10.1016/0092-8674(82)90277-x
48 https://doi.org/10.1016/0165-2478(93)90101-7
49 https://doi.org/10.1016/s0074-7696(08)62312-8
50 https://doi.org/10.1016/s0092-8674(00)81201-5
51 https://doi.org/10.1021/bi00314a002
52 https://doi.org/10.1021/bi9606859
53 https://doi.org/10.1046/j.1460-9568.1999.00477.x
54 https://doi.org/10.1073/pnas.83.10.3311
55 https://doi.org/10.1073/pnas.83.18.6863
56 https://doi.org/10.1074/jbc.271.29.17205
57 https://doi.org/10.1074/jbc.272.29.18240
58 https://doi.org/10.1074/jbc.272.42.26159
59 https://doi.org/10.1084/jem.182.5.1597
60 https://doi.org/10.1091/mbc.8.5.767
61 https://doi.org/10.1146/annurev.cb.07.110191.003311
62 https://doi.org/10.1146/annurev.cb.08.110192.000435
63 https://doi.org/10.1146/annurev.mi.47.100193.001451
64 https://doi.org/10.1161/01.res.77.6.1136
65 https://doi.org/10.1172/jci119397
66 https://doi.org/10.3109/09687689409160430
67 https://doi.org/10.3109/09687689409162237
68 schema:datePublished 2000-07
69 schema:datePublishedReg 2000-07-01
70 schema:description Cells generally maintain an asymmetric distribution of phospholipids across the plasma membrane bilayer, restricting the phospholipid, phosphatidylserine (PS), to the inner leaflet of the plasma membrane. When cells undergo apoptosis, this asymmetric transbilayer distribution is lost, bringing PS to the surface where it acts as a signal for engulfment by phagocytes. The fluorescent dye merocyanine 540 specifically stains the plasma membrane of apoptotic cells which have lost their asymmetric distribution of phospholipids. However, it also stains non-apoptotic macrophages, suggesting that phospholipid asymmetry may not be maintained in these cells, and thus that they may express PS on their surface. Here, the PS-binding protein, annexin V, was used to show that in fact normal macrophages do express PS on their surface. Furthermore, pre-treating macrophages with annexin V was found to inhibit phagocytosis of apoptotic thymocytes and thymocytes on which PS expression was artificially induced, but did not inhibit phagocytosis of latex beads or Fc receptor-mediated phagocytosis of opsonized erythrocytes. These results indicate that PS is constitutively expressed on the surface of macrophages and is functionally significant for the phagocytosis of PS-expressing target cells.
71 schema:genre research_article
72 schema:inLanguage en
73 schema:isAccessibleForFree true
74 schema:isPartOf N1cd34699bbb74fab9ab44e5580939be1
75 N4adcd450cdfb405293815f62e17ce77b
76 sg:journal.1112551
77 schema:name Surface expression of phosphatidylserine on macrophages is required for phagocytosis of apoptotic thymocytes
78 schema:pagination 645
79 schema:productId N1906e278ff144160877e77e9d1854f4c
80 N6b40c1391af14a46a184eccfde124791
81 N8833525b47574b468f29c8b5ffc7cffe
82 N9adaf90667b7453988a445e13ebcfbf3
83 Nb771fb96d6944f4fa3d71ee927b8fb33
84 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011037735
85 https://doi.org/10.1038/sj.cdd.4400690
86 schema:sdDatePublished 2019-04-11T11:50
87 schema:sdLicense https://scigraph.springernature.com/explorer/license/
88 schema:sdPublisher Nf78fc3f6d289459c800a075e7ac84091
89 schema:url https://www.nature.com/articles/4400690
90 sgo:license sg:explorer/license/
91 sgo:sdDataset articles
92 rdf:type schema:ScholarlyArticle
93 N1906e278ff144160877e77e9d1854f4c schema:name pubmed_id
94 schema:value 10889509
95 rdf:type schema:PropertyValue
96 N1cd34699bbb74fab9ab44e5580939be1 schema:issueNumber 7
97 rdf:type schema:PublicationIssue
98 N37490d8fc5de4f7c81e7b2f0a2548032 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Phagocytosis
100 rdf:type schema:DefinedTerm
101 N4adcd450cdfb405293815f62e17ce77b schema:volumeNumber 7
102 rdf:type schema:PublicationVolume
103 N4bc3e63131094b1d96ca0626910ee4e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Macrophage Activation
105 rdf:type schema:DefinedTerm
106 N5896b4b8626a43a987ccfcd581e55fc4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Calcium
108 rdf:type schema:DefinedTerm
109 N5f9f933e4f7b4cf7a032c6d3725c55d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Mice
111 rdf:type schema:DefinedTerm
112 N5fb2b9c252ea4bc3ac775f7e0e87b9d1 rdf:first sg:person.015016773022.18
113 rdf:rest rdf:nil
114 N6387fa04455c4470afa1968ee8b8a8cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Male
116 rdf:type schema:DefinedTerm
117 N6b40c1391af14a46a184eccfde124791 schema:name nlm_unique_id
118 schema:value 9437445
119 rdf:type schema:PropertyValue
120 N73fc07edb4b141e0966e3e135abef842 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Annexin A5
122 rdf:type schema:DefinedTerm
123 N7fd4091294bc4cf8b90674ad95a40210 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Macrophages
125 rdf:type schema:DefinedTerm
126 N80a0ea77d09e41489e4ec5f94d44596e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Membrane Lipids
128 rdf:type schema:DefinedTerm
129 N8833525b47574b468f29c8b5ffc7cffe schema:name dimensions_id
130 schema:value pub.1011037735
131 rdf:type schema:PropertyValue
132 N9adaf90667b7453988a445e13ebcfbf3 schema:name readcube_id
133 schema:value 3496b9cc5962b1d7760a45c45c19fd9b7a693de46fc2759081f79b30481c4427
134 rdf:type schema:PropertyValue
135 Nb771fb96d6944f4fa3d71ee927b8fb33 schema:name doi
136 schema:value 10.1038/sj.cdd.4400690
137 rdf:type schema:PropertyValue
138 Nbbeee1faf911498d950aa732be0b45c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Phosphatidylserines
140 rdf:type schema:DefinedTerm
141 Nbeafd8c070cf44528f5cec605153cf8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name T-Lymphocytes
143 rdf:type schema:DefinedTerm
144 Nd2528aedb85047ad9dd6dd14d5a43c0e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Pyrimidinones
146 rdf:type schema:DefinedTerm
147 Nd755c3cd67904e79aa201dc900c992e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Animals
149 rdf:type schema:DefinedTerm
150 Nd998bafce3aa4cad9a4f1023d0913fda rdf:first sg:person.0614421072.34
151 rdf:rest N5fb2b9c252ea4bc3ac775f7e0e87b9d1
152 Nda1109024ee04aad9ba78b142afda205 rdf:first sg:person.01031067060.48
153 rdf:rest Nd998bafce3aa4cad9a4f1023d0913fda
154 Ndd18e59777ad4b68b2bf4e1615310aaf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Fluorescent Dyes
156 rdf:type schema:DefinedTerm
157 Nf048a584216245af9bd021117fcf6f2a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Cells, Cultured
159 rdf:type schema:DefinedTerm
160 Nf302cfa1e47c4c18b40eb064dbdd15d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Apoptosis
162 rdf:type schema:DefinedTerm
163 Nf44f55e4a9384fb19195ae8115abe37e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Dexamethasone
165 rdf:type schema:DefinedTerm
166 Nf7577ecd44584fcfa80a8ddaef1e8b25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Glucans
168 rdf:type schema:DefinedTerm
169 Nf78fc3f6d289459c800a075e7ac84091 schema:name Springer Nature - SN SciGraph project
170 rdf:type schema:Organization
171 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
172 schema:name Biological Sciences
173 rdf:type schema:DefinedTerm
174 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
175 schema:name Biochemistry and Cell Biology
176 rdf:type schema:DefinedTerm
177 sg:journal.1112551 schema:issn 1350-9047
178 1476-5403
179 schema:name Cell Death & Differentiation
180 rdf:type schema:Periodical
181 sg:person.01031067060.48 schema:affiliation https://www.grid.ac/institutes/grid.29857.31
182 schema:familyName Callahan
183 schema:givenName M K
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031067060.48
185 rdf:type schema:Person
186 sg:person.015016773022.18 schema:affiliation https://www.grid.ac/institutes/grid.29857.31
187 schema:familyName Schlegel
188 schema:givenName R A
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015016773022.18
190 rdf:type schema:Person
191 sg:person.0614421072.34 schema:affiliation https://www.grid.ac/institutes/grid.252152.3
192 schema:familyName Williamson
193 schema:givenName P
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614421072.34
195 rdf:type schema:Person
196 sg:pub.10.1007/978-1-4613-1659-6_21 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003421569
197 https://doi.org/10.1007/978-1-4613-1659-6_21
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/15690 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010064357
200 https://doi.org/10.1038/15690
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/33169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042290529
203 https://doi.org/10.1038/33169
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/sj.cdd.4400241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038235319
206 https://doi.org/10.1038/sj.cdd.4400241
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/sj.cdd.4400473 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014002330
209 https://doi.org/10.1038/sj.cdd.4400473
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/sj.cdd.4400491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002285862
212 https://doi.org/10.1038/sj.cdd.4400491
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/sj.cdd.4400529 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051289055
215 https://doi.org/10.1038/sj.cdd.4400529
216 rdf:type schema:CreativeWork
217 https://app.dimensions.ai/details/publication/pub.1074469654 schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1075302158 schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1076942818 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1080009175 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1082636332 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1082687304 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1082794529 schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1083354307 schema:CreativeWork
225 https://doi.org/10.1002/(sici)1097-0320(19971201)29:4<313::aid-cyto8>3.0.co;2-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1007627957
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1002/j.1460-2075.1996.tb00353.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082881220
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1002/jcp.1041230209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040005168
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1002/jlb.44.5.337 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079599668
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/0005-2736(74)90175-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045618942
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/0005-2736(76)90241-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013349618
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/0005-2736(76)90333-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037455048
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/0005-2736(83)90055-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032836196
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/0005-2736(92)90126-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000858370
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/0014-5793(93)80185-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1037957510
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/0022-1759(81)90183-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015951429
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/0092-8674(82)90277-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1054549932
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/0165-2478(93)90101-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039738804
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/s0074-7696(08)62312-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042410192
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/s0092-8674(00)81201-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029077211
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1021/bi00314a002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055169784
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1021/bi9606859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055211965
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1046/j.1460-9568.1999.00477.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042057201
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1073/pnas.83.10.3311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048452381
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1073/pnas.83.18.6863 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030895347
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1074/jbc.271.29.17205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007614151
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1074/jbc.272.29.18240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017587285
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1074/jbc.272.42.26159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032553958
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1084/jem.182.5.1597 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031357877
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1091/mbc.8.5.767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039038797
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1146/annurev.cb.07.110191.003311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025649858
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1146/annurev.cb.08.110192.000435 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034873421
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1146/annurev.mi.47.100193.001451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015540474
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1161/01.res.77.6.1136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063340717
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1172/jci119397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011261559
284 rdf:type schema:CreativeWork
285 https://doi.org/10.3109/09687689409160430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039962121
286 rdf:type schema:CreativeWork
287 https://doi.org/10.3109/09687689409162237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026076802
288 rdf:type schema:CreativeWork
289 https://www.grid.ac/institutes/grid.252152.3 schema:alternateName Amherst College
290 schema:name Department of Biology, Amherst College, Amherst, MA 01002 USA
291 rdf:type schema:Organization
292 https://www.grid.ac/institutes/grid.29857.31 schema:alternateName Pennsylvania State University
293 schema:name Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16802 USA
294 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...