Protocol for differentiating human stem cells into CD34+ progenitor cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-10

ABSTRACT

Studies in mice and in cell culture suggest that inhibition of MAP kinase kinase (MEK) plus activation of bone morphogenetic protein 4 (BMP4) could help differentiate human stem cells into CD34+ progenitor cells to treat cardiovascular diseases. In human embryonic and induced pluripotent stem cells, a MEK inhibitor plus a BMP4 activator led to differentiation of cells into mesodermal CD34+ progenitor cells that subsequently differentiated into endothelial cells. In a mouse model of ischemic limb injury, intramuscular injection of the stem cell–derived CD34+ progenitor cells improved recovery compared with injection of stem cell–derived CD34− cells or vehicle. Next steps could include evaluating the stem cell–derived CD34+ progenitor cells in additional mouse models of cardiovascular disease. More... »

PAGES

1251-1251

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/scibx.2010.1251

DOI

http://dx.doi.org/10.1038/scibx.2010.1251

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028083964


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