Analysis of peripheral blood CD34+ cells mobilized with granulocyte colony-stimulating factor (G-CSF) using a long-term culture system View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-04-01

AUTHORS

T Suzuki, K Muroi, Y Amemiya, Y Miura

ABSTRACT

G-CSF administered to healthy volunteers at a dose of 3 μg/kg for 5 days mobilized colony-forming units granulocyte–macrophage (CFU-GM), burst-forming units-erythroid (BFU-E), and long-term culture-initiating cells (LTC-IC) to a maximal level on day 4 or 5. To determine the number of primitive hematopoietic progenitors in the peripheral blood, mononuclear cells (MNCs) or CD34+ cells were cultured in a long-term culture (LTC) system. We defined the colonies produced by cells during LTC at week 2 as differentiated progenitors and those at week 5 as primitive progenitors. G-CSF administered to healthy volunteers increased the number of differentiated progenitors from day 4 and primitive progenitors on days 3–5. Enriched CD34+ cells from healthy volunteers treated with G-CSF (PB-G) or without it (PB-SS), and patients treated with chemotherapy plus G-CSF (PB-CG) were subjected to LTC for 7 weeks. PB CD34+ cells from PB-G contained less primitive progenitors than those from PB-CG. Therefore, in healthy donors administered G-CSF at a dose of 3 μg/kg, the number of PB CD34+ cells should be harvested as much as possible to perform allogeneic peripheral blood stem cell transplantation (PBSCT). More... »

PAGES

751-757

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.bmt.1701162

DOI

http://dx.doi.org/10.1038/sj.bmt.1701162

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034822559

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9603397


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