Agreement between chromogenic in situ hybridisation (CISH) and FISH in the determination of HER2 status in breast cancer View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-05

AUTHORS

L Arnould, Y Denoux, G MacGrogan, F Penault-Llorca, M Fiche, I Treilleux, M C Mathieu, A Vincent-Salomon, M O Vilain, J Couturier

ABSTRACT

Determination of the HER2/neu (HER2) status in breast carcinoma has become necessary for the selection of breast cancer patients for trastuzumab therapy. Amplification of the gene analysed by fluorescence in situ hybridisation (FISH) or overexpression of the protein determined by immunohistochemistry (IHC) are the two major methods to establish this status. A strong correlation has been previously demonstrated between these two methods. However, FISH is not always feasible in routine practice and weakly positive IHC tumours (2+) do not always correspond to a gene amplification. Our study was performed in order to evaluate the contribution of chromogenic in situ hybridisation (CISH), which enables detection of the gene copies through an immunoperoxidase reaction. CISH was performed in 79 breast carcinomas for which the HER2 status was previously determined by IHC and FISH. The results of IHC, FISH and CISH were compared for each tumour. CISH procedures were successful in 95% of our cases. Whatever the IHC results, we found a very good concordance (96%) between CISH and FISH. Our study confirms that CISH may be an alternative to FISH for the determination of the gene amplification status in 2+ tumours. Our results allow us to think that, in many laboratories, CISH may also be an excellent method to calibrate the IHC procedures or, as a quality control test, to check regularly that the IHC signal is in agreement with the gene status. More... »

PAGES

6600943

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/sj.bjc.6600943

DOI

http://dx.doi.org/10.1038/sj.bjc.6600943

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036715126

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12771927


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Breast Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Amplification", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, erbB-2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunohistochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sensitivity and Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Centre Georges Fran\u00e7ois Leclerc", 
          "id": "https://www.grid.ac/institutes/grid.418037.9", 
          "name": [
            "Department of Pathology, Centre GF Leclerc, 1 rue Pr Marion, 21034 Dijon cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arnould", 
        "givenName": "L", 
        "id": "sg:person.01121675220.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121675220.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UniCancer Group", 
          "id": "https://www.grid.ac/institutes/grid.418189.d", 
          "name": [
            "Department of Pathology, Centre F Baclesse, Caen, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Denoux", 
        "givenName": "Y", 
        "id": "sg:person.01021320600.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021320600.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institut Bergognie, Bordeaux, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "MacGrogan", 
        "givenName": "G", 
        "id": "sg:person.01253370320.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253370320.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Centre J Perrin, Clermont-Ferrand, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Penault-Llorca", 
        "givenName": "F", 
        "id": "sg:person.0643603315.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643603315.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centre Hospitalier Universitaire de Nantes", 
          "id": "https://www.grid.ac/institutes/grid.277151.7", 
          "name": [
            "H\u00f4pital G et R Laennec, Nantes, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fiche", 
        "givenName": "M", 
        "id": "sg:person.0706637557.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706637557.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centre L\u00e9on B\u00e9rard", 
          "id": "https://www.grid.ac/institutes/grid.418116.b", 
          "name": [
            "Centre L Berard, Lyon, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Treilleux", 
        "givenName": "I", 
        "id": "sg:person.01210131470.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210131470.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Gustave Roussy", 
          "id": "https://www.grid.ac/institutes/grid.14925.3b", 
          "name": [
            "Institut G Roussy, Villejuif, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mathieu", 
        "givenName": "M C", 
        "id": "sg:person.012211445072.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012211445072.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute Curie", 
          "id": "https://www.grid.ac/institutes/grid.418596.7", 
          "name": [
            "Institut Curie, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vincent-Salomon", 
        "givenName": "A", 
        "id": "sg:person.01306222052.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306222052.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Centre O Lambret, Lille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vilain", 
        "givenName": "M O", 
        "id": "sg:person.01317343552.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317343552.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute Curie", 
          "id": "https://www.grid.ac/institutes/grid.418596.7", 
          "name": [
            "Institut Curie, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Couturier", 
        "givenName": "J", 
        "id": "sg:person.0760136540.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760136540.00"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/jnci/94.11.852", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010730561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.12.5321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011077656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011441671", 
          "https://doi.org/10.1038/modpathol.3880007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011441671", 
          "https://doi.org/10.1038/modpathol.3880007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1309/vacp-vlqa-g9dx-vudf", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014241811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/94.11.855", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015062444"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.971", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017034982"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)64785-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023575302"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880582", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030675311", 
          "https://doi.org/10.1038/modpathol.3880582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880582", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030675311", 
          "https://doi.org/10.1038/modpathol.3880582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm200103153441101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031768583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032076676", 
          "https://doi.org/10.1038/modpathol.3880228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032076676", 
          "https://doi.org/10.1038/modpathol.3880228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033626551", 
          "https://doi.org/10.1038/modpathol.3880154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033626551", 
          "https://doi.org/10.1038/modpathol.3880154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2559.2003.01598.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034591528"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/annonc/12.suppl_1.s101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044332874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1309/980m-e24r-v19k-595d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044347783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0093-7754(01)90281-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049670670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1309/aj84-50ak-1x1b-1q4c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053290441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/sonc.2001.28549", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057078448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.20.3.719", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064202789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.1999.17.1.434", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074511260"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.1999.17.7.1974", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074549797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.1999.17.9.2639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074549894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074665592", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2000.18.21.3651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074719576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2001.19.2.354", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074768320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2001.19.10.2714", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074814016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/107327480100800504", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074887583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/107327480100800504", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074887583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074889449", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082561095", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083122347", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.1998.16.8.2659", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083305984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2002.20.3.719", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083941918"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-05", 
    "datePublishedReg": "2003-05-01", 
    "description": "Determination of the HER2/neu (HER2) status in breast carcinoma has become necessary for the selection of breast cancer patients for trastuzumab therapy. Amplification of the gene analysed by fluorescence in situ hybridisation (FISH) or overexpression of the protein determined by immunohistochemistry (IHC) are the two major methods to establish this status. A strong correlation has been previously demonstrated between these two methods. However, FISH is not always feasible in routine practice and weakly positive IHC tumours (2+) do not always correspond to a gene amplification. Our study was performed in order to evaluate the contribution of chromogenic in situ hybridisation (CISH), which enables detection of the gene copies through an immunoperoxidase reaction. CISH was performed in 79 breast carcinomas for which the HER2 status was previously determined by IHC and FISH. The results of IHC, FISH and CISH were compared for each tumour. CISH procedures were successful in 95% of our cases. Whatever the IHC results, we found a very good concordance (96%) between CISH and FISH. Our study confirms that CISH may be an alternative to FISH for the determination of the gene amplification status in 2+ tumours. Our results allow us to think that, in many laboratories, CISH may also be an excellent method to calibrate the IHC procedures or, as a quality control test, to check regularly that the IHC signal is in agreement with the gene status.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/sj.bjc.6600943", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1017082", 
        "issn": [
          "0007-0920", 
          "1532-1827"
        ], 
        "name": "British Journal of Cancer", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "88"
      }
    ], 
    "name": "Agreement between chromogenic in situ hybridisation (CISH) and FISH in the determination of HER2 status in breast cancer", 
    "pagination": "6600943", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "aee5bae4caaa62891d87bfe8e6f31756d570462b0d6a33cd7c4c76c5b842211b"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12771927"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0370635"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/sj.bjc.6600943"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036715126"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/sj.bjc.6600943", 
      "https://app.dimensions.ai/details/publication/pub.1036715126"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29194_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/6600943"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6600943'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6600943'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6600943'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/sj.bjc.6600943'


 

This table displays all metadata directly associated to this object as RDF triples.

290 TRIPLES      21 PREDICATES      71 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/sj.bjc.6600943 schema:about N123c1745554e4d5e9b494849689012ea
2 N165423d16bf545bb85276b3e5179c696
3 N2994687498484ba69098c9ad4357eed3
4 N90afb534d6e149c69bdaa6ee0384cffe
5 N980a0855b12844acb43c3e6b1f2d0bcd
6 N98f506d865aa4119b2b59e0e1687620a
7 Nbd5c02cca5a2489abe3c9d88e78b1bb6
8 Nbf5b6edbb2c1449da300d063e9e72241
9 Nc318fa7c6c2b484b955f1469044df882
10 Nc8d17196018344778c6a74a77dbb6d80
11 Ne45c5c1aaab044f2a59d66478b2e8d14
12 anzsrc-for:11
13 anzsrc-for:1112
14 schema:author N61aa524edc0b404b828d29b7384bddb9
15 schema:citation sg:pub.10.1038/modpathol.3880007
16 sg:pub.10.1038/modpathol.3880154
17 sg:pub.10.1038/modpathol.3880228
18 sg:pub.10.1038/modpathol.3880582
19 https://app.dimensions.ai/details/publication/pub.1074665592
20 https://app.dimensions.ai/details/publication/pub.1074889449
21 https://app.dimensions.ai/details/publication/pub.1082561095
22 https://app.dimensions.ai/details/publication/pub.1083122347
23 https://doi.org/10.1002/path.971
24 https://doi.org/10.1016/s0002-9440(10)64785-2
25 https://doi.org/10.1016/s0093-7754(01)90281-7
26 https://doi.org/10.1046/j.1365-2559.2003.01598.x
27 https://doi.org/10.1053/sonc.2001.28549
28 https://doi.org/10.1056/nejm200103153441101
29 https://doi.org/10.1073/pnas.89.12.5321
30 https://doi.org/10.1093/annonc/12.suppl_1.s101
31 https://doi.org/10.1093/jnci/94.11.852
32 https://doi.org/10.1093/jnci/94.11.855
33 https://doi.org/10.1177/107327480100800504
34 https://doi.org/10.1200/jco.1998.16.8.2659
35 https://doi.org/10.1200/jco.1999.17.1.434
36 https://doi.org/10.1200/jco.1999.17.7.1974
37 https://doi.org/10.1200/jco.1999.17.9.2639
38 https://doi.org/10.1200/jco.20.3.719
39 https://doi.org/10.1200/jco.2000.18.21.3651
40 https://doi.org/10.1200/jco.2001.19.10.2714
41 https://doi.org/10.1200/jco.2001.19.2.354
42 https://doi.org/10.1200/jco.2002.20.3.719
43 https://doi.org/10.1309/980m-e24r-v19k-595d
44 https://doi.org/10.1309/aj84-50ak-1x1b-1q4c
45 https://doi.org/10.1309/vacp-vlqa-g9dx-vudf
46 schema:datePublished 2003-05
47 schema:datePublishedReg 2003-05-01
48 schema:description Determination of the HER2/neu (HER2) status in breast carcinoma has become necessary for the selection of breast cancer patients for trastuzumab therapy. Amplification of the gene analysed by fluorescence in situ hybridisation (FISH) or overexpression of the protein determined by immunohistochemistry (IHC) are the two major methods to establish this status. A strong correlation has been previously demonstrated between these two methods. However, FISH is not always feasible in routine practice and weakly positive IHC tumours (2+) do not always correspond to a gene amplification. Our study was performed in order to evaluate the contribution of chromogenic in situ hybridisation (CISH), which enables detection of the gene copies through an immunoperoxidase reaction. CISH was performed in 79 breast carcinomas for which the HER2 status was previously determined by IHC and FISH. The results of IHC, FISH and CISH were compared for each tumour. CISH procedures were successful in 95% of our cases. Whatever the IHC results, we found a very good concordance (96%) between CISH and FISH. Our study confirms that CISH may be an alternative to FISH for the determination of the gene amplification status in 2+ tumours. Our results allow us to think that, in many laboratories, CISH may also be an excellent method to calibrate the IHC procedures or, as a quality control test, to check regularly that the IHC signal is in agreement with the gene status.
49 schema:genre research_article
50 schema:inLanguage en
51 schema:isAccessibleForFree true
52 schema:isPartOf N9af9f8122b784f748653a117225e94ee
53 Nfa8380056d0340f8b22d704e65832daa
54 sg:journal.1017082
55 schema:name Agreement between chromogenic in situ hybridisation (CISH) and FISH in the determination of HER2 status in breast cancer
56 schema:pagination 6600943
57 schema:productId Na33cb95b979945d2bcc3b327ebed2da0
58 Na41871a41d6b45908dd259b43ee9c2fe
59 Nb74368169d77436695e70c621b90cbc9
60 Ne58da8832c014c9e80767049a96787f8
61 Nf971e284e31a403198dc24364258519c
62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036715126
63 https://doi.org/10.1038/sj.bjc.6600943
64 schema:sdDatePublished 2019-04-11T11:52
65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
66 schema:sdPublisher N9f0048f93904489d899011e5165c386d
67 schema:url http://www.nature.com/articles/6600943
68 sgo:license sg:explorer/license/
69 sgo:sdDataset articles
70 rdf:type schema:ScholarlyArticle
71 N123c1745554e4d5e9b494849689012ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Gene Amplification
73 rdf:type schema:DefinedTerm
74 N165423d16bf545bb85276b3e5179c696 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Humans
76 rdf:type schema:DefinedTerm
77 N2994687498484ba69098c9ad4357eed3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Breast Neoplasms
79 rdf:type schema:DefinedTerm
80 N37734250f37a4b2893a7418bac2b7dc4 rdf:first sg:person.01210131470.86
81 rdf:rest Na53ac0b7c74c4a3da158d35cb3688da4
82 N4879d293561b4bf99c4c1f9efc3c93c5 rdf:first sg:person.01021320600.66
83 rdf:rest Ncfbd571c3f2d49b9ae223eae6d54e3d6
84 N5398d1287bd84ca2b46b05f640ad10c6 rdf:first sg:person.0706637557.98
85 rdf:rest N37734250f37a4b2893a7418bac2b7dc4
86 N589b0377224f42438e0c03a65951115a schema:name Centre O Lambret, Lille, France
87 rdf:type schema:Organization
88 N61aa524edc0b404b828d29b7384bddb9 rdf:first sg:person.01121675220.27
89 rdf:rest N4879d293561b4bf99c4c1f9efc3c93c5
90 N8238fd47282c4097bbf576f8014ba829 rdf:first sg:person.0643603315.57
91 rdf:rest N5398d1287bd84ca2b46b05f640ad10c6
92 N90afb534d6e149c69bdaa6ee0384cffe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Sensitivity and Specificity
94 rdf:type schema:DefinedTerm
95 N980a0855b12844acb43c3e6b1f2d0bcd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Tumor Cells, Cultured
97 rdf:type schema:DefinedTerm
98 N980f6a91e15d4832851b6b469f4751ba schema:name Institut Bergognie, Bordeaux, France
99 rdf:type schema:Organization
100 N98f506d865aa4119b2b59e0e1687620a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name In Situ Hybridization
102 rdf:type schema:DefinedTerm
103 N9af9f8122b784f748653a117225e94ee schema:issueNumber 10
104 rdf:type schema:PublicationIssue
105 N9f0048f93904489d899011e5165c386d schema:name Springer Nature - SN SciGraph project
106 rdf:type schema:Organization
107 Na33cb95b979945d2bcc3b327ebed2da0 schema:name nlm_unique_id
108 schema:value 0370635
109 rdf:type schema:PropertyValue
110 Na41871a41d6b45908dd259b43ee9c2fe schema:name pubmed_id
111 schema:value 12771927
112 rdf:type schema:PropertyValue
113 Na53ac0b7c74c4a3da158d35cb3688da4 rdf:first sg:person.012211445072.32
114 rdf:rest Nde45f9316d9f4449b78b1b5d8259f761
115 Nb74368169d77436695e70c621b90cbc9 schema:name dimensions_id
116 schema:value pub.1036715126
117 rdf:type schema:PropertyValue
118 Nbd5c02cca5a2489abe3c9d88e78b1bb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Gene Expression Regulation
120 rdf:type schema:DefinedTerm
121 Nbf5b6edbb2c1449da300d063e9e72241 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name In Situ Hybridization, Fluorescence
123 rdf:type schema:DefinedTerm
124 Nc318fa7c6c2b484b955f1469044df882 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Immunohistochemistry
126 rdf:type schema:DefinedTerm
127 Nc8d17196018344778c6a74a77dbb6d80 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Genes, erbB-2
129 rdf:type schema:DefinedTerm
130 Ncd815a02069b407b9a398ae029eb5530 schema:name Centre J Perrin, Clermont-Ferrand, France
131 rdf:type schema:Organization
132 Ncfbd571c3f2d49b9ae223eae6d54e3d6 rdf:first sg:person.01253370320.32
133 rdf:rest N8238fd47282c4097bbf576f8014ba829
134 Nde4071293d2f4332b057d6ccceea4037 rdf:first sg:person.0760136540.00
135 rdf:rest rdf:nil
136 Nde45f9316d9f4449b78b1b5d8259f761 rdf:first sg:person.01306222052.48
137 rdf:rest Ne9c1667f03cb4431a7716c35b9acecac
138 Ne45c5c1aaab044f2a59d66478b2e8d14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Female
140 rdf:type schema:DefinedTerm
141 Ne58da8832c014c9e80767049a96787f8 schema:name doi
142 schema:value 10.1038/sj.bjc.6600943
143 rdf:type schema:PropertyValue
144 Ne9c1667f03cb4431a7716c35b9acecac rdf:first sg:person.01317343552.06
145 rdf:rest Nde4071293d2f4332b057d6ccceea4037
146 Nf971e284e31a403198dc24364258519c schema:name readcube_id
147 schema:value aee5bae4caaa62891d87bfe8e6f31756d570462b0d6a33cd7c4c76c5b842211b
148 rdf:type schema:PropertyValue
149 Nfa8380056d0340f8b22d704e65832daa schema:volumeNumber 88
150 rdf:type schema:PublicationVolume
151 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
152 schema:name Medical and Health Sciences
153 rdf:type schema:DefinedTerm
154 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
155 schema:name Oncology and Carcinogenesis
156 rdf:type schema:DefinedTerm
157 sg:journal.1017082 schema:issn 0007-0920
158 1532-1827
159 schema:name British Journal of Cancer
160 rdf:type schema:Periodical
161 sg:person.01021320600.66 schema:affiliation https://www.grid.ac/institutes/grid.418189.d
162 schema:familyName Denoux
163 schema:givenName Y
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021320600.66
165 rdf:type schema:Person
166 sg:person.01121675220.27 schema:affiliation https://www.grid.ac/institutes/grid.418037.9
167 schema:familyName Arnould
168 schema:givenName L
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121675220.27
170 rdf:type schema:Person
171 sg:person.01210131470.86 schema:affiliation https://www.grid.ac/institutes/grid.418116.b
172 schema:familyName Treilleux
173 schema:givenName I
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210131470.86
175 rdf:type schema:Person
176 sg:person.012211445072.32 schema:affiliation https://www.grid.ac/institutes/grid.14925.3b
177 schema:familyName Mathieu
178 schema:givenName M C
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012211445072.32
180 rdf:type schema:Person
181 sg:person.01253370320.32 schema:affiliation N980f6a91e15d4832851b6b469f4751ba
182 schema:familyName MacGrogan
183 schema:givenName G
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253370320.32
185 rdf:type schema:Person
186 sg:person.01306222052.48 schema:affiliation https://www.grid.ac/institutes/grid.418596.7
187 schema:familyName Vincent-Salomon
188 schema:givenName A
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306222052.48
190 rdf:type schema:Person
191 sg:person.01317343552.06 schema:affiliation N589b0377224f42438e0c03a65951115a
192 schema:familyName Vilain
193 schema:givenName M O
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317343552.06
195 rdf:type schema:Person
196 sg:person.0643603315.57 schema:affiliation Ncd815a02069b407b9a398ae029eb5530
197 schema:familyName Penault-Llorca
198 schema:givenName F
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643603315.57
200 rdf:type schema:Person
201 sg:person.0706637557.98 schema:affiliation https://www.grid.ac/institutes/grid.277151.7
202 schema:familyName Fiche
203 schema:givenName M
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706637557.98
205 rdf:type schema:Person
206 sg:person.0760136540.00 schema:affiliation https://www.grid.ac/institutes/grid.418596.7
207 schema:familyName Couturier
208 schema:givenName J
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760136540.00
210 rdf:type schema:Person
211 sg:pub.10.1038/modpathol.3880007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011441671
212 https://doi.org/10.1038/modpathol.3880007
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/modpathol.3880154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033626551
215 https://doi.org/10.1038/modpathol.3880154
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/modpathol.3880228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032076676
218 https://doi.org/10.1038/modpathol.3880228
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/modpathol.3880582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030675311
221 https://doi.org/10.1038/modpathol.3880582
222 rdf:type schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1074665592 schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1074889449 schema:CreativeWork
225 https://app.dimensions.ai/details/publication/pub.1082561095 schema:CreativeWork
226 https://app.dimensions.ai/details/publication/pub.1083122347 schema:CreativeWork
227 https://doi.org/10.1002/path.971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017034982
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/s0002-9440(10)64785-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023575302
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/s0093-7754(01)90281-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049670670
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1046/j.1365-2559.2003.01598.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034591528
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1053/sonc.2001.28549 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057078448
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1056/nejm200103153441101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031768583
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1073/pnas.89.12.5321 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011077656
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1093/annonc/12.suppl_1.s101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044332874
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1093/jnci/94.11.852 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010730561
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1093/jnci/94.11.855 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015062444
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1177/107327480100800504 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074887583
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1200/jco.1998.16.8.2659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083305984
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1200/jco.1999.17.1.434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074511260
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1200/jco.1999.17.7.1974 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074549797
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1200/jco.1999.17.9.2639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074549894
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1200/jco.20.3.719 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064202789
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1200/jco.2000.18.21.3651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074719576
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1200/jco.2001.19.10.2714 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074814016
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1200/jco.2001.19.2.354 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074768320
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1200/jco.2002.20.3.719 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083941918
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1309/980m-e24r-v19k-595d schema:sameAs https://app.dimensions.ai/details/publication/pub.1044347783
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1309/aj84-50ak-1x1b-1q4c schema:sameAs https://app.dimensions.ai/details/publication/pub.1053290441
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1309/vacp-vlqa-g9dx-vudf schema:sameAs https://app.dimensions.ai/details/publication/pub.1014241811
272 rdf:type schema:CreativeWork
273 https://www.grid.ac/institutes/grid.14925.3b schema:alternateName Institut Gustave Roussy
274 schema:name Institut G Roussy, Villejuif, France
275 rdf:type schema:Organization
276 https://www.grid.ac/institutes/grid.277151.7 schema:alternateName Centre Hospitalier Universitaire de Nantes
277 schema:name Hôpital G et R Laennec, Nantes, France
278 rdf:type schema:Organization
279 https://www.grid.ac/institutes/grid.418037.9 schema:alternateName Centre Georges François Leclerc
280 schema:name Department of Pathology, Centre GF Leclerc, 1 rue Pr Marion, 21034 Dijon cedex, France
281 rdf:type schema:Organization
282 https://www.grid.ac/institutes/grid.418116.b schema:alternateName Centre Léon Bérard
283 schema:name Centre L Berard, Lyon, France
284 rdf:type schema:Organization
285 https://www.grid.ac/institutes/grid.418189.d schema:alternateName UniCancer Group
286 schema:name Department of Pathology, Centre F Baclesse, Caen, France
287 rdf:type schema:Organization
288 https://www.grid.ac/institutes/grid.418596.7 schema:alternateName Institute Curie
289 schema:name Institut Curie, Paris, France
290 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...