Fusion of HIV-1 Tat protein transduction domain to poly-lysine as a new DNA delivery tool View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2004-03

AUTHORS

H Hashida, M Miyamoto, Y Cho, Y Hida, K Kato, T Kurokawa, S Okushiba, S Kondo, H Dosaka-Akita, H Katoh

ABSTRACT

Effective gene therapy depends on the efficient transfer of therapeutic genes to target cells. None of the current technologies, however, satisfy all of the requirements necessary for gene therapy, because the plasma and nuclear membranes of mammalian cells are tight barriers against gene transfer using synthetic delivery systems. The protein transduction domain (PTD) of human immunodeficiency virus type 1 (HIV-1) Tat protein greatly facilitates protein transfer via membrane destabilisation. We synthesised polylysine peptides containing Tat PTD (TAT-pK), or other sequences, and investigated their potential as agents for gene transfer. The synthesised polypeptide TAT-pK retains DNA binding function and mediates delivery of a reporter gene to cultured cells. RGD motif binds with low affinity to alpha integrins which induce cell activation. Two control polypeptides, GGG-pK and RGD-pK, were synthesised and tested, but their gene transfer abilities were weaker than those of TAT-pK. TAT-pK-mediated gene transfer was enhanced in the presence of chloroquine or ammonium chloride, to a greater extent than that of cationic lipid-mediated gene transfer in most cancer cell lines tested. These data suggest that TAT-pK may be a potent candidate delivery vehicle that promotes gene transfer, dependent on the endocytic pathway. We conclude that the TAT-pK/DNA complex is useful as a minimal unit to package therapeutic genes and to transduce them into mammalian cells. More... »

PAGES

6601680

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.bjc.6601680

DOI

http://dx.doi.org/10.1038/sj.bjc.6601680

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020847439

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15026809


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma, Squamous Cell", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Esophageal Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Products, tat", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lung Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pancreatic Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Fragments", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polylysine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "tat Gene Products, Human Immunodeficiency Virus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hashida", 
        "givenName": "H", 
        "id": "sg:person.01113660553.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113660553.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miyamoto", 
        "givenName": "M", 
        "id": "sg:person.0637055475.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637055475.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cho", 
        "givenName": "Y", 
        "id": "sg:person.0707172564.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707172564.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hida", 
        "givenName": "Y", 
        "id": "sg:person.0660231513.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660231513.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kato", 
        "givenName": "K", 
        "id": "sg:person.01022342621.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022342621.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kurokawa", 
        "givenName": "T", 
        "id": "sg:person.0576244550.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576244550.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Okushiba", 
        "givenName": "S", 
        "id": "sg:person.014526003704.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014526003704.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kondo", 
        "givenName": "S", 
        "id": "sg:person.01264520473.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264520473.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Medical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, Hokkaido, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dosaka-Akita", 
        "givenName": "H", 
        "id": "sg:person.0711114730.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0711114730.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hokkaido University", 
          "id": "https://www.grid.ac/institutes/grid.39158.36", 
          "name": [
            "Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Katoh", 
        "givenName": "H", 
        "id": "sg:person.01224255263.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224255263.13"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1074/jbc.m110017200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000986116"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.12.5731", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003641933"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90231-e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007312497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/11.5.1295", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008741303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m007540200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013690796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1994.tb20049.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020807756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.10.4255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022455483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m010625200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022867699"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.285.5433.1569", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024331241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.1996.0088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026273088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/4042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026821428", 
          "https://doi.org/10.1038/4042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/4042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026821428", 
          "https://doi.org/10.1038/4042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/5.7.913", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027674288"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/82199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030025933", 
          "https://doi.org/10.1038/82199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.22.12816", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031426128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.97.26.14772", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034816993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035201529", 
          "https://doi.org/10.1038/sj.gt.3301056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035201529", 
          "https://doi.org/10.1038/sj.gt.3301056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.11.4033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043296141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.30.18188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043402039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81843-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048415508"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.25.16010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049706868"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1996.7.15-1907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1997.8.5-511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059272021"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb07697.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078166872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082671798", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2004-03", 
    "datePublishedReg": "2004-03-01", 
    "description": "Effective gene therapy depends on the efficient transfer of therapeutic genes to target cells. None of the current technologies, however, satisfy all of the requirements necessary for gene therapy, because the plasma and nuclear membranes of mammalian cells are tight barriers against gene transfer using synthetic delivery systems. The protein transduction domain (PTD) of human immunodeficiency virus type 1 (HIV-1) Tat protein greatly facilitates protein transfer via membrane destabilisation. We synthesised polylysine peptides containing Tat PTD (TAT-pK), or other sequences, and investigated their potential as agents for gene transfer. The synthesised polypeptide TAT-pK retains DNA binding function and mediates delivery of a reporter gene to cultured cells. RGD motif binds with low affinity to alpha integrins which induce cell activation. Two control polypeptides, GGG-pK and RGD-pK, were synthesised and tested, but their gene transfer abilities were weaker than those of TAT-pK. TAT-pK-mediated gene transfer was enhanced in the presence of chloroquine or ammonium chloride, to a greater extent than that of cationic lipid-mediated gene transfer in most cancer cell lines tested. These data suggest that TAT-pK may be a potent candidate delivery vehicle that promotes gene transfer, dependent on the endocytic pathway. We conclude that the TAT-pK/DNA complex is useful as a minimal unit to package therapeutic genes and to transduce them into mammalian cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.bjc.6601680", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1017082", 
        "issn": [
          "0007-0920", 
          "1532-1827"
        ], 
        "name": "British Journal of Cancer", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "90"
      }
    ], 
    "name": "Fusion of HIV-1 Tat protein transduction domain to poly-lysine as a new DNA delivery tool", 
    "pagination": "6601680", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "6354643c21a51890318dc6697d8a4c2b9028893dbcee167807e00d0753bb5b76"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15026809"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0370635"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.bjc.6601680"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1020847439"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.bjc.6601680", 
      "https://app.dimensions.ai/details/publication/pub.1020847439"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29207_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/6601680"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6601680'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6601680'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6601680'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6601680'


 

This table displays all metadata directly associated to this object as RDF triples.

271 TRIPLES      21 PREDICATES      69 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.bjc.6601680 schema:about N00b7869e61ab40ebab3dca8f6eef9143
2 N01b7bc5299f34d93a9b5dd1056877feb
3 N1f476123fe8647d78e23f32beff41b9e
4 N398d8f5f35564ce5a733723f7d9922b6
5 N415c263d5f4d4da49c6c9104022fa2ef
6 N4353b461b98642b59781ace99399ecdd
7 N5756d5298e96472899842a49f722c44f
8 N5e36288c2dc747818076d52696eb472e
9 N87d9c9b7a63543d8adfd53b98e57d004
10 N8bf9f7fb825a493aaa6414caef549f0b
11 Na4e4e7b756064ca7a33632b682ff4658
12 Nba41a25d6b17429bafda6bab9ea4c60a
13 Ncf8306063aeb4c9e8bdf399f5004e17f
14 Nd8c2f122dd6446f0930a1b3b2de374c0
15 Nde3d1cad9ffc4b4784d9051f38978037
16 Ne17c30d30a9a47e594db34f096c09d4b
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author Nabb267cfd21d4819bff35e135dbf4ab1
20 schema:citation sg:pub.10.1038/4042
21 sg:pub.10.1038/82199
22 sg:pub.10.1038/sj.gt.3301056
23 https://app.dimensions.ai/details/publication/pub.1082671798
24 https://doi.org/10.1002/j.1460-2075.1991.tb07697.x
25 https://doi.org/10.1006/viro.1996.0088
26 https://doi.org/10.1016/0092-8674(93)90231-e
27 https://doi.org/10.1016/s0092-8674(00)81843-7
28 https://doi.org/10.1073/pnas.87.11.4033
29 https://doi.org/10.1073/pnas.88.10.4255
30 https://doi.org/10.1073/pnas.93.12.5731
31 https://doi.org/10.1073/pnas.96.22.12816
32 https://doi.org/10.1073/pnas.97.26.14772
33 https://doi.org/10.1074/jbc.271.30.18188
34 https://doi.org/10.1074/jbc.272.25.16010
35 https://doi.org/10.1074/jbc.m007540200
36 https://doi.org/10.1074/jbc.m010625200
37 https://doi.org/10.1074/jbc.m110017200
38 https://doi.org/10.1089/hum.1996.7.15-1907
39 https://doi.org/10.1089/hum.1997.8.5-511
40 https://doi.org/10.1093/hmg/5.7.913
41 https://doi.org/10.1093/nar/11.5.1295
42 https://doi.org/10.1111/j.1432-1033.1994.tb20049.x
43 https://doi.org/10.1126/science.285.5433.1569
44 schema:datePublished 2004-03
45 schema:datePublishedReg 2004-03-01
46 schema:description Effective gene therapy depends on the efficient transfer of therapeutic genes to target cells. None of the current technologies, however, satisfy all of the requirements necessary for gene therapy, because the plasma and nuclear membranes of mammalian cells are tight barriers against gene transfer using synthetic delivery systems. The protein transduction domain (PTD) of human immunodeficiency virus type 1 (HIV-1) Tat protein greatly facilitates protein transfer via membrane destabilisation. We synthesised polylysine peptides containing Tat PTD (TAT-pK), or other sequences, and investigated their potential as agents for gene transfer. The synthesised polypeptide TAT-pK retains DNA binding function and mediates delivery of a reporter gene to cultured cells. RGD motif binds with low affinity to alpha integrins which induce cell activation. Two control polypeptides, GGG-pK and RGD-pK, were synthesised and tested, but their gene transfer abilities were weaker than those of TAT-pK. TAT-pK-mediated gene transfer was enhanced in the presence of chloroquine or ammonium chloride, to a greater extent than that of cationic lipid-mediated gene transfer in most cancer cell lines tested. These data suggest that TAT-pK may be a potent candidate delivery vehicle that promotes gene transfer, dependent on the endocytic pathway. We conclude that the TAT-pK/DNA complex is useful as a minimal unit to package therapeutic genes and to transduce them into mammalian cells.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree true
50 schema:isPartOf N023559e6d27d464f94b984205f61386b
51 N629bf90238e44ce6896fa0b32afdfd02
52 sg:journal.1017082
53 schema:name Fusion of HIV-1 Tat protein transduction domain to poly-lysine as a new DNA delivery tool
54 schema:pagination 6601680
55 schema:productId N135d40e17084471ba1cfbe68523d94f0
56 N3183dc028864433d90b6d1ce462c5b79
57 N419019ab2afe4b29a27ace4f7709c5d9
58 Ndb6f90e24c264631b86a01815937d072
59 Ne52e531e3e3d4be0bfad59fe73c1e304
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020847439
61 https://doi.org/10.1038/sj.bjc.6601680
62 schema:sdDatePublished 2019-04-11T11:55
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher N0ece5d11bf374f05b4d592dfdede2592
65 schema:url http://www.nature.com/articles/6601680
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N00b7869e61ab40ebab3dca8f6eef9143 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Gene Transfer Techniques
71 rdf:type schema:DefinedTerm
72 N01b7bc5299f34d93a9b5dd1056877feb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name tat Gene Products, Human Immunodeficiency Virus
74 rdf:type schema:DefinedTerm
75 N023559e6d27d464f94b984205f61386b schema:volumeNumber 90
76 rdf:type schema:PublicationVolume
77 N0de5fbe9398a4762a8a2aa15aa8e16d7 rdf:first sg:person.0660231513.46
78 rdf:rest N52c7a390711b4828a6d161254a45a06d
79 N0ece5d11bf374f05b4d592dfdede2592 schema:name Springer Nature - SN SciGraph project
80 rdf:type schema:Organization
81 N135d40e17084471ba1cfbe68523d94f0 schema:name dimensions_id
82 schema:value pub.1020847439
83 rdf:type schema:PropertyValue
84 N1f476123fe8647d78e23f32beff41b9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Tumor Cells, Cultured
86 rdf:type schema:DefinedTerm
87 N3183dc028864433d90b6d1ce462c5b79 schema:name readcube_id
88 schema:value 6354643c21a51890318dc6697d8a4c2b9028893dbcee167807e00d0753bb5b76
89 rdf:type schema:PropertyValue
90 N398d8f5f35564ce5a733723f7d9922b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Genes, Reporter
92 rdf:type schema:DefinedTerm
93 N415c263d5f4d4da49c6c9104022fa2ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Pancreatic Neoplasms
95 rdf:type schema:DefinedTerm
96 N419019ab2afe4b29a27ace4f7709c5d9 schema:name nlm_unique_id
97 schema:value 0370635
98 rdf:type schema:PropertyValue
99 N4353b461b98642b59781ace99399ecdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Carcinoma, Squamous Cell
101 rdf:type schema:DefinedTerm
102 N49c8152bbbc34fa287e0436897abf09e rdf:first sg:person.0711114730.26
103 rdf:rest N4c528fbd422f426bb6e9544966d0405d
104 N4c528fbd422f426bb6e9544966d0405d rdf:first sg:person.01224255263.13
105 rdf:rest rdf:nil
106 N52c7a390711b4828a6d161254a45a06d rdf:first sg:person.01022342621.32
107 rdf:rest Naf2ada899b3944f89cc8861dde33ade3
108 N5756d5298e96472899842a49f722c44f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Carcinoma
110 rdf:type schema:DefinedTerm
111 N5e36288c2dc747818076d52696eb472e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Cell Line
113 rdf:type schema:DefinedTerm
114 N629bf90238e44ce6896fa0b32afdfd02 schema:issueNumber 6
115 rdf:type schema:PublicationIssue
116 N87d9c9b7a63543d8adfd53b98e57d004 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Humans
118 rdf:type schema:DefinedTerm
119 N8bf9f7fb825a493aaa6414caef549f0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Esophageal Neoplasms
121 rdf:type schema:DefinedTerm
122 Na4e4e7b756064ca7a33632b682ff4658 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Recombinant Fusion Proteins
124 rdf:type schema:DefinedTerm
125 Nabb267cfd21d4819bff35e135dbf4ab1 rdf:first sg:person.01113660553.98
126 rdf:rest Nae1d2d6b46c548ebb763fb67b989a118
127 Nae1d2d6b46c548ebb763fb67b989a118 rdf:first sg:person.0637055475.74
128 rdf:rest Nbc24678cf16042ab8f984a6a227ad859
129 Naf2ada899b3944f89cc8861dde33ade3 rdf:first sg:person.0576244550.22
130 rdf:rest Nbb53315745da43adbf83cdd0fb7a7db5
131 Nba41a25d6b17429bafda6bab9ea4c60a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Genetic Therapy
133 rdf:type schema:DefinedTerm
134 Nbb53315745da43adbf83cdd0fb7a7db5 rdf:first sg:person.014526003704.33
135 rdf:rest Ne5cf18f55af5409abf38f00461e0084d
136 Nbc24678cf16042ab8f984a6a227ad859 rdf:first sg:person.0707172564.07
137 rdf:rest N0de5fbe9398a4762a8a2aa15aa8e16d7
138 Ncf8306063aeb4c9e8bdf399f5004e17f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Gene Products, tat
140 rdf:type schema:DefinedTerm
141 Nd8c2f122dd6446f0930a1b3b2de374c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Peptide Fragments
143 rdf:type schema:DefinedTerm
144 Ndb6f90e24c264631b86a01815937d072 schema:name doi
145 schema:value 10.1038/sj.bjc.6601680
146 rdf:type schema:PropertyValue
147 Nde3d1cad9ffc4b4784d9051f38978037 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Lung Neoplasms
149 rdf:type schema:DefinedTerm
150 Ne17c30d30a9a47e594db34f096c09d4b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Polylysine
152 rdf:type schema:DefinedTerm
153 Ne52e531e3e3d4be0bfad59fe73c1e304 schema:name pubmed_id
154 schema:value 15026809
155 rdf:type schema:PropertyValue
156 Ne5cf18f55af5409abf38f00461e0084d rdf:first sg:person.01264520473.17
157 rdf:rest N49c8152bbbc34fa287e0436897abf09e
158 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
159 schema:name Biological Sciences
160 rdf:type schema:DefinedTerm
161 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
162 schema:name Biochemistry and Cell Biology
163 rdf:type schema:DefinedTerm
164 sg:journal.1017082 schema:issn 0007-0920
165 1532-1827
166 schema:name British Journal of Cancer
167 rdf:type schema:Periodical
168 sg:person.01022342621.32 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
169 schema:familyName Kato
170 schema:givenName K
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022342621.32
172 rdf:type schema:Person
173 sg:person.01113660553.98 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
174 schema:familyName Hashida
175 schema:givenName H
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113660553.98
177 rdf:type schema:Person
178 sg:person.01224255263.13 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
179 schema:familyName Katoh
180 schema:givenName H
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224255263.13
182 rdf:type schema:Person
183 sg:person.01264520473.17 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
184 schema:familyName Kondo
185 schema:givenName S
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264520473.17
187 rdf:type schema:Person
188 sg:person.014526003704.33 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
189 schema:familyName Okushiba
190 schema:givenName S
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014526003704.33
192 rdf:type schema:Person
193 sg:person.0576244550.22 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
194 schema:familyName Kurokawa
195 schema:givenName T
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576244550.22
197 rdf:type schema:Person
198 sg:person.0637055475.74 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
199 schema:familyName Miyamoto
200 schema:givenName M
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637055475.74
202 rdf:type schema:Person
203 sg:person.0660231513.46 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
204 schema:familyName Hida
205 schema:givenName Y
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660231513.46
207 rdf:type schema:Person
208 sg:person.0707172564.07 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
209 schema:familyName Cho
210 schema:givenName Y
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707172564.07
212 rdf:type schema:Person
213 sg:person.0711114730.26 schema:affiliation https://www.grid.ac/institutes/grid.39158.36
214 schema:familyName Dosaka-Akita
215 schema:givenName H
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0711114730.26
217 rdf:type schema:Person
218 sg:pub.10.1038/4042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026821428
219 https://doi.org/10.1038/4042
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/82199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030025933
222 https://doi.org/10.1038/82199
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/sj.gt.3301056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035201529
225 https://doi.org/10.1038/sj.gt.3301056
226 rdf:type schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1082671798 schema:CreativeWork
228 https://doi.org/10.1002/j.1460-2075.1991.tb07697.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078166872
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1006/viro.1996.0088 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026273088
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/0092-8674(93)90231-e schema:sameAs https://app.dimensions.ai/details/publication/pub.1007312497
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/s0092-8674(00)81843-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048415508
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1073/pnas.87.11.4033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043296141
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1073/pnas.88.10.4255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022455483
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1073/pnas.93.12.5731 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003641933
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1073/pnas.96.22.12816 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031426128
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1073/pnas.97.26.14772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034816993
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1074/jbc.271.30.18188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043402039
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1074/jbc.272.25.16010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049706868
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1074/jbc.m007540200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013690796
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1074/jbc.m010625200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022867699
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1074/jbc.m110017200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000986116
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1089/hum.1996.7.15-1907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271719
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1089/hum.1997.8.5-511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272021
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1093/hmg/5.7.913 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027674288
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1093/nar/11.5.1295 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008741303
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1111/j.1432-1033.1994.tb20049.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020807756
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1126/science.285.5433.1569 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024331241
267 rdf:type schema:CreativeWork
268 https://www.grid.ac/institutes/grid.39158.36 schema:alternateName Hokkaido University
269 schema:name Department of Medical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, Hokkaido, Japan
270 Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan
271 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...