Stromal cell-dependent ex vivo expansion of human cord blood progenitors and augmentation of transplantable stem cell activity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-10

AUTHORS

M Kanai, F Hirayama, M Yamaguchi, J Ohkawara, N Sato, K Fukazawa, K Yamashita, M Kuwabara, H Ikeda, K Ikebuchi

ABSTRACT

In vitro maintenance and expansion of human hematopoietic stem cells is crucial for many clinical applications. Thrombopoietin (TPO) and flt3/flk2 ligand (FL) have been suggested to support the proliferation of primitive hematopoietic progenitors and the expansion of transplantable stem cells in culture. In this study, we examined the synergistic effects of the murine stromal cell line MS-5 and a combination of the two cytokines, TPO and FL, on the ex vivo expansion of human cord blood primitive progenitors and transplantable stem cells. A monolayer of MS-5 cells with TPO/FL synergistically supported a more than 600-fold expansion of human cord blood CD34+ cells and CD34+CD38- cells in 2 weeks of culture. Colony-forming unit in culture (CFU-C) and 5-week and 8-week cobblestone area-forming cells (CAFC) were also expanded approximately 300-, 4- and 13-fold, respectively. When MS-5 cells were physically separated from progenitors by a Transwell filter, the synergy was reduced to a quarter of the control, suggesting that direct cell-cell contact between MS-5 cells and progenitors is required for maximum expansion. The severe-combined immunodeficient (scid) mouse-reconstituting cell (SRC) assay demonstrated the slight augmentation of transplantable stem cell activity in culture. These results indicated that MS-5 cells provide a milieu that stimulates the proliferation of primitive progenitors including transplantable stem cells. More... »

PAGES

837

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.bmt.1702634

DOI

http://dx.doi.org/10.1038/sj.bmt.1702634

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050609506

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11081382


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HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.bmt.1702634'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.bmt.1702634'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.bmt.1702634'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.bmt.1702634'


 

This table displays all metadata directly associated to this object as RDF triples.

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323 schema:name TERUMO Corporation, Kanagawa, Japan
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