Polyclonality of BRAF mutations in primary melanoma and the selection of mutant alleles during progression View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-02

AUTHORS

J Lin, Y Goto, H Murata, K Sakaizawa, A Uchiyama, T Saida, M Takata

ABSTRACT

BACKGROUND: Oncogenic BRAF mutation had been considered to be a founder event in the formation of melanocytic tumours; however, we recently argued against this notion by showing marked polyclonality of BRAF mutations in acquired melanocytic nevi (Lin et al, J Natl Cancer Inst., 2009; 101:1423-7). Here, we tested whether similar heterogeneity of BRAF mutations exists in primary melanomas. METHODS: We isolated and sequenced single melanoma cells from five primary melanoma tissues using antibodies against human high-molecular-weight melanoma-associated antigen. We also examined 10 primary melanomas by the sensitive Mutector assay detecting the BRAF(V600E) mutation, as well as by cloning and sequencing of separated alleles. Furthermore, we estimated the frequency of BRAF mutant alleles in paired samples of primary tumour and recurrence or metastasis in three patients. RESULTS: Single-cell mutation analyses revealed that four of five primary melanomas contained both BRAF-wild-type and BRAF-mutant tumour cells. Tumour heterogeneity in terms of BRAF mutations was also shown in 8 of 10 primary melanomas. Selection of BRAF mutant alleles during progression was demonstrated in all the three patients. CONCLUSION: Acquisition of a BRAF mutation is not a founder event, but may be one of the multiple clonal events in melanoma development, which is selected for during the progression. More... »

PAGES

464

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.bjc.6606072

DOI

http://dx.doi.org/10.1038/sj.bjc.6606072

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014125255

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21224857


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alleles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clone Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Melanoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proto-Oncogene Proteins B-raf", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Skin Neoplasms", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Shinshu University", 
          "id": "https://www.grid.ac/institutes/grid.263518.b", 
          "name": [
            "Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lin", 
        "givenName": "J", 
        "id": "sg:person.0770636346.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770636346.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shinshu University", 
          "id": "https://www.grid.ac/institutes/grid.263518.b", 
          "name": [
            "Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Goto", 
        "givenName": "Y", 
        "id": "sg:person.0707152657.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707152657.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shinshu University", 
          "id": "https://www.grid.ac/institutes/grid.263518.b", 
          "name": [
            "Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murata", 
        "givenName": "H", 
        "id": "sg:person.01251524667.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251524667.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shinshu University", 
          "id": "https://www.grid.ac/institutes/grid.263518.b", 
          "name": [
            "Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sakaizawa", 
        "givenName": "K", 
        "id": "sg:person.0641037457.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641037457.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shinshu University", 
          "id": "https://www.grid.ac/institutes/grid.263518.b", 
          "name": [
            "Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Uchiyama", 
        "givenName": "A", 
        "id": "sg:person.0677315031.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677315031.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shinshu University", 
          "id": "https://www.grid.ac/institutes/grid.263518.b", 
          "name": [
            "Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saida", 
        "givenName": "T", 
        "id": "sg:person.012241115471.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012241115471.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Okayama University", 
          "id": "https://www.grid.ac/institutes/grid.261356.5", 
          "name": [
            "Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan", 
            "Department of Dermatology, Okayama University Graduate School of Medical, Dentistry and Pharmaceutical Sciences, Okayama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takata", 
        "givenName": "M", 
        "id": "sg:person.01130542072.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130542072.35"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.ccr.2004.09.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000818705"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03890", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003324113", 
          "https://doi.org/10.1038/nature03890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03890", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003324113", 
          "https://doi.org/10.1038/nature03890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03890", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003324113", 
          "https://doi.org/10.1038/nature03890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-03-3433", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007018612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-03-3433", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007018612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa050092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007050226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa050092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007050226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1208152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012083850", 
          "https://doi.org/10.1038/sj.onc.1208152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1208152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012083850", 
          "https://doi.org/10.1038/sj.onc.1208152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/jid.2008.374", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012674295", 
          "https://doi.org/10.1038/jid.2008.374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1002011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012824919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/djp309", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013117863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-05-2720", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015172163"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05661", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021487923", 
          "https://doi.org/10.1038/nature05661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1541-7786.mcr-07-2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023191843"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00766", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023536940", 
          "https://doi.org/10.1038/nature00766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00766", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023536940", 
          "https://doi.org/10.1038/nature00766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/molehr/gag051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025174830"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bcp.2010.03.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026066898"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.12.032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028079157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00008390-200510000-00011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029245694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00008390-200510000-00011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029245694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00008390-200510000-00011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029245694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.jid.5700366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031670406", 
          "https://doi.org/10.1038/sj.jid.5700366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/jid.2008.30", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032476903", 
          "https://doi.org/10.1038/jid.2008.30"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/humu.20481", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033888478"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035969538", 
          "https://doi.org/10.1038/ng1054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035969538", 
          "https://doi.org/10.1038/ng1054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2006.06.2984", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041185282"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccr.2009.02.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043900550"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/asheducation-2009.1.3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045111761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/asheducation-2009.1.3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045111761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6603283", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052512623", 
          "https://doi.org/10.1038/sj.bjc.6603283"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6603283", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052512623", 
          "https://doi.org/10.1038/sj.bjc.6603283"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0711741105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053628579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1615/critrevimmunol.v24.i4.40", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068131843"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/cc.6.3.3818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072299266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075319073", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076569024", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-02", 
    "datePublishedReg": "2011-02-01", 
    "description": "BACKGROUND: Oncogenic BRAF mutation had been considered to be a founder event in the formation of melanocytic tumours; however, we recently argued against this notion by showing marked polyclonality of BRAF mutations in acquired melanocytic nevi (Lin et al, J Natl Cancer Inst., 2009; 101:1423-7). Here, we tested whether similar heterogeneity of BRAF mutations exists in primary melanomas.\nMETHODS: We isolated and sequenced single melanoma cells from five primary melanoma tissues using antibodies against human high-molecular-weight melanoma-associated antigen. We also examined 10 primary melanomas by the sensitive Mutector assay detecting the BRAF(V600E) mutation, as well as by cloning and sequencing of separated alleles. Furthermore, we estimated the frequency of BRAF mutant alleles in paired samples of primary tumour and recurrence or metastasis in three patients.\nRESULTS: Single-cell mutation analyses revealed that four of five primary melanomas contained both BRAF-wild-type and BRAF-mutant tumour cells. Tumour heterogeneity in terms of BRAF mutations was also shown in 8 of 10 primary melanomas. Selection of BRAF mutant alleles during progression was demonstrated in all the three patients.\nCONCLUSION: Acquisition of a BRAF mutation is not a founder event, but may be one of the multiple clonal events in melanoma development, which is selected for during the progression.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.bjc.6606072", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6001262", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1017082", 
        "issn": [
          "0007-0920", 
          "1532-1827"
        ], 
        "name": "British Journal of Cancer", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "104"
      }
    ], 
    "name": "Polyclonality of BRAF mutations in primary melanoma and the selection of mutant alleles during progression", 
    "pagination": "464", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "434ac6803a611757fe3e7294a7327199e63a21354c4a4aa4dcdd5b2fb609ea94"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21224857"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0370635"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.bjc.6606072"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1014125255"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.bjc.6606072", 
      "https://app.dimensions.ai/details/publication/pub.1014125255"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000436.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/6606072"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6606072'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6606072'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6606072'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6606072'


 

This table displays all metadata directly associated to this object as RDF triples.

243 TRIPLES      21 PREDICATES      66 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.bjc.6606072 schema:about N20344586c3bb42bdbc550447f4a49550
2 N4ba9bcbdcabc4dd58a2ccc6d6f2a8d37
3 N823160a5fcb840609323e8d3bcf58626
4 N901a38c2f06e434ca67fb527d3f3f626
5 Nc1561abd7a8e4ece81b8adbd98ad75a1
6 Nc84eddd5158d4ae0bb87440a10f2a20f
7 Nd8d717a11cb8433494227da57f44d6a4
8 Ne4766206ab6344f489f20375e7dfce1e
9 anzsrc-for:11
10 anzsrc-for:1112
11 schema:author N19b5bc20d9d74c90971ae07006307bea
12 schema:citation sg:pub.10.1038/jid.2008.30
13 sg:pub.10.1038/jid.2008.374
14 sg:pub.10.1038/nature00766
15 sg:pub.10.1038/nature03890
16 sg:pub.10.1038/nature05661
17 sg:pub.10.1038/ng1054
18 sg:pub.10.1038/sj.bjc.6603283
19 sg:pub.10.1038/sj.jid.5700366
20 sg:pub.10.1038/sj.onc.1208152
21 https://app.dimensions.ai/details/publication/pub.1075319073
22 https://app.dimensions.ai/details/publication/pub.1076569024
23 https://doi.org/10.1002/humu.20481
24 https://doi.org/10.1016/j.bcp.2010.03.019
25 https://doi.org/10.1016/j.ccr.2004.09.022
26 https://doi.org/10.1016/j.ccr.2009.02.022
27 https://doi.org/10.1016/j.cell.2007.12.032
28 https://doi.org/10.1056/nejmoa050092
29 https://doi.org/10.1056/nejmoa1002011
30 https://doi.org/10.1073/pnas.0711741105
31 https://doi.org/10.1093/jnci/djp309
32 https://doi.org/10.1093/molehr/gag051
33 https://doi.org/10.1097/00008390-200510000-00011
34 https://doi.org/10.1158/0008-5472.can-03-3433
35 https://doi.org/10.1158/0008-5472.can-05-2720
36 https://doi.org/10.1158/1541-7786.mcr-07-2001
37 https://doi.org/10.1182/asheducation-2009.1.3
38 https://doi.org/10.1200/jco.2006.06.2984
39 https://doi.org/10.1615/critrevimmunol.v24.i4.40
40 https://doi.org/10.4161/cc.6.3.3818
41 schema:datePublished 2011-02
42 schema:datePublishedReg 2011-02-01
43 schema:description BACKGROUND: Oncogenic BRAF mutation had been considered to be a founder event in the formation of melanocytic tumours; however, we recently argued against this notion by showing marked polyclonality of BRAF mutations in acquired melanocytic nevi (Lin et al, J Natl Cancer Inst., 2009; 101:1423-7). Here, we tested whether similar heterogeneity of BRAF mutations exists in primary melanomas. METHODS: We isolated and sequenced single melanoma cells from five primary melanoma tissues using antibodies against human high-molecular-weight melanoma-associated antigen. We also examined 10 primary melanomas by the sensitive Mutector assay detecting the BRAF(V600E) mutation, as well as by cloning and sequencing of separated alleles. Furthermore, we estimated the frequency of BRAF mutant alleles in paired samples of primary tumour and recurrence or metastasis in three patients. RESULTS: Single-cell mutation analyses revealed that four of five primary melanomas contained both BRAF-wild-type and BRAF-mutant tumour cells. Tumour heterogeneity in terms of BRAF mutations was also shown in 8 of 10 primary melanomas. Selection of BRAF mutant alleles during progression was demonstrated in all the three patients. CONCLUSION: Acquisition of a BRAF mutation is not a founder event, but may be one of the multiple clonal events in melanoma development, which is selected for during the progression.
44 schema:genre research_article
45 schema:inLanguage en
46 schema:isAccessibleForFree true
47 schema:isPartOf N3c08683b2a454adfa15a0d240f331074
48 Nbfb5bbeddfaf408ea6af447d43b0d46f
49 sg:journal.1017082
50 schema:name Polyclonality of BRAF mutations in primary melanoma and the selection of mutant alleles during progression
51 schema:pagination 464
52 schema:productId N4e2707569e2f403caeb86920ed66365d
53 N6485dd233bcc4230a49375c5fb787e39
54 N669a1e6795c2407c8e04479d1c5323a3
55 N66dd70a96fcf49f6a3658380f1b448f7
56 Nf218aed4dfc64a9586e93e8b03c8b6d3
57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014125255
58 https://doi.org/10.1038/sj.bjc.6606072
59 schema:sdDatePublished 2019-04-10T18:58
60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
61 schema:sdPublisher Nf5576cdd95534068a848c98577bc7fc5
62 schema:url https://www.nature.com/articles/6606072
63 sgo:license sg:explorer/license/
64 sgo:sdDataset articles
65 rdf:type schema:ScholarlyArticle
66 N0431972dac3d4e618420b4743f23ee9d rdf:first sg:person.01130542072.35
67 rdf:rest rdf:nil
68 N19b5bc20d9d74c90971ae07006307bea rdf:first sg:person.0770636346.77
69 rdf:rest N24784787210d49f889a21c8792597ba3
70 N20344586c3bb42bdbc550447f4a49550 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Skin Neoplasms
72 rdf:type schema:DefinedTerm
73 N24784787210d49f889a21c8792597ba3 rdf:first sg:person.0707152657.01
74 rdf:rest N7f80f6b2e7f74b3fb4aab2ff52b9ef58
75 N2fb53d2e2e464660830436da3ede7fdf rdf:first sg:person.0677315031.36
76 rdf:rest N4496c604f17043f08b3a82eec23a929c
77 N3c08683b2a454adfa15a0d240f331074 schema:issueNumber 3
78 rdf:type schema:PublicationIssue
79 N4496c604f17043f08b3a82eec23a929c rdf:first sg:person.012241115471.74
80 rdf:rest N0431972dac3d4e618420b4743f23ee9d
81 N4ba9bcbdcabc4dd58a2ccc6d6f2a8d37 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Proto-Oncogene Proteins B-raf
83 rdf:type schema:DefinedTerm
84 N4e2707569e2f403caeb86920ed66365d schema:name doi
85 schema:value 10.1038/sj.bjc.6606072
86 rdf:type schema:PropertyValue
87 N61632597ce8a4e88bc823db83227eb52 rdf:first sg:person.0641037457.33
88 rdf:rest N2fb53d2e2e464660830436da3ede7fdf
89 N6485dd233bcc4230a49375c5fb787e39 schema:name nlm_unique_id
90 schema:value 0370635
91 rdf:type schema:PropertyValue
92 N669a1e6795c2407c8e04479d1c5323a3 schema:name readcube_id
93 schema:value 434ac6803a611757fe3e7294a7327199e63a21354c4a4aa4dcdd5b2fb609ea94
94 rdf:type schema:PropertyValue
95 N66dd70a96fcf49f6a3658380f1b448f7 schema:name pubmed_id
96 schema:value 21224857
97 rdf:type schema:PropertyValue
98 N7f80f6b2e7f74b3fb4aab2ff52b9ef58 rdf:first sg:person.01251524667.39
99 rdf:rest N61632597ce8a4e88bc823db83227eb52
100 N823160a5fcb840609323e8d3bcf58626 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Cell Line, Tumor
102 rdf:type schema:DefinedTerm
103 N901a38c2f06e434ca67fb527d3f3f626 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Mutation
105 rdf:type schema:DefinedTerm
106 Nbfb5bbeddfaf408ea6af447d43b0d46f schema:volumeNumber 104
107 rdf:type schema:PublicationVolume
108 Nc1561abd7a8e4ece81b8adbd98ad75a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Clone Cells
110 rdf:type schema:DefinedTerm
111 Nc84eddd5158d4ae0bb87440a10f2a20f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Humans
113 rdf:type schema:DefinedTerm
114 Nd8d717a11cb8433494227da57f44d6a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Alleles
116 rdf:type schema:DefinedTerm
117 Ne4766206ab6344f489f20375e7dfce1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Melanoma
119 rdf:type schema:DefinedTerm
120 Nf218aed4dfc64a9586e93e8b03c8b6d3 schema:name dimensions_id
121 schema:value pub.1014125255
122 rdf:type schema:PropertyValue
123 Nf5576cdd95534068a848c98577bc7fc5 schema:name Springer Nature - SN SciGraph project
124 rdf:type schema:Organization
125 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
126 schema:name Medical and Health Sciences
127 rdf:type schema:DefinedTerm
128 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
129 schema:name Oncology and Carcinogenesis
130 rdf:type schema:DefinedTerm
131 sg:grant.6001262 http://pending.schema.org/fundedItem sg:pub.10.1038/sj.bjc.6606072
132 rdf:type schema:MonetaryGrant
133 sg:journal.1017082 schema:issn 0007-0920
134 1532-1827
135 schema:name British Journal of Cancer
136 rdf:type schema:Periodical
137 sg:person.01130542072.35 schema:affiliation https://www.grid.ac/institutes/grid.261356.5
138 schema:familyName Takata
139 schema:givenName M
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130542072.35
141 rdf:type schema:Person
142 sg:person.012241115471.74 schema:affiliation https://www.grid.ac/institutes/grid.263518.b
143 schema:familyName Saida
144 schema:givenName T
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012241115471.74
146 rdf:type schema:Person
147 sg:person.01251524667.39 schema:affiliation https://www.grid.ac/institutes/grid.263518.b
148 schema:familyName Murata
149 schema:givenName H
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251524667.39
151 rdf:type schema:Person
152 sg:person.0641037457.33 schema:affiliation https://www.grid.ac/institutes/grid.263518.b
153 schema:familyName Sakaizawa
154 schema:givenName K
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641037457.33
156 rdf:type schema:Person
157 sg:person.0677315031.36 schema:affiliation https://www.grid.ac/institutes/grid.263518.b
158 schema:familyName Uchiyama
159 schema:givenName A
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677315031.36
161 rdf:type schema:Person
162 sg:person.0707152657.01 schema:affiliation https://www.grid.ac/institutes/grid.263518.b
163 schema:familyName Goto
164 schema:givenName Y
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707152657.01
166 rdf:type schema:Person
167 sg:person.0770636346.77 schema:affiliation https://www.grid.ac/institutes/grid.263518.b
168 schema:familyName Lin
169 schema:givenName J
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770636346.77
171 rdf:type schema:Person
172 sg:pub.10.1038/jid.2008.30 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032476903
173 https://doi.org/10.1038/jid.2008.30
174 rdf:type schema:CreativeWork
175 sg:pub.10.1038/jid.2008.374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012674295
176 https://doi.org/10.1038/jid.2008.374
177 rdf:type schema:CreativeWork
178 sg:pub.10.1038/nature00766 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023536940
179 https://doi.org/10.1038/nature00766
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/nature03890 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003324113
182 https://doi.org/10.1038/nature03890
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/nature05661 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021487923
185 https://doi.org/10.1038/nature05661
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/ng1054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035969538
188 https://doi.org/10.1038/ng1054
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/sj.bjc.6603283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052512623
191 https://doi.org/10.1038/sj.bjc.6603283
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/sj.jid.5700366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031670406
194 https://doi.org/10.1038/sj.jid.5700366
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/sj.onc.1208152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012083850
197 https://doi.org/10.1038/sj.onc.1208152
198 rdf:type schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1075319073 schema:CreativeWork
200 https://app.dimensions.ai/details/publication/pub.1076569024 schema:CreativeWork
201 https://doi.org/10.1002/humu.20481 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033888478
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.bcp.2010.03.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026066898
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/j.ccr.2004.09.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000818705
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.ccr.2009.02.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043900550
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/j.cell.2007.12.032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028079157
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1056/nejmoa050092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007050226
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1056/nejmoa1002011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012824919
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1073/pnas.0711741105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053628579
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1093/jnci/djp309 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013117863
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1093/molehr/gag051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025174830
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1097/00008390-200510000-00011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029245694
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1158/0008-5472.can-03-3433 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007018612
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1158/0008-5472.can-05-2720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015172163
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1158/1541-7786.mcr-07-2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023191843
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1182/asheducation-2009.1.3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045111761
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1200/jco.2006.06.2984 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041185282
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1615/critrevimmunol.v24.i4.40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068131843
234 rdf:type schema:CreativeWork
235 https://doi.org/10.4161/cc.6.3.3818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072299266
236 rdf:type schema:CreativeWork
237 https://www.grid.ac/institutes/grid.261356.5 schema:alternateName Okayama University
238 schema:name Department of Dermatology, Okayama University Graduate School of Medical, Dentistry and Pharmaceutical Sciences, Okayama, Japan
239 Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan
240 rdf:type schema:Organization
241 https://www.grid.ac/institutes/grid.263518.b schema:alternateName Shinshu University
242 schema:name Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan
243 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...