Improved visual detection of DNA amplification using pyridylazophenol metal sensing dyes View Full Text


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Article Info

DATE

2022-09-21

AUTHORS

Yinhua Zhang, Eric A. Hunt, Esta Tamanaha, Ivan R. Corrêa, Nathan A. Tanner

ABSTRACT

Detection of nucleic acid amplification has typically required sophisticated laboratory instrumentation, but as the amplification techniques have moved away from the lab, complementary detection techniques have been implemented to facilitate point-of-care, field, and even at-home applications. Simple visual detection approaches have been widely used for isothermal amplification methods, but have generally displayed weak color changes or been highly sensitive to sample and atmospheric effects. Here we describe the use of pyridylazophenol dyes and binding to manganese ion to produce a strong visible color that changes in response to nucleic acid amplification. This detection approach is easily quantitated with absorbance, rapidly and clearly visible by eye, robust to sample effects, and notably compatible with both isothermal and PCR amplification. Nucleic acid amplification and molecular diagnostic methods are being used in an increasing number of novel applications and settings, and the ability to reliably and sensitively detect them without the need for additional instrumentation will enable even more access to these powerful techniques. More... »

PAGES

999

References to SciGraph publications

  • 2020-12-16. A rapid colorimetric LAMP assay for detection of Rhizoctonia solani AG-1 IA causing sheath blight of rice in SCIENTIFIC REPORTS
  • 2002-02. Characteristics and applications of nucleic acid sequence-based amplification (NASBA) in MOLECULAR BIOTECHNOLOGY
  • 2020-09-15. Quantification of colorimetric isothermal amplification on the smartphone and its open-source app for point-of-care pathogen detection in SCIENTIFIC REPORTS
  • 2019-03-20. A rapid, sensitive and inexpensive method for detection of grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification in ARCHIVES OF VIROLOGY
  • 1985-12-23. Water Soluble Pyridylazoaminophenols and Pyridylazoamino-benzoic Acids as Highly Sensitive Photometric Reagents for Zinc, Uranium, Cobalt and Nickel in ANALYTICAL SCIENCES
  • 2021-08-12. A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples in SCIENTIFIC REPORTS
  • 2008-04-24. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products in NATURE PROTOCOLS
  • 2020-12-09. Digital postprocessing and image segmentation for objective analysis of colorimetric reactions in NATURE PROTOCOLS
  • 2017-01-06. Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay in SCIENTIFIC REPORTS
  • 2019-08-15. Detecting wMel Wolbachia in field-collected Aedes aegypti mosquitoes using loop-mediated isothermal amplification (LAMP) in PARASITES & VECTORS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s42003-022-03973-x

    DOI

    http://dx.doi.org/10.1038/s42003-022-03973-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1151183049

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/36130997


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